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The map label for this gene is 239616881

Identifier: 239616881

GI number: 239616881

Start: 510307

End: 511260

Strand: Direct

Name: 239616881

Synonym: Kole_0476

Alternate gene names: NA

Gene position: 510307-511260 (Clockwise)

Preceding gene: 239616880

Following gene: 239616882

Centisome position: 22.17

GC content: 45.6

Gene sequence:

>954_bases
ATGATCAGAATTTCCGTTGGAACAGCTAAAGTAATGGGCCTAACAGACTTACAGATAGATGTTTTGCCCACCACCGGTTA
CTTCATGATAGGCCAGAGTTGCATTTATGATTGCGCTTTTTGTTCTCAGTCACGAACCAGCCATACCCGTTCCGATCAAC
TTTCCCGCGTGACATGGCCGGAATACGACTTGGAACAGGTTATGAAGCATTTATCTACTACAACTGATAGAGAGGTAATC
CGTAGAATTTGCTTGCAGGTCGTTCACCAGCCGGGAATAAAAGACACCGTCCAGAAGATTTTGCATCGCCTTTCTCAAAG
TTCTGAGCTTCCCATTTGCGTTTGCATGCGGGTAAATCATGTAAAAGAAGCAGCAGAATTAATAGAATCAGGAGCAGAAC
GTGTCTGTATAGCTTTAGACGCCGCAAATCCACGCGTTTATGAGCAAATCAAAAACGGTTCCTGGCACAATAGAATGAAA
TTAATTGAAGAAGCAGCCGCAATGTTTCCAGGGCACATCAGCACTCACCTGATCGTCGGTCTGGGAGAAACAGAAAAAGA
AATGGTTGAAACCATCCAGCATATGTACCAGATCGGTGTCACCGTTGGCCTGTTTGCGTTCACTCCCATCAGAGGAACAC
GCATGTCTAAGAATCCATCCCCGCCACTCGATCAATACCGCCGAATACAAGTAGCACACTGGCTCATTAGACACGGCATC
TGTGATGGCAGCAACTTTTCCTATAAAAATGGTTCAATTACAGGCTTTGGACTCAGCAGCAAAGAACTCCTGAAACATCT
AGCTGACGGCGAAGCATTTAGAACGAGCGGATGCCCCGATTGCAACCGCCCCTATTATAATGAAAGACCGGGCGGTCCGA
TGTACAACTACCCACGCCCTTTAACACCTGACGAAATAACAAAAGCAATAGCCGAACTGAAAATAAAGCTTTAA

Upstream 100 bases:

>100_bases
GGTGCGGTATCAACAAGCTGGTTACGCCAGCACCTCAGGCAAAAGAAGAAGCCATTAACTTAGGGCTGACCATTAAGCAT
GGAGAGGAGTGCTGTGCGTT

Downstream 100 bases:

>100_bases
GCAAGAGAGGGAAAAGAGATATGGATAACGAATGGAGATTTATACAGACAGGTTTTCAAGATGGTGCCATGAACATGGCT
ATTGACGAAGCAATACTTAT

Product: Radical SAM domain protein

Products: biotin

Alternate protein names: Radical SAM Domain-Containing Protein; Biotin Synthase Related Protein; Biotin Synthase; Biotin Synthase-Related Protein; Biotin Synthase-Related; Radical SAM Superfamily Protein; Radical SAM Family Protein

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MIRISVGTAKVMGLTDLQIDVLPTTGYFMIGQSCIYDCAFCSQSRTSHTRSDQLSRVTWPEYDLEQVMKHLSTTTDREVI
RRICLQVVHQPGIKDTVQKILHRLSQSSELPICVCMRVNHVKEAAELIESGAERVCIALDAANPRVYEQIKNGSWHNRMK
LIEEAAAMFPGHISTHLIVGLGETEKEMVETIQHMYQIGVTVGLFAFTPIRGTRMSKNPSPPLDQYRRIQVAHWLIRHGI
CDGSNFSYKNGSITGFGLSSKELLKHLADGEAFRTSGCPDCNRPYYNERPGGPMYNYPRPLTPDEITKAIAELKIKL

Sequences:

>Translated_317_residues
MIRISVGTAKVMGLTDLQIDVLPTTGYFMIGQSCIYDCAFCSQSRTSHTRSDQLSRVTWPEYDLEQVMKHLSTTTDREVI
RRICLQVVHQPGIKDTVQKILHRLSQSSELPICVCMRVNHVKEAAELIESGAERVCIALDAANPRVYEQIKNGSWHNRMK
LIEEAAAMFPGHISTHLIVGLGETEKEMVETIQHMYQIGVTVGLFAFTPIRGTRMSKNPSPPLDQYRRIQVAHWLIRHGI
CDGSNFSYKNGSITGFGLSSKELLKHLADGEAFRTSGCPDCNRPYYNERPGGPMYNYPRPLTPDEITKAIAELKIKL
>Mature_317_residues
MIRISVGTAKVMGLTDLQIDVLPTTGYFMIGQSCIYDCAFCSQSRTSHTRSDQLSRVTWPEYDLEQVMKHLSTTTDREVI
RRICLQVVHQPGIKDTVQKILHRLSQSSELPICVCMRVNHVKEAAELIESGAERVCIALDAANPRVYEQIKNGSWHNRMK
LIEEAAAMFPGHISTHLIVGLGETEKEMVETIQHMYQIGVTVGLFAFTPIRGTRMSKNPSPPLDQYRRIQVAHWLIRHGI
CDGSNFSYKNGSITGFGLSSKELLKHLADGEAFRTSGCPDCNRPYYNERPGGPMYNYPRPLTPDEITKAIAELKIKL

Specific function: Unknown

COG id: COG2516

COG function: function code R; Biotin synthase-related enzyme

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.8.1.6

Molecular weight: Translated: 35694; Mature: 35694

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRISVGTAKVMGLTDLQIDVLPTTGYFMIGQSCIYDCAFCSQSRTSHTRSDQLSRVTWP
CEEEEECCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCC
EYDLEQVMKHLSTTTDREVIRRICLQVVHQPGIKDTVQKILHRLSQSSELPICVCMRVNH
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHH
VKEAAELIESGAERVCIALDAANPRVYEQIKNGSWHNRMKLIEEAAAMFPGHISTHLIVG
HHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
LGETEKEMVETIQHMYQIGVTVGLFAFTPIRGTRMSKNPSPPLDQYRRIQVAHWLIRHGI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
CDGSNFSYKNGSITGFGLSSKELLKHLADGEAFRTSGCPDCNRPYYNERPGGPMYNYPRP
CCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LTPDEITKAIAELKIKL
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRISVGTAKVMGLTDLQIDVLPTTGYFMIGQSCIYDCAFCSQSRTSHTRSDQLSRVTWP
CEEEEECCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCC
EYDLEQVMKHLSTTTDREVIRRICLQVVHQPGIKDTVQKILHRLSQSSELPICVCMRVNH
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHH
VKEAAELIESGAERVCIALDAANPRVYEQIKNGSWHNRMKLIEEAAAMFPGHISTHLIVG
HHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
LGETEKEMVETIQHMYQIGVTVGLFAFTPIRGTRMSKNPSPPLDQYRRIQVAHWLIRHGI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
CDGSNFSYKNGSITGFGLSSKELLKHLADGEAFRTSGCPDCNRPYYNERPGGPMYNYPRP
CCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LTPDEITKAIAELKIKL
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA