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The map label for this gene is psuG

Identifier: 239617775

GI number: 239617775

Start: 1491474

End: 1492343

Strand: Reverse

Name: psuG

Synonym: Kole_1400

Alternate gene names: 239617775

Gene position: 1492343-1491474 (Counterclockwise)

Preceding gene: 239617776

Following gene: 239617774

Centisome position: 64.82

GC content: 43.68

Gene sequence:

>870_bases
ATGAAGAAACAACCCATTGTAGCTCTAGAATCAACGGTAATAGCTCATGGCTTACCATGGCCAACTAATTTTGAAACAGC
GGTTTCCATGGAAAACAAAGTAAAAGAAAAAGGTGCCATACCAAAGACATTGGGCATAATAAACGGCGAGATAAAGATAG
GCTTGACAGAAAAAGAAATAGAACATCTGGCAAAAGCAAAGAATGTTATGAAGATCGGTACGGCTGAAATCGCGGCAGCC
ATTGCCTTAAGGAGAAATGCAGCAACCACCGTCAGTGCCACGATGCGCCTGGCGAAAAACGCAGGGATAGACGTTTTTGC
TACCGGTGGCATAGGCGGGGTACATCGGAACGTTCCCTGGGATGTTTCACAGGACATAATGGAACTCTCCAAAACACGAA
TGATTGTGGTTTGTGCCGGTTTCAAGAGCATTTTAGACGTAGAAAGAACTATAGAATTTCTCGAAACCTTTCAGGTGACC
GTTGTCGGATATCAAACGGACTATATGCCCTTATTCCACACAAAAGAAAGCGAATACAAAGTCAACATACGTGCTGATTC
CCCCGAAGAGATCGTGGCTATATTCAACGAAAAAGAAAAACTTGGAATTGAAGGAGCTATTCTCGTTGCCAACCCCATAC
CAGAACAATCCGAGATCCCAAGAGAAGAATTGGAAGAATACATAAAAAAAGCTGTCGCTCAAGCAAAGGAAAATGGTATT
TCAGGAAAATCCCTGACACCTTTCCTTCTAAGCAGATTGACAGAACTCTCAGGTGGCAAAACGCTCACCGCAAACATTGC
TTTACTCAAAAATAACGCCTCACTTGCCGGAGAGATAGCGGTGGCTCTTAGCAGGGGTGGTAGAGATTAA

Upstream 100 bases:

>100_bases
CACATTGGAGGCTTCCTTGTGGGTCTTTACATGGCCAAACGATTTGAGACAAAATATCCAGGATACTGGTATAGATAATT
TAAGCTGGAGGGATTGAAAA

Downstream 100 bases:

>100_bases
AAAAGTATGCTTCCGAAACGCAAAACTTTATGACCCAAAAATTGATTCATATATCAAAAGGGATTTACTCGTTGTAGACG
GAGTTATCGTCGAAGAAATC

Product: Indigoidine synthase A family protein

Products: NA

Alternate protein names: PsiMP glycosidase

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKKQPIVALESTVIAHGLPWPTNFETAVSMENKVKEKGAIPKTLGIINGEIKIGLTEKEIEHLAKAKNVMKIGTAEIAAA
IALRRNAATTVSATMRLAKNAGIDVFATGGIGGVHRNVPWDVSQDIMELSKTRMIVVCAGFKSILDVERTIEFLETFQVT
VVGYQTDYMPLFHTKESEYKVNIRADSPEEIVAIFNEKEKLGIEGAILVANPIPEQSEIPREELEEYIKKAVAQAKENGI
SGKSLTPFLLSRLTELSGGKTLTANIALLKNNASLAGEIAVALSRGGRD

Sequences:

>Translated_289_residues
MKKQPIVALESTVIAHGLPWPTNFETAVSMENKVKEKGAIPKTLGIINGEIKIGLTEKEIEHLAKAKNVMKIGTAEIAAA
IALRRNAATTVSATMRLAKNAGIDVFATGGIGGVHRNVPWDVSQDIMELSKTRMIVVCAGFKSILDVERTIEFLETFQVT
VVGYQTDYMPLFHTKESEYKVNIRADSPEEIVAIFNEKEKLGIEGAILVANPIPEQSEIPREELEEYIKKAVAQAKENGI
SGKSLTPFLLSRLTELSGGKTLTANIALLKNNASLAGEIAVALSRGGRD
>Mature_289_residues
MKKQPIVALESTVIAHGLPWPTNFETAVSMENKVKEKGAIPKTLGIINGEIKIGLTEKEIEHLAKAKNVMKIGTAEIAAA
IALRRNAATTVSATMRLAKNAGIDVFATGGIGGVHRNVPWDVSQDIMELSKTRMIVVCAGFKSILDVERTIEFLETFQVT
VVGYQTDYMPLFHTKESEYKVNIRADSPEEIVAIFNEKEKLGIEGAILVANPIPEQSEIPREELEEYIKKAVAQAKENGI
SGKSLTPFLLSRLTELSGGKTLTANIALLKNNASLAGEIAVALSRGGRD

Specific function: Catalyzes the hydrolysis of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil

COG id: COG2313

COG function: function code Q; Uncharacterized enzyme involved in pigment biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine-5'-phosphate glycosidase family

Homologues:

Organism=Escherichia coli, GI1788490, Length=286, Percent_Identity=48.2517482517483, Blast_Score=270, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI25147470, Length=282, Percent_Identity=43.9716312056738, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24580669, Length=288, Percent_Identity=44.0972222222222, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PSUG_KOSOT (C5CDZ1)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941097.1
- GeneID:   7968713
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1400
- ProtClustDB:   CLSK862888
- HAMAP:   MF_01876
- InterPro:   IPR022830
- InterPro:   IPR007342
- PANTHER:   PTHR10584:SF1

Pfam domain/function: PF04227 Indigoidine_A; SSF110581 Indigdn_synthA

EC number: NA

Molecular weight: Translated: 31323; Mature: 31323

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQPIVALESTVIAHGLPWPTNFETAVSMENKVKEKGAIPKTLGIINGEIKIGLTEKEI
CCCCCEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEECCEEEEECCHHHH
EHLAKAKNVMKIGTAEIAAAIALRRNAATTVSATMRLAKNAGIDVFATGGIGGVHRNVPW
HHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
DVSQDIMELSKTRMIVVCAGFKSILDVERTIEFLETFQVTVVGYQTDYMPLFHTKESEYK
CHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHEEEEEEEEECCCEEEEECCCCCEE
VNIRADSPEEIVAIFNEKEKLGIEGAILVANPIPEQSEIPREELEEYIKKAVAQAKENGI
EEEECCCHHHHHEEECCHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
SGKSLTPFLLSRLTELSGGKTLTANIALLKNNASLAGEIAVALSRGGRD
CCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHEEEEECCCCCC
>Mature Secondary Structure
MKKQPIVALESTVIAHGLPWPTNFETAVSMENKVKEKGAIPKTLGIINGEIKIGLTEKEI
CCCCCEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEECCEEEEECCHHHH
EHLAKAKNVMKIGTAEIAAAIALRRNAATTVSATMRLAKNAGIDVFATGGIGGVHRNVPW
HHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
DVSQDIMELSKTRMIVVCAGFKSILDVERTIEFLETFQVTVVGYQTDYMPLFHTKESEYK
CHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHEEEEEEEEECCCEEEEECCCCCEE
VNIRADSPEEIVAIFNEKEKLGIEGAILVANPIPEQSEIPREELEEYIKKAVAQAKENGI
EEEECCCHHHHHEEECCHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
SGKSLTPFLLSRLTELSGGKTLTANIALLKNNASLAGEIAVALSRGGRD
CCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA