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The map label for this gene is topB [H]

Identifier: 238925912

GI number: 238925912

Start: 3342478

End: 3344550

Strand: Reverse

Name: topB [H]

Synonym: EUBREC_3570

Alternate gene names: 238925912

Gene position: 3344550-3342478 (Counterclockwise)

Preceding gene: 238925913

Following gene: 238925911

Centisome position: 96.95

GC content: 43.32

Gene sequence:

>2073_bases
ATGAAATTAGTGATTGCAGAAAAGCCATCGGTTGCGATGGCACTGGCTTCCGTGATTGGAGCCAGAACAAGAAAAGATGG
CTACGTGGAAGGAAACGGTTATCTCGTTTCCTGGTGCGTAGGACATCTGGTTGGATTATGTGATGCATCAGAATACGATG
AAAAATATAAAAAATGGAGATATGAAGATCTGCCGATTGTGCCGGAATGTTGGAAACACAGGGTATTGGAAGGCACGAAA
AAGCAGTTTGGAATCTTGAAAAAACTGATGCGAGATTCCAAAGTAGATGAAGTAATCTGTGCTACAGATGCCGGGCGGGA
AGGAGAACTAATCTTCCGTCTGGTGTATGAGCAGGCAGGATGCAAAAAGCCGATGAAGCGGCTCTGGATCTCTTCCATGG
AGGAACAGGCAATTAAAGATGGATTTGCGTCATTAAAAGACGGTTCGTGTTATGACAGTCTCTATCAGTCAGCACTTTGC
AGGGCAAAGGCAGACTGGCTGGTGGGAATCAATGCGAGCCGTCTTTTTTCCGTTCTGTATAACCAGAATCTGAAAGTTGG
AAGAGTACAGACACCAACACTTGCCATGATCGTGGATCGAAATCAGAAGATCAAGGAATTTACCAAAGAAAAATATTATA
TGGCTCATATCAAGTTTGACGAGATGGATGCGGTCACAGAGCATTTTCAGAAAAAAGAAGATGCAGATAGAGTGGCTGCG
GACTGTATGGAGCGTATGTGCGAAGTGGAAAAGGATGATGTGAAAGAAAAAACGGTCCGTCCTCCGAAGCTCTATGATTT
GACCACTCTGCAGAGGGAAGCAAACAGAATGTTTGGTTATACCGCACAGCAGACATTGGATGCCGTGCAGGAGATGTATG
AGCAGAAGCTGGTCACTTATCCGAGAACAGACAGCCAGTATCTGACAGATGAGATGGGAGAAAGCACTGAAACACTGATT
CAGATGTTACTTGGGAAAATGCCTTATGCTGAAGGTCTGGAATATCACCCAGACGTGAGTAAGGTATTAAATTCAAAGAA
AGTATCCGATCACCATGCGATTATCCCAACCATGGAAGTAGCCAAAGCAGATATTGGTGAACTGAAAGAACGAAACCGCA
AGATTTTGTATCTGATCAGTGCCAGGGTTCTGACAGCAACTGCAGATCCTTATATTTATGAAAGCCATAAATGCCAGATC
ACCTGCAATTATCATACGTTTTATCTCACAGCAAAGAAAACAAAGCAGGAAGGATTCAAGGCGATCGAAAATAAATTGAA
ACAGTTCTTTGGCGTAAAGATTGAGAAAGAAGAGCCGGAGCTGGATATCTGGGCAGGCAAGCATTATGGTCCCTGTGATT
CTTTGGTATCGGAGCATTTCACACAGCCACCGAAACAGTACACAGAAGATACCCTGCTTTCTGCGATGGAACGTGCAGGG
AATGAAGAACTGACCGAAGATACAGAAAAGAAAGGACTTGGTACTCCGGCAACAAGGGCAGCAATCATTGAAAAACTGAT
TCAGTCTGGTTTTGTGAAAAGGGAAAAGAAAAATCTGGTGCCGACAGATGATGGTAATGTATTGATTACAGTTCTGCCAG
ATGAGATTAAATCTCCGAAGATGACCGCAGAGTGGGAAATGGCATTGAATCACATTGCCCAGAATACAGAGACAGCAGAT
GAATTCCTAAATGGGATTACAGAACTGATGCAGGAACTGGTTGCCAGGTATCAGGGAATTTCAGAAGAAAAGAAAAATCA
GTTTCAGGGAAAGGCAAAAGGAGAAGTCATTGGCAAATGTCCCCGATGTGGAGCAGATGTCCAGGAAGGGAAAGTAAACT
TTTACTGTTCTGACCGGAACTGTACTTTTACCTTATGGAAGAATGATAAATTCCTTGCGAGTCAGGGAAAAAAGATGGAT
AAGACGGCAGCAAAGAAGTTCCTGTCTAAAGGAAAAATCCATTATAAGGATCTGGTATCAAGAAAAACAGGGAGACAGTA
CGAAGCAACTGTGGAGATGGTTGATCCCGGAGAGGGAAATGTACAGTTCAATCTGATTTTTCCACAACGATAA

Upstream 100 bases:

>100_bases
CGATGCAGATGAGCAGGAAGAAAGTATAGAACCAGAAATCCTGAGAGACGAGGATTTTGATGATGCTGTGCTGGAAGATG
AAGAAGAGGAGCAGGAGTAA

Downstream 100 bases:

>100_bases
GTAGTGTATTGCAGGCAGTCTAAGGACTGTCTGTTTTAATACAAATATATGAAAGAGAGGACCTTAGCATGAAGAAAAAA
GGTAATTTTAGAAAAGTAGT

Product: DNA topoisomerase

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 690; Mature: 690

Protein sequence:

>690_residues
MKLVIAEKPSVAMALASVIGARTRKDGYVEGNGYLVSWCVGHLVGLCDASEYDEKYKKWRYEDLPIVPECWKHRVLEGTK
KQFGILKKLMRDSKVDEVICATDAGREGELIFRLVYEQAGCKKPMKRLWISSMEEQAIKDGFASLKDGSCYDSLYQSALC
RAKADWLVGINASRLFSVLYNQNLKVGRVQTPTLAMIVDRNQKIKEFTKEKYYMAHIKFDEMDAVTEHFQKKEDADRVAA
DCMERMCEVEKDDVKEKTVRPPKLYDLTTLQREANRMFGYTAQQTLDAVQEMYEQKLVTYPRTDSQYLTDEMGESTETLI
QMLLGKMPYAEGLEYHPDVSKVLNSKKVSDHHAIIPTMEVAKADIGELKERNRKILYLISARVLTATADPYIYESHKCQI
TCNYHTFYLTAKKTKQEGFKAIENKLKQFFGVKIEKEEPELDIWAGKHYGPCDSLVSEHFTQPPKQYTEDTLLSAMERAG
NEELTEDTEKKGLGTPATRAAIIEKLIQSGFVKREKKNLVPTDDGNVLITVLPDEIKSPKMTAEWEMALNHIAQNTETAD
EFLNGITELMQELVARYQGISEEKKNQFQGKAKGEVIGKCPRCGADVQEGKVNFYCSDRNCTFTLWKNDKFLASQGKKMD
KTAAKKFLSKGKIHYKDLVSRKTGRQYEATVEMVDPGEGNVQFNLIFPQR

Sequences:

>Translated_690_residues
MKLVIAEKPSVAMALASVIGARTRKDGYVEGNGYLVSWCVGHLVGLCDASEYDEKYKKWRYEDLPIVPECWKHRVLEGTK
KQFGILKKLMRDSKVDEVICATDAGREGELIFRLVYEQAGCKKPMKRLWISSMEEQAIKDGFASLKDGSCYDSLYQSALC
RAKADWLVGINASRLFSVLYNQNLKVGRVQTPTLAMIVDRNQKIKEFTKEKYYMAHIKFDEMDAVTEHFQKKEDADRVAA
DCMERMCEVEKDDVKEKTVRPPKLYDLTTLQREANRMFGYTAQQTLDAVQEMYEQKLVTYPRTDSQYLTDEMGESTETLI
QMLLGKMPYAEGLEYHPDVSKVLNSKKVSDHHAIIPTMEVAKADIGELKERNRKILYLISARVLTATADPYIYESHKCQI
TCNYHTFYLTAKKTKQEGFKAIENKLKQFFGVKIEKEEPELDIWAGKHYGPCDSLVSEHFTQPPKQYTEDTLLSAMERAG
NEELTEDTEKKGLGTPATRAAIIEKLIQSGFVKREKKNLVPTDDGNVLITVLPDEIKSPKMTAEWEMALNHIAQNTETAD
EFLNGITELMQELVARYQGISEEKKNQFQGKAKGEVIGKCPRCGADVQEGKVNFYCSDRNCTFTLWKNDKFLASQGKKMD
KTAAKKFLSKGKIHYKDLVSRKTGRQYEATVEMVDPGEGNVQFNLIFPQR
>Mature_690_residues
MKLVIAEKPSVAMALASVIGARTRKDGYVEGNGYLVSWCVGHLVGLCDASEYDEKYKKWRYEDLPIVPECWKHRVLEGTK
KQFGILKKLMRDSKVDEVICATDAGREGELIFRLVYEQAGCKKPMKRLWISSMEEQAIKDGFASLKDGSCYDSLYQSALC
RAKADWLVGINASRLFSVLYNQNLKVGRVQTPTLAMIVDRNQKIKEFTKEKYYMAHIKFDEMDAVTEHFQKKEDADRVAA
DCMERMCEVEKDDVKEKTVRPPKLYDLTTLQREANRMFGYTAQQTLDAVQEMYEQKLVTYPRTDSQYLTDEMGESTETLI
QMLLGKMPYAEGLEYHPDVSKVLNSKKVSDHHAIIPTMEVAKADIGELKERNRKILYLISARVLTATADPYIYESHKCQI
TCNYHTFYLTAKKTKQEGFKAIENKLKQFFGVKIEKEEPELDIWAGKHYGPCDSLVSEHFTQPPKQYTEDTLLSAMERAG
NEELTEDTEKKGLGTPATRAAIIEKLIQSGFVKREKKNLVPTDDGNVLITVLPDEIKSPKMTAEWEMALNHIAQNTETAD
EFLNGITELMQELVARYQGISEEKKNQFQGKAKGEVIGKCPRCGADVQEGKVNFYCSDRNCTFTLWKNDKFLASQGKKMD
KTAAKKFLSKGKIHYKDLVSRKTGRQYEATVEMVDPGEGNVQFNLIFPQR

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=699, Percent_Identity=22.7467811158798, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI10835218, Length=344, Percent_Identity=22.6744186046512, Blast_Score=68, Evalue=4e-11,
Organism=Escherichia coli, GI1788061, Length=637, Percent_Identity=32.6530612244898, Blast_Score=280, Evalue=3e-76,
Organism=Escherichia coli, GI1787529, Length=661, Percent_Identity=24.0544629349471, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32563869, Length=610, Percent_Identity=23.6065573770492, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17555378, Length=627, Percent_Identity=20.8931419457735, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 78736; Mature: 78736

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVIAEKPSVAMALASVIGARTRKDGYVEGNGYLVSWCVGHLVGLCDASEYDEKYKKWR
CEEEEECCCHHHHHHHHHHHCCCCCCCEECCCCEEHHHHHHHHHHHCCCHHHHHHHHHCC
YEDLPIVPECWKHRVLEGTKKQFGILKKLMRDSKVDEVICATDAGREGELIFRLVYEQAG
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHC
CKKPMKRLWISSMEEQAIKDGFASLKDGSCYDSLYQSALCRAKADWLVGINASRLFSVLY
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
NQNLKVGRVQTPTLAMIVDRNQKIKEFTKEKYYMAHIKFDEMDAVTEHFQKKEDADRVAA
CCCCCCCEECCCEEEEEECCCHHHHHHHHHCEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
DCMERMCEVEKDDVKEKTVRPPKLYDLTTLQREANRMFGYTAQQTLDAVQEMYEQKLVTY
HHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
PRTDSQYLTDEMGESTETLIQMLLGKMPYAEGLEYHPDVSKVLNSKKVSDHHAIIPTMEV
CCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHH
AKADIGELKERNRKILYLISARVLTATADPYIYESHKCQITCNYHTFYLTAKKTKQEGFK
HHHHHHHHHHCCCEEEEEEEEEHEEECCCCCEEECCEEEEEEEEEEEEEEECHHHHHHHH
AIENKLKQFFGVKIEKEEPELDIWAGKHYGPCDSLVSEHFTQPPKQYTEDTLLSAMERAG
HHHHHHHHHHCCEEECCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
NEELTEDTEKKGLGTPATRAAIIEKLIQSGFVKREKKNLVPTDDGNVLITVLPDEIKSPK
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCC
MTAEWEMALNHIAQNTETADEFLNGITELMQELVARYQGISEEKKNQFQGKAKGEVIGKC
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHCCC
PRCGADVQEGKVNFYCSDRNCTFTLWKNDKFLASQGKKMDKTAAKKFLSKGKIHYKDLVS
CCCCCCCCCCCEEEEECCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHH
RKTGRQYEATVEMVDPGEGNVQFNLIFPQR
HHCCCCEEEEEEEECCCCCCEEEEEECCCC
>Mature Secondary Structure
MKLVIAEKPSVAMALASVIGARTRKDGYVEGNGYLVSWCVGHLVGLCDASEYDEKYKKWR
CEEEEECCCHHHHHHHHHHHCCCCCCCEECCCCEEHHHHHHHHHHHCCCHHHHHHHHHCC
YEDLPIVPECWKHRVLEGTKKQFGILKKLMRDSKVDEVICATDAGREGELIFRLVYEQAG
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHC
CKKPMKRLWISSMEEQAIKDGFASLKDGSCYDSLYQSALCRAKADWLVGINASRLFSVLY
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
NQNLKVGRVQTPTLAMIVDRNQKIKEFTKEKYYMAHIKFDEMDAVTEHFQKKEDADRVAA
CCCCCCCEECCCEEEEEECCCHHHHHHHHHCEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
DCMERMCEVEKDDVKEKTVRPPKLYDLTTLQREANRMFGYTAQQTLDAVQEMYEQKLVTY
HHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
PRTDSQYLTDEMGESTETLIQMLLGKMPYAEGLEYHPDVSKVLNSKKVSDHHAIIPTMEV
CCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHH
AKADIGELKERNRKILYLISARVLTATADPYIYESHKCQITCNYHTFYLTAKKTKQEGFK
HHHHHHHHHHCCCEEEEEEEEEHEEECCCCCEEECCEEEEEEEEEEEEEEECHHHHHHHH
AIENKLKQFFGVKIEKEEPELDIWAGKHYGPCDSLVSEHFTQPPKQYTEDTLLSAMERAG
HHHHHHHHHHCCEEECCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
NEELTEDTEKKGLGTPATRAAIIEKLIQSGFVKREKKNLVPTDDGNVLITVLPDEIKSPK
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCC
MTAEWEMALNHIAQNTETADEFLNGITELMQELVARYQGISEEKKNQFQGKAKGEVIGKC
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHCCC
PRCGADVQEGKVNFYCSDRNCTFTLWKNDKFLASQGKKMDKTAAKKFLSKGKIHYKDLVS
CCCCCCCCCCCEEEEECCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHH
RKTGRQYEATVEMVDPGEGNVQFNLIFPQR
HHCCCCEEEEEEEECCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA