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The map label for this gene is infB

Identifier: 239617778

GI number: 239617778

Start: 1495005

End: 1497092

Strand: Reverse

Name: infB

Synonym: Kole_1403

Alternate gene names: 239617778

Gene position: 1497092-1495005 (Counterclockwise)

Preceding gene: 239617779

Following gene: 239617776

Centisome position: 65.03

GC content: 42.62

Gene sequence:

>2088_bases
ATGGCGAAGACCCGAGTTTATGAACTCGCTAAAAGATTGAAAATCACTACCAGGGAACTTATAGACGAATTGGAGGAGCT
GGGAGTCTCTGTTAAAAGTCACATGTCCGTTTTAGATGATGAAATTGTCAATATAATCGTCGGCCTGTATGAGGAAGAAG
AAAAAGCAGCAAAAATTAAGAAGTCAGCTTCTTCCAAGAAGAAAACCGAAAAAGAAGTAGAGGAAGAAGAAATTGAAACT
CCCAAAAAGAAAAAGAAACAGGAGGAGAAAATCCCTGGTGAGGAAAAAGTCCTTCGTATCACCCCCGATGAATTGAAGCT
TGACTTACTCGCCGAGAAAATGAGGGTACCTGTTTCAAAAATCGTGAAAGATCATTTTATGAAAGGGATCATATTACGCC
CCGCACAGTCATTATCACTTGAAGACGCTAATCAAATTGCCGCTCAATACGGTTGGAAGCTTGAAATAGAAAAAGAAGAG
ATGGCTGACCCGCTGGAAGCACTCAAGAAGAAATATGAAGAACTATACAAAGATGAATCCAGACTTGTACAACGTCCCCC
GGTAGTAACGGTTATGGGTCATGTTGACCATGGAAAAACAACGCTTCTCGATAGAATAAGAAAAACCAGTATAGCAGAAA
AAGAAGTTGGTGGCATCACACAATCAATCGGTGCGTACCATGTAGAGGTAAACGGAAAAAAAATAACCTTTATAGACACT
CCCGGCCACGAAGCATTCACAGAAATGAGAGCAAGAGGTGCTCAAGCAACGGATATAGTCATCCTTGTGGTTGCGGCCGA
TGATGGTGTCATGCCTCAAACCGTTGAAGCTTACAACCACGCAAAAACCGCTCAGGTTCCTATAATAGTCGCCATAAATA
AAATCGACAAACCAAATGCAAGTATCGAAGCAACCAAACAGCAGCTTGCTTCAAAACTCGGGCTCGTTCCCGAAGATTGG
GGCGGAGATACGATAGTTGTTCCCATATCGGCAAAAACAGGCCAGGGTATCGACGAACTTCTGGAAATGATTCTGCTTGT
GGCCGAGATGTCGGAAATAAAGTGTATTCCCACAGGAAATGCTAGAGGAATTATTATCGAGTCTGAGCTTGATAAAGGTG
TTGGTCCACTTGCGACAGTTATAGTTAAGGATGGGATATTGGAAGCCGGCGACTATATCGTTGCAGGAGCTACATACGGT
AAAGTTCGCGCTTTGAGAGATGAAAAGGGTAAGCGTGTTAAAAAAGCTGTCCCGGGCGACCCTGTTCAGATAATAGGGTT
CAATGAGGTACCGGATGTTCACGCCATACTCTACGTGGTAGATTCTCTCGATCAAGCGAGAGAAGTAGCGGCCTTTGCTC
AGGAAAAACAAAAGAAAGAAAAGCTACTGAAAGGCAAGCGTCACGTGAGATTGGAAGAATTCATGCGTATCGGTGGTAAA
GACGAAACAAAAGTCCTGAACCTCATACTTAAGTCCGACTCCTTTGGTTCTGTCGAAGCTTTGAGACAAACGATAGCCAA
ACTGGAAACAGAAGAAGTTCATATCGAGGTAGTTCATTTTGGAATAGGTACGATAAACGCAAGTGACGTTATGCTTGCAG
CAGCTTCTGATGCCGTAATTATAGGTTACAAAGTAAAGCCAGACAGCCAGGCTCGAAGACAAGCAGAAGAGGAAGGCGTT
CAGATCCGCGTTTATCAGGTAATCTTCGATCTCATCGATGACCTCAAAAAAGCCCTTGAAGGTCTTCTGGAACCCGAAGA
AATTGATGAAACCGTTGGCCATGGCGAAATCAGAAAAGTGTTTAAGATAAAGAAAGTTGGTAGCATAGCCGGTGTACAAT
TACTCGATGGCTATGTAACAAAAAAAGGTTTCGTCAGAATATACAGGAACAATCAAGAGATATTCGATGGTGAAATTGAG
TCGCTTAAACATTACAAAGATGAAGTCTCCAGAATAGACGCACCCAAAGAATGCGGAATCAAATTCCTGAACTTTGACGA
TATTCAAGAAGGTGATCAGTTAGAGTTCCATGTAAAGAGGAAAGTGAAGAGAACATTAGACTTCAACGAAAGCTCTTCGG
ATAGCTAA

