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The map label for this gene is vanS [H]

Identifier: 238925900

GI number: 238925900

Start: 3320029

End: 3321114

Strand: Reverse

Name: vanS [H]

Synonym: EUBREC_3558

Alternate gene names: 238925900

Gene position: 3321114-3320029 (Counterclockwise)

Preceding gene: 238925901

Following gene: 238925899

Centisome position: 96.27

GC content: 38.31

Gene sequence:

>1086_bases
ATGAAATTGAATAAAAAAGAAAAAAACTATCTGCTCTCTACCTTGTTCAAAGGGTATGTAATTCAATGTGCGATTTGCTC
TGTGCTTATTTTTGGCGGTACTTTTCTTTTAGGAATTATGGCAGAGGAAATTCTCCGCAAGTATGTTTTGTATTATTACT
TGTATTACAGAACAGTATATCTTTATATAGTGGCTGTAATCGTGTGGGGTGGCTGTATTATATACATGACATACCTACTT
TTGAAAAAAGTAGTCGCCTATGTGTACGAGGTACAGGCTGCGACAGGAAAAATGTTCGATCAGAATGTCAGTTACATTGA
AATGTCCCCGGAGTTGAGTGAAATCGCCGCTAACATCAATCAGTTAAAACAAGAGGCTGAAAGTAATGCAAGGCTTGCAA
AAGAAAATGAGCAGCGAAAAAATGACCTGATTATGTATCTTGCACACGATTTGAAAACACCGCTTTCTTCTGTAATCGGG
TATCTTACTTTACTGCGTGATGAGTCGCAAATCTCAAAGGAACTTCGAGAAAAGTACCTTTCAATCACTCTTGGTAAGGC
AGAACGGTTAGAGGATCTGATTAACGAGTTTTTCGAGATAACACGATTCAATATATACGATATTACATTACAATACACGA
AAATCAATTTAACTCGCCTGCTGGAGCAACTTGTATATGAATTTAAGCCTATGCTCAAATCAAAGAATTTGCAATGTAAT
CTTTGTGTTGATGATGATATAATGCTTCGATGTGACGCAGACAAAATTCAGCGTGTTTTTGATAACCTTTTGAGAAATGC
GGTTATCTACAGTTTTGAAAATACGGATATCACGATCAGCGCACAATGTCAGGAAGATACGGTCAGCATTATTTTTTGTA
ACCACGGCGATACTCTGCCGGAAGAAAAGTTAAACAGGATTTTCGAGCAGTTTTATCGGCTTGACGCAGCGAGAAGTACA
AGCAGTGGAGGAGCTGGATTAGGTTTGGCGATTGCAAAGCAGATTGTTGAACTACACAATGGTACAATCGTTGCAGAGAG
CCAAGAGGATCAAAACAAGTTTTCAATTACTTTGCCGTTGGCGTAG

Upstream 100 bases:

>100_bases
AGTAACAATACGGTAATGGCGCACATTGGGCGGCTTCGGGAAAAATTGAATGAACCTTCAAAAAATCCGAAGTTCATTAA
AACTGTTTGGGGGGTAGGGT

Downstream 100 bases:

>100_bases
GAAAATTGTAAGAATTTGCATAGAAAAAAAGAGGAATACCTTACGAGCAATCAAGAAAATACGGGCTTCTGTTTTGGTAC
AATAAAAGTATCAAGATAGA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKLNKKEKNYLLSTLFKGYVIQCAICSVLIFGGTFLLGIMAEEILRKYVLYYYLYYRTVYLYIVAVIVWGGCIIYMTYLL
LKKVVAYVYEVQAATGKMFDQNVSYIEMSPELSEIAANINQLKQEAESNARLAKENEQRKNDLIMYLAHDLKTPLSSVIG
YLTLLRDESQISKELREKYLSITLGKAERLEDLINEFFEITRFNIYDITLQYTKINLTRLLEQLVYEFKPMLKSKNLQCN
LCVDDDIMLRCDADKIQRVFDNLLRNAVIYSFENTDITISAQCQEDTVSIIFCNHGDTLPEEKLNRIFEQFYRLDAARST
SSGGAGLGLAIAKQIVELHNGTIVAESQEDQNKFSITLPLA

Sequences:

>Translated_361_residues
MKLNKKEKNYLLSTLFKGYVIQCAICSVLIFGGTFLLGIMAEEILRKYVLYYYLYYRTVYLYIVAVIVWGGCIIYMTYLL
LKKVVAYVYEVQAATGKMFDQNVSYIEMSPELSEIAANINQLKQEAESNARLAKENEQRKNDLIMYLAHDLKTPLSSVIG
YLTLLRDESQISKELREKYLSITLGKAERLEDLINEFFEITRFNIYDITLQYTKINLTRLLEQLVYEFKPMLKSKNLQCN
LCVDDDIMLRCDADKIQRVFDNLLRNAVIYSFENTDITISAQCQEDTVSIIFCNHGDTLPEEKLNRIFEQFYRLDAARST
SSGGAGLGLAIAKQIVELHNGTIVAESQEDQNKFSITLPLA
>Mature_361_residues
MKLNKKEKNYLLSTLFKGYVIQCAICSVLIFGGTFLLGIMAEEILRKYVLYYYLYYRTVYLYIVAVIVWGGCIIYMTYLL
LKKVVAYVYEVQAATGKMFDQNVSYIEMSPELSEIAANINQLKQEAESNARLAKENEQRKNDLIMYLAHDLKTPLSSVIG
YLTLLRDESQISKELREKYLSITLGKAERLEDLINEFFEITRFNIYDITLQYTKINLTRLLEQLVYEFKPMLKSKNLQCN
LCVDDDIMLRCDADKIQRVFDNLLRNAVIYSFENTDITISAQCQEDTVSIIFCNHGDTLPEEKLNRIFEQFYRLDAARST
SSGGAGLGLAIAKQIVELHNGTIVAESQEDQNKFSITLPLA

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=251, Percent_Identity=29.8804780876494, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1786912, Length=244, Percent_Identity=29.0983606557377, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=27.6785714285714, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1786783, Length=291, Percent_Identity=24.7422680412371, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=26.7543859649123, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790346, Length=207, Percent_Identity=31.4009661835749, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI87082128, Length=322, Percent_Identity=25.776397515528, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=22.3577235772358, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789149, Length=237, Percent_Identity=26.1603375527426, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787894, Length=322, Percent_Identity=24.5341614906832, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790861, Length=280, Percent_Identity=23.2142857142857, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=25.7918552036199, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788279, Length=218, Percent_Identity=26.1467889908257, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994928, Length=228, Percent_Identity=23.2456140350877, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788549, Length=247, Percent_Identity=24.2914979757085, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081816, Length=255, Percent_Identity=23.921568627451, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787374, Length=306, Percent_Identity=22.8758169934641, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790551, Length=307, Percent_Identity=22.1498371335505, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41543; Mature: 41543

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNKKEKNYLLSTLFKGYVIQCAICSVLIFGGTFLLGIMAEEILRKYVLYYYLYYRTVY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYIVAVIVWGGCIIYMTYLLLKKVVAYVYEVQAATGKMFDQNVSYIEMSPELSEIAANIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHH
QLKQEAESNARLAKENEQRKNDLIMYLAHDLKTPLSSVIGYLTLLRDESQISKELREKYL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
SITLGKAERLEDLINEFFEITRFNIYDITLQYTKINLTRLLEQLVYEFKPMLKSKNLQCN
HHHCCCHHHHHHHHHHHHHHHCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEE
LCVDDDIMLRCDADKIQRVFDNLLRNAVIYSFENTDITISAQCQEDTVSIIFCNHGDTLP
EEECCCEEEEECHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCEEEEEECCCCCCC
EEKLNRIFEQFYRLDAARSTSSGGAGLGLAIAKQIVELHNGTIVAESQEDQNKFSITLPL
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEECC
A
C
>Mature Secondary Structure
MKLNKKEKNYLLSTLFKGYVIQCAICSVLIFGGTFLLGIMAEEILRKYVLYYYLYYRTVY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYIVAVIVWGGCIIYMTYLLLKKVVAYVYEVQAATGKMFDQNVSYIEMSPELSEIAANIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHH
QLKQEAESNARLAKENEQRKNDLIMYLAHDLKTPLSSVIGYLTLLRDESQISKELREKYL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
SITLGKAERLEDLINEFFEITRFNIYDITLQYTKINLTRLLEQLVYEFKPMLKSKNLQCN
HHHCCCHHHHHHHHHHHHHHHCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEE
LCVDDDIMLRCDADKIQRVFDNLLRNAVIYSFENTDITISAQCQEDTVSIIFCNHGDTLP
EEECCCEEEEECHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCEEEEEECCCCCCC
EEKLNRIFEQFYRLDAARSTSSGGAGLGLAIAKQIVELHNGTIVAESQEDQNKFSITLPL
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEECC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]