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The map label for this gene is rpfG [H]

Identifier: 239617404

GI number: 239617404

Start: 1075393

End: 1076490

Strand: Reverse

Name: rpfG [H]

Synonym: Kole_1015

Alternate gene names: 239617404

Gene position: 1076490-1075393 (Counterclockwise)

Preceding gene: 239617405

Following gene: 239617402

Centisome position: 46.76

GC content: 39.16

Gene sequence:

>1098_bases
ATGAAAATTCTGGCTGTTGATGATAACAAAGAAAATATCTATATGTTAGAGACTTTGTTCAAGAGCTATGGTTATGAAGT
TGAATCTTCCCAGAATGGTGTTGAAGCTCTGGAGAAATTGGCCGAACAGGATTTTGACGTGGTAATTTCCGATATTTTGA
TGCCCGAGATGGATGGTTTTCAATTATGTCGCAGGGTGAAAATGGATGATAGATTAAAAGAAATCCCTTTTATACTTTAT
ACCGCTGCCTACACCGATCCAGAAGATAGAAAATTCGCCCTCAGCCTTGGAGTCGATAGGTTTGTTGTCAAGCCATTAGA
ACCAGCTACTTTTGTTGAGATAATAAAAGAAGTTATTCAGAACCATAAAGTAAAACCTTCAAACAACAAGGTAGTAAATA
AGGAAGAGATTGTATATCTTAAGAAATACAACGAGAGATTGATCAAGAAATTGGAAGATGGAATGCTTCAGTTAGAAGAA
AGAAACAAAGAACTCGAAAAAAGCTATAAAAAGGTACAGAGAACCCTGGATTCCGTTGTTGAAACAATAGGATTGGTAAT
TGAAATGAGAGATGCCTACACTTCTGGTCATCAACGACGCGTAAGTCAGCTTGCCTGCGCTATTGCCAGAGAAATGAACC
TGTCAGAAGAACAAATTGAGGCTGTTCGTATAGCTGGTCTTTTACATGATATTGGCAAGATATCCATACCGACAGATATC
TTGAACAAGCCTGACAAACTGAATAAATTTGAACTCGGAATTGTAAGAAACCATCCACAAACTGCATTTGAAATATTAAA
AGACGTGGAATTTTCCCAGCCCATAGCTGAGATTGTGCTCCAGCATCATGAACGAATAGATGGATCCGGTTATCCTAATG
GCTTGAAGGGTAACCAAATTCTCACAGAAGCGAAGATACTAGCAGTGTCAGATGTTGTTGAAGCCATGGCGTTCCACAGA
CCTTATCGTCCGGCACTTGGTATAGCTAAAGCTTTAGAGGAGATCACCACAAATTCAGGTAACCTCTACGACCCTGAGGC
TGTAAAAGCTTGCATAGCAGTGTTCTCACGAGGATTTGATTTTGAAAAAGAGAGGTAA

Upstream 100 bases:

>100_bases
GCCATCGGCTGTGTTGGATACATCAAGAAACCATTTTTTCCTGAGAATTTCATACCAGAAATAGAGAAATATCTTTTCGA
AAATAAGGAAAGTGATAAAC

Downstream 100 bases:

>100_bases
AAAGCTTACATTATGAAAACGGGAGTCTTTTGACTCCCGTTTTTTATTTTCTTTTTACTGGAACTTTTTTCAGATTTGCA
TATACCGGTATCCCTCGAAG

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKILAVDDNKENIYMLETLFKSYGYEVESSQNGVEALEKLAEQDFDVVISDILMPEMDGFQLCRRVKMDDRLKEIPFILY
TAAYTDPEDRKFALSLGVDRFVVKPLEPATFVEIIKEVIQNHKVKPSNNKVVNKEEIVYLKKYNERLIKKLEDGMLQLEE
RNKELEKSYKKVQRTLDSVVETIGLVIEMRDAYTSGHQRRVSQLACAIAREMNLSEEQIEAVRIAGLLHDIGKISIPTDI
LNKPDKLNKFELGIVRNHPQTAFEILKDVEFSQPIAEIVLQHHERIDGSGYPNGLKGNQILTEAKILAVSDVVEAMAFHR
PYRPALGIAKALEEITTNSGNLYDPEAVKACIAVFSRGFDFEKER

Sequences:

>Translated_365_residues
MKILAVDDNKENIYMLETLFKSYGYEVESSQNGVEALEKLAEQDFDVVISDILMPEMDGFQLCRRVKMDDRLKEIPFILY
TAAYTDPEDRKFALSLGVDRFVVKPLEPATFVEIIKEVIQNHKVKPSNNKVVNKEEIVYLKKYNERLIKKLEDGMLQLEE
RNKELEKSYKKVQRTLDSVVETIGLVIEMRDAYTSGHQRRVSQLACAIAREMNLSEEQIEAVRIAGLLHDIGKISIPTDI
LNKPDKLNKFELGIVRNHPQTAFEILKDVEFSQPIAEIVLQHHERIDGSGYPNGLKGNQILTEAKILAVSDVVEAMAFHR
PYRPALGIAKALEEITTNSGNLYDPEAVKACIAVFSRGFDFEKER
>Mature_365_residues
MKILAVDDNKENIYMLETLFKSYGYEVESSQNGVEALEKLAEQDFDVVISDILMPEMDGFQLCRRVKMDDRLKEIPFILY
TAAYTDPEDRKFALSLGVDRFVVKPLEPATFVEIIKEVIQNHKVKPSNNKVVNKEEIVYLKKYNERLIKKLEDGMLQLEE
RNKELEKSYKKVQRTLDSVVETIGLVIEMRDAYTSGHQRRVSQLACAIAREMNLSEEQIEAVRIAGLLHDIGKISIPTDI
LNKPDKLNKFELGIVRNHPQTAFEILKDVEFSQPIAEIVLQHHERIDGSGYPNGLKGNQILTEAKILAVSDVVEAMAFHR
PYRPALGIAKALEEITTNSGNLYDPEAVKACIAVFSRGFDFEKER

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=107, Percent_Identity=35.5140186915888, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=33.0578512396694, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788713, Length=106, Percent_Identity=33.9622641509434, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693157, Length=119, Percent_Identity=32.7731092436975, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789149, Length=107, Percent_Identity=30.8411214953271, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788550, Length=124, Percent_Identity=34.6774193548387, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=28.9256198347107, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 41631; Mature: 41631

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILAVDDNKENIYMLETLFKSYGYEVESSQNGVEALEKLAEQDFDVVISDILMPEMDGF
CEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
QLCRRVKMDDRLKEIPFILYTAAYTDPEDRKFALSLGVDRFVVKPLEPATFVEIIKEVIQ
HHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHH
NHKVKPSNNKVVNKEEIVYLKKYNERLIKKLEDGMLQLEERNKELEKSYKKVQRTLDSVV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETIGLVIEMRDAYTSGHQRRVSQLACAIAREMNLSEEQIEAVRIAGLLHDIGKISIPTDI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
LNKPDKLNKFELGIVRNHPQTAFEILKDVEFSQPIAEIVLQHHERIDGSGYPNGLKGNQI
CCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHH
LTEAKILAVSDVVEAMAFHRPYRPALGIAKALEEITTNSGNLYDPEAVKACIAVFSRGFD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
FEKER
CCCCC
>Mature Secondary Structure
MKILAVDDNKENIYMLETLFKSYGYEVESSQNGVEALEKLAEQDFDVVISDILMPEMDGF
CEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
QLCRRVKMDDRLKEIPFILYTAAYTDPEDRKFALSLGVDRFVVKPLEPATFVEIIKEVIQ
HHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHH
NHKVKPSNNKVVNKEEIVYLKKYNERLIKKLEDGMLQLEERNKELEKSYKKVQRTLDSVV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETIGLVIEMRDAYTSGHQRRVSQLACAIAREMNLSEEQIEAVRIAGLLHDIGKISIPTDI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
LNKPDKLNKFELGIVRNHPQTAFEILKDVEFSQPIAEIVLQHHERIDGSGYPNGLKGNQI
CCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHH
LTEAKILAVSDVVEAMAFHRPYRPALGIAKALEEITTNSGNLYDPEAVKACIAVFSRGFD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
FEKER
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]