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The map label for this gene is istB [H]

Identifier: 239616929

GI number: 239616929

Start: 575891

End: 576688

Strand: Direct

Name: istB [H]

Synonym: Kole_0526

Alternate gene names: 239616929

Gene position: 575891-576688 (Clockwise)

Preceding gene: 239616928

Following gene: 239616935

Centisome position: 25.02

GC content: 41.48

Gene sequence:

>798_bases
ATGAGCTACGAAAAAGTACACAGCTTGTTGAAAGAACTGAAACTGGAAGGAATCTCCAGGGTATTGGATTCTTCTCTCCA
AAACTGGAGCAAAGGAGATAAAGACGTTCTCGAATTGATTGAAGAACTACTAATAGCCCAGAAGAAAGAAAACGAGAACA
AGAAATACATAACAGCTCTCAGATACAGCGGATTACCTTTCCACAAAACCCTGGAAGAATTCGATTTCAGTTTCCAACCC
AGCATAGACAGAAAACAGGTAATGGAACTGAAGACCCTGAGATTCATGTACGAAAAAGAGAACGTGGTATTTCTTGGACC
TCCTGGAGTAGGAAAAACACACCTGGCGGTAGGTCTTGGAATGGAAGCCCTAAGGGAAGGAAAGAAAATATACTTCGTTA
ATGCAATAACGTTGGTGGATCGATTAAAGAAAGCCTTTGTTGAGAGACATCTGGAAAAAACCATTAGATTCTACAAATCC
CTTGACCTACTAATAATAGATGAACTGGGATACCTGCCGCTGGATACAGAGGGAGCGAAGCTGTTTTTTGAACTGGTGAG
CCAGAAATACGAGCAGGGAAGCATAATCCTGACATCCAACAGAAGCTTTACCGAATGGGACAAGATATTTGAAGACGAAG
TATTAGCAACAGCGGTATTGGACAGACTCTTACACCATTGTACGATAGTCAATATTCGTGGAAAAAGTTACAGACTGAAA
GAAAAACAGAAAACGGGGCTTGTTGGTATACAGACGACGATTGGTAGAACCGACAAACTACGCAAATCTGAGCGATGA

Upstream 100 bases:

>100_bases
AGATATACAGCAACGATGAACTCATTACAGTACATCCATTGAAAGACCAGGTAGAAAAAAGGTCTTTGAGAGAATACCAG
GCTCTTGTGGGTGATGCGGT

Downstream 100 bases:

>100_bases
AATTACGCAATTTTGATCGATGTTTTTTTGCATTTTTGGTTGATGTTGACAGCTGGGATATGTCAGGAAATACTGGCCGA
AACAGCACCATCCTGTTAGG

Product: IstB domain protein ATP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSYEKVHSLLKELKLEGISRVLDSSLQNWSKGDKDVLELIEELLIAQKKENENKKYITALRYSGLPFHKTLEEFDFSFQP
SIDRKQVMELKTLRFMYEKENVVFLGPPGVGKTHLAVGLGMEALREGKKIYFVNAITLVDRLKKAFVERHLEKTIRFYKS
LDLLIIDELGYLPLDTEGAKLFFELVSQKYEQGSIILTSNRSFTEWDKIFEDEVLATAVLDRLLHHCTIVNIRGKSYRLK
EKQKTGLVGIQTTIGRTDKLRKSER

Sequences:

>Translated_265_residues
MSYEKVHSLLKELKLEGISRVLDSSLQNWSKGDKDVLELIEELLIAQKKENENKKYITALRYSGLPFHKTLEEFDFSFQP
SIDRKQVMELKTLRFMYEKENVVFLGPPGVGKTHLAVGLGMEALREGKKIYFVNAITLVDRLKKAFVERHLEKTIRFYKS
LDLLIIDELGYLPLDTEGAKLFFELVSQKYEQGSIILTSNRSFTEWDKIFEDEVLATAVLDRLLHHCTIVNIRGKSYRLK
EKQKTGLVGIQTTIGRTDKLRKSER
>Mature_264_residues
SYEKVHSLLKELKLEGISRVLDSSLQNWSKGDKDVLELIEELLIAQKKENENKKYITALRYSGLPFHKTLEEFDFSFQPS
IDRKQVMELKTLRFMYEKENVVFLGPPGVGKTHLAVGLGMEALREGKKIYFVNAITLVDRLKKAFVERHLEKTIRFYKSL
DLLIIDELGYLPLDTEGAKLFFELVSQKYEQGSIILTSNRSFTEWDKIFEDEVLATAVLDRLLHHCTIVNIRGKSYRLKE
KQKTGLVGIQTTIGRTDKLRKSER

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 30718; Mature: 30587

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYEKVHSLLKELKLEGISRVLDSSLQNWSKGDKDVLELIEELLIAQKKENENKKYITAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
RYSGLPFHKTLEEFDFSFQPSIDRKQVMELKTLRFMYEKENVVFLGPPGVGKTHLAVGLG
ECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCC
MEALREGKKIYFVNAITLVDRLKKAFVERHLEKTIRFYKSLDLLIIDELGYLPLDTEGAK
HHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHH
LFFELVSQKYEQGSIILTSNRSFTEWDKIFEDEVLATAVLDRLLHHCTIVNIRGKSYRLK
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEECC
EKQKTGLVGIQTTIGRTDKLRKSER
HHHCCCCEEEHHHHCCCHHHHCCCC
>Mature Secondary Structure 
SYEKVHSLLKELKLEGISRVLDSSLQNWSKGDKDVLELIEELLIAQKKENENKKYITAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
RYSGLPFHKTLEEFDFSFQPSIDRKQVMELKTLRFMYEKENVVFLGPPGVGKTHLAVGLG
ECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCC
MEALREGKKIYFVNAITLVDRLKKAFVERHLEKTIRFYKSLDLLIIDELGYLPLDTEGAK
HHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHH
LFFELVSQKYEQGSIILTSNRSFTEWDKIFEDEVLATAVLDRLLHHCTIVNIRGKSYRLK
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEECC
EKQKTGLVGIQTTIGRTDKLRKSER
HHHCCCCEEEHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]