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The map label for this gene is thiF [H]

Identifier: 239617388

GI number: 239617388

Start: 1055252

End: 1056352

Strand: Direct

Name: thiF [H]

Synonym: Kole_0998

Alternate gene names: 239617388

Gene position: 1055252-1056352 (Clockwise)

Preceding gene: 239617365

Following gene: 239617389

Centisome position: 45.84

GC content: 40.87

Gene sequence:

>1101_bases
TTGGATGATACGGTTATCCTCACCTTATATGAAAGACAGATTCTGGTAGAAGGTATTGATATTGAATTTCAACAAAGACT
CACAGAGCAGGTTTTGGGAGTTCCGAGAATACCTGCCGAAAGGTTGATAGTAAGGTTATTGTTCAGGTCAGGGTTGCTTG
GGGTTTCCCAAGACTATTCCTGGGATGAAATGAAAGTTCTTTCGGGGATTAATTCTTATATTTTTCCTTCTTCAGAAACT
ACGGTTATTTTCGAAAATTCAAGTGAGATGTCAGTTGAGTATGACAACACCAATGATCGGGTTACAGTAAAATCCCCGGC
AAATATTTCGGATATAGCCGCGGTAATACTGGCAAGCAAGGTCTTCATGGCTTTTTTGAAGAAGGAAGGCACAAAGCTTT
CCCCGGATATAATACCGGATTTTGGCAGATTTTCAATCCCGGAAAACAAAAAGGTAATGGTGGTCGGAGCTGGAGGGCTT
GGATCACCGGTAATTGAATATTTGCTCCGTTGGGGATTGAAAAAGCTGGTGGTTGTCGACAATGATTGGGTGAATGTATC
CAACCTTCACAGACAGACGTTGTATTTCTACGAAGATATTGGGAAGCCAAAGGTCAAGGTTTTAGAGAAAAGGATTTCCC
GTTTCATGCAGGACGTTGAAATCATCGGTTATGAGACAACATTTTGTGGAGAAATGTTGTCAAAAGAGAAACCAGATGTT
GTTGTTTCAGCGGTGGATAATTTCAGGACTCGTTATGAAATCAACTATGAGTGTTATCGCAGGTACGTACCTTTTATCGA
CGCTGGTGTGTCTGGATACGAGGGTTATGTGATGAGACATACACCTGAAGAATCTTGTTACAGATGCTTTGTTGGTGATG
ATAAAAAGGATAAAGAAGGTAAAAAGCCAATTCTACCATTTACCAGCTATTTGGGTGGACTGCTGCAGGCTTACTGGGTA
TCTGCATTGTTAAATAATAAGAAATTGCCTCAAGCGTATTGGTTTGATCTGAAGAACATGATTTTCAGTTCTTTTGAGGT
TCCAAAAAAGGAAACATGTCCTGTTTGTGGGAGGAATAGCCGTGGACGTCGTTTATGGTGA

Upstream 100 bases:

>100_bases
TATTGTCTATGGCACTTTTTTGTTGTACATCCGGGAGGGTATATGGTTTCTTTATTCATGTTAAAATTCAATTGAGAAGA
GAGGGAAGGAGGGGGGTAAT

Downstream 100 bases:

>100_bases
TAAGAAGTGGAAATTCAACGAATCCATGACAGTTAAAGAACTTCTGGAAAAGACCGGTTTGAGAAGAGATCCGGTACTTG
TTATGGCGGATGGACGTTTG

Product: UBA/THIF-type NAD/FAD binding protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MDDTVILTLYERQILVEGIDIEFQQRLTEQVLGVPRIPAERLIVRLLFRSGLLGVSQDYSWDEMKVLSGINSYIFPSSET
TVIFENSSEMSVEYDNTNDRVTVKSPANISDIAAVILASKVFMAFLKKEGTKLSPDIIPDFGRFSIPENKKVMVVGAGGL
GSPVIEYLLRWGLKKLVVVDNDWVNVSNLHRQTLYFYEDIGKPKVKVLEKRISRFMQDVEIIGYETTFCGEMLSKEKPDV
VVSAVDNFRTRYEINYECYRRYVPFIDAGVSGYEGYVMRHTPEESCYRCFVGDDKKDKEGKKPILPFTSYLGGLLQAYWV
SALLNNKKLPQAYWFDLKNMIFSSFEVPKKETCPVCGRNSRGRRLW

Sequences:

>Translated_366_residues
MDDTVILTLYERQILVEGIDIEFQQRLTEQVLGVPRIPAERLIVRLLFRSGLLGVSQDYSWDEMKVLSGINSYIFPSSET
TVIFENSSEMSVEYDNTNDRVTVKSPANISDIAAVILASKVFMAFLKKEGTKLSPDIIPDFGRFSIPENKKVMVVGAGGL
GSPVIEYLLRWGLKKLVVVDNDWVNVSNLHRQTLYFYEDIGKPKVKVLEKRISRFMQDVEIIGYETTFCGEMLSKEKPDV
VVSAVDNFRTRYEINYECYRRYVPFIDAGVSGYEGYVMRHTPEESCYRCFVGDDKKDKEGKKPILPFTSYLGGLLQAYWV
SALLNNKKLPQAYWFDLKNMIFSSFEVPKKETCPVCGRNSRGRRLW
>Mature_366_residues
MDDTVILTLYERQILVEGIDIEFQQRLTEQVLGVPRIPAERLIVRLLFRSGLLGVSQDYSWDEMKVLSGINSYIFPSSET
TVIFENSSEMSVEYDNTNDRVTVKSPANISDIAAVILASKVFMAFLKKEGTKLSPDIIPDFGRFSIPENKKVMVVGAGGL
GSPVIEYLLRWGLKKLVVVDNDWVNVSNLHRQTLYFYEDIGKPKVKVLEKRISRFMQDVEIIGYETTFCGEMLSKEKPDV
VVSAVDNFRTRYEINYECYRRYVPFIDAGVSGYEGYVMRHTPEESCYRCFVGDDKKDKEGKKPILPFTSYLGGLLQAYWV
SALLNNKKLPQAYWFDLKNMIFSSFEVPKKETCPVCGRNSRGRRLW

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI4885649, Length=151, Percent_Identity=29.8013245033113, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI7657339, Length=142, Percent_Identity=33.8028169014084, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI38045944, Length=186, Percent_Identity=27.9569892473118, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI38045942, Length=186, Percent_Identity=27.9569892473118, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87082356, Length=217, Percent_Identity=33.6405529953917, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787048, Length=229, Percent_Identity=26.6375545851528, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17540406, Length=237, Percent_Identity=26.5822784810127, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI193203301, Length=145, Percent_Identity=32.4137931034483, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71984476, Length=172, Percent_Identity=26.7441860465116, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321965, Length=263, Percent_Identity=26.2357414448669, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320598, Length=146, Percent_Identity=28.0821917808219, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321903, Length=151, Percent_Identity=30.4635761589404, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325323, Length=157, Percent_Identity=28.6624203821656, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24660640, Length=150, Percent_Identity=34, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 41979; Mature: 41979

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDTVILTLYERQILVEGIDIEFQQRLTEQVLGVPRIPAERLIVRLLFRSGLLGVSQDYS
CCCCEEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCC
WDEMKVLSGINSYIFPSSETTVIFENSSEMSVEYDNTNDRVTVKSPANISDIAAVILASK
HHHHHHHHCCHHHCCCCCCEEEEEECCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHH
VFMAFLKKEGTKLSPDIIPDFGRFSIPENKKVMVVGAGGLGSPVIEYLLRWGLKKLVVVD
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEE
NDWVNVSNLHRQTLYFYEDIGKPKVKVLEKRISRFMQDVEIIGYETTFCGEMLSKEKPDV
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCE
VVSAVDNFRTRYEINYECYRRYVPFIDAGVSGYEGYVMRHTPEESCYRCFVGDDKKDKEG
EEEEHHCCCEEEEECHHHHHHHCCHHHCCCCCCCCEEEECCCHHHHEEEECCCCCCCCCC
KKPILPFTSYLGGLLQAYWVSALLNNKKLPQAYWFDLKNMIFSSFEVPKKETCPVCGRNS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
RGRRLW
CCCCCC
>Mature Secondary Structure
MDDTVILTLYERQILVEGIDIEFQQRLTEQVLGVPRIPAERLIVRLLFRSGLLGVSQDYS
CCCCEEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCC
WDEMKVLSGINSYIFPSSETTVIFENSSEMSVEYDNTNDRVTVKSPANISDIAAVILASK
HHHHHHHHCCHHHCCCCCCEEEEEECCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHH
VFMAFLKKEGTKLSPDIIPDFGRFSIPENKKVMVVGAGGLGSPVIEYLLRWGLKKLVVVD
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEE
NDWVNVSNLHRQTLYFYEDIGKPKVKVLEKRISRFMQDVEIIGYETTFCGEMLSKEKPDV
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCE
VVSAVDNFRTRYEINYECYRRYVPFIDAGVSGYEGYVMRHTPEESCYRCFVGDDKKDKEG
EEEEHHCCCEEEEECHHHHHHHCCHHHCCCCCCCCEEEECCCHHHHEEEECCCCCCCCCC
KKPILPFTSYLGGLLQAYWVSALLNNKKLPQAYWFDLKNMIFSSFEVPKKETCPVCGRNS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
RGRRLW
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]