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The map label for this gene is trmB [H]

Identifier: 239616414

GI number: 239616414

Start: 2431

End: 3375

Strand: Reverse

Name: trmB [H]

Synonym: Kole_0003

Alternate gene names: 239616414

Gene position: 3375-2431 (Counterclockwise)

Preceding gene: 239616433

Following gene: 239616413

Centisome position: 0.15

GC content: 41.16

Gene sequence:

>945_bases
ATGTGTCAGGATTTTACAAAGTTCTATGTTGAACCGCGTTTGATGAATCTTCCAATGGATCTTGATGCTCTTTTTACCAG
ATCTGCACCATTATTTCTAGAGGTGGGTTTTGGAAATGGAGAATTTCTGATAGAAAACGCGCTGAATAGACAGGGTCACA
ACTTCCTGGGTATAGAACTTTCTGTTATTTCAATGGTCAAAATAATGAAACGATTGTCAAGGGAAAACATCGAGAATGTG
AAAGTGATGATGGTTGACGCCAAATTCGGAGTCAGGGAACTCTTTCCTGATGAAAGTGTCGATGGTTTATATATGAATTT
CCCGTGCCCCTGGCCGCGTGAAAGGCATTCTGGTAAACGCCTGAACACGGTTGATTTTCTTCTCAGTGTGGCAGCGGTTC
TTAAAAAAGGTGGTATGGTTCAGCTGTACACTGATTCCCTGGAATTTGCGGAGGTTTTTGCTGAAAATCTCGGTCAAACC
TCATGTTTCATAAAGATAGAATTGATAAAGAATCCTCCGCAGGTAAATACCCGTTATGAACGAAAGTGGAAGCGTGAAGG
ACGAGAGATCTACAAGGTCACCGCTGAAAAGTTGAAAACACCCATGTTGAGACGAATAGCGGGAGGTGATGAAATGCCCC
ATACATGGATAAAGGAACTCAAACCAGAAAATTTAAAGAAAATTCTAAATTCTCCGATTAAGAAAGATATAGGTGTCTTC
GTATATAAGAGTATGCTAAAAGATCTGGATGACGATCGTTATCTTGTAGAAACGATAGCAATCGATGGTGATTTTCAGCA
GCATTTCTATGTAGAAATCCACAAACGAAACAAGGGCTGGTTGGTCAAATTGGACAGTCACGGACTTCCCTTCCGAACTC
CGGCAGTGAAAAAGGCTGTTTATGAACTTGGAAAGATTCTTGAAAAGAGGAGGGGAGCACTATGA

Upstream 100 bases:

>100_bases
AGTGGTTAATTAAGCGAACAGCAACCGCGATTTTGTGATTTATGATATATTTGTTCTGCAATAGGCAGATAAACAAAGAG
TTTTTCGGAGGTAATTTTAT

Downstream 100 bases:

>100_bases
GAATAAGTGTAATAGGTGCTGGAAGCTGGGGAAGCACTATGGCAAAAGTACTTGCGGATAGCGGACATGACGTTATGCTA
TGGGTCAGGGAACCGGATCT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MCQDFTKFYVEPRLMNLPMDLDALFTRSAPLFLEVGFGNGEFLIENALNRQGHNFLGIELSVISMVKIMKRLSRENIENV
KVMMVDAKFGVRELFPDESVDGLYMNFPCPWPRERHSGKRLNTVDFLLSVAAVLKKGGMVQLYTDSLEFAEVFAENLGQT
SCFIKIELIKNPPQVNTRYERKWKREGREIYKVTAEKLKTPMLRRIAGGDEMPHTWIKELKPENLKKILNSPIKKDIGVF
VYKSMLKDLDDDRYLVETIAIDGDFQQHFYVEIHKRNKGWLVKLDSHGLPFRTPAVKKAVYELGKILEKRRGAL

Sequences:

>Translated_314_residues
MCQDFTKFYVEPRLMNLPMDLDALFTRSAPLFLEVGFGNGEFLIENALNRQGHNFLGIELSVISMVKIMKRLSRENIENV
KVMMVDAKFGVRELFPDESVDGLYMNFPCPWPRERHSGKRLNTVDFLLSVAAVLKKGGMVQLYTDSLEFAEVFAENLGQT
SCFIKIELIKNPPQVNTRYERKWKREGREIYKVTAEKLKTPMLRRIAGGDEMPHTWIKELKPENLKKILNSPIKKDIGVF
VYKSMLKDLDDDRYLVETIAIDGDFQQHFYVEIHKRNKGWLVKLDSHGLPFRTPAVKKAVYELGKILEKRRGAL
>Mature_314_residues
MCQDFTKFYVEPRLMNLPMDLDALFTRSAPLFLEVGFGNGEFLIENALNRQGHNFLGIELSVISMVKIMKRLSRENIENV
KVMMVDAKFGVRELFPDESVDGLYMNFPCPWPRERHSGKRLNTVDFLLSVAAVLKKGGMVQLYTDSLEFAEVFAENLGQT
SCFIKIELIKNPPQVNTRYERKWKREGREIYKVTAEKLKTPMLRRIAGGDEMPHTWIKELKPENLKKILNSPIKKDIGVF
VYKSMLKDLDDDRYLVETIAIDGDFQQHFYVEIHKRNKGWLVKLDSHGLPFRTPAVKKAVYELGKILEKRRGAL

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=186, Percent_Identity=29.5698924731183, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 36377; Mature: 36377

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQDFTKFYVEPRLMNLPMDLDALFTRSAPLFLEVGFGNGEFLIENALNRQGHNFLGIEL
CCCHHHHHHCCCHHCCCCCCHHHHHHCCCCEEEEEECCCCCCCEEHHHCCCCCEEEEEEH
SVISMVKIMKRLSRENIENVKVMMVDAKFGVRELFPDESVDGLYMNFPCPWPRERHSGKR
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCCCCCEEEECCCCCCCHHCCCCC
LNTVDFLLSVAAVLKKGGMVQLYTDSLEFAEVFAENLGQTSCFIKIELIKNPPQVNTRYE
CHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHH
RKWKREGREIYKVTAEKLKTPMLRRIAGGDEMPHTWIKELKPENLKKILNSPIKKDIGVF
HHHHHCCHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHCCHHHHHHHHCCHHHHHHHHH
VYKSMLKDLDDDRYLVETIAIDGDFQQHFYVEIHKRNKGWLVKLDSHGLPFRTPAVKKAV
HHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCEEEEECCCCCCCCCHHHHHHH
YELGKILEKRRGAL
HHHHHHHHHHCCCC
>Mature Secondary Structure
MCQDFTKFYVEPRLMNLPMDLDALFTRSAPLFLEVGFGNGEFLIENALNRQGHNFLGIEL
CCCHHHHHHCCCHHCCCCCCHHHHHHCCCCEEEEEECCCCCCCEEHHHCCCCCEEEEEEH
SVISMVKIMKRLSRENIENVKVMMVDAKFGVRELFPDESVDGLYMNFPCPWPRERHSGKR
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCCCCCEEEECCCCCCCHHCCCCC
LNTVDFLLSVAAVLKKGGMVQLYTDSLEFAEVFAENLGQTSCFIKIELIKNPPQVNTRYE
CHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHH
RKWKREGREIYKVTAEKLKTPMLRRIAGGDEMPHTWIKELKPENLKKILNSPIKKDIGVF
HHHHHCCHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHCCHHHHHHHHCCHHHHHHHHH
VYKSMLKDLDDDRYLVETIAIDGDFQQHFYVEIHKRNKGWLVKLDSHGLPFRTPAVKKAV
HHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCEEEEECCCCCCCCCHHHHHHH
YELGKILEKRRGAL
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]