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The map label for this gene is hisS [H]

Identifier: 239616783

GI number: 239616783

Start: 393461

End: 394720

Strand: Direct

Name: hisS [H]

Synonym: Kole_0376

Alternate gene names: 239616783

Gene position: 393461-394720 (Clockwise)

Preceding gene: 239616782

Following gene: 239616784

Centisome position: 17.09

GC content: 40.79

Gene sequence:

>1260_bases
ATGATAACCAGAATAAAGGGAACACAGGATGTGTTTTTCGAAGAGATAAAATACTGGCACTACATCGAAAATACTATCCG
AAAGATAACAAAGCTGTACGCATTTGAAGAAATCCGCACACCCATTTTTGAACATACAGAGCTCTTCAAACGAAGTGTTG
GTGAATCCACCGATGTTGTACAAAAGGAGATGTACACCTTTAATGATAAAGGTGGGCGTTCTCTCACTCTGAGACCGGAA
GGTACAGCAAGCGTGGCAAGGGCTTACGTTGAAAATGGTTTTATAAACAGGGGTTCCCCCTTGAAGCTTTTCTATGTAGG
TCCTATGTTTCGCTACGAAAAACCTCAGGCAGGTAGATTGAGGCAGTTTCACCAATTTGGTATTGAAATACTGGGTTCGC
ATGATTCAACCGCAGATTACGAGGTTATAGAACTGGGGATAAACTTTCTGAAGGAATTGGGAATAAGTGGCACTAAATTG
TATATAAACAGTGTTGGTTGTAATAAATGTAGACCTAAGTATAACGAGGTACTAAAAGATTACTATTCGGACAAGCTCTC
CACGCTTTGTGATGATTGTAAGCGGCGATATAACACAAACGTTCTCAGACTTCTCGACTGCAAAATAGATAAAGAAGTGG
CAGAAGGTGCTCCATCGATACATAATTACCTTTGCGATGACTGCAAGATCCATTTCGAACAGCTGCAGTATTACCTTAAA
TCGAGAAACCTGGATTATGAAGTCGATCATAAGCTGGTAAGAGGGCTTGACTATTACACAAAAACCGCTTTCGAAATCAG
ATCAAACATGTTAGGAGCACAGGACCAGGTTCTTGCCGGGGGCCGATATGACGGACTTGTAGAATACATCGGAGGAAAAT
CCGTGCCTGCTGTTGGTTTTGCTGCCGGGATGGAGAGAATGATTCTTCTATTAAAAAAGATGGGAATTGAGCCGAAGGTT
GAAGAACTAAATACCGTTGCCATTCTTCCCATGAATAAAGAAGCCTTTGTAAAGGCTTTCGAATTTGCAGAAAGGCTCAG
AAAGAAAGGAATATCTACTTTTGTTGATGTTATGGAACGTAACTTGAGAAATAAATTCAAGCACGCGAGCAGAATTGGCG
CAAAAATTGCCGTTGTTATTGGTGAGGAAGAATTGGAAAAAGGTTTTGTTACCATAAAAGTCATGGAAGAAGGCAGTCAA
TATACGGTGGATTCGGACTGGATGGCCGACTACATCTTCAAGAAACTAAAAGAATTATAG

Upstream 100 bases:

>100_bases
GACTGGAGTTTCTTGTCGTAATATTCGGTATAACAAAGATGTTCAAAGATGCCTTTTATATAGCTACCGTTCGTAAAAGG
AAAGCTACGGAGGTGTAACC

Downstream 100 bases:

>100_bases
GCATTTTTGGTTGTTCAGGAGAGCATTTTAAAGATAAACAATGATAATCAGACTTAACTGTGTACAAAAACCTCTTGTGG
TGTGTTATCATACTTACGAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MITRIKGTQDVFFEEIKYWHYIENTIRKITKLYAFEEIRTPIFEHTELFKRSVGESTDVVQKEMYTFNDKGGRSLTLRPE
GTASVARAYVENGFINRGSPLKLFYVGPMFRYEKPQAGRLRQFHQFGIEILGSHDSTADYEVIELGINFLKELGISGTKL
YINSVGCNKCRPKYNEVLKDYYSDKLSTLCDDCKRRYNTNVLRLLDCKIDKEVAEGAPSIHNYLCDDCKIHFEQLQYYLK
SRNLDYEVDHKLVRGLDYYTKTAFEIRSNMLGAQDQVLAGGRYDGLVEYIGGKSVPAVGFAAGMERMILLLKKMGIEPKV
EELNTVAILPMNKEAFVKAFEFAERLRKKGISTFVDVMERNLRNKFKHASRIGAKIAVVIGEEELEKGFVTIKVMEEGSQ
YTVDSDWMADYIFKKLKEL

Sequences:

>Translated_419_residues
MITRIKGTQDVFFEEIKYWHYIENTIRKITKLYAFEEIRTPIFEHTELFKRSVGESTDVVQKEMYTFNDKGGRSLTLRPE
GTASVARAYVENGFINRGSPLKLFYVGPMFRYEKPQAGRLRQFHQFGIEILGSHDSTADYEVIELGINFLKELGISGTKL
YINSVGCNKCRPKYNEVLKDYYSDKLSTLCDDCKRRYNTNVLRLLDCKIDKEVAEGAPSIHNYLCDDCKIHFEQLQYYLK
SRNLDYEVDHKLVRGLDYYTKTAFEIRSNMLGAQDQVLAGGRYDGLVEYIGGKSVPAVGFAAGMERMILLLKKMGIEPKV
EELNTVAILPMNKEAFVKAFEFAERLRKKGISTFVDVMERNLRNKFKHASRIGAKIAVVIGEEELEKGFVTIKVMEEGSQ
YTVDSDWMADYIFKKLKEL
>Mature_419_residues
MITRIKGTQDVFFEEIKYWHYIENTIRKITKLYAFEEIRTPIFEHTELFKRSVGESTDVVQKEMYTFNDKGGRSLTLRPE
GTASVARAYVENGFINRGSPLKLFYVGPMFRYEKPQAGRLRQFHQFGIEILGSHDSTADYEVIELGINFLKELGISGTKL
YINSVGCNKCRPKYNEVLKDYYSDKLSTLCDDCKRRYNTNVLRLLDCKIDKEVAEGAPSIHNYLCDDCKIHFEQLQYYLK
SRNLDYEVDHKLVRGLDYYTKTAFEIRSNMLGAQDQVLAGGRYDGLVEYIGGKSVPAVGFAAGMERMILLLKKMGIEPKV
EELNTVAILPMNKEAFVKAFEFAERLRKKGISTFVDVMERNLRNKFKHASRIGAKIAVVIGEEELEKGFVTIKVMEEGSQ
YTVDSDWMADYIFKKLKEL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=441, Percent_Identity=25.1700680272109, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI15029520, Length=466, Percent_Identity=23.8197424892704, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1788861, Length=417, Percent_Identity=42.2062350119904, Blast_Score=331, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48303; Mature: 48303

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRIKGTQDVFFEEIKYWHYIENTIRKITKLYAFEEIRTPIFEHTELFKRSVGESTDVV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHH
QKEMYTFNDKGGRSLTLRPEGTASVARAYVENGFINRGSPLKLFYVGPMFRYEKPQAGRL
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEECCHHCCCCCCHHHH
RQFHQFGIEILGSHDSTADYEVIELGINFLKELGISGTKLYINSVGCNKCRPKYNEVLKD
HHHHHHCHHEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHH
YYSDKLSTLCDDCKRRYNTNVLRLLDCKIDKEVAEGAPSIHNYLCDDCKIHFEQLQYYLK
HHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SRNLDYEVDHKLVRGLDYYTKTAFEIRSNMLGAQDQVLAGGRYDGLVEYIGGKSVPAVGF
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHCCCCCCHHHH
AAGMERMILLLKKMGIEPKVEELNTVAILPMNKEAFVKAFEFAERLRKKGISTFVDVMER
HHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
NLRNKFKHASRIGAKIAVVIGEEELEKGFVTIKVMEEGSQYTVDSDWMADYIFKKLKEL
HHHHHHHHHHHCCCEEEEEECHHHHHCCEEEEEEEECCCCEEECHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITRIKGTQDVFFEEIKYWHYIENTIRKITKLYAFEEIRTPIFEHTELFKRSVGESTDVV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHH
QKEMYTFNDKGGRSLTLRPEGTASVARAYVENGFINRGSPLKLFYVGPMFRYEKPQAGRL
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEECCHHCCCCCCHHHH
RQFHQFGIEILGSHDSTADYEVIELGINFLKELGISGTKLYINSVGCNKCRPKYNEVLKD
HHHHHHCHHEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHH
YYSDKLSTLCDDCKRRYNTNVLRLLDCKIDKEVAEGAPSIHNYLCDDCKIHFEQLQYYLK
HHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SRNLDYEVDHKLVRGLDYYTKTAFEIRSNMLGAQDQVLAGGRYDGLVEYIGGKSVPAVGF
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHCCCCCCHHHH
AAGMERMILLLKKMGIEPKVEELNTVAILPMNKEAFVKAFEFAERLRKKGISTFVDVMER
HHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
NLRNKFKHASRIGAKIAVVIGEEELEKGFVTIKVMEEGSQYTVDSDWMADYIFKKLKEL
HHHHHHHHHHHCCCEEEEEECHHHHHCCEEEEEEEECCCCEEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]