Click here to switch to the map view.

The map label for this gene is wecB [C]

Identifier: 239618003

GI number: 239618003

Start: 1750101

End: 1751174

Strand: Direct

Name: wecB [C]

Synonym: Kole_1631

Alternate gene names: 239618003

Gene position: 1750101-1751174 (Clockwise)

Preceding gene: 239618002

Following gene: 239618004

Centisome position: 76.02

GC content: 38.73

Gene sequence:

>1074_bases
GTGAAGGTTCTTTCAGTTATCGGAGCGCGTCCACAATTTATCAAAGAGGCAGTAGTGGGAGAGCAATTAAGGGCACTTGG
AATGCGTGAAATTCTTGTTCATACAGGTCAGCATTACGACGTAAATATGTCTGATGTTTTTTTCAAGTATCTCAAACTGA
AAAAGCCTGATTATTACCTTTCTGTGGGTTCTGGTACCCACGCATATCAAACCGGGACGACAATGATCAGGCTTGAAGAG
GTTGTGATTAAAGAAAAACCCGATGTATTGATTGTGTATGGAGATACTAACGCTACCATAGCTGGTGCTCTTGTAGGGGC
AAAACTAAAAATCTCTGTTGCTCATGTCGAAGCTGGTTTAAGGCAGGAACCCAAAGATATGCCTGAAGAAATAAATCGGG
TAGTTACTGATCATATCTCCAGATTTTTATTTTGCCCAACAAAAAGAGCGGTTGATAATCTCAAATCAGAAGGGATATCC
AAAGGTGTGTATTTTGTTGGAGACGTTATGTATGATCTTTTTCTGAAGGTAAAAGATAGTATAGACGTTCAAAAAGTTCT
CAAAAAGTATAGATTACAGAGAAAAAATTATATCCTTGCCACTGTCCATAGAGACTTCAACACAGATAACGAAGAACGAT
TAAGAAATATCCTAACGGCATTGGGAGAAATATCCCGTGAAATTCCTGTTGTTTTTCCCATGCATCCTAGAACGAAAAAG
GTTATAAATCAGAAAAATCTGTGGAATTTGTTGGGAAAGACCAGAGTAATAGATCCAGTACCTTATCACGAGCTTGTAGC
TCTGTTGACAAATTCTTTGAAGGTGATAACAGACAGCGGTGGATTACAGAAGGAAGCGTACTTCGCAGGTGTTTCGGCAA
TAGTGTTAATGCCGGATACAGGGTGGATTGAACTTGTAGAAGCTAATTGGAACTATCTGGCTGATACCCAAAAAGAGTTA
ATAGTTCAGAAAGCGCTGGAAAGTTCTTTAAATAAATGTCCTTTCGAACAATTTTATGGTCAAGGTGACGCAGGAAAGAA
AATAGCTGAGATAATATTGCGTCATCAGAAATAA

Upstream 100 bases:

>100_bases
GTCGAAAGCAAGACCATTATGTTTGGAATGAAAAGAAAGAAAGAGAATTTATCGATTTTATCATGAGAGTTATAGATTCC
TAAGAAAGGAAGAGATAACA

Downstream 100 bases:

>100_bases
AATCAGAGACTATTAACATCCTGCAATGATAAGAATGATCATAACAGTTTTTTTATAGAATTTATAAAAAGTACTAGACA
GTAAAATGGTAATGTTTCCA

Product: UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKVLSVIGARPQFIKEAVVGEQLRALGMREILVHTGQHYDVNMSDVFFKYLKLKKPDYYLSVGSGTHAYQTGTTMIRLEE
VVIKEKPDVLIVYGDTNATIAGALVGAKLKISVAHVEAGLRQEPKDMPEEINRVVTDHISRFLFCPTKRAVDNLKSEGIS
KGVYFVGDVMYDLFLKVKDSIDVQKVLKKYRLQRKNYILATVHRDFNTDNEERLRNILTALGEISREIPVVFPMHPRTKK
VINQKNLWNLLGKTRVIDPVPYHELVALLTNSLKVITDSGGLQKEAYFAGVSAIVLMPDTGWIELVEANWNYLADTQKEL
IVQKALESSLNKCPFEQFYGQGDAGKKIAEIILRHQK

