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The map label for this gene is ispD [H]

Identifier: 239618152

GI number: 239618152

Start: 1915113

End: 1915820

Strand: Direct

Name: ispD [H]

Synonym: Kole_1785

Alternate gene names: 239618152

Gene position: 1915113-1915820 (Clockwise)

Preceding gene: 239618151

Following gene: 239618153

Centisome position: 83.19

GC content: 40.68

Gene sequence:

>708_bases
ATGAGAATAAAGGTAGCGGCCGTCATAGTTGCTGGTGGTAAGGGAACACGCTTTGGCGCGAATGTTCCTAAACAATTTAT
CAAGCTTGAAGGAAAGGAAATATTTGTGAGATCCGTGGAAATCTTCCACGGGTCTCCTTTTATTTCACATACAGTTCTTG
TGATGAATCCAGACTGGATTGACAAAGCAAAAGAGATTCTTTCTTGTTATGAATTGGATGATGTCGTAGTAGTTTCTGGT
GGTGAAACACGTCAGCTGTCAGTATTGAATGGTCTTAAAGCTCTTGAGCCTGTCAAACCTGACATTGTGATGATTCATGA
TGCGGCACGCCCGCTTTTCGAGAAAAATTGCATTCCTAAAATTATTGCTTCAGTTGAACCCGAAATTGGTGTTGTTGTAA
CTGAACCCGCAAAAGAAACTCTATATATCGTAAAAGATGGTTTTGTGGAGAAAGTTCCGGACAGATCTTTTTACTGGCAC
GCTAAAACCCCTCAGAGTTTCTTCTTCGCAGAAATTCTTGAAGCTCATCTTCACGCACTGGAACAGGGAATAACCAACGC
TACCGATGACTCTCAGCTTTTCCTCAGAGTTGGCAAAAAAGTAAAAATTCTTGAATGTGGCGGGAAAAACCCTAAAATAA
CTACTGCTCAAGATTTGGCAGAAGCTTCGTGTGTATTAAAATTAAATAAACCGAGATCCGAGAATTGA

Upstream 100 bases:

>100_bases
ATGAGCTCATGACGGTGGCGGATAAGGGCATGATAATGCCACCAAAATCCACATGGTTTGAACCAAAACTCAGAAGTGGA
CTCTTCGTGCATTTGTTGAA

Downstream 100 bases:

>100_bases
GAAACCGAGAACCGGTAGCTGCTGCGCAGCGACAAGAATCCCTTCGGGATTGACACGACCAGCTACGCCGGTGTTACGAA
CTCCTTTGGAGTTGTTCCGA

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MRIKVAAVIVAGGKGTRFGANVPKQFIKLEGKEIFVRSVEIFHGSPFISHTVLVMNPDWIDKAKEILSCYELDDVVVVSG
GETRQLSVLNGLKALEPVKPDIVMIHDAARPLFEKNCIPKIIASVEPEIGVVVTEPAKETLYIVKDGFVEKVPDRSFYWH
AKTPQSFFFAEILEAHLHALEQGITNATDDSQLFLRVGKKVKILECGGKNPKITTAQDLAEASCVLKLNKPRSEN

Sequences:

>Translated_235_residues
MRIKVAAVIVAGGKGTRFGANVPKQFIKLEGKEIFVRSVEIFHGSPFISHTVLVMNPDWIDKAKEILSCYELDDVVVVSG
GETRQLSVLNGLKALEPVKPDIVMIHDAARPLFEKNCIPKIIASVEPEIGVVVTEPAKETLYIVKDGFVEKVPDRSFYWH
AKTPQSFFFAEILEAHLHALEQGITNATDDSQLFLRVGKKVKILECGGKNPKITTAQDLAEASCVLKLNKPRSEN
>Mature_235_residues
MRIKVAAVIVAGGKGTRFGANVPKQFIKLEGKEIFVRSVEIFHGSPFISHTVLVMNPDWIDKAKEILSCYELDDVVVVSG
GETRQLSVLNGLKALEPVKPDIVMIHDAARPLFEKNCIPKIIASVEPEIGVVVTEPAKETLYIVKDGFVEKVPDRSFYWH
AKTPQSFFFAEILEAHLHALEQGITNATDDSQLFLRVGKKVKILECGGKNPKITTAQDLAEASCVLKLNKPRSEN

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=240, Percent_Identity=28.3333333333333, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI157671913, Length=127, Percent_Identity=32.2834645669291, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1789104, Length=228, Percent_Identity=31.5789473684211, Blast_Score=94, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 25978; Mature: 25978

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU ; PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIKVAAVIVAGGKGTRFGANVPKQFIKLEGKEIFVRSVEIFHGSPFISHTVLVMNPDWI
CEEEEEEEEEECCCCCCCCCCCCHHHHHCCCCEEEEEEEEEECCCCCHHEEEEEECCCHH
DKAKEILSCYELDDVVVVSGGETRQLSVLNGLKALEPVKPDIVMIHDAARPLFEKNCIPK
HHHHHHHHHHCCCCEEEECCCCCEEEHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCHHH
IIASVEPEIGVVVTEPAKETLYIVKDGFVEKVPDRSFYWHAKTPQSFFFAEILEAHLHAL
HHHCCCCCCEEEEECCCCCEEEEEECCCHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHH
EQGITNATDDSQLFLRVGKKVKILECGGKNPKITTAQDLAEASCVLKLNKPRSEN
HHCCCCCCCCHHHHHHCCCEEEEEEECCCCCCEECHHHHHHCEEEEEECCCCCCC
>Mature Secondary Structure
MRIKVAAVIVAGGKGTRFGANVPKQFIKLEGKEIFVRSVEIFHGSPFISHTVLVMNPDWI
CEEEEEEEEEECCCCCCCCCCCCHHHHHCCCCEEEEEEEEEECCCCCHHEEEEEECCCHH
DKAKEILSCYELDDVVVVSGGETRQLSVLNGLKALEPVKPDIVMIHDAARPLFEKNCIPK
HHHHHHHHHHCCCCEEEECCCCCEEEHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCHHH
IIASVEPEIGVVVTEPAKETLYIVKDGFVEKVPDRSFYWHAKTPQSFFFAEILEAHLHAL
HHHCCCCCCEEEEECCCCCEEEEEECCCHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHH
EQGITNATDDSQLFLRVGKKVKILECGGKNPKITTAQDLAEASCVLKLNKPRSEN
HHCCCCCCCCHHHHHHCCCEEEEEEECCCCCCEECHHHHHHCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA