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The map label for this gene is aegA [H]

Identifier: 239618192

GI number: 239618192

Start: 1956202

End: 1957602

Strand: Direct

Name: aegA [H]

Synonym: Kole_1828

Alternate gene names: 239618192

Gene position: 1956202-1957602 (Clockwise)

Preceding gene: 239618191

Following gene: 239618193

Centisome position: 84.97

GC content: 47.47

Gene sequence:

>1401_bases
GTGAGCAGACCCAAGAAAGTTCCGATGCCTGAATTACCACCACACGAAAGAATAGTGTCTTTTAAAGAAGTTGCTTTAGG
GTACACAGAAGAGCAGGCTGTTGTAGAAGCCCAGCGTTGCCTTCAGTGTAAAGTACCACTGTGTATTCAAGGATGCCCCG
TTGGTATTGATATTCCCGGATTCATCCGAGCCATAAGCGAAAGGGATTTTGACAGTGGAATAAAGATATTAAAGGATAGC
AACAGCTTACCCGCGATCACAGGGAGAGTATGTCCACAGGAAAAGCAATGTGAGGCAAAATGCGTGCTTGGAAAGATCAA
AGGGACTGAACCGGTAGCCATAGGCAGGATGGAGAGGTTTCTGGCGGATATGGATCTCAAAGCCTGCCCTAAATCGGTGC
AACGGGTGAGGAAAAACAAAGGTAATGTGGCGGTTATCGGAGCGGGGCCCGCGGGACTTACCGTTGCCGGCGACCTGGCG
AAACTCGGATATGAGGTAACGGTTTTTGAGGCTTTTCACAAGGCCGGTGGAGTGCTTGTTTATGGAATCCCGGAATTCAG
GCTTCCAAAAGATATAGTACAGAAGGAAGTCGAGTATGTTCAATCGCTGGGAGTCAAATTTGTTTACAATCAAATCATCG
GAAGAACTATTCCTTTTGAAGAATTGAGGAATGAGTATGACGCTATATTTATAGGCATCGGTGCCGGGGCACCAAGATTC
ATGGGGATACCCGGTTCGAATCTCAATAACATATACTCGGCCAGTGAATTTCTGACACGTGTTAACTTAATGAGGGCAAA
CCGGTTTCCTGAGTACGATACACCCGTTAAGATTTCCGATAGGGTTGCGGTTATCGGTGCAGGGAACGTCTCCATGGATG
CCGCAAGGACAGCCAAAAGGCTCGGTGCAAAAGAGGTTACCATAATCTACCGCAGGAGTGAAGCCGAAATGCCTGCAAGG
CTTGAAGAATATCACCATGCCCTTGAAGAAGAGATAAAATTCTTCTGGCTAACACAACCGGTGGAATACATTGGCGACAT
AAACAACAATGTCAGGAAAATGAGATGTGTCAGGATGGAATTGGGATCTCCGGATGAATCAGGTCGAAGAAGACCGATTC
CCATTGAGGGATCGGAGTTCGAGATAGAGATCGATCTTGTCATAGAAGCCATAGGCCAAAAAGCCAATGCTGTTTTGAAA
ACAGGCTTTCCGGGATTGAAACTCAATCGTTGGGGTTATATCGATGCTGATCCCGAGACAGGTCAGACCAATCTTGAAGG
CGTTTTTGCAGGAGGCGACATAGTAACAGGAGCCGCAACGGTTATAGAAGCCATGGGTGCGGGGAAAAAGGCTGCTAAGG
CGATTGGGGAGTATCTGCTGAAAGCCAGCAAAGCTGGCTGA

Upstream 100 bases:

>100_bases
TCAACTGGAACGAATTGATAAAACGCCAGCATCAATACATGGAAGAAGAAAGAAAAGCGCTTGAGGCTTTTAAGAAAAAG
GCTGGTGATCTCTCATGGCT

Downstream 100 bases:

>100_bases
AGTGCTGGACTACGTCCAGGAGGCTCACGACGTTGTCAGTTGATGGAGCGATGCGAACCGCAAGCGGTTGTCACGAACTC
CTGGTAAGAATCGAGAGTCC

Product: glutamate synthase (NADPH), homotetrameric

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSRPKKVPMPELPPHERIVSFKEVALGYTEEQAVVEAQRCLQCKVPLCIQGCPVGIDIPGFIRAISERDFDSGIKILKDS
NSLPAITGRVCPQEKQCEAKCVLGKIKGTEPVAIGRMERFLADMDLKACPKSVQRVRKNKGNVAVIGAGPAGLTVAGDLA
KLGYEVTVFEAFHKAGGVLVYGIPEFRLPKDIVQKEVEYVQSLGVKFVYNQIIGRTIPFEELRNEYDAIFIGIGAGAPRF
MGIPGSNLNNIYSASEFLTRVNLMRANRFPEYDTPVKISDRVAVIGAGNVSMDAARTAKRLGAKEVTIIYRRSEAEMPAR
LEEYHHALEEEIKFFWLTQPVEYIGDINNNVRKMRCVRMELGSPDESGRRRPIPIEGSEFEIEIDLVIEAIGQKANAVLK
TGFPGLKLNRWGYIDADPETGQTNLEGVFAGGDIVTGAATVIEAMGAGKKAAKAIGEYLLKASKAG

Sequences:

>Translated_466_residues
MSRPKKVPMPELPPHERIVSFKEVALGYTEEQAVVEAQRCLQCKVPLCIQGCPVGIDIPGFIRAISERDFDSGIKILKDS
NSLPAITGRVCPQEKQCEAKCVLGKIKGTEPVAIGRMERFLADMDLKACPKSVQRVRKNKGNVAVIGAGPAGLTVAGDLA
KLGYEVTVFEAFHKAGGVLVYGIPEFRLPKDIVQKEVEYVQSLGVKFVYNQIIGRTIPFEELRNEYDAIFIGIGAGAPRF
MGIPGSNLNNIYSASEFLTRVNLMRANRFPEYDTPVKISDRVAVIGAGNVSMDAARTAKRLGAKEVTIIYRRSEAEMPAR
LEEYHHALEEEIKFFWLTQPVEYIGDINNNVRKMRCVRMELGSPDESGRRRPIPIEGSEFEIEIDLVIEAIGQKANAVLK
TGFPGLKLNRWGYIDADPETGQTNLEGVFAGGDIVTGAATVIEAMGAGKKAAKAIGEYLLKASKAG
>Mature_465_residues
SRPKKVPMPELPPHERIVSFKEVALGYTEEQAVVEAQRCLQCKVPLCIQGCPVGIDIPGFIRAISERDFDSGIKILKDSN
SLPAITGRVCPQEKQCEAKCVLGKIKGTEPVAIGRMERFLADMDLKACPKSVQRVRKNKGNVAVIGAGPAGLTVAGDLAK
LGYEVTVFEAFHKAGGVLVYGIPEFRLPKDIVQKEVEYVQSLGVKFVYNQIIGRTIPFEELRNEYDAIFIGIGAGAPRFM
GIPGSNLNNIYSASEFLTRVNLMRANRFPEYDTPVKISDRVAVIGAGNVSMDAARTAKRLGAKEVTIIYRRSEAEMPARL
EEYHHALEEEIKFFWLTQPVEYIGDINNNVRKMRCVRMELGSPDESGRRRPIPIEGSEFEIEIDLVIEAIGQKANAVLKT
GFPGLKLNRWGYIDADPETGQTNLEGVFAGGDIVTGAATVIEAMGAGKKAAKAIGEYLLKASKAG

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=465, Percent_Identity=32.4731182795699, Blast_Score=202, Evalue=7e-52,
Organism=Escherichia coli, GI1788811, Length=450, Percent_Identity=37.1111111111111, Blast_Score=288, Evalue=6e-79,
Organism=Escherichia coli, GI87082180, Length=448, Percent_Identity=38.1696428571429, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1789606, Length=463, Percent_Identity=34.7732181425486, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1788468, Length=444, Percent_Identity=31.981981981982, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1789243, Length=420, Percent_Identity=34.0476190476191, Blast_Score=203, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI71984108, Length=453, Percent_Identity=31.1258278145695, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17570289, Length=482, Percent_Identity=28.2157676348548, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17543860, Length=175, Percent_Identity=33.1428571428571, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=478, Percent_Identity=30.5439330543933, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24665547, Length=481, Percent_Identity=29.9376299376299, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24665543, Length=481, Percent_Identity=29.9376299376299, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI28574881, Length=481, Percent_Identity=29.9376299376299, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24665539, Length=481, Percent_Identity=29.9376299376299, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24640763, Length=449, Percent_Identity=28.9532293986637, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI18858217, Length=449, Percent_Identity=28.9532293986637, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 51030; Mature: 50899

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPKKVPMPELPPHERIVSFKEVALGYTEEQAVVEAQRCLQCKVPLCIQGCPVGIDIPG
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHH
FIRAISERDFDSGIKILKDSNSLPAITGRVCPQEKQCEAKCVLGKIKGTEPVAIGRMERF
HHHHHHHCCCCCCCEEEECCCCCCEEECCCCCCCCCCCHHEEEEECCCCCCCHHHHHHHH
LADMDLKACPKSVQRVRKNKGNVAVIGAGPAGLTVAGDLAKLGYEVTVFEAFHKAGGVLV
HHHCCHHHCHHHHHHHHHCCCCEEEEECCCCCCEEECHHHHCCCEEEEHHHHHHHCCEEE
YGIPEFRLPKDIVQKEVEYVQSLGVKFVYNQIIGRTIPFEELRNEYDAIFIGIGAGAPRF
ECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHCCCCCEEEEECCCCCCCE
MGIPGSNLNNIYSASEFLTRVNLMRANRFPEYDTPVKISDRVAVIGAGNVSMDAARTAKR
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEECCCCCHHHHHHHHH
LGAKEVTIIYRRSEAEMPARLEEYHHALEEEIKFFWLTQPVEYIGDINNNVRKMRCVRME
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHCCCCCHHHHHEEEEC
LGSPDESGRRRPIPIEGSEFEIEIDLVIEAIGQKANAVLKTGFPGLKLNRWGYIDADPET
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCHHCEEEECCCCCCEECCCEEECCCCCC
GQTNLEGVFAGGDIVTGAATVIEAMGAGKKAAKAIGEYLLKASKAG
CCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRPKKVPMPELPPHERIVSFKEVALGYTEEQAVVEAQRCLQCKVPLCIQGCPVGIDIPG
CCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHH
FIRAISERDFDSGIKILKDSNSLPAITGRVCPQEKQCEAKCVLGKIKGTEPVAIGRMERF
HHHHHHHCCCCCCCEEEECCCCCCEEECCCCCCCCCCCHHEEEEECCCCCCCHHHHHHHH
LADMDLKACPKSVQRVRKNKGNVAVIGAGPAGLTVAGDLAKLGYEVTVFEAFHKAGGVLV
HHHCCHHHCHHHHHHHHHCCCCEEEEECCCCCCEEECHHHHCCCEEEEHHHHHHHCCEEE
YGIPEFRLPKDIVQKEVEYVQSLGVKFVYNQIIGRTIPFEELRNEYDAIFIGIGAGAPRF
ECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHCCCCCEEEEECCCCCCCE
MGIPGSNLNNIYSASEFLTRVNLMRANRFPEYDTPVKISDRVAVIGAGNVSMDAARTAKR
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEECCCCCHHHHHHHHH
LGAKEVTIIYRRSEAEMPARLEEYHHALEEEIKFFWLTQPVEYIGDINNNVRKMRCVRME
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHCCCCCHHHHHEEEEC
LGSPDESGRRRPIPIEGSEFEIEIDLVIEAIGQKANAVLKTGFPGLKLNRWGYIDADPET
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCHHCEEEECCCCCCEECCCEEECCCCCC
GQTNLEGVFAGGDIVTGAATVIEAMGAGKKAAKAIGEYLLKASKAG
CCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]