Upstream 100 bases:

>100_bases
GCAAAAGCGTTAGGCAAAGTCAGCTTTTCAGCCGTTGGAGTTACCGATGAAGAACTCGCAGCTGAGATCGTAAGATTAGC
CGAAGCAGGAGGTGAAAAAA

Downstream 100 bases:

>100_bases
ATAACGAGAACCGAGAGTCCGAGAGCCGAGAGCCTAGAAATAGAAAATGGGGACTGTCCAACAGAGGACTGTCCCCTTTT
TCCCTAATCTCAGGTTTTTC

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MAKTRVYELAKRLKITTRELIDELEELGVSVKSHMSVLDDEIVNIIVGLYEEEEKAAKIKKSASSKKKTEKEVEEEEIET
PKKKKKQEEKIPGEEKVLRITPDELKLDLLAEKMRVPVSKIVKDHFMKGIILRPAQSLSLEDANQIAAQYGWKLEIEKEE
MADPLEALKKKYEELYKDESRLVQRPPVVTVMGHVDHGKTTLLDRIRKTSIAEKEVGGITQSIGAYHVEVNGKKITFIDT
PGHEAFTEMRARGAQATDIVILVVAADDGVMPQTVEAYNHAKTAQVPIIVAINKIDKPNASIEATKQQLASKLGLVPEDW
GGDTIVVPISAKTGQGIDELLEMILLVAEMSEIKCIPTGNARGIIIESELDKGVGPLATVIVKDGILEAGDYIVAGATYG
KVRALRDEKGKRVKKAVPGDPVQIIGFNEVPDVHAILYVVDSLDQAREVAAFAQEKQKKEKLLKGKRHVRLEEFMRIGGK
DETKVLNLILKSDSFGSVEALRQTIAKLETEEVHIEVVHFGIGTINASDVMLAAASDAVIIGYKVKPDSQARRQAEEEGV
QIRVYQVIFDLIDDLKKALEGLLEPEEIDETVGHGEIRKVFKIKKVGSIAGVQLLDGYVTKKGFVRIYRNNQEIFDGEIE
SLKHYKDEVSRIDAPKECGIKFLNFDDIQEGDQLEFHVKRKVKRTLDFNESSSDS

Sequences:

>Translated_695_residues
MAKTRVYELAKRLKITTRELIDELEELGVSVKSHMSVLDDEIVNIIVGLYEEEEKAAKIKKSASSKKKTEKEVEEEEIET
PKKKKKQEEKIPGEEKVLRITPDELKLDLLAEKMRVPVSKIVKDHFMKGIILRPAQSLSLEDANQIAAQYGWKLEIEKEE
MADPLEALKKKYEELYKDESRLVQRPPVVTVMGHVDHGKTTLLDRIRKTSIAEKEVGGITQSIGAYHVEVNGKKITFIDT
PGHEAFTEMRARGAQATDIVILVVAADDGVMPQTVEAYNHAKTAQVPIIVAINKIDKPNASIEATKQQLASKLGLVPEDW
GGDTIVVPISAKTGQGIDELLEMILLVAEMSEIKCIPTGNARGIIIESELDKGVGPLATVIVKDGILEAGDYIVAGATYG
KVRALRDEKGKRVKKAVPGDPVQIIGFNEVPDVHAILYVVDSLDQAREVAAFAQEKQKKEKLLKGKRHVRLEEFMRIGGK
DETKVLNLILKSDSFGSVEALRQTIAKLETEEVHIEVVHFGIGTINASDVMLAAASDAVIIGYKVKPDSQARRQAEEEGV
QIRVYQVIFDLIDDLKKALEGLLEPEEIDETVGHGEIRKVFKIKKVGSIAGVQLLDGYVTKKGFVRIYRNNQEIFDGEIE
SLKHYKDEVSRIDAPKECGIKFLNFDDIQEGDQLEFHVKRKVKRTLDFNESSSDS
>Mature_694_residues
AKTRVYELAKRLKITTRELIDELEELGVSVKSHMSVLDDEIVNIIVGLYEEEEKAAKIKKSASSKKKTEKEVEEEEIETP
KKKKKQEEKIPGEEKVLRITPDELKLDLLAEKMRVPVSKIVKDHFMKGIILRPAQSLSLEDANQIAAQYGWKLEIEKEEM
ADPLEALKKKYEELYKDESRLVQRPPVVTVMGHVDHGKTTLLDRIRKTSIAEKEVGGITQSIGAYHVEVNGKKITFIDTP
GHEAFTEMRARGAQATDIVILVVAADDGVMPQTVEAYNHAKTAQVPIIVAINKIDKPNASIEATKQQLASKLGLVPEDWG
GDTIVVPISAKTGQGIDELLEMILLVAEMSEIKCIPTGNARGIIIESELDKGVGPLATVIVKDGILEAGDYIVAGATYGK
VRALRDEKGKRVKKAVPGDPVQIIGFNEVPDVHAILYVVDSLDQAREVAAFAQEKQKKEKLLKGKRHVRLEEFMRIGGKD
ETKVLNLILKSDSFGSVEALRQTIAKLETEEVHIEVVHFGIGTINASDVMLAAASDAVIIGYKVKPDSQARRQAEEEGVQ
IRVYQVIFDLIDDLKKALEGLLEPEEIDETVGHGEIRKVFKIKKVGSIAGVQLLDGYVTKKGFVRIYRNNQEIFDGEIES
LKHYKDEVSRIDAPKECGIKFLNFDDIQEGDQLEFHVKRKVKRTLDFNESSSDS

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI53729339, Length=546, Percent_Identity=41.025641025641, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI53729337, Length=546, Percent_Identity=41.025641025641, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI84043963, Length=466, Percent_Identity=28.9699570815451, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI157426893, Length=228, Percent_Identity=31.140350877193, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1789559, Length=577, Percent_Identity=45.9272097053726, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1788922, Length=237, Percent_Identity=30.379746835443, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI2367247, Length=249, Percent_Identity=30.9236947791165, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI48994988, Length=129, Percent_Identity=36.4341085271318, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790412, Length=127, Percent_Identity=37.7952755905512, Blast_Score=66, Evalue=9e-12,
Organism=Escherichia coli, GI1789737, Length=127, Percent_Identity=37.7952755905512, Blast_Score=66, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71994658, Length=549, Percent_Identity=33.3333333333333, Blast_Score=301, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI212656558, Length=563, Percent_Identity=26.9982238010657, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=217, Percent_Identity=32.7188940092166, Blast_Score=100, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17556456, Length=134, Percent_Identity=38.8059701492537, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17556745, Length=151, Percent_Identity=31.7880794701987, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324550, Length=622, Percent_Identity=35.2090032154341, Blast_Score=323, Evalue=8e-89,
Organism=Saccharomyces cerevisiae, GI6319282, Length=276, Percent_Identity=32.2463768115942, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=234, Percent_Identity=29.9145299145299, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=125, Percent_Identity=36.8, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28572034, Length=542, Percent_Identity=39.8523985239852, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24656849, Length=464, Percent_Identity=27.5862068965517, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI160714833, Length=73, Percent_Identity=67.1232876712329, Blast_Score=103, Evalue=5e-22,
Organism=Drosophila melanogaster, GI78706572, Length=235, Percent_Identity=31.4893617021277, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19922690, Length=175, Percent_Identity=33.1428571428571, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI281363316, Length=157, Percent_Identity=33.1210191082803, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17864358, Length=157, Percent_Identity=33.1210191082803, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2_KOSOT (C5CDZ4)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941100.1
- GeneID:   7968717
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1403
- ProtClustDB:   PRK05306
- GO:   GO:0005737
- HAMAP:   MF_00100_B
- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- TIGRFAMs:   TIGR00487
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 77428; Mature: 77297

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTRVYELAKRLKITTRELIDELEELGVSVKSHMSVLDDEIVNIIVGLYEEEEKAAKIK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KSASSKKKTEKEVEEEEIETPKKKKKQEEKIPGEEKVLRITPDELKLDLLAEKMRVPVSK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCCHHH
IVKDHFMKGIILRPAQSLSLEDANQIAAQYGWKLEIEKEEMADPLEALKKKYEELYKDES
HHHHHHHCCEEECCCCCCCCCHHHHHHHHCCCEEEECHHHHCCHHHHHHHHHHHHHCCHH
RLVQRPPVVTVMGHVDHGKTTLLDRIRKTSIAEKEVGGITQSIGAYHVEVNGKKITFIDT
HHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEEECCCEEEEEEC
PGHEAFTEMRARGAQATDIVILVVAADDGVMPQTVEAYNHAKTAQVPIIVAINKIDKPNA
CCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCCCC
SIEATKQQLASKLGLVPEDWGGDTIVVPISAKTGQGIDELLEMILLVAEMSEIKCIPTGN
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCC
ARGIIIESELDKGVGPLATVIVKDGILEAGDYIVAGATYGKVRALRDEKGKRVKKAVPGD
CCEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHCCHHHHCCCCC
PVQIIGFNEVPDVHAILYVVDSLDQAREVAAFAQEKQKKEKLLKGKRHVRLEEFMRIGGK
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHCCCC
DETKVLNLILKSDSFGSVEALRQTIAKLETEEVHIEVVHFGIGTINASDVMLAAASDAVI
CHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEECCCEEE
IGYKVKPDSQARRQAEEEGVQIRVYQVIFDLIDDLKKALEGLLEPEEIDETVGHGEIRKV
EEEEECCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHH
FKIKKVGSIAGVQLLDGYVTKKGFVRIYRNNQEIFDGEIESLKHYKDEVSRIDAPKECGI
HHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHCCCCHHHCE
KFLNFDDIQEGDQLEFHVKRKVKRTLDFNESSSDS
EEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AKTRVYELAKRLKITTRELIDELEELGVSVKSHMSVLDDEIVNIIVGLYEEEEKAAKIK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KSASSKKKTEKEVEEEEIETPKKKKKQEEKIPGEEKVLRITPDELKLDLLAEKMRVPVSK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCCHHH
IVKDHFMKGIILRPAQSLSLEDANQIAAQYGWKLEIEKEEMADPLEALKKKYEELYKDES
HHHHHHHCCEEECCCCCCCCCHHHHHHHHCCCEEEECHHHHCCHHHHHHHHHHHHHCCHH
RLVQRPPVVTVMGHVDHGKTTLLDRIRKTSIAEKEVGGITQSIGAYHVEVNGKKITFIDT
HHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEEECCCEEEEEEC
PGHEAFTEMRARGAQATDIVILVVAADDGVMPQTVEAYNHAKTAQVPIIVAINKIDKPNA
CCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCCCC
SIEATKQQLASKLGLVPEDWGGDTIVVPISAKTGQGIDELLEMILLVAEMSEIKCIPTGN
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCC
ARGIIIESELDKGVGPLATVIVKDGILEAGDYIVAGATYGKVRALRDEKGKRVKKAVPGD
CCEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHCCHHHHCCCCC
PVQIIGFNEVPDVHAILYVVDSLDQAREVAAFAQEKQKKEKLLKGKRHVRLEEFMRIGGK
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHCCCC
DETKVLNLILKSDSFGSVEALRQTIAKLETEEVHIEVVHFGIGTINASDVMLAAASDAVI
CHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEECCCEEE
IGYKVKPDSQARRQAEEEGVQIRVYQVIFDLIDDLKKALEGLLEPEEIDETVGHGEIRKV
EEEEECCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHH
FKIKKVGSIAGVQLLDGYVTKKGFVRIYRNNQEIFDGEIESLKHYKDEVSRIDAPKECGI
HHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHCCCCHHHCE
KFLNFDDIQEGDQLEFHVKRKVKRTLDFNESSSDS
EEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA