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The map label for this gene is ligT [C]

Identifier: 239617935

GI number: 239617935

Start: 1674861

End: 1675487

Strand: Direct

Name: ligT [C]

Synonym: Kole_1563

Alternate gene names: 239617935

Gene position: 1674861-1675487 (Clockwise)

Preceding gene: 239617934

Following gene: 239617936

Centisome position: 72.75

GC content: 40.67

Gene sequence:

>627_bases
TTGCCAGTGGTGGTAGTTATTTCAGGATTTCTGGTAAGATCTTTGAAGGGGGGAAGTAAATTGAGGACCTTTATAGCCAT
AGATACTGGTAAAGGTGTTGCTTCGGTTGTTGATATGGTTGTCGATAAACTGAAAAGGATGGGTTTTAAAGCTTCATGGG
TACCGGGAAGCAACGCTCATTTAACGCTTTCATTTTTGGGTGAGATAGAGTTTAGTAAAGTTGAAATTCTTGCTTCTATG
CTTTCAAAGAGGTTAAGAGGTTTTCCTTCTTTCACTTTTTCTACGAAACAGCTCGGCTTTTTTAGACACAACAATCTGCC
AAGGGTAATATGGATAGGCGTGGAAAACACTCCTTTCTTGAACAATCTACATCGTGAAGTACGGGTGGCTCTTGGAAGTT
TGGGACTGGCAGCAGAAGACAAGTTTCATCCCCATATTACCGTTGGACGAATGAAGTACAGTCCCCCATACTGGAAAAAA
TTATTAGCGACGATCGAACTCGAGGAAATTGTGGTACCTGTAAATGGAGTTCACATCTATGAATCGGAACTTTTGAGCGA
AGGTGCTAATTACAGGAAAATCTTCAGTTGTAATTTTGAAGGAGGCCTGGTTAAACATGAATTCTAG

Upstream 100 bases:

>100_bases
CAGATTGTCCTAATTGGTTGCCTTTATCTGATTTTATTGACTGGAGTTGATTTTCAGTTATTGTTAGATGCTTTGCAATG
GATCGTTGGAGTGCTCACCG

Downstream 100 bases:

>100_bases
GGAAGACAAAGCGAAAAAAATCGCTCTTGAAAAAGCCATTAAGGAAATCGAAAAACAATTTGGTAAGGGAGCGGTAATGC
TCCTTGGTGATCAGGAAGCA

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MPVVVVISGFLVRSLKGGSKLRTFIAIDTGKGVASVVDMVVDKLKRMGFKASWVPGSNAHLTLSFLGEIEFSKVEILASM
LSKRLRGFPSFTFSTKQLGFFRHNNLPRVIWIGVENTPFLNNLHREVRVALGSLGLAAEDKFHPHITVGRMKYSPPYWKK
LLATIELEEIVVPVNGVHIYESELLSEGANYRKIFSCNFEGGLVKHEF

Sequences:

>Translated_208_residues
MPVVVVISGFLVRSLKGGSKLRTFIAIDTGKGVASVVDMVVDKLKRMGFKASWVPGSNAHLTLSFLGEIEFSKVEILASM
LSKRLRGFPSFTFSTKQLGFFRHNNLPRVIWIGVENTPFLNNLHREVRVALGSLGLAAEDKFHPHITVGRMKYSPPYWKK
LLATIELEEIVVPVNGVHIYESELLSEGANYRKIFSCNFEGGLVKHEF
>Mature_207_residues
PVVVVISGFLVRSLKGGSKLRTFIAIDTGKGVASVVDMVVDKLKRMGFKASWVPGSNAHLTLSFLGEIEFSKVEILASML
SKRLRGFPSFTFSTKQLGFFRHNNLPRVIWIGVENTPFLNNLHREVRVALGSLGLAAEDKFHPHITVGRMKYSPPYWKKL
LATIELEEIVVPVNGVHIYESELLSEGANYRKIFSCNFEGGLVKHEF

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 23263; Mature: 23131

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVVVISGFLVRSLKGGSKLRTFIAIDTGKGVASVVDMVVDKLKRMGFKASWVPGSNAH
CCEEEEEHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCE
LTLSFLGEIEFSKVEILASMLSKRLRGFPSFTFSTKQLGFFRHNNLPRVIWIGVENTPFL
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEECCCCCEEEEEECCCCCHH
NNLHREVRVALGSLGLAAEDKFHPHITVGRMKYSPPYWKKLLATIELEEIVVPVNGVHIY
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHEEHHHEEEECCCEEEE
ESELLSEGANYRKIFSCNFEGGLVKHEF
HHHHHHCCCCEEEEEEECCCCCEEECCC
>Mature Secondary Structure 
PVVVVISGFLVRSLKGGSKLRTFIAIDTGKGVASVVDMVVDKLKRMGFKASWVPGSNAH
CEEEEEHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCE
LTLSFLGEIEFSKVEILASMLSKRLRGFPSFTFSTKQLGFFRHNNLPRVIWIGVENTPFL
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEECCCCCEEEEEECCCCCHH
NNLHREVRVALGSLGLAAEDKFHPHITVGRMKYSPPYWKKLLATIELEEIVVPVNGVHIY
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHEEHHHEEEECCCEEEE
ESELLSEGANYRKIFSCNFEGGLVKHEF
HHHHHHCCCCEEEEEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]