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The map label for this gene is def [H]

Identifier: 239617141

GI number: 239617141

Start: 797305

End: 797799

Strand: Direct

Name: def [H]

Synonym: Kole_0745

Alternate gene names: 239617141

Gene position: 797305-797799 (Clockwise)

Preceding gene: 239617140

Following gene: 239617142

Centisome position: 34.63

GC content: 41.21

Gene sequence:

>495_bases
ATGCATATTGTCTATATCGGAAATCCCGTATTGAGAGAAAAATCCGAGGCGATAAAAAAGTTTGACGATAATTTAAAAAC
TTTCGTCAAAGAGCTCACAAGCATTATGTACAAAGAAGATGGCGTTGGTTTGGCCGCGCCGCAAGTTGCTGTTTTAAAGC
GCATGTTTGTTTTCGACGACGGTTCAGGCCCTAAAGTAATAATCAATCCGGAGATACTCGAAAAATCAAAAGAACTCGTC
ATAATGGAAGAGGGATGCCTTAGCATCCCGGGGGTTTACGCTGACGTTGAAAGGCCTGAATGGGTTAAGATGAGATACCA
GGATGTTGACGGTAACGTTCACGAAGAGCTATTTGAAGGTTACGCTGGTAGAATTGTTCAGCATGAGTACGATCACCTGA
ATGGCATTCTTTTCATAGATTATCTATCTCCCGCAAAGAAAATGATGATAAGGCCAAAACTGAGGGAGATAATGAAGGGG
AAAATTCTAAAATGA

Upstream 100 bases:

>100_bases
TGTTCGGAGAAACTATGTTTCAATAACTCCCATATATGCTTTCATGACCAATCAAAACTATATGCCCAAGCTAAAGGAGG
AATTGGAAGGAGGCAATTGA

Downstream 100 bases:

>100_bases
AAATAGTTTTTATGGGAACACCTGATTTTGCAGCGGAACATCTAAGAAAACTTGTTGAAAAAAAATACAATGTTGTTGGT
GTCTTCTCGCAACCAGATAA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MHIVYIGNPVLREKSEAIKKFDDNLKTFVKELTSIMYKEDGVGLAAPQVAVLKRMFVFDDGSGPKVIINPEILEKSKELV
IMEEGCLSIPGVYADVERPEWVKMRYQDVDGNVHEELFEGYAGRIVQHEYDHLNGILFIDYLSPAKKMMIRPKLREIMKG
KILK

Sequences:

>Translated_164_residues
MHIVYIGNPVLREKSEAIKKFDDNLKTFVKELTSIMYKEDGVGLAAPQVAVLKRMFVFDDGSGPKVIINPEILEKSKELV
IMEEGCLSIPGVYADVERPEWVKMRYQDVDGNVHEELFEGYAGRIVQHEYDHLNGILFIDYLSPAKKMMIRPKLREIMKG
KILK
>Mature_164_residues
MHIVYIGNPVLREKSEAIKKFDDNLKTFVKELTSIMYKEDGVGLAAPQVAVLKRMFVFDDGSGPKVIINPEILEKSKELV
IMEEGCLSIPGVYADVERPEWVKMRYQDVDGNVHEELFEGYAGRIVQHEYDHLNGILFIDYLSPAKKMMIRPKLREIMKG
KILK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=161, Percent_Identity=29.8136645962733, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1789682, Length=160, Percent_Identity=41.25, Blast_Score=112, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24645728, Length=163, Percent_Identity=33.1288343558282, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24645726, Length=163, Percent_Identity=32.5153374233129, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18827; Mature: 18827

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIVYIGNPVLREKSEAIKKFDDNLKTFVKELTSIMYKEDGVGLAAPQVAVLKRMFVFDD
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEC
GSGPKVIINPEILEKSKELVIMEEGCLSIPGVYADVERPEWVKMRYQDVDGNVHEELFEG
CCCCEEEECHHHHCCCCCEEEECCCCCCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHH
YAGRIVQHEYDHLNGILFIDYLSPAKKMMIRPKLREIMKGKILK
HHHHHHHHHHHHCCCEEEEEECCHHHHHHHCHHHHHHHCCCCCC
>Mature Secondary Structure
MHIVYIGNPVLREKSEAIKKFDDNLKTFVKELTSIMYKEDGVGLAAPQVAVLKRMFVFDD
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEC
GSGPKVIINPEILEKSKELVIMEEGCLSIPGVYADVERPEWVKMRYQDVDGNVHEELFEG
CCCCEEEECHHHHCCCCCEEEECCCCCCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHH
YAGRIVQHEYDHLNGILFIDYLSPAKKMMIRPKLREIMKGKILK
HHHHHHHHHHHHCCCEEEEEECCHHHHHHHCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA