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The map label for this gene is maeB [C]

Identifier: 239616892

GI number: 239616892

Start: 527290

End: 528444

Strand: Direct

Name: maeB [C]

Synonym: Kole_0487

Alternate gene names: 239616892

Gene position: 527290-528444 (Clockwise)

Preceding gene: 239616891

Following gene: 239616893

Centisome position: 22.9

GC content: 46.15

Gene sequence:

>1155_bases
ATGGATCTCAAAGAAAAAGCACTTAAACAGCATGAATTATGGCAGGGAAAACTCGAGGTTATTTCCAAAGCGAAAGTAGA
AACCTCCGAAGACCTCTCAATCGCCTACACCCCTGGAGTAGCCGAACCTTGCAGAAAAATAGCAGAAGATCCCGAAAAAG
TGTATAAGTATACAATGAAAGGCAACACCGTTGCCGTAGTCACAGACGGAACGGCTGTTCTCGGTCTGGGAGACATCGGC
CCCGAAGCCGCTTTGCCTGTGATGGAAGGAAAAGCGGTTCTCTTCAAAGAATTTGCCGGCATAGATGCTTTCCCGATATG
CCTGGACACAAAAGATGTGGATGAAATAGTAAAAGCCGTTAAATACATTGCTCCCGGCTTTGGTGGAATAAACCTGGAAG
ACATTTCCGCTCCACGATGCTTTGAAATCGAAGAAAGATTGAAAGCCGAATTAAATATCCCGGTTTTCCACGACGATCAG
CACGGAACAGCTATAGTCGTGTTAGCCGGAATCATAAATTCTCTGAAGATCGTCCAGAAAAAGCCAAATGAAATGAAAGT
AGTGATCAACGGGGCTGGCGCTGCCGGAATAGCGATCACAAAACTCCTGATAAAATTCGGCTTTAAGCACATAATCCTCT
GCGATAAACCCGGAGCCATATATAGGGGAGCCGATTGGACAAATCCTGCCCAGACAAAAATCGCCGAAATCACCAACCCG
GACAACGTAAAGGGAACTCTGAAAGAAGTAATAAAGGGTGCAGACCTGTTCATAGGGGTTTCAGCACCAAACATTGTCAC
AGAAGAAATGATAGCTTCGATGAACAAAGACGCCATCGTTTTTGCTATGGCCAACCCTGTTCCGGAAATAATGCCGGATA
AAGCAAAAGCGGGTGGTGCAAGGATAGTTGGAACAGGCCGCTCAGATTTCCCAAACCAGGTAAACAACGTCCTCGCATTC
CCGGGAATATTCAAGGGGGCTCTAAAATGCCGAAAACAAATAACGGATAAAATGAAAATCGCCGCGGCTTACGCCATAGC
CGGCATGATACCGGAATCCGAATTAAACGAAGAAAACATACTCCCAAAACCCTTCCAGCCTGGAATAGCAGATGCCGTAG
CCGAAGCAGTCATAAAAGCAGCGGAAGAGGAATAA

Upstream 100 bases:

>100_bases
CAGAGAACAACTCGTTCAATTAAGAGATGAATTCAATTTAGACCAGTATAAATTCCCCTGGGAATGATTATAAAAACCAG
AAACATGCGGAGGGATAAAA

Downstream 100 bases:

>100_bases
CAACAAGGAGGTTTACAGGTGAGGATCAAAGTAATTCTCAAAGAAGCCAAAAACAAGGCTTTCAAAAGAGATCATTACCA
TCAGCTCGCTGGCATGATTT

Product: Malate dehydrogenase (oxaloacetate-decarboxylating)

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MDLKEKALKQHELWQGKLEVISKAKVETSEDLSIAYTPGVAEPCRKIAEDPEKVYKYTMKGNTVAVVTDGTAVLGLGDIG
PEAALPVMEGKAVLFKEFAGIDAFPICLDTKDVDEIVKAVKYIAPGFGGINLEDISAPRCFEIEERLKAELNIPVFHDDQ
HGTAIVVLAGIINSLKIVQKKPNEMKVVINGAGAAGIAITKLLIKFGFKHIILCDKPGAIYRGADWTNPAQTKIAEITNP
DNVKGTLKEVIKGADLFIGVSAPNIVTEEMIASMNKDAIVFAMANPVPEIMPDKAKAGGARIVGTGRSDFPNQVNNVLAF
PGIFKGALKCRKQITDKMKIAAAYAIAGMIPESELNEENILPKPFQPGIADAVAEAVIKAAEEE

Sequences:

>Translated_384_residues
MDLKEKALKQHELWQGKLEVISKAKVETSEDLSIAYTPGVAEPCRKIAEDPEKVYKYTMKGNTVAVVTDGTAVLGLGDIG
PEAALPVMEGKAVLFKEFAGIDAFPICLDTKDVDEIVKAVKYIAPGFGGINLEDISAPRCFEIEERLKAELNIPVFHDDQ
HGTAIVVLAGIINSLKIVQKKPNEMKVVINGAGAAGIAITKLLIKFGFKHIILCDKPGAIYRGADWTNPAQTKIAEITNP
DNVKGTLKEVIKGADLFIGVSAPNIVTEEMIASMNKDAIVFAMANPVPEIMPDKAKAGGARIVGTGRSDFPNQVNNVLAF
PGIFKGALKCRKQITDKMKIAAAYAIAGMIPESELNEENILPKPFQPGIADAVAEAVIKAAEEE
>Mature_384_residues
MDLKEKALKQHELWQGKLEVISKAKVETSEDLSIAYTPGVAEPCRKIAEDPEKVYKYTMKGNTVAVVTDGTAVLGLGDIG
PEAALPVMEGKAVLFKEFAGIDAFPICLDTKDVDEIVKAVKYIAPGFGGINLEDISAPRCFEIEERLKAELNIPVFHDDQ
HGTAIVVLAGIINSLKIVQKKPNEMKVVINGAGAAGIAITKLLIKFGFKHIILCDKPGAIYRGADWTNPAQTKIAEITNP
DNVKGTLKEVIKGADLFIGVSAPNIVTEEMIASMNKDAIVFAMANPVPEIMPDKAKAGGARIVGTGRSDFPNQVNNVLAF
PGIFKGALKCRKQITDKMKIAAAYAIAGMIPESELNEENILPKPFQPGIADAVAEAVIKAAEEE

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=434, Percent_Identity=26.2672811059908, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4505145, Length=363, Percent_Identity=25.8953168044077, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI62420882, Length=363, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI62420880, Length=363, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI239049447, Length=363, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI270265879, Length=373, Percent_Identity=25.201072386059, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788806, Length=401, Percent_Identity=49.6259351620948, Blast_Score=355, Evalue=3e-99,
Organism=Saccharomyces cerevisiae, GI6322823, Length=393, Percent_Identity=27.4809160305344, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281362674, Length=436, Percent_Identity=25.2293577981651, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356279, Length=436, Percent_Identity=25.2293577981651, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281362672, Length=436, Percent_Identity=25.2293577981651, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI78707242, Length=355, Percent_Identity=26.1971830985915, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI78707238, Length=355, Percent_Identity=26.1971830985915, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI78707240, Length=358, Percent_Identity=26.536312849162, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 41186; Mature: 41186

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKEKALKQHELWQGKLEVISKAKVETSEDLSIAYTPGVAEPCRKIAEDPEKVYKYTMK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHCCHHHHHEEEEC
GNTVAVVTDGTAVLGLGDIGPEAALPVMEGKAVLFKEFAGIDAFPICLDTKDVDEIVKAV
CCEEEEEECCEEEEEECCCCCCCCCCHHCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHH
KYIAPGFGGINLEDISAPRCFEIEERLKAELNIPVFHDDQHGTAIVVLAGIINSLKIVQK
HHHCCCCCCCCHHCCCCCCCCCHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHHC
KPNEMKVVINGAGAAGIAITKLLIKFGFKHIILCDKPGAIYRGADWTNPAQTKIAEITNP
CCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCHHHHHHEECCCC
DNVKGTLKEVIKGADLFIGVSAPNIVTEEMIASMNKDAIVFAMANPVPEIMPDKAKAGGA
CCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCE
RIVGTGRSDFPNQVNNVLAFPGIFKGALKCRKQITDKMKIAAAYAIAGMIPESELNEENI
EEEECCCCCCCHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LPKPFQPGIADAVAEAVIKAAEEE
CCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLKEKALKQHELWQGKLEVISKAKVETSEDLSIAYTPGVAEPCRKIAEDPEKVYKYTMK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHCCHHHHHEEEEC
GNTVAVVTDGTAVLGLGDIGPEAALPVMEGKAVLFKEFAGIDAFPICLDTKDVDEIVKAV
CCEEEEEECCEEEEEECCCCCCCCCCHHCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHH
KYIAPGFGGINLEDISAPRCFEIEERLKAELNIPVFHDDQHGTAIVVLAGIINSLKIVQK
HHHCCCCCCCCHHCCCCCCCCCHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHHC
KPNEMKVVINGAGAAGIAITKLLIKFGFKHIILCDKPGAIYRGADWTNPAQTKIAEITNP
CCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCHHHHHHEECCCC
DNVKGTLKEVIKGADLFIGVSAPNIVTEEMIASMNKDAIVFAMANPVPEIMPDKAKAGGA
CCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCE
RIVGTGRSDFPNQVNNVLAFPGIFKGALKCRKQITDKMKIAAAYAIAGMIPESELNEENI
EEEECCCCCCCHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LPKPFQPGIADAVAEAVIKAAEEE
CCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]