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The map label for this gene is leuS [H]

Identifier: 239616581

GI number: 239616581

Start: 194262

End: 196637

Strand: Reverse

Name: leuS [H]

Synonym: Kole_0173

Alternate gene names: 239616581

Gene position: 196637-194262 (Counterclockwise)

Preceding gene: 239616582

Following gene: 239616580

Centisome position: 8.54

GC content: 44.7

Gene sequence:

>2376_bases
ATGTACGATTTCAAGTCGATAGAAAAGAAGTGGCAAAAGTATTATGAGGAAGAAGAGCCTTTCAAGGTTGATTTGAAAAA
CGCGAAAAAACCCTTTTACAACCTCATGATGTTTCCGTATCCTTCGGCTTCCGGGTTACATATAGGGAATATGTATTCTT
TTATAGGTTCAGACGTTTACGGGCGTTATAAAAAGCTTCAGGGCTACGATGTTTTTGAACCAATAGGATTTGATGCTTTT
GGTATCCACAGTGAAAATTACGCTCTAAAAGTTGGCGAGCACCCTGCAAAGCTCACACCCAAAAGTATCAAATACTTCAG
GGATGAGCAGCTGAAAAAGATCGGGAATATCTATGACTGGAGCAGTGAAGTCAACACCTCTTCACCCGAATATTACAAGT
GGACCCAGTGGATATTCCTTCAACTCTACAAAGCCGGGCTCGCAGAGAAGAAAAACTCCGAGGTCAACTGGTGCCCTTCG
TGTAAGACGGTTCTCGCCGACGAACAGGTTATAGACGGAAAATGTGAACGTTGTAGCACTGAAATAACGAAGCGGGAGAT
GAGCCAGTGGTTCTTCAAGATCACAAAATACGCTGACCGCCTCCTTGAAAACCTGGAAAAACTCGATTGGACCGAAACCA
CAAAGAATATCCAGCGTAGCTGGATTGGGAGATCTGAAGGGGCAAAGATCCGTTTCCCGGTTGAGAATTCCGATATTGAG
ATAGAGGTATTCACCACGAGGCCAGACACGATCTATGGTGTTACTTACCTTACTTTAGCTCCGGAACACAAGTTGGTTAA
AAAACTCATAAAAGAAGATAAATTTTCGCAGTTTGAAGAATTCCTTGAGAGCGTTAAGAAGATGGACGTCGCTACCAGAA
CGTCCAAGACGCGCCCTAAAACTGGTATGTTCACGGGAAGTTATGCTATTCACCCGCTCACTGGTGAAAGAATTCCTATC
TGGGTTGGTGACTACGTGCTTGTGGAATACGGTACCGGTGCGGTAATGGCTGTTCCCGCACATGATGAGAGGGACTACGC
TTTTGCGAAAAAATACGGGCTCGAAATCAAGCAGGTTATCGAGTGTGATCCTGAGGATTTGCCCTACACTGAGAAGGGGA
TAATGATAAACAGCGGTCCATATACAGGCTGGAAGAGCGAGGATTTTATCGCCAATATAGACAGTGTGCACGAGAAGATA
GAATCGGCCGTCACCTATCACCTTCACGATTGGTGTGTCTCAAGGCAGAGATACTGGGGACCACCAATCCCAATAATATA
CTGTGATAAATGTGGTATCGTTCCTGTTCCTGAAGAGGATCTGCCTGTTTTGCTTCCCGAAACGGACGACTATATCCCGG
ATGGCAGCGGAAAGTCCCCTCTGGCGAGGAATGAGGAGTTCGTCAATACCACCTGTCCTAAATGTGGTGGTCCGGCTAAA
AGAGAGACAGATGTCAGCGATAATTTCCTTGACTCGGCATGGTATTTCTTGAGGTATATATCACCACACGATCAGGATAG
ACCTTTCGATCCTGAGCTGGCTAAAAAATGGCTTCCGGTGGATATGTACATCGGAGGTAACGAACATGCAAACCTACATC
TAATGTACGCGCGATTCATTACCATGGCACTTCATGATCTCGGAATTCTCCACTTTGAAGAGCCATTCAAATCCTTCAGG
GGCCACGGTCTGATAATCAAAGATGGGGAGAAAATGAGCAAATCAAAGGGAAACATCGTCAACCCAAACGACTATTTCGA
TACCCACGGTGTTGATGCGCTAAGAACTTACCTTATGTTTATGGGAAGCTTCCTTGAAGGCGGTGACTTCAGGGACAGCG
GAATGGATGCCATCAAACGTTTCCTCAACAGGGTATGGGAGGTTGCCAACAAACCCGAGGGTGAAAGTTCTCTCCAGCTA
AAGATCAAGATGGCAGAGACCATTAAAAAGGTGGAGTATTCGATTGAAAATATCAAGTACAATACGGCTATCGCTGCCAT
TATGGAATTTATAAATGAGGCTGTTAAAGAAGAACGCATAGAAAGGCAGCTGGTTGTGGATCTGGCAAAACTCCTTGCTC
CTTTTGCACCCTTCATTTGTGAGGAAATACACGAAATGAAGGGCGGCAAAGGAACTATATTGAATGCCGGATGGCCCACA
GGATATGACAAGTACCTTTCTATGGCGGATGTGGAGATTCCGGTGCAGATAAACGGTAAGATGCGTGGAAAAGTAAAAAT
TCATCAGAAAGCTTCGGAAGATGAGGTTATAGAGAAGATAAAAGGCGATGAAAGGCTTGCGCACTACCTCAATAAAGGAG
AGATACGAAAGGTTATATACGTCCAGGACAAAATAATTAACATTATTGTGAAGTAA

Upstream 100 bases:

>100_bases
CGTATCTGTGGTGGAAAAAAGTGCGAGCTGTGCTATAATTTTGAAAACTGCTCTCGGTCGGAAATCTATTATTTGACCAG
CTTAGGGAGGGGTACGAAGT

Downstream 100 bases:

>100_bases
TAACAGGAAGTTGTGGGGGAGGTTCTACCGAACCTCCCTTCTTAGTTTGGAGAAAATTTTAACTTTATACATTGTAACTT
CATAGAATGATATGGTAGAA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 791; Mature: 791

Protein sequence:

>791_residues
MYDFKSIEKKWQKYYEEEEPFKVDLKNAKKPFYNLMMFPYPSASGLHIGNMYSFIGSDVYGRYKKLQGYDVFEPIGFDAF
GIHSENYALKVGEHPAKLTPKSIKYFRDEQLKKIGNIYDWSSEVNTSSPEYYKWTQWIFLQLYKAGLAEKKNSEVNWCPS
CKTVLADEQVIDGKCERCSTEITKREMSQWFFKITKYADRLLENLEKLDWTETTKNIQRSWIGRSEGAKIRFPVENSDIE
IEVFTTRPDTIYGVTYLTLAPEHKLVKKLIKEDKFSQFEEFLESVKKMDVATRTSKTRPKTGMFTGSYAIHPLTGERIPI
WVGDYVLVEYGTGAVMAVPAHDERDYAFAKKYGLEIKQVIECDPEDLPYTEKGIMINSGPYTGWKSEDFIANIDSVHEKI
ESAVTYHLHDWCVSRQRYWGPPIPIIYCDKCGIVPVPEEDLPVLLPETDDYIPDGSGKSPLARNEEFVNTTCPKCGGPAK
RETDVSDNFLDSAWYFLRYISPHDQDRPFDPELAKKWLPVDMYIGGNEHANLHLMYARFITMALHDLGILHFEEPFKSFR
GHGLIIKDGEKMSKSKGNIVNPNDYFDTHGVDALRTYLMFMGSFLEGGDFRDSGMDAIKRFLNRVWEVANKPEGESSLQL
KIKMAETIKKVEYSIENIKYNTAIAAIMEFINEAVKEERIERQLVVDLAKLLAPFAPFICEEIHEMKGGKGTILNAGWPT
GYDKYLSMADVEIPVQINGKMRGKVKIHQKASEDEVIEKIKGDERLAHYLNKGEIRKVIYVQDKIINIIVK

Sequences:

>Translated_791_residues
MYDFKSIEKKWQKYYEEEEPFKVDLKNAKKPFYNLMMFPYPSASGLHIGNMYSFIGSDVYGRYKKLQGYDVFEPIGFDAF
GIHSENYALKVGEHPAKLTPKSIKYFRDEQLKKIGNIYDWSSEVNTSSPEYYKWTQWIFLQLYKAGLAEKKNSEVNWCPS
CKTVLADEQVIDGKCERCSTEITKREMSQWFFKITKYADRLLENLEKLDWTETTKNIQRSWIGRSEGAKIRFPVENSDIE
IEVFTTRPDTIYGVTYLTLAPEHKLVKKLIKEDKFSQFEEFLESVKKMDVATRTSKTRPKTGMFTGSYAIHPLTGERIPI
WVGDYVLVEYGTGAVMAVPAHDERDYAFAKKYGLEIKQVIECDPEDLPYTEKGIMINSGPYTGWKSEDFIANIDSVHEKI
ESAVTYHLHDWCVSRQRYWGPPIPIIYCDKCGIVPVPEEDLPVLLPETDDYIPDGSGKSPLARNEEFVNTTCPKCGGPAK
RETDVSDNFLDSAWYFLRYISPHDQDRPFDPELAKKWLPVDMYIGGNEHANLHLMYARFITMALHDLGILHFEEPFKSFR
GHGLIIKDGEKMSKSKGNIVNPNDYFDTHGVDALRTYLMFMGSFLEGGDFRDSGMDAIKRFLNRVWEVANKPEGESSLQL
KIKMAETIKKVEYSIENIKYNTAIAAIMEFINEAVKEERIERQLVVDLAKLLAPFAPFICEEIHEMKGGKGTILNAGWPT
GYDKYLSMADVEIPVQINGKMRGKVKIHQKASEDEVIEKIKGDERLAHYLNKGEIRKVIYVQDKIINIIVK
>Mature_791_residues
MYDFKSIEKKWQKYYEEEEPFKVDLKNAKKPFYNLMMFPYPSASGLHIGNMYSFIGSDVYGRYKKLQGYDVFEPIGFDAF
GIHSENYALKVGEHPAKLTPKSIKYFRDEQLKKIGNIYDWSSEVNTSSPEYYKWTQWIFLQLYKAGLAEKKNSEVNWCPS
CKTVLADEQVIDGKCERCSTEITKREMSQWFFKITKYADRLLENLEKLDWTETTKNIQRSWIGRSEGAKIRFPVENSDIE
IEVFTTRPDTIYGVTYLTLAPEHKLVKKLIKEDKFSQFEEFLESVKKMDVATRTSKTRPKTGMFTGSYAIHPLTGERIPI
WVGDYVLVEYGTGAVMAVPAHDERDYAFAKKYGLEIKQVIECDPEDLPYTEKGIMINSGPYTGWKSEDFIANIDSVHEKI
ESAVTYHLHDWCVSRQRYWGPPIPIIYCDKCGIVPVPEEDLPVLLPETDDYIPDGSGKSPLARNEEFVNTTCPKCGGPAK
RETDVSDNFLDSAWYFLRYISPHDQDRPFDPELAKKWLPVDMYIGGNEHANLHLMYARFITMALHDLGILHFEEPFKSFR
GHGLIIKDGEKMSKSKGNIVNPNDYFDTHGVDALRTYLMFMGSFLEGGDFRDSGMDAIKRFLNRVWEVANKPEGESSLQL
KIKMAETIKKVEYSIENIKYNTAIAAIMEFINEAVKEERIERQLVVDLAKLLAPFAPFICEEIHEMKGGKGTILNAGWPT
GYDKYLSMADVEIPVQINGKMRGKVKIHQKASEDEVIEKIKGDERLAHYLNKGEIRKVIYVQDKIINIIVK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=873, Percent_Identity=31.1569301260023, Blast_Score=422, Evalue=1e-118,
Organism=Escherichia coli, GI1786861, Length=867, Percent_Identity=39.3310265282584, Blast_Score=613, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI71997510, Length=822, Percent_Identity=28.8321167883212, Blast_Score=285, Evalue=7e-77,
Organism=Caenorhabditis elegans, GI71997517, Length=820, Percent_Identity=28.780487804878, Blast_Score=284, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI212645227, Length=336, Percent_Identity=25.5952380952381, Blast_Score=100, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323414, Length=877, Percent_Identity=33.6374002280502, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI21355409, Length=887, Percent_Identity=29.6505073280722, Blast_Score=350, Evalue=3e-96,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 91149; Mature: 91149

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDFKSIEKKWQKYYEEEEPFKVDLKNAKKPFYNLMMFPYPSASGLHIGNMYSFIGSDVY
CCCHHHHHHHHHHHCCCCCCEEEEHHHCCCCHHEEEEECCCCCCCCCHHHHHHHHCCHHH
GRYKKLQGYDVFEPIGFDAFGIHSENYALKVGEHPAKLTPKSIKYFRDEQLKKIGNIYDW
HHHHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC
SSEVNTSSPEYYKWTQWIFLQLYKAGLAEKKNSEVNWCPSCKTVLADEQVIDGKCERCST
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHCCHHHHHHH
EITKREMSQWFFKITKYADRLLENLEKLDWTETTKNIQRSWIGRSEGAKIRFPVENSDIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEE
IEVFTTRPDTIYGVTYLTLAPEHKLVKKLIKEDKFSQFEEFLESVKKMDVATRTSKTRPK
EEEEECCCCEEEEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCC
TGMFTGSYAIHPLTGERIPIWVGDYVLVEYGTGAVMAVPAHDERDYAFAKKYGLEIKQVI
CCEEECCEEEECCCCCEEEEEECCEEEEEECCCEEEEECCCCCCCHHHHHHHCCCHHHHH
ECDPEDLPYTEKGIMINSGPYTGWKSEDFIANIDSVHEKIESAVTYHLHDWCVSRQRYWG
CCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
PPIPIIYCDKCGIVPVPEEDLPVLLPETDDYIPDGSGKSPLARNEEFVNTTCPKCGGPAK
CCCCEEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCC
RETDVSDNFLDSAWYFLRYISPHDQDRPFDPELAKKWLPVDMYIGGNEHANLHLMYARFI
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCEEEHHHHHH
TMALHDLGILHFEEPFKSFRGHGLIIKDGEKMSKSKGNIVNPNDYFDTHGVDALRTYLMF
HHHHHHHCHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
MGSFLEGGDFRDSGMDAIKRFLNRVWEVANKPEGESSLQLKIKMAETIKKVEYSIENIKY
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHEEH
NTAIAAIMEFINEAVKEERIERQLVVDLAKLLAPFAPFICEEIHEMKGGKGTILNAGWPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
GYDKYLSMADVEIPVQINGKMRGKVKIHQKASEDEVIEKIKGDERLAHYLNKGEIRKVIY
CHHHHHHHCCEEEEEEECCCEECEEEEECCCCHHHHHHHHCCHHHHHHHHCCCCEEEEEE
VQDKIINIIVK
EECCCEEEECC
>Mature Secondary Structure
MYDFKSIEKKWQKYYEEEEPFKVDLKNAKKPFYNLMMFPYPSASGLHIGNMYSFIGSDVY
CCCHHHHHHHHHHHCCCCCCEEEEHHHCCCCHHEEEEECCCCCCCCCHHHHHHHHCCHHH
GRYKKLQGYDVFEPIGFDAFGIHSENYALKVGEHPAKLTPKSIKYFRDEQLKKIGNIYDW
HHHHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC
SSEVNTSSPEYYKWTQWIFLQLYKAGLAEKKNSEVNWCPSCKTVLADEQVIDGKCERCST
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHCCHHHHHHH
EITKREMSQWFFKITKYADRLLENLEKLDWTETTKNIQRSWIGRSEGAKIRFPVENSDIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEE
IEVFTTRPDTIYGVTYLTLAPEHKLVKKLIKEDKFSQFEEFLESVKKMDVATRTSKTRPK
EEEEECCCCEEEEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCC
TGMFTGSYAIHPLTGERIPIWVGDYVLVEYGTGAVMAVPAHDERDYAFAKKYGLEIKQVI
CCEEECCEEEECCCCCEEEEEECCEEEEEECCCEEEEECCCCCCCHHHHHHHCCCHHHHH
ECDPEDLPYTEKGIMINSGPYTGWKSEDFIANIDSVHEKIESAVTYHLHDWCVSRQRYWG
CCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
PPIPIIYCDKCGIVPVPEEDLPVLLPETDDYIPDGSGKSPLARNEEFVNTTCPKCGGPAK
CCCCEEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCC
RETDVSDNFLDSAWYFLRYISPHDQDRPFDPELAKKWLPVDMYIGGNEHANLHLMYARFI
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCEEEHHHHHH
TMALHDLGILHFEEPFKSFRGHGLIIKDGEKMSKSKGNIVNPNDYFDTHGVDALRTYLMF
HHHHHHHCHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
MGSFLEGGDFRDSGMDAIKRFLNRVWEVANKPEGESSLQLKIKMAETIKKVEYSIENIKY
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHEEH
NTAIAAIMEFINEAVKEERIERQLVVDLAKLLAPFAPFICEEIHEMKGGKGTILNAGWPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
GYDKYLSMADVEIPVQINGKMRGKVKIHQKASEDEVIEKIKGDERLAHYLNKGEIRKVIY
CHHHHHHHCCEEEEEEECCCEECEEEEECCCCHHHHHHHHCCHHHHHHHHCCCCEEEEEE
VQDKIINIIVK
EECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]