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The map label for this gene is wcaJ [H]

Identifier: 239617666

GI number: 239617666

Start: 1375168

End: 1376454

Strand: Reverse

Name: wcaJ [H]

Synonym: Kole_1290

Alternate gene names: 239617666

Gene position: 1376454-1375168 (Counterclockwise)

Preceding gene: 239617672

Following gene: 239617665

Centisome position: 59.79

GC content: 37.14

Gene sequence:

>1287_bases
TTGAAAAGATTACGCGAAGTGAAATATTCGTTAGCATTGTTCTATTTTATAATTTTATACATCATTCAATCCCTGTTACT
CGAGTGGGATATTTCTCTTTTTATCCAGGCGACAATCTTAAACATTTCACTTCTCTTAGGCATCTACGCATTCCGGGGCT
TTGATAATTTCAACATTCAATCATTAAATTCTAGTCTTGTCTCTTATTCTATAGGCTCTCTTATAGGTGCTCTCTTCTCT
TTGATGATCGTAGCTTTTTTCAAAGCAAAACTTCCAAAAGAGGTGTTCATAACAGCTCTTGCACTTTCAGGTGTGATATT
TCCAATAATCTCTACATTCACAATAAGAAAAGTGCTTTCCATGATCCCTCCAAAACGCTATCTCGTTATTGGGAATGAGT
CAGAGATAGGAGAAATCATCCGAGAAATCGAAAAAGCTTCTATGGAAAAAGTAAAAATCTATGGATATATGAATCCTTCT
TCTGTTGCTCTTGAACAAGCTCTTTCACTAAGTCTCTTTGATAGCATCCTCGTAGCCGACCAAAAGCTCTCCAGATCTGT
AGAATCCATTCTCGAAAAAGCAGATTCACAGGGTAAATCAGTGGAATTCCTTCCCTTAATAGTCGAACAAACCCTGAAAA
GAATCCCCATTGAACTGATAGATAAGTTCAAGGAGTATTATGAAATTCAATTCAGTTCAGTAAAAGAAACCCCTTCAAAA
AGAGTTTTAGACCTATTCATTTCAGTAATAGCTATGATTATTCTTTCCCCTGTATATTTGGTAATATATCTGGCAATACT
TCTGGACGATGGACTACCAATCATTTTCAAACAAATGCGCGTAGGCTTGAATGGCAGTAATTTTATCATGCATAAATTCA
GGTCAATGAAAAAAGATGCGGAATCAACCGGTGCTAAATATGCCACCCAACAGGAAAACAACGTCACAAGAATCGGAAAA
TTAATGAGACCAGTCAGATTGGATGAAATTCCCCAGTTTTATGATATATTGATAGGAAAGATGTCCTTCGTGGGTCCAAG
GCCAGAACAACCTGAATTCGCCAAAGAATTGGCAGAAGCTTTACCCTACTATAACTACCGCAATAAATTAAAACCCGGAC
TTACCGGTTGGGCTCAGACGAATTACAAATACGCTGCAACGATAGAAGAACAGGCAAAGAAACTAAGCTATGACCTTTAC
TACATAAAAAACAGATCGTTACTGCTCGATCTTCTGATAATTCTTAGAACGTTTGAAACTGTGGTATTCAGGAAGGGAGC
TAAGTAA

Upstream 100 bases:

>100_bases
ACAAGTATTAACTGAGGGTAAAGACGTCACTGGTAACGGTAAACCAGAGCAAATATTTAAATAGCAAGCTTTTTATTAAT
TTATTATCGGAGGGGGCTAT

Downstream 100 bases:

>100_bases
ACATAATATGATGAAAACAAAGGAGCTCTCATAATTCCAGTGGTAATGCTATCAAGATAATCTCAAGTAAAGATAGTAGA
CCCGTAAAAAAGGCACCTAA

Product: exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKRLREVKYSLALFYFIILYIIQSLLLEWDISLFIQATILNISLLLGIYAFRGFDNFNIQSLNSSLVSYSIGSLIGALFS
LMIVAFFKAKLPKEVFITALALSGVIFPIISTFTIRKVLSMIPPKRYLVIGNESEIGEIIREIEKASMEKVKIYGYMNPS
SVALEQALSLSLFDSILVADQKLSRSVESILEKADSQGKSVEFLPLIVEQTLKRIPIELIDKFKEYYEIQFSSVKETPSK
RVLDLFISVIAMIILSPVYLVIYLAILLDDGLPIIFKQMRVGLNGSNFIMHKFRSMKKDAESTGAKYATQQENNVTRIGK
LMRPVRLDEIPQFYDILIGKMSFVGPRPEQPEFAKELAEALPYYNYRNKLKPGLTGWAQTNYKYAATIEEQAKKLSYDLY
YIKNRSLLLDLLIILRTFETVVFRKGAK

Sequences:

>Translated_428_residues
MKRLREVKYSLALFYFIILYIIQSLLLEWDISLFIQATILNISLLLGIYAFRGFDNFNIQSLNSSLVSYSIGSLIGALFS
LMIVAFFKAKLPKEVFITALALSGVIFPIISTFTIRKVLSMIPPKRYLVIGNESEIGEIIREIEKASMEKVKIYGYMNPS
SVALEQALSLSLFDSILVADQKLSRSVESILEKADSQGKSVEFLPLIVEQTLKRIPIELIDKFKEYYEIQFSSVKETPSK
RVLDLFISVIAMIILSPVYLVIYLAILLDDGLPIIFKQMRVGLNGSNFIMHKFRSMKKDAESTGAKYATQQENNVTRIGK
LMRPVRLDEIPQFYDILIGKMSFVGPRPEQPEFAKELAEALPYYNYRNKLKPGLTGWAQTNYKYAATIEEQAKKLSYDLY
YIKNRSLLLDLLIILRTFETVVFRKGAK
>Mature_428_residues
MKRLREVKYSLALFYFIILYIIQSLLLEWDISLFIQATILNISLLLGIYAFRGFDNFNIQSLNSSLVSYSIGSLIGALFS
LMIVAFFKAKLPKEVFITALALSGVIFPIISTFTIRKVLSMIPPKRYLVIGNESEIGEIIREIEKASMEKVKIYGYMNPS
SVALEQALSLSLFDSILVADQKLSRSVESILEKADSQGKSVEFLPLIVEQTLKRIPIELIDKFKEYYEIQFSSVKETPSK
RVLDLFISVIAMIILSPVYLVIYLAILLDDGLPIIFKQMRVGLNGSNFIMHKFRSMKKDAESTGAKYATQQENNVTRIGK
LMRPVRLDEIPQFYDILIGKMSFVGPRPEQPEFAKELAEALPYYNYRNKLKPGLTGWAQTNYKYAATIEEQAKKLSYDLY
YIKNRSLLLDLLIILRTFETVVFRKGAK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=180, Percent_Identity=37.7777777777778, Blast_Score=135, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 48907; Mature: 48907

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLREVKYSLALFYFIILYIIQSLLLEWDISLFIQATILNISLLLGIYAFRGFDNFNIQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
SLNSSLVSYSIGSLIGALFSLMIVAFFKAKLPKEVFITALALSGVIFPIISTFTIRKVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIPPKRYLVIGNESEIGEIIREIEKASMEKVKIYGYMNPSSVALEQALSLSLFDSILVAD
HCCCCEEEEECCCHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
QKLSRSVESILEKADSQGKSVEFLPLIVEQTLKRIPIELIDKFKEYYEIQFSSVKETPSK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHH
RVLDLFISVIAMIILSPVYLVIYLAILLDDGLPIIFKQMRVGLNGSNFIMHKFRSMKKDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ESTGAKYATQQENNVTRIGKLMRPVRLDEIPQFYDILIGKMSFVGPRPEQPEFAKELAEA
HHCCCCCCCCCCCCHHHHHHHHCCCCHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LPYYNYRNKLKPGLTGWAQTNYKYAATIEEQAKKLSYDLYYIKNRSLLLDLLIILRTFET
CCCCCCHHCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHH
VVFRKGAK
HHHHCCCC
>Mature Secondary Structure
MKRLREVKYSLALFYFIILYIIQSLLLEWDISLFIQATILNISLLLGIYAFRGFDNFNIQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
SLNSSLVSYSIGSLIGALFSLMIVAFFKAKLPKEVFITALALSGVIFPIISTFTIRKVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIPPKRYLVIGNESEIGEIIREIEKASMEKVKIYGYMNPSSVALEQALSLSLFDSILVAD
HCCCCEEEEECCCHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
QKLSRSVESILEKADSQGKSVEFLPLIVEQTLKRIPIELIDKFKEYYEIQFSSVKETPSK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHH
RVLDLFISVIAMIILSPVYLVIYLAILLDDGLPIIFKQMRVGLNGSNFIMHKFRSMKKDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ESTGAKYATQQENNVTRIGKLMRPVRLDEIPQFYDILIGKMSFVGPRPEQPEFAKELAEA
HHCCCCCCCCCCCCHHHHHHHHCCCCHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LPYYNYRNKLKPGLTGWAQTNYKYAATIEEQAKKLSYDLYYIKNRSLLLDLLIILRTFET
CCCCCCHHCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHH
VVFRKGAK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]