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The map label for this gene is priA [H]

Identifier: 239617917

GI number: 239617917

Start: 1655157

End: 1657436

Strand: Direct

Name: priA [H]

Synonym: Kole_1545

Alternate gene names: 239617917

Gene position: 1655157-1657436 (Clockwise)

Preceding gene: 239617916

Following gene: 239617931

Centisome position: 71.9

GC content: 42.15

Gene sequence:

>2280_bases
GTGGCCATATCGGGTTCCCCTCTTCTCGATACTTTCACCTATCATACTGATTTCCCATTAACTCCGGGAGAAAGAGTTGT
TGTTGATTTCGCAAATAGAAGTGTTGTGGCTTACGTGGTCGAGATCACAGATGAGCTTCCGGAATTCGCCACAAAACCCG
TTCTTCAAAAACTCGATGAGAAGCCCTTTCTAACTTCTAAAGACGTAGAATTGGCATTGAGGATAAGTGAGCGGTTTCTT
TGTCCTGTTGGTAAGATCTTCGATCTTTTCTTTCCGCCAGGGAAGGTTATGAATGTAAAAAGTTTCGTCGTTCCGCAGTC
TCAGGAGCTTCCCATAAAGGACATAATACCGTTGACCGCGGCAAGGGAAAAATTTGGTGCTGTTACTATTGAAAACTGGA
AAAAATCAAGGAAAATAAGGATAGTACACAGTTACGAGCAAAAAAAGACAAGAGTCCAAAAGAAGAAGTTCGTTAGGTTG
CGTCTGTCTATTAAAGACTTAGAGAAAATAAAACTAACGGATCTTCAAAGGAAGGTCGTAGAGCATCTGTTTTCCTTTGA
TGTTATTGAGGTTCGCGAACTGATTAAGAATCTTGACTTAAAGAGCATCTCACCCATTCAAACGTTGGAGAAGAAAGGCA
TTTTAGAGGTCTTTGAAGATGAGACTTACGAATTCAGTGACTGGACAATAGCTCCGATAGATGAATTAACCTCCGAACAA
AGAAGGGTTTTAAATTCCATTGTGTACGATTTTAAAGGTGTTCATCTCATATATGGGCTTACGGGAACGGGAAAAACAGA
AGTTTATTTCAAAGCAATGGAAAAGGTGTTAACTGAAGGTGCTCAGGTGCTTTATCTCGTTCCTGAAGTATCTCTGACAC
CGCAGCTTTTGGCCAGAGTTAAGGGAACATTTCCCGGTCGGGAGGTAAGGGTATACCACAGTTATCTCAGTAAGGCTAAA
CGGCAGGGTATCTGGCTGGACGCTCACGAAGGTAACGTCGATATAATAATCGGTACGCGTAGCTCTATCTGGATACCTAT
GAGAAAGCTTGGACTGATAATTGTCGACGAGGAGCACGATGCCAGCTTCTATCAGCAGAGTTCGCCTCATTACGATGCCG
TTGAAGTGGCTGTGGAAAAGGGGAAGCTTCACAACATCCCTATTATACTCGGTTCTGCAACCCCGAGAGTTACCCATTAT
TTTAGGGCCATCGAAGGCGAATACAAGCTGCATAAGCTCAAGCACAGACCGGTTGGTAAATTACCATCACTTGAATTGAT
AGATATGAGAAAATCTAAAGGCTTTTATGTACTTTCTAAAAAAGCTCTGGAAGAGATAAAAGAACTTCTCAAAGATGAGA
AGCAGATTTTTGTTTTCGTTCATCGGAAAGGCTTTTCAAATTACGTGGTGTGTACCAATTGCGGAACGGTTATAAAATGC
CCAAATTGTGATATATCACTGACGTATCACCGCCACGGGAATATTCTCAAATGCCATTATTGTGGGTTTACTACCCCACC
TTTCTCAAACTGCCCCAATTGTGGAAGTGCGGCATTATCAGCACGTGGTTTCGGAACCGAAAGGGTAGAGTATGAACTTA
GGAATTTCTTTCCTTCGGTGAAGATTATAAGACTCGACAAAGAGACGATAGAGAGTCCTTCGGATTATGAGAAAGCGCTT
CGGATGATAGAACAGGAAGATGCTCAGGTGGTTGTTGGTACCAAGATGATCGCGAAGGGACTCGATTTTCCAAAGGTGGG
TTGTGTCATTGTAGTTGATTCAGACAGACTTATAAATCTTCCCAGGTACGATGCCACTGAAAACGCTTTTCAGTTGATTA
CACAGGCATCTGGAAGATCCGGCAGGGGTACCTTTGGGAAGACGCTCATACAATCTTTCAATCCAGATCATCCCGTCATA
CAAAACGCTGTAGAAAACGACTTTGAAAGGTTCTTTGAAACAGAAATAAAAACAAGGGAACTTTTGAAATATCCACCATT
CTCGTATTTTGTAGAAATGATAGTCGAGCATGTAGACGAGAAACGGTGCCAGAAAAAAGCGGAAGAAATCGCTGAACTTA
TTGGAAAAGAATTTTCTGAACAATTCTTCGAGATACATGGGCCTGTAGTACCGGTGATAAAGAAGTTCAAAGGACGCTTC
AGAATGAAGATATTCCTTAAACTGAAACCTGAGGCATCAATGAAGGAACTCAGGGAATTGATAAAAAAACATTCCGCCCA
GATCGACCTTGTCGTCGATGGTATAGGCGGAATGCTCTAG

Upstream 100 bases:

>100_bases
ATTTATTCTCTCTTTTTTGATGATGTTAAGGTTATAAAAGCCTCGAATGAAATGAAAAGAAGGATAATCTATATCAGGGG
TTGATATTATCTTTGTAAAA

Downstream 100 bases:

>100_bases
AGAAAACCAGACTTCTATTATCTTTTCAGTTCTCCAGGGAAGAAACAGGCGACCTTGTGGCCGTTTCCGACATCCTTCAA
TGGCGGTTCTTCCTTCGAAC

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 759; Mature: 758

Protein sequence:

>759_residues
MAISGSPLLDTFTYHTDFPLTPGERVVVDFANRSVVAYVVEITDELPEFATKPVLQKLDEKPFLTSKDVELALRISERFL
CPVGKIFDLFFPPGKVMNVKSFVVPQSQELPIKDIIPLTAAREKFGAVTIENWKKSRKIRIVHSYEQKKTRVQKKKFVRL
RLSIKDLEKIKLTDLQRKVVEHLFSFDVIEVRELIKNLDLKSISPIQTLEKKGILEVFEDETYEFSDWTIAPIDELTSEQ
RRVLNSIVYDFKGVHLIYGLTGTGKTEVYFKAMEKVLTEGAQVLYLVPEVSLTPQLLARVKGTFPGREVRVYHSYLSKAK
RQGIWLDAHEGNVDIIIGTRSSIWIPMRKLGLIIVDEEHDASFYQQSSPHYDAVEVAVEKGKLHNIPIILGSATPRVTHY
FRAIEGEYKLHKLKHRPVGKLPSLELIDMRKSKGFYVLSKKALEEIKELLKDEKQIFVFVHRKGFSNYVVCTNCGTVIKC
PNCDISLTYHRHGNILKCHYCGFTTPPFSNCPNCGSAALSARGFGTERVEYELRNFFPSVKIIRLDKETIESPSDYEKAL
RMIEQEDAQVVVGTKMIAKGLDFPKVGCVIVVDSDRLINLPRYDATENAFQLITQASGRSGRGTFGKTLIQSFNPDHPVI
QNAVENDFERFFETEIKTRELLKYPPFSYFVEMIVEHVDEKRCQKKAEEIAELIGKEFSEQFFEIHGPVVPVIKKFKGRF
RMKIFLKLKPEASMKELRELIKKHSAQIDLVVDGIGGML

Sequences:

>Translated_759_residues
MAISGSPLLDTFTYHTDFPLTPGERVVVDFANRSVVAYVVEITDELPEFATKPVLQKLDEKPFLTSKDVELALRISERFL
CPVGKIFDLFFPPGKVMNVKSFVVPQSQELPIKDIIPLTAAREKFGAVTIENWKKSRKIRIVHSYEQKKTRVQKKKFVRL
RLSIKDLEKIKLTDLQRKVVEHLFSFDVIEVRELIKNLDLKSISPIQTLEKKGILEVFEDETYEFSDWTIAPIDELTSEQ
RRVLNSIVYDFKGVHLIYGLTGTGKTEVYFKAMEKVLTEGAQVLYLVPEVSLTPQLLARVKGTFPGREVRVYHSYLSKAK
RQGIWLDAHEGNVDIIIGTRSSIWIPMRKLGLIIVDEEHDASFYQQSSPHYDAVEVAVEKGKLHNIPIILGSATPRVTHY
FRAIEGEYKLHKLKHRPVGKLPSLELIDMRKSKGFYVLSKKALEEIKELLKDEKQIFVFVHRKGFSNYVVCTNCGTVIKC
PNCDISLTYHRHGNILKCHYCGFTTPPFSNCPNCGSAALSARGFGTERVEYELRNFFPSVKIIRLDKETIESPSDYEKAL
RMIEQEDAQVVVGTKMIAKGLDFPKVGCVIVVDSDRLINLPRYDATENAFQLITQASGRSGRGTFGKTLIQSFNPDHPVI
QNAVENDFERFFETEIKTRELLKYPPFSYFVEMIVEHVDEKRCQKKAEEIAELIGKEFSEQFFEIHGPVVPVIKKFKGRF
RMKIFLKLKPEASMKELRELIKKHSAQIDLVVDGIGGML
>Mature_758_residues
AISGSPLLDTFTYHTDFPLTPGERVVVDFANRSVVAYVVEITDELPEFATKPVLQKLDEKPFLTSKDVELALRISERFLC
PVGKIFDLFFPPGKVMNVKSFVVPQSQELPIKDIIPLTAAREKFGAVTIENWKKSRKIRIVHSYEQKKTRVQKKKFVRLR
LSIKDLEKIKLTDLQRKVVEHLFSFDVIEVRELIKNLDLKSISPIQTLEKKGILEVFEDETYEFSDWTIAPIDELTSEQR
RVLNSIVYDFKGVHLIYGLTGTGKTEVYFKAMEKVLTEGAQVLYLVPEVSLTPQLLARVKGTFPGREVRVYHSYLSKAKR
QGIWLDAHEGNVDIIIGTRSSIWIPMRKLGLIIVDEEHDASFYQQSSPHYDAVEVAVEKGKLHNIPIILGSATPRVTHYF
RAIEGEYKLHKLKHRPVGKLPSLELIDMRKSKGFYVLSKKALEEIKELLKDEKQIFVFVHRKGFSNYVVCTNCGTVIKCP
NCDISLTYHRHGNILKCHYCGFTTPPFSNCPNCGSAALSARGFGTERVEYELRNFFPSVKIIRLDKETIESPSDYEKALR
MIEQEDAQVVVGTKMIAKGLDFPKVGCVIVVDSDRLINLPRYDATENAFQLITQASGRSGRGTFGKTLIQSFNPDHPVIQ
NAVENDFERFFETEIKTRELLKYPPFSYFVEMIVEHVDEKRCQKKAEEIAELIGKEFSEQFFEIHGPVVPVIKKFKGRFR
MKIFLKLKPEASMKELRELIKKHSAQIDLVVDGIGGML

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=762, Percent_Identity=30.7086614173228, Blast_Score=348, Evalue=9e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 86819; Mature: 86688

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISGSPLLDTFTYHTDFPLTPGERVVVDFANRSVVAYVVEITDELPEFATKPVLQKLDE
CCCCCCCCHHEEEECCCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHCCC
KPFLTSKDVELALRISERFLCPVGKIFDLFFPPGKVMNVKSFVVPQSQELPIKDIIPLTA
CCCCCCCCCEEEEEEHHHHCCCHHHHHHHHCCCCCEECCCEEECCCCCCCCHHHHCCHHH
AREKFGAVTIENWKKSRKIRIVHSYEQKKTRVQKKKFVRLRLSIKDLEKIKLTDLQRKVV
HHHHHCCEEECCCCCCCEEEEEECHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHHH
EHLFSFDVIEVRELIKNLDLKSISPIQTLEKKGILEVFEDETYEFSDWTIAPIDELTSEQ
HHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHCCCCEEECCEEECCHHHHHHHH
RRVLNSIVYDFKGVHLIYGLTGTGKTEVYFKAMEKVLTEGAQVLYLVPEVSLTPQLLARV
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
KGTFPGREVRVYHSYLSKAKRQGIWLDAHEGNVDIIIGTRSSIWIPMRKLGLIIVDEEHD
CCCCCCCEEHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCEEEEHHHCCEEEEECCCC
ASFYQQSSPHYDAVEVAVEKGKLHNIPIILGSATPRVTHYFRAIEGEYKLHKLKHRPVGK
CHHHHCCCCCCCHHHHEECCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHCCCCCCC
LPSLELIDMRKSKGFYVLSKKALEEIKELLKDEKQIFVFVHRKGFSNYVVCTNCGTVIKC
CCCCEEEEECCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCEEEC
PNCDISLTYHRHGNILKCHYCGFTTPPFSNCPNCGSAALSARGFGTERVEYELRNFFPSV
CCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCE
KIIRLDKETIESPSDYEKALRMIEQEDAQVVVGTKMIAKGLDFPKVGCVIVVDSDRLINL
EEEEECHHHCCCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCCEEEEECCCCEECC
PRYDATENAFQLITQASGRSGRGTFGKTLIQSFNPDHPVIQNAVENDFERFFETEIKTRE
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLKYPPFSYFVEMIVEHVDEKRCQKKAEEIAELIGKEFSEQFFEIHGPVVPVIKKFKGRF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCE
RMKIFLKLKPEASMKELRELIKKHSAQIDLVVDGIGGML
EEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCC
>Mature Secondary Structure 
AISGSPLLDTFTYHTDFPLTPGERVVVDFANRSVVAYVVEITDELPEFATKPVLQKLDE
CCCCCCCHHEEEECCCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHCCC
KPFLTSKDVELALRISERFLCPVGKIFDLFFPPGKVMNVKSFVVPQSQELPIKDIIPLTA
CCCCCCCCCEEEEEEHHHHCCCHHHHHHHHCCCCCEECCCEEECCCCCCCCHHHHCCHHH
AREKFGAVTIENWKKSRKIRIVHSYEQKKTRVQKKKFVRLRLSIKDLEKIKLTDLQRKVV
HHHHHCCEEECCCCCCCEEEEEECHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHHH
EHLFSFDVIEVRELIKNLDLKSISPIQTLEKKGILEVFEDETYEFSDWTIAPIDELTSEQ
HHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHCCCCEEECCEEECCHHHHHHHH
RRVLNSIVYDFKGVHLIYGLTGTGKTEVYFKAMEKVLTEGAQVLYLVPEVSLTPQLLARV
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
KGTFPGREVRVYHSYLSKAKRQGIWLDAHEGNVDIIIGTRSSIWIPMRKLGLIIVDEEHD
CCCCCCCEEHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCEEEEHHHCCEEEEECCCC
ASFYQQSSPHYDAVEVAVEKGKLHNIPIILGSATPRVTHYFRAIEGEYKLHKLKHRPVGK
CHHHHCCCCCCCHHHHEECCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHCCCCCCC
LPSLELIDMRKSKGFYVLSKKALEEIKELLKDEKQIFVFVHRKGFSNYVVCTNCGTVIKC
CCCCEEEEECCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCEEEC
PNCDISLTYHRHGNILKCHYCGFTTPPFSNCPNCGSAALSARGFGTERVEYELRNFFPSV
CCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCE
KIIRLDKETIESPSDYEKALRMIEQEDAQVVVGTKMIAKGLDFPKVGCVIVVDSDRLINL
EEEEECHHHCCCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCCEEEEECCCCEECC
PRYDATENAFQLITQASGRSGRGTFGKTLIQSFNPDHPVIQNAVENDFERFFETEIKTRE
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLKYPPFSYFVEMIVEHVDEKRCQKKAEEIAELIGKEFSEQFFEIHGPVVPVIKKFKGRF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCE
RMKIFLKLKPEASMKELRELIKKHSAQIDLVVDGIGGML
EEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]