Click here to switch to the map view.

The map label for this gene is celM [H]

Identifier: 239616449

GI number: 239616449

Start: 44624

End: 45634

Strand: Reverse

Name: celM [H]

Synonym: Kole_0038

Alternate gene names: 239616449

Gene position: 45634-44624 (Counterclockwise)

Preceding gene: 239616450

Following gene: 239616448

Centisome position: 1.98

GC content: 45.6

Gene sequence:

>1011_bases
ATGAAAGAATTGATAAAGAAAGTGACAGAGGTTTGTTCTCCAAGCGGCCGCGAGGATAAAATAAGGGAAATGATTATTGA
GGAGATAAAGGATTTTGTTGATGGTTATAAAGTGGATCGTCTTGGAAACCTCGTGGCATGGAAAAAAGGTTCATCCGGCA
AAAAGCTTTTGCTTGATGCTCATATGGACGAGATCGGTGTTGTGGTAAGCAATATAGACGACAAGGGATTCCTCAAGATC
GAGATGATTGGTGGTGTTTCTCCGTACATTCTTCTCGGTACAAGAATAAGGTTTGAAACAGGTGTTATCGGGGTTGTTGG
TGTTGAAGGTGAGTCTGGTAAAGAATTACAGGAGAATATGAAGGGTCTTAGTTTTGACAACATTTTCGTGGATATCGGTG
CCACAAGCAGAGAAGAAGCGGGGAAGCTTGCACCGGTGGGAACCTTTGGAACTTACGACGCAACCTTCTACGATGCCGGG
AAGAGGTTGATATCCAAAGCAATGGATGACAGGATCGCCTGTGCAATCCTGATACAGGTTGCGAAGGAACTGAAGAATCC
GAAGGACGATATCTACCTTGCTTTTACCGTCCAGGAAGAGGTAGGACTTGTTGGGGCAAGCGTAGCCGGATTTGACATCA
TGCCGGATATGGCGATAGCTGTCGATATAACCGGCGGACCCGATACCCCGAAATCCTACAAAAGAATGGGCTTTGAACTC
GGTAAGGGTCCGGCGATAAAGATAAAGGACAGGGCAAGCGTGAGCTCTTCCTGGGTTGTGAAAAAGCTGGCAGAGGTTGC
AGAAAAGTATAAGATTCCCTATCAGTACGAGGTTTTGATATTTGGTGGCACAAATGCCAGAGGCTATCAGGTTACCAGAA
GCGGTATCGCTGCTGGAACTTTGTCAATTCCCACAAGATATGCCCACACACCCCATGAAATGGTTGATTATGACGATGTT
CTTAATGCGGTAAAACTCCTGAAAGCACTTTGTGAGGAAGGTATTGGATGA

Upstream 100 bases:

>100_bases
GTTACATACATTCGCCTGTTTCAGTGATAGATATCAATGATTTCTTGAAAGTTGTAAAACTTGCGAAAATCCTGGTTAGG
GAAGGGAAGGTGTTAAGCGA

Downstream 100 bases:

>100_bases
AAAAGTTCCTTTTCACGAGGGTTCAGGAATCTGAGTGTGTGGTGAAATTTGTCGAAGATTGCAGGAAAAACATACCGGAG
TCCCTTTTGATAGTGGACTC

Product: peptidase M42 family protein

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKELIKKVTEVCSPSGREDKIREMIIEEIKDFVDGYKVDRLGNLVAWKKGSSGKKLLLDAHMDEIGVVVSNIDDKGFLKI
EMIGGVSPYILLGTRIRFETGVIGVVGVEGESGKELQENMKGLSFDNIFVDIGATSREEAGKLAPVGTFGTYDATFYDAG
KRLISKAMDDRIACAILIQVAKELKNPKDDIYLAFTVQEEVGLVGASVAGFDIMPDMAIAVDITGGPDTPKSYKRMGFEL
GKGPAIKIKDRASVSSSWVVKKLAEVAEKYKIPYQYEVLIFGGTNARGYQVTRSGIAAGTLSIPTRYAHTPHEMVDYDDV
LNAVKLLKALCEEGIG

Sequences:

>Translated_336_residues
MKELIKKVTEVCSPSGREDKIREMIIEEIKDFVDGYKVDRLGNLVAWKKGSSGKKLLLDAHMDEIGVVVSNIDDKGFLKI
EMIGGVSPYILLGTRIRFETGVIGVVGVEGESGKELQENMKGLSFDNIFVDIGATSREEAGKLAPVGTFGTYDATFYDAG
KRLISKAMDDRIACAILIQVAKELKNPKDDIYLAFTVQEEVGLVGASVAGFDIMPDMAIAVDITGGPDTPKSYKRMGFEL
GKGPAIKIKDRASVSSSWVVKKLAEVAEKYKIPYQYEVLIFGGTNARGYQVTRSGIAAGTLSIPTRYAHTPHEMVDYDDV
LNAVKLLKALCEEGIG
>Mature_336_residues
MKELIKKVTEVCSPSGREDKIREMIIEEIKDFVDGYKVDRLGNLVAWKKGSSGKKLLLDAHMDEIGVVVSNIDDKGFLKI
EMIGGVSPYILLGTRIRFETGVIGVVGVEGESGKELQENMKGLSFDNIFVDIGATSREEAGKLAPVGTFGTYDATFYDAG
KRLISKAMDDRIACAILIQVAKELKNPKDDIYLAFTVQEEVGLVGASVAGFDIMPDMAIAVDITGGPDTPKSYKRMGFEL
GKGPAIKIKDRASVSSSWVVKKLAEVAEKYKIPYQYEVLIFGGTNARGYQVTRSGIAAGTLSIPTRYAHTPHEMVDYDDV
LNAVKLLKALCEEGIG

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=347, Percent_Identity=32.8530259365994, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI87082417, Length=335, Percent_Identity=32.2388059701493, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788727, Length=338, Percent_Identity=26.9230769230769, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 36573; Mature: 36573

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELIKKVTEVCSPSGREDKIREMIIEEIKDFVDGYKVDRLGNLVAWKKGSSGKKLLLDA
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCEEEECCCCCCCEEEEEC
HMDEIGVVVSNIDDKGFLKIEMIGGVSPYILLGTRIRFETGVIGVVGVEGESGKELQENM
CCCHHCEEEECCCCCCEEEEEEECCCCCEEEEEEEEEEECCEEEEEEECCCCCHHHHHHH
KGLSFDNIFVDIGATSREEAGKLAPVGTFGTYDATFYDAGKRLISKAMDDRIACAILIQV
CCCCCCCEEEECCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKELKNPKDDIYLAFTVQEEVGLVGASVAGFDIMPDMAIAVDITGGPDTPKSYKRMGFEL
HHHHCCCCCCEEEEEEEEHHCCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCC
GKGPAIKIKDRASVSSSWVVKKLAEVAEKYKIPYQYEVLIFGGTNARGYQVTRSGIAAGT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEECCCCCCCE
LSIPTRYAHTPHEMVDYDDVLNAVKLLKALCEEGIG
EECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKELIKKVTEVCSPSGREDKIREMIIEEIKDFVDGYKVDRLGNLVAWKKGSSGKKLLLDA
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCEEEECCCCCCCEEEEEC
HMDEIGVVVSNIDDKGFLKIEMIGGVSPYILLGTRIRFETGVIGVVGVEGESGKELQENM
CCCHHCEEEECCCCCCEEEEEEECCCCCEEEEEEEEEEECCEEEEEEECCCCCHHHHHHH
KGLSFDNIFVDIGATSREEAGKLAPVGTFGTYDATFYDAGKRLISKAMDDRIACAILIQV
CCCCCCCEEEECCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKELKNPKDDIYLAFTVQEEVGLVGASVAGFDIMPDMAIAVDITGGPDTPKSYKRMGFEL
HHHHCCCCCCEEEEEEEEHHCCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCC
GKGPAIKIKDRASVSSSWVVKKLAEVAEKYKIPYQYEVLIFGGTNARGYQVTRSGIAAGT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEECCCCCCCE
LSIPTRYAHTPHEMVDYDDVLNAVKLLKALCEEGIG
EECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA