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The map label for this gene is gdhA [H]

Identifier: 239616704

GI number: 239616704

Start: 322616

End: 323857

Strand: Reverse

Name: gdhA [H]

Synonym: Kole_0297

Alternate gene names: 239616704

Gene position: 323857-322616 (Counterclockwise)

Preceding gene: 239616705

Following gene: 239616703

Centisome position: 14.07

GC content: 42.59

Gene sequence:

>1242_bases
ATGGGGCTTTTTGAAATGGCTATCAGCAATTTTAACAGAGCAGTGGCCATAATGGGAATTGACGAAGAATATGCGGAAAT
GCTCAGGAGGCCTAAACGCTGTCTTATCGTTGATTTTCCTGTTTTGATGGATGACGGTAGTGTTAAAGTGTTCACAGGGT
ACAGAGTCCAACACAACACCGCACGGGGACCGGCCAAAGGGGGCATCAGGTATCATCCTGAAACAAACCTTGATGAGGTA
AAAGCTCTGGCATTCTGGATGACATGGAAAACCTCCCTGATGGATCTTCCGTTTGGAGGCGCAAAAGGAGGAGTCAGAGT
AGATCCGAAAAGTCTGAGTGAAAAAGAATTGCGTCGGCTTTCAAGACGATACTTTTCAGAAATTCAGATAATGATTGGTC
CCCAGCATGATATTCCTGCACCTGATGTCAATACGAATCCGGATATCATGGCGGTGTACATGGATACTTACAGTATGAAT
ATCGGACATACAGAACTGGGGGTTGTTACCGGCAAACCTGTGCGACTTGGTGGTTCAAAAGGTCGGGAAGAAGCAACCGG
TAGGGGTGTGATGGTGACGGTTAGGGAAGCTTGTAGAGAATTGGGAATTGAAACATCGAAGGCAACGGTTGCTGTTCAAG
GATTCGGAAATGTTGGAATGTACTCCGCTCTTCTGTGCAATCATGAACTTGGTTGCAAGATTATAGCTGTTAGCGATAGT
AAAGGTGGTATCTTTAACCCAAATGGGTTGAACATACAGGAACTCATTGAGCATAAAAAAAGTACTGGAAAGGTGGATAG
TTTTCCTGGAGGAGAGAGAATAGGAAAGGACGACGTTTTTGAAATGGATGTAGATATTTTGATACCAGCAGCGCTGGAAA
ATGCCATAACAGAGGATAATGCGCATAAAATAAAGGCGAAGATAATATCTGAAGGAGTAAATGGTCCTATTACCCCGGAA
GCGGATAAAATACTGAACCAACGAAGAGTTATGGTGATCCCGGATATTCTTGCTAATGCTGGTGGAGTGACTGTTTCTTA
TTTCGAATGGGTACAGGATCTTCAGGCTTTTTTCTGGAGTCTTGAACAAATAAGAGAGACTCTCGAATCGATGATGACAG
AAGCTTTTAAGGAAACACTTGATACGGCAAAAAAATATGGTGTTGATTTGAGAACAGCAGCATACATTATAGCTATTGAT
AGAGTCATGTATGCAATAAAAAAACGAGGAATTTATCCATAA

Upstream 100 bases:

>100_bases
ACTTGGTAAGTTCAATTGGTCAGAGGATCCTGAGTTGAAAAGAAAATACGGCGGCGATTGTGCCAACGATGAGTGAAATT
TCTTAATAGGGGGGATGAAC

Downstream 100 bases:

>100_bases
GAAAAAGATGAGAATTCATGTAATCATCTGTTTTTCTCGGCCTCCTTCAGGGTAAATAAGTGTAACTTTAAATTTATTAA
AAACACCCCTCGGAAACATT

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MGLFEMAISNFNRAVAIMGIDEEYAEMLRRPKRCLIVDFPVLMDDGSVKVFTGYRVQHNTARGPAKGGIRYHPETNLDEV
KALAFWMTWKTSLMDLPFGGAKGGVRVDPKSLSEKELRRLSRRYFSEIQIMIGPQHDIPAPDVNTNPDIMAVYMDTYSMN
IGHTELGVVTGKPVRLGGSKGREEATGRGVMVTVREACRELGIETSKATVAVQGFGNVGMYSALLCNHELGCKIIAVSDS
KGGIFNPNGLNIQELIEHKKSTGKVDSFPGGERIGKDDVFEMDVDILIPAALENAITEDNAHKIKAKIISEGVNGPITPE
ADKILNQRRVMVIPDILANAGGVTVSYFEWVQDLQAFFWSLEQIRETLESMMTEAFKETLDTAKKYGVDLRTAAYIIAID
RVMYAIKKRGIYP

Sequences:

>Translated_413_residues
MGLFEMAISNFNRAVAIMGIDEEYAEMLRRPKRCLIVDFPVLMDDGSVKVFTGYRVQHNTARGPAKGGIRYHPETNLDEV
KALAFWMTWKTSLMDLPFGGAKGGVRVDPKSLSEKELRRLSRRYFSEIQIMIGPQHDIPAPDVNTNPDIMAVYMDTYSMN
IGHTELGVVTGKPVRLGGSKGREEATGRGVMVTVREACRELGIETSKATVAVQGFGNVGMYSALLCNHELGCKIIAVSDS
KGGIFNPNGLNIQELIEHKKSTGKVDSFPGGERIGKDDVFEMDVDILIPAALENAITEDNAHKIKAKIISEGVNGPITPE
ADKILNQRRVMVIPDILANAGGVTVSYFEWVQDLQAFFWSLEQIRETLESMMTEAFKETLDTAKKYGVDLRTAAYIIAID
RVMYAIKKRGIYP
>Mature_412_residues
GLFEMAISNFNRAVAIMGIDEEYAEMLRRPKRCLIVDFPVLMDDGSVKVFTGYRVQHNTARGPAKGGIRYHPETNLDEVK
ALAFWMTWKTSLMDLPFGGAKGGVRVDPKSLSEKELRRLSRRYFSEIQIMIGPQHDIPAPDVNTNPDIMAVYMDTYSMNI
GHTELGVVTGKPVRLGGSKGREEATGRGVMVTVREACRELGIETSKATVAVQGFGNVGMYSALLCNHELGCKIIAVSDSK
GGIFNPNGLNIQELIEHKKSTGKVDSFPGGERIGKDDVFEMDVDILIPAALENAITEDNAHKIKAKIISEGVNGPITPEA
DKILNQRRVMVIPDILANAGGVTVSYFEWVQDLQAFFWSLEQIRETLESMMTEAFKETLDTAKKYGVDLRTAAYIIAIDR
VMYAIKKRGIYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=453, Percent_Identity=40.6181015452539, Blast_Score=311, Evalue=1e-84,
Organism=Homo sapiens, GI4885281, Length=453, Percent_Identity=40.3973509933775, Blast_Score=307, Evalue=1e-83,
Organism=Escherichia coli, GI1788059, Length=362, Percent_Identity=33.9779005524862, Blast_Score=177, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17544676, Length=443, Percent_Identity=35.2144469525959, Blast_Score=263, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324951, Length=394, Percent_Identity=34.5177664974619, Blast_Score=166, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6319256, Length=396, Percent_Identity=32.3232323232323, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI45553475, Length=451, Percent_Identity=36.8070953436807, Blast_Score=274, Evalue=9e-74,
Organism=Drosophila melanogaster, GI45549226, Length=464, Percent_Identity=35.3448275862069, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24649283, Length=346, Percent_Identity=41.0404624277457, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24649460, Length=403, Percent_Identity=33.9950372208437, Blast_Score=217, Evalue=2e-56,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 45730; Mature: 45599

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLFEMAISNFNRAVAIMGIDEEYAEMLRRPKRCLIVDFPVLMDDGSVKVFTGYRVQHNT
CCHHHHHHHCCCCEEEEEECCHHHHHHHHCCCEEEEEEECEEECCCCEEEEEEEEEECCC
ARGPAKGGIRYHPETNLDEVKALAFWMTWKTSLMDLPFGGAKGGVRVDPKSLSEKELRRL
CCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHCCCHHHHHHH
SRRYFSEIQIMIGPQHDIPAPDVNTNPDIMAVYMDTYSMNIGHTELGVVTGKPVRLGGSK
HHHHHHHEEEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCEEEEEECCCEEECCCC
GREEATGRGVMVTVREACRELGIETSKATVAVQGFGNVGMYSALLCNHELGCKIIAVSDS
CCCHHCCCCEEEEHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEEECC
KGGIFNPNGLNIQELIEHKKSTGKVDSFPGGERIGKDDVFEMDVDILIPAALENAITEDN
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECHHEEEHHHHHHHHCCCC
AHKIKAKIISEGVNGPITPEADKILNQRRVMVIPDILANAGGVTVSYFEWVQDLQAFFWS
HHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEECHHHCCCCCCCHHHHHHHHHHHHHHHH
LEQIRETLESMMTEAFKETLDTAKKYGVDLRTAAYIIAIDRVMYAIKKRGIYP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GLFEMAISNFNRAVAIMGIDEEYAEMLRRPKRCLIVDFPVLMDDGSVKVFTGYRVQHNT
CHHHHHHHCCCCEEEEEECCHHHHHHHHCCCEEEEEEECEEECCCCEEEEEEEEEECCC
ARGPAKGGIRYHPETNLDEVKALAFWMTWKTSLMDLPFGGAKGGVRVDPKSLSEKELRRL
CCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHCCCHHHHHHH
SRRYFSEIQIMIGPQHDIPAPDVNTNPDIMAVYMDTYSMNIGHTELGVVTGKPVRLGGSK
HHHHHHHEEEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCEEEEEECCCEEECCCC
GREEATGRGVMVTVREACRELGIETSKATVAVQGFGNVGMYSALLCNHELGCKIIAVSDS
CCCHHCCCCEEEEHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEEECC
KGGIFNPNGLNIQELIEHKKSTGKVDSFPGGERIGKDDVFEMDVDILIPAALENAITEDN
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECHHEEEHHHHHHHHCCCC
AHKIKAKIISEGVNGPITPEADKILNQRRVMVIPDILANAGGVTVSYFEWVQDLQAFFWS
HHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEECHHHCCCCCCCHHHHHHHHHHHHHHHH
LEQIRETLESMMTEAFKETLDTAKKYGVDLRTAAYIIAIDRVMYAIKKRGIYP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]