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The map label for this gene is vanS [H]

Identifier: 238925848

GI number: 238925848

Start: 3280958

End: 3282022

Strand: Direct

Name: vanS [H]

Synonym: EUBREC_3506

Alternate gene names: 238925848

Gene position: 3280958-3282022 (Clockwise)

Preceding gene: 238925847

Following gene: 238925849

Centisome position: 95.11

GC content: 40.19

Gene sequence:

>1065_bases
ATGTCGAAGAAAGAAATGACAACAGAAAAGCGCCTTAAAGTGCGCTTATACCTATCATTGCTGGTTTATACCGTTGTTGG
ATATGGTTTGACCCTTATCTTGGACTACATCTTTTCAAAATTTGACAACGGTATTTTTGCATGGCTCTACTGGCGGCTCG
ATCTGCTTTTTATTTTGTATCTGATGATCGGCTTTGTATGTATTTTCAATTACTATTGGAAAAAGCCGTGGGGCTATCTG
GATGAAGTAATTGATGCAACCCAAACGGTCTATGAGCAGAACAATCATACCGTATCATTGTCTGACCCACTGAAAGAATT
GGAGGCACAACTCAATCAAATCAAAATGTCTGTTCTGTTGAGCAAACGGGCTGCAAAGCAGGCAGAAGAAAAGAAAAATG
AAATTATTATGTATCTGGCACATGATATACGCACCCCTCTCACAACGGTAATCGGCTATTTGAGCCTGCTCCATGAAGCC
CCTGACATGCCTGAACAGCAAAAGGAAAAGTATGTAAAAGTTGCGTTGAATAAGGCAGAACGCCTTGAAAAGCTCATCAA
TGAACTTTTTGAAATTACCAAGTACAATGCACATACGGTCATTATCAAAAAAGAAAATATAGATTTACATTGTTTGATTG
CGCAGGTAATAGATGAAATATACCCGACACTCTCTGCAAATGGCAACACGGCGGTATTTACTGCGGAGGACAACTTATCT
GTGAACGCTGATCCCGAAAAGCTGGCGAGAGTTTTCAGTAACCTTTTACGAAATGCAGCGTCATATAGCTACCCGCAGAC
AGAGATTACTATATCCGCCAAACGGTTAGAGCATGATATACAGATCACTTTTGAAAATCGTGGAAAAACAATTCCACAGG
AACAGCTTAACAGTATATTTGAAAAATTCAATCGCTTGGATGATGCTCGGCTTTCTAATACAGGTGGTGCGGGGCTTGGC
CTTTCTATTGCGGAAGAAATTATACATTTACATGGTGGAAAGATTACTGCATCCAGTCAAAATGAAACCATAGACTTTGT
GATTACATTACCCCTTTCCGCTTAG

Upstream 100 bases:

>100_bases
GCAAACAGACTGTTTTCCATTCTGGCAGGCATAGTAAGTGTTGTCGTGATTGGCCTACTTGGCTGTCTTCTGTTTGCTAA
TCGGTAGGAGGTGTATTCTC

Downstream 100 bases:

>100_bases
GAAAATAAGAATGTGATCTTAGGAATTACTTAGGAATTTAAGCGTGTGATTTTTGAGATTGAAAAGCCTTTGTTCGGTAC
AATGATATCGAACAAGGGCT

Product: VanS

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSKKEMTTEKRLKVRLYLSLLVYTVVGYGLTLILDYIFSKFDNGIFAWLYWRLDLLFILYLMIGFVCIFNYYWKKPWGYL
DEVIDATQTVYEQNNHTVSLSDPLKELEAQLNQIKMSVLLSKRAAKQAEEKKNEIIMYLAHDIRTPLTTVIGYLSLLHEA
PDMPEQQKEKYVKVALNKAERLEKLINELFEITKYNAHTVIIKKENIDLHCLIAQVIDEIYPTLSANGNTAVFTAEDNLS
VNADPEKLARVFSNLLRNAASYSYPQTEITISAKRLEHDIQITFENRGKTIPQEQLNSIFEKFNRLDDARLSNTGGAGLG
LSIAEEIIHLHGGKITASSQNETIDFVITLPLSA

Sequences:

>Translated_354_residues
MSKKEMTTEKRLKVRLYLSLLVYTVVGYGLTLILDYIFSKFDNGIFAWLYWRLDLLFILYLMIGFVCIFNYYWKKPWGYL
DEVIDATQTVYEQNNHTVSLSDPLKELEAQLNQIKMSVLLSKRAAKQAEEKKNEIIMYLAHDIRTPLTTVIGYLSLLHEA
PDMPEQQKEKYVKVALNKAERLEKLINELFEITKYNAHTVIIKKENIDLHCLIAQVIDEIYPTLSANGNTAVFTAEDNLS
VNADPEKLARVFSNLLRNAASYSYPQTEITISAKRLEHDIQITFENRGKTIPQEQLNSIFEKFNRLDDARLSNTGGAGLG
LSIAEEIIHLHGGKITASSQNETIDFVITLPLSA
>Mature_353_residues
SKKEMTTEKRLKVRLYLSLLVYTVVGYGLTLILDYIFSKFDNGIFAWLYWRLDLLFILYLMIGFVCIFNYYWKKPWGYLD
EVIDATQTVYEQNNHTVSLSDPLKELEAQLNQIKMSVLLSKRAAKQAEEKKNEIIMYLAHDIRTPLTTVIGYLSLLHEAP
DMPEQQKEKYVKVALNKAERLEKLINELFEITKYNAHTVIIKKENIDLHCLIAQVIDEIYPTLSANGNTAVFTAEDNLSV
NADPEKLARVFSNLLRNAASYSYPQTEITISAKRLEHDIQITFENRGKTIPQEQLNSIFEKFNRLDDARLSNTGGAGLGL
SIAEEIIHLHGGKITASSQNETIDFVITLPLSA

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=28.8888888888889, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786783, Length=215, Percent_Identity=29.3023255813954, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1786912, Length=235, Percent_Identity=31.9148936170213, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788393, Length=259, Percent_Identity=25.4826254826255, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790436, Length=244, Percent_Identity=28.6885245901639, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790346, Length=234, Percent_Identity=28.2051282051282, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI145693157, Length=247, Percent_Identity=25.5060728744939, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788713, Length=241, Percent_Identity=24.4813278008299, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789149, Length=254, Percent_Identity=24.8031496062992, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082128, Length=309, Percent_Identity=26.2135922330097, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=26.4957264957265, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87081816, Length=268, Percent_Identity=23.8805970149254, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790861, Length=298, Percent_Identity=24.8322147651007, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788279, Length=215, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788549, Length=239, Percent_Identity=27.6150627615063, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790300, Length=245, Percent_Identity=25.3061224489796, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787894, Length=226, Percent_Identity=27.8761061946903, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787374, Length=321, Percent_Identity=23.0529595015576, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40491; Mature: 40360

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKEMTTEKRLKVRLYLSLLVYTVVGYGLTLILDYIFSKFDNGIFAWLYWRLDLLFILY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LMIGFVCIFNYYWKKPWGYLDEVIDATQTVYEQNNHTVSLSDPLKELEAQLNQIKMSVLL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHH
SKRAAKQAEEKKNEIIMYLAHDIRTPLTTVIGYLSLLHEAPDMPEQQKEKYVKVALNKAE
HHHHHHHHHHHHCCEEEEEHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RLEKLINELFEITKYNAHTVIIKKENIDLHCLIAQVIDEIYPTLSANGNTAVFTAEDNLS
HHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
VNADPEKLARVFSNLLRNAASYSYPQTEITISAKRLEHDIQITFENRGKTIPQEQLNSIF
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHCCEEEEEECCCCCCCCHHHHHHHH
EKFNRLDDARLSNTGGAGLGLSIAEEIIHLHGGKITASSQNETIDFVITLPLSA
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCC
>Mature Secondary Structure 
SKKEMTTEKRLKVRLYLSLLVYTVVGYGLTLILDYIFSKFDNGIFAWLYWRLDLLFILY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LMIGFVCIFNYYWKKPWGYLDEVIDATQTVYEQNNHTVSLSDPLKELEAQLNQIKMSVLL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHH
SKRAAKQAEEKKNEIIMYLAHDIRTPLTTVIGYLSLLHEAPDMPEQQKEKYVKVALNKAE
HHHHHHHHHHHHCCEEEEEHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RLEKLINELFEITKYNAHTVIIKKENIDLHCLIAQVIDEIYPTLSANGNTAVFTAEDNLS
HHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
VNADPEKLARVFSNLLRNAASYSYPQTEITISAKRLEHDIQITFENRGKTIPQEQLNSIF
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHCCEEEEEECCCCCCCCHHHHHHHH
EKFNRLDDARLSNTGGAGLGLSIAEEIIHLHGGKITASSQNETIDFVITLPLSA
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]