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The map label for this gene is glpK2 [H]

Identifier: 239616936

GI number: 239616936

Start: 585320

End: 586780

Strand: Direct

Name: glpK2 [H]

Synonym: Kole_0534

Alternate gene names: 239616936

Gene position: 585320-586780 (Clockwise)

Preceding gene: 239616935

Following gene: 239616937

Centisome position: 25.43

GC content: 43.19

Gene sequence:

>1461_bases
ATGGGGTACATCCTGGCAATTGATCAGGGGACCAGTAGTTCAAGGGCTATTATCTTTGATGAGGATCTCTATCAAGTTGC
TAGCGCACAAAAGCCTTTCAAACAAATTTATCCGAAACCTGGCTGGGTGGAGCATGATCCAAACGAGATAGTTCTCAGTG
TTATGTCTTGTGTGGAAAAAGCAATTCAAGACGCTAAGATCTCTTTTAAGGACATTGAGGCGATAGGCATAACCAACCAG
AGAGAAACGATCGTTGCCTGGGATGCCGAAACGGGCAAAGCTCTCTACAATGCCATTGTCTGGCAATGTAGAAGGACAGC
GGATAGGGCTAAAGAACTGGAAAGACAATATCGGAATTTTATACATTCAAAAACTGGTTTGCTTCCTGACCCTTATTTTT
CGGCAACAAAGATGGAATGGATGCTAAGAAATGTTCCAGAAGTTAGAAAAGCCGCGAAAAAAGGAACTTTAAGATTTGGT
ACCATAGATTCCTGGTTGATTTGGAATCTAACACCTGACAGACAACATGTAACCGATCACTCAAACGCTTCCAGAACCAT
GCTTTATAACTTGAACGAGTTGAAATGGGACGAAGAATTGCTCGAGTTTTTCGGTATCGAAAAAGAATTTTTGCCAAAGG
TTGTTGATTCTTCAAAACTGCTGATACGTTCTGTATATGGCCCGGTAATATCCGGCATTGCCGGAGATCAACAAGCATCT
CTTTTCGGGCAAACCGCTTTTAACGTAGGTGACGTAAAATGTACCTACGGAACCGGTTCTTTCATCCTGATGAACACTGG
AGATAAACCAAGATTCTCGAGAGAAGGGCTTTTAACCACCGTAGGTTGGAAGATCAACGATGAAATTGTCTATGCAATCG
AGGGCTCAATATTTACAACAGGTGCCCTTATAAACTGGCTGAAAGATGGTTTGCAGATAATCTCCACTCCCGAAGAAACG
GAGTTATTGGCAACCAGTGTTGAAGATAATGGCGGTGTCTATTTTTCTGGTGCCCTTGCCGGTCTCGGTGCACCATACTG
GGATTCTAAAGCCCGCGGCTTGATCATAGGTTTAACCAGAGGAGCAACCCGTGCACATATAGTCAGAGCTGTGTTAGAAT
ACATTGCTTTTAGAACAAAAGAAATACTGGAAATAATGGAAAGGGATACAGGAATAAAGGTCAATCGACTCCTTGTTGAT
GGTGGCGTTACGAGGAACAACTTTTTGATGGAACTTCAGTCTAAAGTATTAGGTGTAACTGTCGATAGACCAATAGTCAA
GGAGACCACAGCACTTGGAGCAGCCATGCTTGCCGGGCTCGCAGTTGGTTTATGGGATAAAGAAACCCTCGCGGGAATAC
GGAAAAGCGAAGTCGTTTTTACCCCAACGAGAAAAGAATTGGAGGATGAATACAAAAAGTGGAAAGAAGCGATAAAACGT
TCTATGAACTGGGCGGAATGA

Upstream 100 bases:

>100_bases
ACGGAAAAACATGAAGAAAACCCTGAAGAAACAGAAGATGGGGAAAACGGAGATATTACGCCGACCACAAAATAAATAAA
AAAAGTTAGGGGGGATTTTC

Downstream 100 bases:

>100_bases
ATGAAAAAGCTGTAAGACACCTGGCTTTTGAGATTGCTAAAAGGCTTGAAGGTGGTGAAATTCTTCTCTTAAAGGGTGAT
TTAGGAACAGGAAAAACTAC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase 2; Glycerokinase 2; GK 2 [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MGYILAIDQGTSSSRAIIFDEDLYQVASAQKPFKQIYPKPGWVEHDPNEIVLSVMSCVEKAIQDAKISFKDIEAIGITNQ
RETIVAWDAETGKALYNAIVWQCRRTADRAKELERQYRNFIHSKTGLLPDPYFSATKMEWMLRNVPEVRKAAKKGTLRFG
TIDSWLIWNLTPDRQHVTDHSNASRTMLYNLNELKWDEELLEFFGIEKEFLPKVVDSSKLLIRSVYGPVISGIAGDQQAS
LFGQTAFNVGDVKCTYGTGSFILMNTGDKPRFSREGLLTTVGWKINDEIVYAIEGSIFTTGALINWLKDGLQIISTPEET
ELLATSVEDNGGVYFSGALAGLGAPYWDSKARGLIIGLTRGATRAHIVRAVLEYIAFRTKEILEIMERDTGIKVNRLLVD
GGVTRNNFLMELQSKVLGVTVDRPIVKETTALGAAMLAGLAVGLWDKETLAGIRKSEVVFTPTRKELEDEYKKWKEAIKR
SMNWAE

Sequences:

>Translated_486_residues
MGYILAIDQGTSSSRAIIFDEDLYQVASAQKPFKQIYPKPGWVEHDPNEIVLSVMSCVEKAIQDAKISFKDIEAIGITNQ
RETIVAWDAETGKALYNAIVWQCRRTADRAKELERQYRNFIHSKTGLLPDPYFSATKMEWMLRNVPEVRKAAKKGTLRFG
TIDSWLIWNLTPDRQHVTDHSNASRTMLYNLNELKWDEELLEFFGIEKEFLPKVVDSSKLLIRSVYGPVISGIAGDQQAS
LFGQTAFNVGDVKCTYGTGSFILMNTGDKPRFSREGLLTTVGWKINDEIVYAIEGSIFTTGALINWLKDGLQIISTPEET
ELLATSVEDNGGVYFSGALAGLGAPYWDSKARGLIIGLTRGATRAHIVRAVLEYIAFRTKEILEIMERDTGIKVNRLLVD
GGVTRNNFLMELQSKVLGVTVDRPIVKETTALGAAMLAGLAVGLWDKETLAGIRKSEVVFTPTRKELEDEYKKWKEAIKR
SMNWAE
>Mature_485_residues
GYILAIDQGTSSSRAIIFDEDLYQVASAQKPFKQIYPKPGWVEHDPNEIVLSVMSCVEKAIQDAKISFKDIEAIGITNQR
ETIVAWDAETGKALYNAIVWQCRRTADRAKELERQYRNFIHSKTGLLPDPYFSATKMEWMLRNVPEVRKAAKKGTLRFGT
IDSWLIWNLTPDRQHVTDHSNASRTMLYNLNELKWDEELLEFFGIEKEFLPKVVDSSKLLIRSVYGPVISGIAGDQQASL
FGQTAFNVGDVKCTYGTGSFILMNTGDKPRFSREGLLTTVGWKINDEIVYAIEGSIFTTGALINWLKDGLQIISTPEETE
LLATSVEDNGGVYFSGALAGLGAPYWDSKARGLIIGLTRGATRAHIVRAVLEYIAFRTKEILEIMERDTGIKVNRLLVDG
GVTRNNFLMELQSKVLGVTVDRPIVKETTALGAAMLAGLAVGLWDKETLAGIRKSEVVFTPTRKELEDEYKKWKEAIKRS
MNWAE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=499, Percent_Identity=45.8917835671343, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI4504007, Length=499, Percent_Identity=45.8917835671343, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI42794763, Length=505, Percent_Identity=45.3465346534653, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI41393575, Length=501, Percent_Identity=44.9101796407186, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI88196792, Length=513, Percent_Identity=36.2573099415205, Blast_Score=327, Evalue=1e-89,
Organism=Escherichia coli, GI1790361, Length=496, Percent_Identity=50.8064516129032, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1787789, Length=529, Percent_Identity=24.7637051039698, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1790005, Length=507, Percent_Identity=24.4575936883629, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789987, Length=451, Percent_Identity=22.6164079822616, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1789168, Length=491, Percent_Identity=22.6069246435845, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=504, Percent_Identity=42.2619047619048, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71986140, Length=523, Percent_Identity=27.7246653919694, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI71986147, Length=260, Percent_Identity=36.1538461538462, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321755, Length=551, Percent_Identity=34.8457350272232, Blast_Score=285, Evalue=9e-78,
Organism=Drosophila melanogaster, GI24655555, Length=503, Percent_Identity=42.3459244532803, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655560, Length=503, Percent_Identity=42.3459244532803, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655570, Length=503, Percent_Identity=42.3459244532803, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655574, Length=503, Percent_Identity=42.3459244532803, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655565, Length=503, Percent_Identity=42.3459244532803, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17864214, Length=506, Percent_Identity=42.0948616600791, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24654573, Length=458, Percent_Identity=42.5764192139738, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI19922058, Length=526, Percent_Identity=32.1292775665399, Blast_Score=254, Evalue=9e-68,
Organism=Drosophila melanogaster, GI21357867, Length=528, Percent_Identity=29.7348484848485, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24656367, Length=513, Percent_Identity=30.6042884990253, Blast_Score=244, Evalue=9e-65,
Organism=Drosophila melanogaster, GI24652871, Length=493, Percent_Identity=32.657200811359, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24656372, Length=369, Percent_Identity=31.9783197831978, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI45552909, Length=310, Percent_Identity=30, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54494; Mature: 54363

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYILAIDQGTSSSRAIIFDEDLYQVASAQKPFKQIYPKPGWVEHDPNEIVLSVMSCVEK
CCEEEEEECCCCCCEEEEEEHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHH
AIQDAKISFKDIEAIGITNQRETIVAWDAETGKALYNAIVWQCRRTADRAKELERQYRNF
HHHHCCCCHHHHHEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHSKTGLLPDPYFSATKMEWMLRNVPEVRKAAKKGTLRFGTIDSWLIWNLTPDRQHVTDH
HHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCEEEECCCCEEEEECCCCHHHCCCC
SNASRTMLYNLNELKWDEELLEFFGIEKEFLPKVVDSSKLLIRSVYGPVISGIAGDQQAS
CCCCCEEEEECHHCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
LFGQTAFNVGDVKCTYGTGSFILMNTGDKPRFSREGLLTTVGWKINDEIVYAIEGSIFTT
HHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEECEEECCCEEEEEECCEEHH
GALINWLKDGLQIISTPEETELLATSVEDNGGVYFSGALAGLGAPYWDSKARGLIIGLTR
HHHHHHHHHHHHEECCCCHHHHEEEEECCCCCEEEECHHHHCCCCCCCCCCCEEEEEECC
GATRAHIVRAVLEYIAFRTKEILEIMERDTGIKVNRLLVDGGVTRNNFLMELQSKVLGVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHEEE
VDRPIVKETTALGAAMLAGLAVGLWDKETLAGIRKSEVVFTPTRKELEDEYKKWKEAIKR
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHH
SMNWAE
CCCCCC
>Mature Secondary Structure 
GYILAIDQGTSSSRAIIFDEDLYQVASAQKPFKQIYPKPGWVEHDPNEIVLSVMSCVEK
CEEEEEECCCCCCEEEEEEHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHH
AIQDAKISFKDIEAIGITNQRETIVAWDAETGKALYNAIVWQCRRTADRAKELERQYRNF
HHHHCCCCHHHHHEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHSKTGLLPDPYFSATKMEWMLRNVPEVRKAAKKGTLRFGTIDSWLIWNLTPDRQHVTDH
HHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCEEEECCCCEEEEECCCCHHHCCCC
SNASRTMLYNLNELKWDEELLEFFGIEKEFLPKVVDSSKLLIRSVYGPVISGIAGDQQAS
CCCCCEEEEECHHCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
LFGQTAFNVGDVKCTYGTGSFILMNTGDKPRFSREGLLTTVGWKINDEIVYAIEGSIFTT
HHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEECEEECCCEEEEEECCEEHH
GALINWLKDGLQIISTPEETELLATSVEDNGGVYFSGALAGLGAPYWDSKARGLIIGLTR
HHHHHHHHHHHHEECCCCHHHHEEEEECCCCCEEEECHHHHCCCCCCCCCCCEEEEEECC
GATRAHIVRAVLEYIAFRTKEILEIMERDTGIKVNRLLVDGGVTRNNFLMELQSKVLGVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHEEE
VDRPIVKETTALGAAMLAGLAVGLWDKETLAGIRKSEVVFTPTRKELEDEYKKWKEAIKR
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHH
SMNWAE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]