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The map label for this gene is mrpA [H]

Identifier: 239618146

GI number: 239618146

Start: 1908000

End: 1909433

Strand: Direct

Name: mrpA [H]

Synonym: Kole_1779

Alternate gene names: 239618146

Gene position: 1908000-1909433 (Clockwise)

Preceding gene: 239618145

Following gene: 239618148

Centisome position: 82.88

GC content: 41.35

Gene sequence:

>1434_bases
ATGATACTTTACATAAGCCTTATTCCGCTTTTTCTCGGTGGCCTATCGCTTATACTTAATCGTTATAGAAAAATAACCAT
AACCTTGACGTTCGTATCCTTGATATCTTCAGCATTAATACTGATCCTCAATATCGGTAACAGTTACGAGACAGTGATAG
GAAATTGGGGAACGTTAGGTATATCCATAGGATACGATGAAATAGCTTTGCCTTTCCTTCTTGCTGTAGTGGTAGTTCTT
TTTGCTGTAACTTTGAATTCCCTTCACAAGGATTATGATGGAATTTTTTATGCTCTCATAACCTTGCTTTTGGGCTCATT
GAACGCGGTTTTTATAACGCGGGATCTTTTCAATGTATATGTTACCTTTGAACTTGCGTCGATAATAAGTTTCATATTGA
TAGCTTATGGTAGGAAAGGAAAACAGATCTGGGCTTCGTTGAAATATATGTTAATATCAGCCCTTGGCTTTAATTTCTAC
CTCATCGGAATAGGGCTCGTTTACATGCGAAACGGGACCTTCTCTTTTTCCGCTATGGATAACATGGATAAACTACCCGC
TATGCTTATATTTGTGGGCCTTTCCGTAAAATCGGGTTTGTTCTTGCTTTCAATGTGGCTTCCGGATGCGCATGCCAACG
CCATAACTGAGATTTCTCCCATACTTTCCGGATTAATGGTCAAAATAGAGGATTACCTTGTAATACGTTTTCTCCAATAC
AGTTCCTTTGAATGGCTCAGGGGAGTTTACCTCATAGTGGGATCCGCCAGTGCCGTTGCTGGTGTGATCTTTGCGATGAA
TGCCAGGAAGGCGAAGAATATTCTGGCTTACCATACATTTTCGCAGATCGGTTTCATAATTGCAGCGAGCAACATCGCCG
CGGCATGGCATGCTTTCAGTCACGCTGTTTTTAAGGGACTTCTTTTCATGACAGCAGGAAACATCTATGAGAGGCTTGGT
ACACAGAATATAAAAGAATGGAACGGGAAGGTACGCCTGAGGGAATATATCCCCCTTCTCGTGGGATCAGCGGCAATCGC
TGGTGCACCCTTAACATCCGGTTTCGTCACCAAGAACGGCATAATGGATTCCATCGTGGGGCCGGCAAAATATCTCCTGG
TGATAGCTTCTGTTGGAACGGTAATCAGCTTCGCTAAATTTGTCTTTATACGCACATCAAAAGAAAGAACTCAACTGCCA
TGGAATGTTATGCTCGCTTATGCCATCATGATTTTCACGATATTTGCTCATGGGATAATAGAATTTGACTCACATTATGT
TTTAGATGCCTTAGTGATGATCGCAGCTGGAATTCCGTTGTATTTCATGGTAAGAAAATCCTTCAGACCTTTACCCAAAA
CCTTCGAAAGGCTTGACAATGCTTTACTGATTTATCTCATATTCTTCGCGATAATGCTGACGTTTATTATTTGA

Upstream 100 bases:

>100_bases
CAATTTTGAGTTTATTGCTCGTTTTCACCATAATGCTTCACAACAGGTATCATACCGTTGATGTAAATCTGATAGAAAAG
ATTCTCGAAGGAGAGGAGAA

Downstream 100 bases:

>100_bases
GAGGGTAAAATCCGAGAGTCGAGAAACCGAGAAGGCTGCTACGCAGCGCTCCGAATCCCTTCGGGATTGCTACGACCGGC
TGCGCCGGTGACACGAACTC

Product: NADH/Ubiquinone/plastoquinone (complex I)

Products: NAD+; ubiquinol

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MILYISLIPLFLGGLSLILNRYRKITITLTFVSLISSALILILNIGNSYETVIGNWGTLGISIGYDEIALPFLLAVVVVL
FAVTLNSLHKDYDGIFYALITLLLGSLNAVFITRDLFNVYVTFELASIISFILIAYGRKGKQIWASLKYMLISALGFNFY
LIGIGLVYMRNGTFSFSAMDNMDKLPAMLIFVGLSVKSGLFLLSMWLPDAHANAITEISPILSGLMVKIEDYLVIRFLQY
SSFEWLRGVYLIVGSASAVAGVIFAMNARKAKNILAYHTFSQIGFIIAASNIAAAWHAFSHAVFKGLLFMTAGNIYERLG
TQNIKEWNGKVRLREYIPLLVGSAAIAGAPLTSGFVTKNGIMDSIVGPAKYLLVIASVGTVISFAKFVFIRTSKERTQLP
WNVMLAYAIMIFTIFAHGIIEFDSHYVLDALVMIAAGIPLYFMVRKSFRPLPKTFERLDNALLIYLIFFAIMLTFII

Sequences:

>Translated_477_residues
MILYISLIPLFLGGLSLILNRYRKITITLTFVSLISSALILILNIGNSYETVIGNWGTLGISIGYDEIALPFLLAVVVVL
FAVTLNSLHKDYDGIFYALITLLLGSLNAVFITRDLFNVYVTFELASIISFILIAYGRKGKQIWASLKYMLISALGFNFY
LIGIGLVYMRNGTFSFSAMDNMDKLPAMLIFVGLSVKSGLFLLSMWLPDAHANAITEISPILSGLMVKIEDYLVIRFLQY
SSFEWLRGVYLIVGSASAVAGVIFAMNARKAKNILAYHTFSQIGFIIAASNIAAAWHAFSHAVFKGLLFMTAGNIYERLG
TQNIKEWNGKVRLREYIPLLVGSAAIAGAPLTSGFVTKNGIMDSIVGPAKYLLVIASVGTVISFAKFVFIRTSKERTQLP
WNVMLAYAIMIFTIFAHGIIEFDSHYVLDALVMIAAGIPLYFMVRKSFRPLPKTFERLDNALLIYLIFFAIMLTFII
>Mature_477_residues
MILYISLIPLFLGGLSLILNRYRKITITLTFVSLISSALILILNIGNSYETVIGNWGTLGISIGYDEIALPFLLAVVVVL
FAVTLNSLHKDYDGIFYALITLLLGSLNAVFITRDLFNVYVTFELASIISFILIAYGRKGKQIWASLKYMLISALGFNFY
LIGIGLVYMRNGTFSFSAMDNMDKLPAMLIFVGLSVKSGLFLLSMWLPDAHANAITEISPILSGLMVKIEDYLVIRFLQY
SSFEWLRGVYLIVGSASAVAGVIFAMNARKAKNILAYHTFSQIGFIIAASNIAAAWHAFSHAVFKGLLFMTAGNIYERLG
TQNIKEWNGKVRLREYIPLLVGSAAIAGAPLTSGFVTKNGIMDSIVGPAKYLLVIASVGTVISFAKFVFIRTSKERTQLP
WNVMLAYAIMIFTIFAHGIIEFDSHYVLDALVMIAAGIPLYFMVRKSFRPLPKTFERLDNALLIYLIFFAIMLTFII

Specific function: Mnh complex is a Na(+)Li(+)/H(+) antiporter involved in Na(+) and/or Li(+) excretion and Na(+) resistance. Na(+)/H(+) antiport consumes a transmembrane electrical potential, and is thus inferred to be electrogenic. Does not transport K(+), Ca(2+) or Mg(2+

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=371, Percent_Identity=24.7978436657682, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788827, Length=304, Percent_Identity=26.3157894736842, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788831, Length=286, Percent_Identity=27.2727272727273, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788614, Length=310, Percent_Identity=29.3548387096774, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788829, Length=324, Percent_Identity=26.2345679012346, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788613, Length=320, Percent_Identity=26.25, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI145693160, Length=218, Percent_Identity=29.3577981651376, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI2367154, Length=422, Percent_Identity=24.1706161137441, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 52950; Mature: 52950

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILYISLIPLFLGGLSLILNRYRKITITLTFVSLISSALILILNIGNSYETVIGNWGTLG
CEEEEEHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHEEEEECCCCHHHHHCCCCEEE
ISIGYDEIALPFLLAVVVVLFAVTLNSLHKDYDGIFYALITLLLGSLNAVFITRDLFNVY
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHH
VTFELASIISFILIAYGRKGKQIWASLKYMLISALGFNFYLIGIGLVYMRNGTFSFSAMD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCEEEHHHC
NMDKLPAMLIFVGLSVKSGLFLLSMWLPDAHANAITEISPILSGLMVKIEDYLVIRFLQY
CHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSFEWLRGVYLIVGSASAVAGVIFAMNARKAKNILAYHTFSQIGFIIAASNIAAAWHAFS
CCHHHHCEEEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHH
HAVFKGLLFMTAGNIYERLGTQNIKEWNGKVRLREYIPLLVGSAAIAGAPLTSGFVTKNG
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEHHHHHHHHHCCHHHCCCCCCCCCEECCC
IMDSIVGPAKYLLVIASVGTVISFAKFVFIRTSKERTQLPWNVMLAYAIMIFTIFAHGII
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCHHHHHHHHHHHHHHHHHHHH
EFDSHYVLDALVMIAAGIPLYFMVRKSFRPLPKTFERLDNALLIYLIFFAIMLTFII
HCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MILYISLIPLFLGGLSLILNRYRKITITLTFVSLISSALILILNIGNSYETVIGNWGTLG
CEEEEEHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHEEEEECCCCHHHHHCCCCEEE
ISIGYDEIALPFLLAVVVVLFAVTLNSLHKDYDGIFYALITLLLGSLNAVFITRDLFNVY
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHH
VTFELASIISFILIAYGRKGKQIWASLKYMLISALGFNFYLIGIGLVYMRNGTFSFSAMD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCEEEHHHC
NMDKLPAMLIFVGLSVKSGLFLLSMWLPDAHANAITEISPILSGLMVKIEDYLVIRFLQY
CHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSFEWLRGVYLIVGSASAVAGVIFAMNARKAKNILAYHTFSQIGFIIAASNIAAAWHAFS
CCHHHHCEEEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHH
HAVFKGLLFMTAGNIYERLGTQNIKEWNGKVRLREYIPLLVGSAAIAGAPLTSGFVTKNG
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEHHHHHHHHHCCHHHCCCCCCCCCEECCC
IMDSIVGPAKYLLVIASVGTVISFAKFVFIRTSKERTQLPWNVMLAYAIMIFTIFAHGII
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCHHHHHHHHHHHHHHHHHHHH
EFDSHYVLDALVMIAAGIPLYFMVRKSFRPLPKTFERLDNALLIYLIFFAIMLTFII
HCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]