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The map label for this gene is braC [H]

Identifier: 239617486

GI number: 239617486

Start: 1170511

End: 1171614

Strand: Direct

Name: braC [H]

Synonym: Kole_1102

Alternate gene names: 239617486

Gene position: 1170511-1171614 (Clockwise)

Preceding gene: 239617484

Following gene: 239617487

Centisome position: 50.84

GC content: 46.01

Gene sequence:

>1104_bases
GTGAGAAGACTAGTTGTAGTATTGTTGATTTTATCCATGGGGATTATATCGCTTGGGGCGATCAAGATAGGGGTTGTCTT
GCCGATGACAGGCGGGATTGCCGCTTTTGGAAGAATGGTCTGGGAAGGAGTAGAGATTGCACGTGAATTGTTTCCTACCG
CTCTTGGACAGGAAATAGAGCTTGTGTTGCTTGACAATCGTAGTGACAAGGTTGAAGCTGCCAACGCCGTAAGGCGTGCA
ATTGAACACGAAAAAGTGCTGGCAATTCTTGGGGAAATTGCGAGTTCTCACACTCTGGCTGGAGGGGCTGTTGCTGAAGA
AAAACGTACTCCGATGGTTTCACCTTCTTCGACCAATCCTCTTGTGACCTCAGGGAAAAAGTATGTTTCTCGTGTGTGTT
TTATCGATCCATTCCAGGGTTGGGCTGCAGCCAAACTCGCTTTTGAAAATCTTGATGCGAGAAACGTTGCGATCTTCATG
GATGTTGAACAGGACTATGCTGTTGGTCTGGCAAACTTCTTCAAAAAGACCTTCCTTGAACTTGGAGGCACTGTTTTCTT
CGAATACTACAAAACTGGAGACCAGGATTTCTCTGCACAGGTGTATGACGCTCTTATGAGAGGTGCCGATGCTTTCTTCA
TTCCCGGGTACTACCAGGAGATTGCACTTATAGCTATTCAGGCAAGGCAACTGGGTTTCTACGGTCCGCTCGTAACCGGT
GACGGTGCGGATGCTCCCGAAACTATCTCCATCGGTGGAGATGCTGTCAATGGTCTTTACTTCACAACCCATTACCATCC
AGATAGTCCAGCTGTTACAGAAAATGCCAAGCTCTTCTTAGAAAAGTTCAGAGAAAAGTATGGAAAAGAACCGGCAGCTC
TCAGTGCTCTTGGTTTTGATGCGTACATGGTTATAAGGGATGCCATAATCAGGGCTGGATCTCTCGATAAAGATGCTATC
GCGGAAGAAATAAGAAAAACGAAGAACTTCCCGGGAGCTACCGGAATCATCAACATCGATTCCAACGGGGATGCGGTAAA
ATCTGTGGCAATAGTAAAAATAGAAGACGGCAAATTCAAGTACGAGACCACTATAAATCCATAA

Upstream 100 bases:

>100_bases
TTTGAAATGCTCTGATTATTTTAATTTAAAGCCTCCCTATGGTATACTTTCAGCTAAAATACGGCAAAGATTAGCCACTA
TCTATTGGGGAGGTGTTGTT

Downstream 100 bases:

>100_bases
CATGATTTAAGAGAGGGAATGCACATTCTTCCCTCTCTTTTCCCGTTTTTCTTTTCTTTTTTTTCACTTCATCTTTCTTC
TGTGATGTTCGGAGGTGGAC

Product: Extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MRRLVVVLLILSMGIISLGAIKIGVVLPMTGGIAAFGRMVWEGVEIARELFPTALGQEIELVLLDNRSDKVEAANAVRRA
IEHEKVLAILGEIASSHTLAGGAVAEEKRTPMVSPSSTNPLVTSGKKYVSRVCFIDPFQGWAAAKLAFENLDARNVAIFM
DVEQDYAVGLANFFKKTFLELGGTVFFEYYKTGDQDFSAQVYDALMRGADAFFIPGYYQEIALIAIQARQLGFYGPLVTG
DGADAPETISIGGDAVNGLYFTTHYHPDSPAVTENAKLFLEKFREKYGKEPAALSALGFDAYMVIRDAIIRAGSLDKDAI
AEEIRKTKNFPGATGIINIDSNGDAVKSVAIVKIEDGKFKYETTINP

Sequences:

>Translated_367_residues
MRRLVVVLLILSMGIISLGAIKIGVVLPMTGGIAAFGRMVWEGVEIARELFPTALGQEIELVLLDNRSDKVEAANAVRRA
IEHEKVLAILGEIASSHTLAGGAVAEEKRTPMVSPSSTNPLVTSGKKYVSRVCFIDPFQGWAAAKLAFENLDARNVAIFM
DVEQDYAVGLANFFKKTFLELGGTVFFEYYKTGDQDFSAQVYDALMRGADAFFIPGYYQEIALIAIQARQLGFYGPLVTG
DGADAPETISIGGDAVNGLYFTTHYHPDSPAVTENAKLFLEKFREKYGKEPAALSALGFDAYMVIRDAIIRAGSLDKDAI
AEEIRKTKNFPGATGIINIDSNGDAVKSVAIVKIEDGKFKYETTINP
>Mature_367_residues
MRRLVVVLLILSMGIISLGAIKIGVVLPMTGGIAAFGRMVWEGVEIARELFPTALGQEIELVLLDNRSDKVEAANAVRRA
IEHEKVLAILGEIASSHTLAGGAVAEEKRTPMVSPSSTNPLVTSGKKYVSRVCFIDPFQGWAAAKLAFENLDARNVAIFM
DVEQDYAVGLANFFKKTFLELGGTVFFEYYKTGDQDFSAQVYDALMRGADAFFIPGYYQEIALIAIQARQLGFYGPLVTG
DGADAPETISIGGDAVNGLYFTTHYHPDSPAVTENAKLFLEKFREKYGKEPAALSALGFDAYMVIRDAIIRAGSLDKDAI
AEEIRKTKNFPGATGIINIDSNGDAVKSVAIVKIEDGKFKYETTINP

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=330, Percent_Identity=26.969696969697, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI48994941, Length=247, Percent_Identity=27.5303643724696, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39840; Mature: 39840

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLVVVLLILSMGIISLGAIKIGVVLPMTGGIAAFGRMVWEGVEIARELFPTALGQEIE
CHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LVLLDNRSDKVEAANAVRRAIEHEKVLAILGEIASSHTLAGGAVAEEKRTPMVSPSSTNP
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCC
LVTSGKKYVSRVCFIDPFQGWAAAKLAFENLDARNVAIFMDVEQDYAVGLANFFKKTFLE
CEECCHHHHHHHHEECCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
LGGTVFFEYYKTGDQDFSAQVYDALMRGADAFFIPGYYQEIALIAIQARQLGFYGPLVTG
CCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHEEEHHHCCCCCCEEEC
DGADAPETISIGGDAVNGLYFTTHYHPDSPAVTENAKLFLEKFREKYGKEPAALSALGFD
CCCCCCCEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCH
AYMVIRDAIIRAGSLDKDAIAEEIRKTKNFPGATGIINIDSNGDAVKSVAIVKIEDGKFK
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEEEECCCEE
YETTINP
EEECCCC
>Mature Secondary Structure
MRRLVVVLLILSMGIISLGAIKIGVVLPMTGGIAAFGRMVWEGVEIARELFPTALGQEIE
CHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LVLLDNRSDKVEAANAVRRAIEHEKVLAILGEIASSHTLAGGAVAEEKRTPMVSPSSTNP
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCC
LVTSGKKYVSRVCFIDPFQGWAAAKLAFENLDARNVAIFMDVEQDYAVGLANFFKKTFLE
CEECCHHHHHHHHEECCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
LGGTVFFEYYKTGDQDFSAQVYDALMRGADAFFIPGYYQEIALIAIQARQLGFYGPLVTG
CCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHEEEHHHCCCCCCEEEC
DGADAPETISIGGDAVNGLYFTTHYHPDSPAVTENAKLFLEKFREKYGKEPAALSALGFD
CCCCCCCEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCH
AYMVIRDAIIRAGSLDKDAIAEEIRKTKNFPGATGIINIDSNGDAVKSVAIVKIEDGKFK
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEEEECCCEE
YETTINP
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]