BacMap

Definition	Eubacterium rectale ATCC 33656, complete genome.
Accession	NC_012781
Length	3,449,685

Click here to switch to the map view.

The map label for this gene is yqhE [C]

Identifier: 238925803

GI number: 238925803

Start: 3250846

End: 3251685

Strand: Reverse

Name: yqhE [C]

Synonym: EUBREC_3461

Alternate gene names: 238925803

Gene position: 3251685-3250846 (Counterclockwise)

Preceding gene: 238925804

Following gene: 238925802

Centisome position: 94.26

GC content: 36.31

Gene sequence:

>840_bases
ATGGAAAATATGAAGTTGAATAACAATTTGGAATGTCCAATGCTTGGACTAGGCACTTTTATGTTATCACCAGAGGATGC
CTACACAAGTACATTAGAGGCATTAAAAATGGGATATTCCCTAATTGATACAGCAAATGCTTATGTAAATGAGCGTGCAG
TTGGAAGAGCAATCAAGGATAGTGGAATTGACAGAAAAAATATATTTCTATCAACAAAAATATGGGCTAGTGAATATGAA
AATGAGAATGCTGTAGAAGAAACTCTAGAAAGACTTGGTGTTGATTATGTGGATTTACTTTATATACACCAGCCTGCAGG
CAACTGGCTTGCCGGATACAGAATGCTTGAAAAAGCGTATAGAGAGGGCAAAGCAAAATCCATTGGTATCTCGAATTTTG
AAGGAAAATATATGGAAGAGTTGGAAACAAAGTGGGAAATTGTACCTCAGTTTATTCAGGTGGAAGCACATCCATATTTC
ACACAGAAAGAACTTCGTGTAACTTTAGACAAATATGGAATTAAGCTTATGAGCTGGTATCCGTTGGGACATGGAGATAC
AGCATTAATGAATGAGTTGGTATTTGCAGGACTTGGCAAAAAATATGGAAAAACACCTGCACAGGTTATTCTCCGTTGGC
ATACTCAGATGGGATTTGTTGTAATTCCTGGAAGTAAAAATGCAGAACACATTAAGGACAATATGAATATCTTTGATTTT
ACATTAACAGATGAAGAAATGGAACAGATTGCAAAGCTGGACAAGAATGAAAGATATTATCACCGCATGGATGAACAGCT
TGTACAGTTTGCAAACTGGAAACCAGAGTTTGAAAAATAA

Upstream 100 bases:

>100_bases
TATGAGGAAAAGGATGTACCGGATTATGCAGTAGCAGTTGGAAATCCGGCAAAAGTAATAAAAATGCTTGATAAAGAAAA
ATTTCAGGAGGACTAAAAAG

Downstream 100 bases:

>100_bases
TACGGAGCAACTGCTTCCATCTTTTTATCTAGAAGTAGCAGTAAGTAAATCCGAGTAAAATATGGTCAAAAAATCATCGA
TATCCGACCTACAGCACGTT

Product: oxidoreductase, aldo/keto reductase family

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MENMKLNNNLECPMLGLGTFMLSPEDAYTSTLEALKMGYSLIDTANAYVNERAVGRAIKDSGIDRKNIFLSTKIWASEYE
NENAVEETLERLGVDYVDLLYIHQPAGNWLAGYRMLEKAYREGKAKSIGISNFEGKYMEELETKWEIVPQFIQVEAHPYF
TQKELRVTLDKYGIKLMSWYPLGHGDTALMNELVFAGLGKKYGKTPAQVILRWHTQMGFVVIPGSKNAEHIKDNMNIFDF
TLTDEEMEQIAKLDKNERYYHRMDEQLVQFANWKPEFEK

Sequences:

>Translated_279_residues
MENMKLNNNLECPMLGLGTFMLSPEDAYTSTLEALKMGYSLIDTANAYVNERAVGRAIKDSGIDRKNIFLSTKIWASEYE
NENAVEETLERLGVDYVDLLYIHQPAGNWLAGYRMLEKAYREGKAKSIGISNFEGKYMEELETKWEIVPQFIQVEAHPYF
TQKELRVTLDKYGIKLMSWYPLGHGDTALMNELVFAGLGKKYGKTPAQVILRWHTQMGFVVIPGSKNAEHIKDNMNIFDF
TLTDEEMEQIAKLDKNERYYHRMDEQLVQFANWKPEFEK
>Mature_279_residues
MENMKLNNNLECPMLGLGTFMLSPEDAYTSTLEALKMGYSLIDTANAYVNERAVGRAIKDSGIDRKNIFLSTKIWASEYE
NENAVEETLERLGVDYVDLLYIHQPAGNWLAGYRMLEKAYREGKAKSIGISNFEGKYMEELETKWEIVPQFIQVEAHPYF
TQKELRVTLDKYGIKLMSWYPLGHGDTALMNELVFAGLGKKYGKTPAQVILRWHTQMGFVVIPGSKNAEHIKDNMNIFDF
TLTDEEMEQIAKLDKNERYYHRMDEQLVQFANWKPEFEK

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=295, Percent_Identity=30.5084745762712, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI5174391, Length=295, Percent_Identity=30.5084745762712, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI310109922, Length=279, Percent_Identity=35.1254480286738, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI5174695, Length=299, Percent_Identity=35.1170568561873, Blast_Score=157, Evalue=7e-39,
Organism=Homo sapiens, GI45446745, Length=301, Percent_Identity=33.5548172757475, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI4503285, Length=301, Percent_Identity=33.5548172757475, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI310109920, Length=300, Percent_Identity=33, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI5453543, Length=300, Percent_Identity=33, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI24497583, Length=303, Percent_Identity=32.013201320132, Blast_Score=147, Evalue=7e-36,
Organism=Homo sapiens, GI93277124, Length=297, Percent_Identity=29.96632996633, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI24497585, Length=301, Percent_Identity=31.2292358803987, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI300116271, Length=279, Percent_Identity=34.7670250896057, Blast_Score=140, Evalue=9e-34,
Organism=Homo sapiens, GI223468663, Length=293, Percent_Identity=30.7167235494881, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=32.8519855595668, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4502049, Length=291, Percent_Identity=31.9587628865979, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI291291012, Length=265, Percent_Identity=31.3207547169811, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=36.7741935483871, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI207028673, Length=117, Percent_Identity=36.7521367521368, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI310109926, Length=116, Percent_Identity=35.3448275862069, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI87082198, Length=259, Percent_Identity=35.9073359073359, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1786400, Length=259, Percent_Identity=35.5212355212355, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788081, Length=286, Percent_Identity=25.5244755244755, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081735, Length=294, Percent_Identity=26.1904761904762, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787674, Length=271, Percent_Identity=27.3062730627306, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17537077, Length=295, Percent_Identity=34.2372881355932, Blast_Score=175, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17537075, Length=295, Percent_Identity=32.8813559322034, Blast_Score=167, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI71998625, Length=266, Percent_Identity=34.5864661654135, Blast_Score=165, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17564128, Length=304, Percent_Identity=32.2368421052632, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17550248, Length=294, Percent_Identity=31.6326530612245, Blast_Score=144, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17561298, Length=277, Percent_Identity=29.6028880866426, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17566692, Length=279, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17538386, Length=278, Percent_Identity=32.3741007194245, Blast_Score=138, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17537079, Length=300, Percent_Identity=30.3333333333333, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17561300, Length=275, Percent_Identity=30.1818181818182, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17562292, Length=283, Percent_Identity=32.5088339222615, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17552492, Length=269, Percent_Identity=28.996282527881, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6324694, Length=304, Percent_Identity=35.5263157894737, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6320576, Length=284, Percent_Identity=34.8591549295775, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321896, Length=308, Percent_Identity=31.4935064935065, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6319625, Length=315, Percent_Identity=33.3333333333333, Blast_Score=136, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322556, Length=268, Percent_Identity=31.3432835820896, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320079, Length=241, Percent_Identity=31.9502074688797, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45553081, Length=290, Percent_Identity=33.1034482758621, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=33.9160839160839, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24663317, Length=290, Percent_Identity=33.448275862069, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24644950, Length=303, Percent_Identity=32.6732673267327, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24662789, Length=296, Percent_Identity=30.7432432432432, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24657054, Length=293, Percent_Identity=32.0819112627986, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI221378297, Length=323, Percent_Identity=30.6501547987616, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI21356425, Length=290, Percent_Identity=32.0689655172414, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI281366140, Length=256, Percent_Identity=32.03125, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24662781, Length=295, Percent_Identity=32.5423728813559, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24662785, Length=285, Percent_Identity=32.6315789473684, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 32298; Mature: 32298

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENMKLNNNLECPMLGLGTFMLSPEDAYTSTLEALKMGYSLIDTANAYVNERAVGRAIKD
CCCCCCCCCCCCCEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGIDRKNIFLSTKIWASEYENENAVEETLERLGVDYVDLLYIHQPAGNWLAGYRMLEKAY
CCCCCHHEEEEEEHHHHHCCCCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHH
REGKAKSIGISNFEGKYMEELETKWEIVPQFIQVEAHPYFTQKELRVTLDKYGIKLMSWY
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCEEEEEEE
PLGHGDTALMNELVFAGLGKKYGKTPAQVILRWHTQMGFVVIPGSKNAEHIKDNMNIFDF
ECCCCCHHHHHHHHHHHCCHHHCCCHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCEEEE
TLTDEEMEQIAKLDKNERYYHRMDEQLVQFANWKPEFEK
EECHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MENMKLNNNLECPMLGLGTFMLSPEDAYTSTLEALKMGYSLIDTANAYVNERAVGRAIKD
CCCCCCCCCCCCCEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGIDRKNIFLSTKIWASEYENENAVEETLERLGVDYVDLLYIHQPAGNWLAGYRMLEKAY
CCCCCHHEEEEEEHHHHHCCCCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHH
REGKAKSIGISNFEGKYMEELETKWEIVPQFIQVEAHPYFTQKELRVTLDKYGIKLMSWY
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCEEEEEEE
PLGHGDTALMNELVFAGLGKKYGKTPAQVILRWHTQMGFVVIPGSKNAEHIKDNMNIFDF
ECCCCCHHHHHHHHHHHCCHHHCCCHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCEEEE
TLTDEEMEQIAKLDKNERYYHRMDEQLVQFANWKPEFEK
EECHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA