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The map label for this gene is fmt

Identifier: 239617142

GI number: 239617142

Start: 797796

End: 798731

Strand: Direct

Name: fmt

Synonym: Kole_0746

Alternate gene names: 239617142

Gene position: 797796-798731 (Clockwise)

Preceding gene: 239617141

Following gene: 239617143

Centisome position: 34.65

GC content: 42.31

Gene sequence:

>936_bases
ATGAAAATAGTTTTTATGGGAACACCTGATTTTGCAGCGGAACATCTAAGAAAACTTGTTGAAAAAAAATACAATGTTGT
TGGTGTCTTCTCGCAACCAGATAAACCGAAAGGTAGAGGAAAAAAACTCATTCCTACACCGGTTAAACAGGTGGCCAGGG
AATATGGGATACCGGTATTCCAGCCAAAATCCGTAAACAAAGGCGAGGGATTCGAAGCCCTAAAAGAGCTCAAGCCGGAT
ATTATCATCACCGTTGCTTACGGAAAGTTACTCAAACAACAAGTTTTCGAGCTACCACCTCTCGGATGTTACAACGTTCA
TGCTTCATTGCTGCCTAAGTACAGAGGAGCAGCACCGATACAACGTGCTTTAGAGAATGGCGAGAAAGAAACAGGAATAA
CTATATTCAAGATAGATGAGGGTATGGATAGTGGCCCGATAGCGCTGCAAGAAAGAATTGAGATTTCTTCTGATGATAAC
TTCGGAACGCTGAAGAAAAAGCTATGCAATCTTGGCAAAAAACTGCTTATAGAATTTCTTAAAAAAATCTCTGCTGGCGA
GATTAAACTTACTCCCCAGGATCATTCCCAGGCAACCTACGCACCAAAGATAACTAAAGAGGATACCATTCTGATAGAAT
TTGACAACGGAGAACGAGTTTTTAACAAGATACGCGCCTATGATCCTGAACCCGGGGTTACCACCAGACTCGGAGAGCTC
AGAGTTAAGCTCTTCGGTGCGGGGATTTGTGATAATTGCTGCGTTGATGCCGAGCCAGGGCAAATAATCTCGATCAGCAA
GGATTCTATGGTAGTTGCCTGTAAAAAGGGTGCTGTTAAAATTTCCAAAATTCAGTTTCCCGGGAAAAAAGTGATAACTG
TCTGGCAAGCGAAATCTGGCAGATTGATAGAAGAAGGAATAAAACTAGGAGGTTGA

Upstream 100 bases:

>100_bases
ATCACCTGAATGGCATTCTTTTCATAGATTATCTATCTCCCGCAAAGAAAATGATGATAAGGCCAAAACTGAGGGAGATA
ATGAAGGGGAAAATTCTAAA

Downstream 100 bases:

>100_bases
AAAATGGCAAGGGCTTTGAAAATGTACCACATAAAAAATCTTGAAACACAGGAAGATGAAGAAAAAATCAAAGCGGTACT
CAAAGGTGTTTCCGGAATTA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKIVFMGTPDFAAEHLRKLVEKKYNVVGVFSQPDKPKGRGKKLIPTPVKQVAREYGIPVFQPKSVNKGEGFEALKELKPD
IIITVAYGKLLKQQVFELPPLGCYNVHASLLPKYRGAAPIQRALENGEKETGITIFKIDEGMDSGPIALQERIEISSDDN
FGTLKKKLCNLGKKLLIEFLKKISAGEIKLTPQDHSQATYAPKITKEDTILIEFDNGERVFNKIRAYDPEPGVTTRLGEL
RVKLFGAGICDNCCVDAEPGQIISISKDSMVVACKKGAVKISKIQFPGKKVITVWQAKSGRLIEEGIKLGG

Sequences:

>Translated_311_residues
MKIVFMGTPDFAAEHLRKLVEKKYNVVGVFSQPDKPKGRGKKLIPTPVKQVAREYGIPVFQPKSVNKGEGFEALKELKPD
IIITVAYGKLLKQQVFELPPLGCYNVHASLLPKYRGAAPIQRALENGEKETGITIFKIDEGMDSGPIALQERIEISSDDN
FGTLKKKLCNLGKKLLIEFLKKISAGEIKLTPQDHSQATYAPKITKEDTILIEFDNGERVFNKIRAYDPEPGVTTRLGEL
RVKLFGAGICDNCCVDAEPGQIISISKDSMVVACKKGAVKISKIQFPGKKVITVWQAKSGRLIEEGIKLGG
>Mature_311_residues
MKIVFMGTPDFAAEHLRKLVEKKYNVVGVFSQPDKPKGRGKKLIPTPVKQVAREYGIPVFQPKSVNKGEGFEALKELKPD
IIITVAYGKLLKQQVFELPPLGCYNVHASLLPKYRGAAPIQRALENGEKETGITIFKIDEGMDSGPIALQERIEISSDDN
FGTLKKKLCNLGKKLLIEFLKKISAGEIKLTPQDHSQATYAPKITKEDTILIEFDNGERVFNKIRAYDPEPGVTTRLGEL
RVKLFGAGICDNCCVDAEPGQIISISKDSMVVACKKGAVKISKIQFPGKKVITVWQAKSGRLIEEGIKLGG

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=310, Percent_Identity=29.0322580645161, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI21614513, Length=259, Percent_Identity=31.6602316602317, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI164663775, Length=236, Percent_Identity=31.7796610169492, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1789683, Length=293, Percent_Identity=43.0034129692833, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI1788589, Length=282, Percent_Identity=33.6879432624113, Blast_Score=167, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI133930964, Length=261, Percent_Identity=29.5019157088123, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319458, Length=359, Percent_Identity=26.1838440111421, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI45550868, Length=306, Percent_Identity=28.1045751633987, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28571984, Length=227, Percent_Identity=30.8370044052863, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24585660, Length=262, Percent_Identity=28.6259541984733, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24582400, Length=155, Percent_Identity=30.3225806451613, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_KOSOT (C5CG19)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002940464.1
- GeneID:   7969347
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_0746
- ProtClustDB:   CLSK2541091
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34280; Mature: 34280

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVFMGTPDFAAEHLRKLVEKKYNVVGVFSQPDKPKGRGKKLIPTPVKQVAREYGIPVF
CEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
QPKSVNKGEGFEALKELKPDIIITVAYGKLLKQQVFELPPLGCYNVHASLLPKYRGAAPI
CCCCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCEECCHHHHCCCCCCCHHH
QRALENGEKETGITIFKIDEGMDSGPIALQERIEISSDDNFGTLKKKLCNLGKKLLIEFL
HHHHHCCCCCCCEEEEEECCCCCCCCCEEHEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
KKISAGEIKLTPQDHSQATYAPKITKEDTILIEFDNGERVFNKIRAYDPEPGVTTRLGEL
HHCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCHHHHHE
RVKLFGAGICDNCCVDAEPGQIISISKDSMVVACKKGAVKISKIQFPGKKVITVWQAKSG
EEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEEEECCCCEEEEEEECCCC
RLIEEGIKLGG
CCHHHHHCCCC
>Mature Secondary Structure
MKIVFMGTPDFAAEHLRKLVEKKYNVVGVFSQPDKPKGRGKKLIPTPVKQVAREYGIPVF
CEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
QPKSVNKGEGFEALKELKPDIIITVAYGKLLKQQVFELPPLGCYNVHASLLPKYRGAAPI
CCCCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCEECCHHHHCCCCCCCHHH
QRALENGEKETGITIFKIDEGMDSGPIALQERIEISSDDNFGTLKKKLCNLGKKLLIEFL
HHHHHCCCCCCCEEEEEECCCCCCCCCEEHEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
KKISAGEIKLTPQDHSQATYAPKITKEDTILIEFDNGERVFNKIRAYDPEPGVTTRLGEL
HHCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCHHHHHE
RVKLFGAGICDNCCVDAEPGQIISISKDSMVVACKKGAVKISKIQFPGKKVITVWQAKSG
EEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEEEECCCCEEEEEEECCCC
RLIEEGIKLGG
CCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA