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The map label for this gene is gdhA [H]

Identifier: 239617777

GI number: 239617777

Start: 1493463

End: 1494716

Strand: Direct

Name: gdhA [H]

Synonym: Kole_1402

Alternate gene names: 239617777

Gene position: 1493463-1494716 (Clockwise)

Preceding gene: 239617773

Following gene: 239617785

Centisome position: 64.87

GC content: 44.9

Gene sequence:

>1254_bases
GTGTCCGACATCAGTCTTTTTGAAAATGCTTTAAAGCAATTTCGCAAGGCTGCGGAAGTTATGGAACTCGATCCCTGTAT
AGCCGAGGTTCTTTCGCACCCTAAAAGGGAGCTTACAGTCCATTTCCCAGTAAGAATGGATGATGGAAGCATTAAAGTAT
TCACCGGACACAGGGTCCAGCATAACATTGCAAGAGGACCAGCTAAAGGTGGTATCCGTTATCACCCAAGCGTTACTCTT
GATGAAGTCAAAGCTCTCGCTTTCTGGATGACATGGAAATGTGCCGTTGTTGGGATTCCCTATGGCGGTGGTAAGGGTGG
AGTTGCTGTTGATCCAGCAGAACTTTCACCAGCTGAATTAGAAAGACTCTCTCGAAGGTTCTTCTCCGAAATTCAGGTAA
TAATCGGTGAGGATAAAGATATACCTGCTCCAGATGTTAATACGAACCCCCAGGTCATGGCATGGTATATGGATACGTAT
TCCATGAATGTTGGCCATTCGGTTTTAGGAATCGTTACCGGTAAGCCGTTAGATGTTGGCGGTTCTGCTGGTAGAACAGA
AGCAACTGGTAGAGGTGTCAGGGTAGTCACTGAAGAGGCTATAAACTACAACGGCCTTGATCCCAAGAACTGTACAGTTG
CCGTTCAGGGATTCGGAAATGTTGGTTCTTACGCCGCAAAGCTCATAAAGGAAGAAGTTGGAAGCAAAATCATTGCTGTT
AGCGATGTTTCTGGTGCTATTTATAATCCGGATGGCCTGGATATAGATGATGTTGTAGCCTACCGCGATCAAAACAATGG
ACTCATTAAAGGATATCCAAAGGCAACTGCAATGACAAACGAGGAACTGCTGACAATGGACGTTGACATCCTTATTCCCG
CTGCTCTCGAAAACGCGATCACAATGAACAACGTTGAAGACGTTAAGGCAAAAATAATTGTTGAAGGTGCAAACGGACCT
GTAACCCCAGAAGCTGAAGAGGTTCTCCTTAAAAAAGGTGTCTTCATAGTTCCGGACTTCCTGGCCAACGCTGGAGGTGT
TACTGTTTCATATTTCGAATGGGTACAGGGTCTTCAGTGGTACTTCTGGGACATCGAAGATGTTAGGAAGGCACTTCATA
AAATAATGCGCGATTCCTTCTACAGTGTTATCAACACGATGAGAAAGTATGACACAGATATGAGAACGGCAGCTTACATC
GTTGCCATCGATAGGGTAGCAACTGCGACGAAGCTTAGAGGTATTTATCCATAA

Upstream 100 bases:

>100_bases
AAATAACGCCTTTCCTTTATGATTTTGTGGAGTTGTTCACAAATAAAGGCGAGGAATAAAATCAACGTGGAAAACCACTT
TTTCTTGAGGAGGGTTACAA

Downstream 100 bases:

>100_bases
GAAAAACGAGAATCCGAGAAAACTGCTTCGCAGCGAAGATACGAATCCCTTCGGGATTGTTGCGAACTCCTTCGGAGTTG
TTTCGAACCATTTCGTGGCT

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSDISLFENALKQFRKAAEVMELDPCIAEVLSHPKRELTVHFPVRMDDGSIKVFTGHRVQHNIARGPAKGGIRYHPSVTL
DEVKALAFWMTWKCAVVGIPYGGGKGGVAVDPAELSPAELERLSRRFFSEIQVIIGEDKDIPAPDVNTNPQVMAWYMDTY
SMNVGHSVLGIVTGKPLDVGGSAGRTEATGRGVRVVTEEAINYNGLDPKNCTVAVQGFGNVGSYAAKLIKEEVGSKIIAV
SDVSGAIYNPDGLDIDDVVAYRDQNNGLIKGYPKATAMTNEELLTMDVDILIPAALENAITMNNVEDVKAKIIVEGANGP
VTPEAEEVLLKKGVFIVPDFLANAGGVTVSYFEWVQGLQWYFWDIEDVRKALHKIMRDSFYSVINTMRKYDTDMRTAAYI
VAIDRVATATKLRGIYP

Sequences:

>Translated_417_residues
MSDISLFENALKQFRKAAEVMELDPCIAEVLSHPKRELTVHFPVRMDDGSIKVFTGHRVQHNIARGPAKGGIRYHPSVTL
DEVKALAFWMTWKCAVVGIPYGGGKGGVAVDPAELSPAELERLSRRFFSEIQVIIGEDKDIPAPDVNTNPQVMAWYMDTY
SMNVGHSVLGIVTGKPLDVGGSAGRTEATGRGVRVVTEEAINYNGLDPKNCTVAVQGFGNVGSYAAKLIKEEVGSKIIAV
SDVSGAIYNPDGLDIDDVVAYRDQNNGLIKGYPKATAMTNEELLTMDVDILIPAALENAITMNNVEDVKAKIIVEGANGP
VTPEAEEVLLKKGVFIVPDFLANAGGVTVSYFEWVQGLQWYFWDIEDVRKALHKIMRDSFYSVINTMRKYDTDMRTAAYI
VAIDRVATATKLRGIYP
>Mature_416_residues
SDISLFENALKQFRKAAEVMELDPCIAEVLSHPKRELTVHFPVRMDDGSIKVFTGHRVQHNIARGPAKGGIRYHPSVTLD
EVKALAFWMTWKCAVVGIPYGGGKGGVAVDPAELSPAELERLSRRFFSEIQVIIGEDKDIPAPDVNTNPQVMAWYMDTYS
MNVGHSVLGIVTGKPLDVGGSAGRTEATGRGVRVVTEEAINYNGLDPKNCTVAVQGFGNVGSYAAKLIKEEVGSKIIAVS
DVSGAIYNPDGLDIDDVVAYRDQNNGLIKGYPKATAMTNEELLTMDVDILIPAALENAITMNNVEDVKAKIIVEGANGPV
TPEAEEVLLKKGVFIVPDFLANAGGVTVSYFEWVQGLQWYFWDIEDVRKALHKIMRDSFYSVINTMRKYDTDMRTAAYIV
AIDRVATATKLRGIYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=434, Percent_Identity=40.5529953917051, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI4885281, Length=365, Percent_Identity=44.3835616438356, Blast_Score=295, Evalue=5e-80,
Organism=Escherichia coli, GI1788059, Length=402, Percent_Identity=32.5870646766169, Blast_Score=185, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17544676, Length=442, Percent_Identity=37.3303167420815, Blast_Score=264, Evalue=8e-71,
Organism=Saccharomyces cerevisiae, GI6319256, Length=396, Percent_Identity=33.8383838383838, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6324951, Length=441, Percent_Identity=31.0657596371882, Blast_Score=162, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24649283, Length=389, Percent_Identity=41.9023136246787, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI45553475, Length=461, Percent_Identity=35.1409978308026, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI45549226, Length=383, Percent_Identity=38.9033942558747, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24649460, Length=313, Percent_Identity=39.297124600639, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 45646; Mature: 45515

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDISLFENALKQFRKAAEVMELDPCIAEVLSHPKRELTVHFPVRMDDGSIKVFTGHRVQ
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEEEEEECCCCEEEEECCCHH
HNIARGPAKGGIRYHPSVTLDEVKALAFWMTWKCAVVGIPYGGGKGGVAVDPAELSPAEL
HHHHCCCCCCCEEECCCCCHHHHHHHHHHEEEEEEEEEECCCCCCCCCEECCCCCCHHHH
ERLSRRFFSEIQVIIGEDKDIPAPDVNTNPQVMAWYMDTYSMNVGHSVLGIVTGKPLDVG
HHHHHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCC
GSAGRTEATGRGVRVVTEEAINYNGLDPKNCTVAVQGFGNVGSYAAKLIKEEVGSKIIAV
CCCCCCCCCCCCEEEEEEHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEE
SDVSGAIYNPDGLDIDDVVAYRDQNNGLIKGYPKATAMTNEELLTMDVDILIPAALENAI
EECCCCEECCCCCCHHHHEEEEECCCCEEECCCCCCCCCCCCEEEEEEHEEEHHHHCCCE
TMNNVEDVKAKIIVEGANGPVTPEAEEVLLKKGVFIVPDFLANAGGVTVSYFEWVQGLQW
ECCCHHCCEEEEEEECCCCCCCCCHHHHHHHCCCEEEEHHHCCCCCCEEHHHHHHCCCCE
YFWDIEDVRKALHKIMRDSFYSVINTMRKYDTDMRTAAYIVAIDRVATATKLRGIYP
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDISLFENALKQFRKAAEVMELDPCIAEVLSHPKRELTVHFPVRMDDGSIKVFTGHRVQ
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEEEEEECCCCEEEEECCCHH
HNIARGPAKGGIRYHPSVTLDEVKALAFWMTWKCAVVGIPYGGGKGGVAVDPAELSPAEL
HHHHCCCCCCCEEECCCCCHHHHHHHHHHEEEEEEEEEECCCCCCCCCEECCCCCCHHHH
ERLSRRFFSEIQVIIGEDKDIPAPDVNTNPQVMAWYMDTYSMNVGHSVLGIVTGKPLDVG
HHHHHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCC
GSAGRTEATGRGVRVVTEEAINYNGLDPKNCTVAVQGFGNVGSYAAKLIKEEVGSKIIAV
CCCCCCCCCCCCEEEEEEHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEE
SDVSGAIYNPDGLDIDDVVAYRDQNNGLIKGYPKATAMTNEELLTMDVDILIPAALENAI
EECCCCEECCCCCCHHHHEEEEECCCCEEECCCCCCCCCCCCEEEEEEHEEEHHHHCCCE
TMNNVEDVKAKIIVEGANGPVTPEAEEVLLKKGVFIVPDFLANAGGVTVSYFEWVQGLQW
ECCCHHCCEEEEEEECCCCCCCCCHHHHHHHCCCEEEEHHHCCCCCCEEHHHHHHCCCCE
YFWDIEDVRKALHKIMRDSFYSVINTMRKYDTDMRTAAYIVAIDRVATATKLRGIYP
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]