Sequences:

>Translated_357_residues
MKVLSVIGARPQFIKEAVVGEQLRALGMREILVHTGQHYDVNMSDVFFKYLKLKKPDYYLSVGSGTHAYQTGTTMIRLEE
VVIKEKPDVLIVYGDTNATIAGALVGAKLKISVAHVEAGLRQEPKDMPEEINRVVTDHISRFLFCPTKRAVDNLKSEGIS
KGVYFVGDVMYDLFLKVKDSIDVQKVLKKYRLQRKNYILATVHRDFNTDNEERLRNILTALGEISREIPVVFPMHPRTKK
VINQKNLWNLLGKTRVIDPVPYHELVALLTNSLKVITDSGGLQKEAYFAGVSAIVLMPDTGWIELVEANWNYLADTQKEL
IVQKALESSLNKCPFEQFYGQGDAGKKIAEIILRHQK
>Mature_357_residues
MKVLSVIGARPQFIKEAVVGEQLRALGMREILVHTGQHYDVNMSDVFFKYLKLKKPDYYLSVGSGTHAYQTGTTMIRLEE
VVIKEKPDVLIVYGDTNATIAGALVGAKLKISVAHVEAGLRQEPKDMPEEINRVVTDHISRFLFCPTKRAVDNLKSEGIS
KGVYFVGDVMYDLFLKVKDSIDVQKVLKKYRLQRKNYILATVHRDFNTDNEERLRNILTALGEISREIPVVFPMHPRTKK
VINQKNLWNLLGKTRVIDPVPYHELVALLTNSLKVITDSGGLQKEAYFAGVSAIVLMPDTGWIELVEANWNYLADTQKEL
IVQKALESSLNKCPFEQFYGQGDAGKKIAEIILRHQK

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=381, Percent_Identity=30.9711286089239, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 40386; Mature: 40386

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLSVIGARPQFIKEAVVGEQLRALGMREILVHTGQHYDVNMSDVFFKYLKLKKPDYYL
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEE
SVGSGTHAYQTGTTMIRLEEVVIKEKPDVLIVYGDTNATIAGALVGAKLKISVAHVEAGL
EECCCCCEEECCCCCEEEHHHHHCCCCCEEEEECCCCCCHHHHEECCEEEEEEHHHHHCC
RQEPKDMPEEINRVVTDHISRFLFCPTKRAVDNLKSEGISKGVYFVGDVMYDLFLKVKDS
CCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
IDVQKVLKKYRLQRKNYILATVHRDFNTDNEERLRNILTALGEISREIPVVFPMHPRTKK
CCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCHHH
VINQKNLWNLLGKTRVIDPVPYHELVALLTNSLKVITDSGGLQKEAYFAGVSAIVLMPDT
HHCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHCCCEEEEEECCC
GWIELVEANWNYLADTQKELIVQKALESSLNKCPFEQFYGQGDAGKKIAEIILRHQK
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKVLSVIGARPQFIKEAVVGEQLRALGMREILVHTGQHYDVNMSDVFFKYLKLKKPDYYL
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEE
SVGSGTHAYQTGTTMIRLEEVVIKEKPDVLIVYGDTNATIAGALVGAKLKISVAHVEAGL
EECCCCCEEECCCCCEEEHHHHHCCCCCEEEEECCCCCCHHHHEECCEEEEEEHHHHHCC
RQEPKDMPEEINRVVTDHISRFLFCPTKRAVDNLKSEGISKGVYFVGDVMYDLFLKVKDS
CCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
IDVQKVLKKYRLQRKNYILATVHRDFNTDNEERLRNILTALGEISREIPVVFPMHPRTKK
CCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCHHH
VINQKNLWNLLGKTRVIDPVPYHELVALLTNSLKVITDSGGLQKEAYFAGVSAIVLMPDT
HHCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHCCCEEEEEECCC
GWIELVEANWNYLADTQKELIVQKALESSLNKCPFEQFYGQGDAGKKIAEIILRHQK
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA