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The map label for this gene is pycB [H]

Identifier: 239617770

GI number: 239617770

Start: 1485516

End: 1486913

Strand: Reverse

Name: pycB [H]

Synonym: Kole_1395

Alternate gene names: 239617770

Gene position: 1486913-1485516 (Counterclockwise)

Preceding gene: 239617772

Following gene: 239617769

Centisome position: 64.59

GC content: 43.63

Gene sequence:

>1398_bases
GTGGAAAAAGTTACTTTCACAGATACAACTTTTCGAGATGCCCATCAATCGTTGCTTGCTACAAGAGTTTCAACAAACGA
ACTAAAAGAAATTGCCCCCTTGGTTGATGAGGTAGGTTACCACGCTGTTGAAATCTGGGGCGGTGCCACCTATGACGCGT
GCGTTAGGTACCTTTTCGAAGATCCCTGGGAAAGACTTGATGAACTTCGTAAACGCTTCAAAAAAACGAAAATACAGATG
CTTCTTAGAGGTCAAAATCTTCTGGGATATAAACATTATGCAGATGATGTCGTTGAGTTGTTCGTAAAAAAGATGGCAGA
TCACGGCATGGACATAGTCAGAATCTTCGACGCATTAAACGACTTTAGAAATATCGAGAAGACCTGTAAAGAAGTAAAGA
AAAATGGAATGCACCTGCAGGTGGCGTTAGCTTACACCATCAGTCCCGTTCATAACGTGGAATATTTTGTTTCTTTAGCC
GAAAAAGCTGTTAATCTCAACGCAGATTCCCTTTGCATAAAAGATATGGCAGGCCTTTTAACACCCGGCAAAGCCTATGA
ACTTGTTAAAGCAATCAAAGAAAGATTCGACCTTCCACTGGAAATTCATGCACACTTCACCACGGGGGTTGCGGATATGT
CGTATATCAAAGCGGTCGAAGCCGGGGCGGATATCCTGGATACAGCCATAAGTCCCCTGGCTTACGGCACTTCACAGCCA
GCGGTTGAAAGCCTCTGGATTGCTCTGCACGACCTGGGAAATGCGTACGAACCTGATCTAAAGCTTCTTGAAAGAATAGC
AAACTATTTTGAAGAAGTGAGAAAACGGCATTCGGATACGGATGTGAAACTCACCACAACAAAACCAGCGATTCTCGTGA
ACCAGATCCCGGGTGGAATGTATTCCAACCTGATCAATCAGCTTAAAGGACAAAACGCGCTACATCTCTTAAACGACGTT
CTCGAAGAAGTTCCAAGAGTCAGAAAAGACCTTGGTTATCCACCGCTGGTGACACCCACATCCCAGATTGTTGGTGTTCA
GGCGGTGCTGAACGTCCTCTCAGGAGAGAGGTATAAGCTGATAACCAAAGAAGTGGAAAGGTATGTCAGAGGATATTATG
GAAAACCACCAGCAGAAATCTCACCCGAACTCAAAGAAAGAATTCTAAAGGATGAAAAAGCGATAACTTGCCGTCCTGCT
GATCTGCTGGAACCAGAGCTGGATAAAGCCCGGGAAAAAATAGGAATTCTCGCTAAAACTGATGAAGATCTGCTCACGTA
TATTCTCCTTGGAGAAGTAGGAAAGAAATACCTATTGAAAAAATATGAAAAAGAACTCGCAGTGGATTTCAGTACAGCCG
ATGAGTACTCAGATACCACTGCAGTTTACCCAATTTAA

Upstream 100 bases:

>100_bases
CACCAATAACCACTATATCAAAGTTGTAATCATTAATTGTTTTGGACATAACATCCCTCCTTTATTCTTATTAATTGTAC
AACAGAGGGGAGGAGTTAGC

Downstream 100 bases:

>100_bases
GTTATCTGAAGACGTTAGAGAGTCTTATAAATAGGAAATGACAAAAAACAACCGTTAATCAATTCTGAACGTCGTTAACG
CCAGATAATTGGAAATAGCT

Product: carboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MEKVTFTDTTFRDAHQSLLATRVSTNELKEIAPLVDEVGYHAVEIWGGATYDACVRYLFEDPWERLDELRKRFKKTKIQM
LLRGQNLLGYKHYADDVVELFVKKMADHGMDIVRIFDALNDFRNIEKTCKEVKKNGMHLQVALAYTISPVHNVEYFVSLA
EKAVNLNADSLCIKDMAGLLTPGKAYELVKAIKERFDLPLEIHAHFTTGVADMSYIKAVEAGADILDTAISPLAYGTSQP
AVESLWIALHDLGNAYEPDLKLLERIANYFEEVRKRHSDTDVKLTTTKPAILVNQIPGGMYSNLINQLKGQNALHLLNDV
LEEVPRVRKDLGYPPLVTPTSQIVGVQAVLNVLSGERYKLITKEVERYVRGYYGKPPAEISPELKERILKDEKAITCRPA
DLLEPELDKAREKIGILAKTDEDLLTYILLGEVGKKYLLKKYEKELAVDFSTADEYSDTTAVYPI

Sequences:

>Translated_465_residues
MEKVTFTDTTFRDAHQSLLATRVSTNELKEIAPLVDEVGYHAVEIWGGATYDACVRYLFEDPWERLDELRKRFKKTKIQM
LLRGQNLLGYKHYADDVVELFVKKMADHGMDIVRIFDALNDFRNIEKTCKEVKKNGMHLQVALAYTISPVHNVEYFVSLA
EKAVNLNADSLCIKDMAGLLTPGKAYELVKAIKERFDLPLEIHAHFTTGVADMSYIKAVEAGADILDTAISPLAYGTSQP
AVESLWIALHDLGNAYEPDLKLLERIANYFEEVRKRHSDTDVKLTTTKPAILVNQIPGGMYSNLINQLKGQNALHLLNDV
LEEVPRVRKDLGYPPLVTPTSQIVGVQAVLNVLSGERYKLITKEVERYVRGYYGKPPAEISPELKERILKDEKAITCRPA
DLLEPELDKAREKIGILAKTDEDLLTYILLGEVGKKYLLKKYEKELAVDFSTADEYSDTTAVYPI
>Mature_465_residues
MEKVTFTDTTFRDAHQSLLATRVSTNELKEIAPLVDEVGYHAVEIWGGATYDACVRYLFEDPWERLDELRKRFKKTKIQM
LLRGQNLLGYKHYADDVVELFVKKMADHGMDIVRIFDALNDFRNIEKTCKEVKKNGMHLQVALAYTISPVHNVEYFVSLA
EKAVNLNADSLCIKDMAGLLTPGKAYELVKAIKERFDLPLEIHAHFTTGVADMSYIKAVEAGADILDTAISPLAYGTSQP
AVESLWIALHDLGNAYEPDLKLLERIANYFEEVRKRHSDTDVKLTTTKPAILVNQIPGGMYSNLINQLKGQNALHLLNDV
LEEVPRVRKDLGYPPLVTPTSQIVGVQAVLNVLSGERYKLITKEVERYVRGYYGKPPAEISPELKERILKDEKAITCRPA
DLLEPELDKAREKIGILAKTDEDLLTYILLGEVGKKYLLKKYEKELAVDFSTADEYSDTTAVYPI

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=426, Percent_Identity=35.2112676056338, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI106049295, Length=426, Percent_Identity=35.2112676056338, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI106049292, Length=426, Percent_Identity=35.2112676056338, Blast_Score=239, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI17562816, Length=459, Percent_Identity=34.2047930283224, Blast_Score=239, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6319695, Length=431, Percent_Identity=35.4988399071926, Blast_Score=243, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6321376, Length=435, Percent_Identity=35.1724137931034, Blast_Score=240, Evalue=4e-64,
Organism=Drosophila melanogaster, GI281363050, Length=459, Percent_Identity=35.7298474945534, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24652224, Length=459, Percent_Identity=35.7298474945534, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24652222, Length=459, Percent_Identity=35.7298474945534, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24652220, Length=459, Percent_Identity=35.7298474945534, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24652218, Length=459, Percent_Identity=35.7298474945534, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24652212, Length=459, Percent_Identity=35.7298474945534, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24652210, Length=459, Percent_Identity=35.7298474945534, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24652214, Length=459, Percent_Identity=35.7298474945534, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI19921944, Length=459, Percent_Identity=35.7298474945534, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24652216, Length=459, Percent_Identity=35.7298474945534, Blast_Score=248, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 52553; Mature: 52553

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVTFTDTTFRDAHQSLLATRVSTNELKEIAPLVDEVGYHAVEIWGGATYDACVRYLFE
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHH
DPWERLDELRKRFKKTKIQMLLRGQNLLGYKHYADDVVELFVKKMADHGMDIVRIFDALN
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
DFRNIEKTCKEVKKNGMHLQVALAYTISPVHNVEYFVSLAEKAVNLNADSLCIKDMAGLL
HHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
TPGKAYELVKAIKERFDLPLEIHAHFTTGVADMSYIKAVEAGADILDTAISPLAYGTSQP
CCCHHHHHHHHHHHHCCCCEEEEEEHHHCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCH
AVESLWIALHDLGNAYEPDLKLLERIANYFEEVRKRHSDTDVKLTTTKPAILVNQIPGGM
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCHH
YSNLINQLKGQNALHLLNDVLEEVPRVRKDLGYPPLVTPTSQIVGVQAVLNVLSGERYKL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
ITKEVERYVRGYYGKPPAEISPELKERILKDEKAITCRPADLLEPELDKAREKIGILAKT
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEECCHHHCCCHHHHHHHHHCCCEEC
DEDLLTYILLGEVGKKYLLKKYEKELAVDFSTADEYSDTTAVYPI
CHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEECCC
>Mature Secondary Structure
MEKVTFTDTTFRDAHQSLLATRVSTNELKEIAPLVDEVGYHAVEIWGGATYDACVRYLFE
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHH
DPWERLDELRKRFKKTKIQMLLRGQNLLGYKHYADDVVELFVKKMADHGMDIVRIFDALN
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
DFRNIEKTCKEVKKNGMHLQVALAYTISPVHNVEYFVSLAEKAVNLNADSLCIKDMAGLL
HHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
TPGKAYELVKAIKERFDLPLEIHAHFTTGVADMSYIKAVEAGADILDTAISPLAYGTSQP
CCCHHHHHHHHHHHHCCCCEEEEEEHHHCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCH
AVESLWIALHDLGNAYEPDLKLLERIANYFEEVRKRHSDTDVKLTTTKPAILVNQIPGGM
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCHH
YSNLINQLKGQNALHLLNDVLEEVPRVRKDLGYPPLVTPTSQIVGVQAVLNVLSGERYKL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
ITKEVERYVRGYYGKPPAEISPELKERILKDEKAITCRPADLLEPELDKAREKIGILAKT
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEECCHHHCCCHHHHHHHHHCCCEEC
DEDLLTYILLGEVGKKYLLKKYEKELAVDFSTADEYSDTTAVYPI
CHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]