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The map label for this gene is grpE [H]

Identifier: 239617760

GI number: 239617760

Start: 1473007

End: 1473627

Strand: Direct

Name: grpE [H]

Synonym: Kole_1385

Alternate gene names: 239617760

Gene position: 1473007-1473627 (Clockwise)

Preceding gene: 239617759

Following gene: 239617761

Centisome position: 63.98

GC content: 39.29

Gene sequence:

>621_bases
ATGTCAGAAGAAGCCAGAAAGAATTTGAAAAAGAGTGTTGAGAATAAAGGAGAGTTTGAAAAAAAGATAAAGAAGGAAGC
CTCGGAAAAGGAAGTTGAAAGGCTTAGGAAAACGCTTGAGGAAAAAGAGAAAGAGATAAAAACATTGAAAGATGATATAT
CCCGTTTGCGTTCAGAGTTTCACAATTTTAAACTCGCTCTGGAGAGGGAAACCAATCGACTCGTTTTGAAGCAAAAGGAA
AATATGATTTTCAAGTTGTTGGATCTGAAGGAAGATTTTGAGAGGGCTATGAATCATTTTAGTGAAGAAGCCAGCCCATT
GATTAAGGGTGTTAGGATGATTTACAAGAAGTTGGAAAGTATCCTCAAAGAGGAGGGAGTAGAAGAAATCGTTCCAACGG
TTGGCGAGCTTTTCGATCCGTTCAGGGATGAGGTTGCCGAAACCGTTGAATCTGATGTTGTTGAAGATATGTCTATTCTG
GAAGTAGCTGAGAAAGGCTATAAATTTTCCGGTAAGATTATAAAACCCCCGCGTGTTATTGTTGCTATGAAGCCCAAAAA
AGCTGATAGTAAAAAGGAAAACCCTGAAGACGAGGGAGGAAAAGAGTCGAAGAAACGGTAG

Upstream 100 bases:

>100_bases
AAAATAAACCTTTATGAAACAACGTACGCGTATTTGCGATTCGCTTCGAATAGGCTTTCGGAAGCGTTTAGCAAGAGGTA
GTGAGTCAGGAGGTGATAAT

Downstream 100 bases:

>100_bases
GTGATGTAAATGGCTGCTGCGAAAAAGGATTATTATGAGGTTCTGGGTGTGTCCCGTGGGGCATCCCAGGAAGAAATAAA
AAAGGCATACAGAAAATTGG

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSEEARKNLKKSVENKGEFEKKIKKEASEKEVERLRKTLEEKEKEIKTLKDDISRLRSEFHNFKLALERETNRLVLKQKE
NMIFKLLDLKEDFERAMNHFSEEASPLIKGVRMIYKKLESILKEEGVEEIVPTVGELFDPFRDEVAETVESDVVEDMSIL
EVAEKGYKFSGKIIKPPRVIVAMKPKKADSKKENPEDEGGKESKKR

Sequences:

>Translated_206_residues
MSEEARKNLKKSVENKGEFEKKIKKEASEKEVERLRKTLEEKEKEIKTLKDDISRLRSEFHNFKLALERETNRLVLKQKE
NMIFKLLDLKEDFERAMNHFSEEASPLIKGVRMIYKKLESILKEEGVEEIVPTVGELFDPFRDEVAETVESDVVEDMSIL
EVAEKGYKFSGKIIKPPRVIVAMKPKKADSKKENPEDEGGKESKKR
>Mature_205_residues
SEEARKNLKKSVENKGEFEKKIKKEASEKEVERLRKTLEEKEKEIKTLKDDISRLRSEFHNFKLALERETNRLVLKQKEN
MIFKLLDLKEDFERAMNHFSEEASPLIKGVRMIYKKLESILKEEGVEEIVPTVGELFDPFRDEVAETVESDVVEDMSILE
VAEKGYKFSGKIIKPPRVIVAMKPKKADSKKENPEDEGGKESKKR

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324806, Length=164, Percent_Identity=29.2682926829268, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 24029; Mature: 23898

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEARKNLKKSVENKGEFEKKIKKEASEKEVERLRKTLEEKEKEIKTLKDDISRLRSEF
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HNFKLALERETNRLVLKQKENMIFKLLDLKEDFERAMNHFSEEASPLIKGVRMIYKKLES
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKEEGVEEIVPTVGELFDPFRDEVAETVESDVVEDMSILEVAEKGYKFSGKIIKPPRVI
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEE
VAMKPKKADSKKENPEDEGGKESKKR
EEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEEARKNLKKSVENKGEFEKKIKKEASEKEVERLRKTLEEKEKEIKTLKDDISRLRSEF
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HNFKLALERETNRLVLKQKENMIFKLLDLKEDFERAMNHFSEEASPLIKGVRMIYKKLES
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKEEGVEEIVPTVGELFDPFRDEVAETVESDVVEDMSILEVAEKGYKFSGKIIKPPRVI
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEE
VAMKPKKADSKKENPEDEGGKESKKR
EEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]