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The map label for this gene is fadH [C]

Identifier: 238925193

GI number: 238925193

Start: 2728518

End: 2730518

Strand: Direct

Name: fadH [C]

Synonym: EUBREC_2847

Alternate gene names: 238925193

Gene position: 2728518-2730518 (Clockwise)

Preceding gene: 238925192

Following gene: 238925195

Centisome position: 79.09

GC content: 45.88

Gene sequence:

>2001_bases
ATGAAAAACAAATATCCACATATTTTTGAGCCAATTACAGTACGAAGAATGACAGTAAAGAACCGTATCGTCATGACTCC
AATGGGAACTAACTACGGTGAGCAGAACGGAGAAATGAGCTTTCTCCACATCAACTACTATGAGCAGAGAGCAAAGGGCG
GCACTGGTCTCATCATCGTTGAGAATGCTAGTGTTGACTCTCCGCAGGGTTCAAACGGAACAACACAGCTTCGTATTGAT
TTGGACAATTACATCCCAAGACTTTTCAAGCTCTGCGAGAGTGTTCACAAGCATGGTGCATGTATCGCGATACAGTTAAA
TCATGCCGGTGCTTCTGCAATGAGCTCACGTATCGGAATGCAGCCTGTATCAGCATCAGACGTACCTTCAAAGGCAGGCG
GAGAAATCCCAAGACCTCTCGAGAAGGATGAAATCATGCATATCGTAAAGAAATACGGTGAGGCAGCCAAGCGTGCCCAG
ATCTGTGGCTTCGACGCTGTTGAAATCCACGCAGGACACTCTTATCTTATCAGTCAGTTCCTTTCACCTATCACAAACAA
GAGAACTGACGAGTTCGGTGGCTCTGCAGAGAACCGTGCAAGATTTGCAAAGCTGGTTATCGAGGAGGTTCGTAAGCAGG
TTGGACCTTTCTTCCCTATTTTCGTCCGTATCAGTGCAGACGAGCTCATGGAGGGTGGAAACACACTTGAGGATACACTT
GAGTACTTAAAGTATTTTGAGAAGGAAGTAGATGTATTCGACGTATCATGTGGTCTCAACGGATCTATCCAGTACCAGAT
TGATGCAAACTACTTACCTGACGGATGGCGCTCATTCATGGCAAAGGCTGTAAAAGAGAAATACAACAAGCCATGTATCA
CAGTAGGAAATATCCGTGACCCACAGGTAGCCGAAGATATCCTTGCAGGAGGCGATGCTGATTTCATCGGTATGGGCCGT
GGACTTATCGCTGACCCTGAGTGGGTTAATAAGGTAGAGTTCGGAAATGTGTGCGATATCAGAAAATGTATCTCATGTAA
CATCGGATGCGCCGGACACAGAATCGGTCTCAACCAGCCAATCCGCTGTACAGTAAACCCTGCCGTAAACAGCGGTGAGG
ATTACATGAAGACAAAGGTAAATAAGCCTTGCAACGTGGTTGTAATCGGTGGTGGTACTGCCGGTCTTGAGGCAGCCTGC
ACAGCAGCAGAGGTTGGATGTACAACCTTCCTCATTGAGAAGAAGGCAGAGCTTGGCGGACTTGCATCAGTTATCTCCAA
GATTCCTGACAAGAAGCGTCTTGCAGACTTCCCTAACTACATGATTCACCGTGCAAGCAAGCTTCACAACCTGTTTGTTT
TCAAAAATACATCAGCAACAGTTGATATGGTAAAGGCCTTAAACCCTGATATCATAGTAAACGCAACAGGCTCTGTACCT
ACTCTCCCACCTATCACAGGTCTTCACGATCTCGTTGACAAGGATGGCACAAATGTTGCAACAGTCCTTAAGATGATTGA
GCGCATCAATGAGTATCCTGAGGATATGAAGGGACAGAAAATCGCCATCATCGGCGGTGGTGCCGTAGGTCTTGACGTGA
TGGAATTCTTCACAGAAAGAGGCGCAGAGGTAACAATGGTTGAGATGCTTCCAATGATTGGAAACGGACTTGACCCTGTC
ACAAAGTGTGACACAAACGCAAAAATGGCAAAATACGGTGTAAAACAGATGACCAACACTGCCCTGCAGGAGGTTAAGAA
TGACCGTTTCATCGTAAAGAATCCAGAGGGTGAGATTGAGGAAATCCCATTTGATTACGGATTCATCTGCCTTGGTATGA
GAGCCAACACACCTGTTCTTTCAGAGATTGATGAGGCCTTCAGTAATACCAATGTTGAAATCGTAAATATCGGAGACAGC
AAGCGTGCCCGCAGAATCATTGAGGGTACAGAGGAAGGTAGAAATATCTTAAACGTGCTTGCCCGTCACGATTATTTATA
A

Upstream 100 bases:

>100_bases
AATATTGACAATCAGTTTAATGAAACAAAACTATAATGTAATGAGAGCAATTTACACTACCGTGTGCCCACCCGGTTAGC
AGTTTAAGGAGAAAAATACT

Downstream 100 bases:

>100_bases
ATTCTCCTTTAAAATAATAAACCTATAAAATCGAGTAGGAGGCGGTGACTAACCGCCGTCCTCTCACAGCACCGTACGTA
CCGTTCGGTATACGGCGCTT

Product: NADH:flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 666; Mature: 666

Protein sequence:

>666_residues
MKNKYPHIFEPITVRRMTVKNRIVMTPMGTNYGEQNGEMSFLHINYYEQRAKGGTGLIIVENASVDSPQGSNGTTQLRID
LDNYIPRLFKLCESVHKHGACIAIQLNHAGASAMSSRIGMQPVSASDVPSKAGGEIPRPLEKDEIMHIVKKYGEAAKRAQ
ICGFDAVEIHAGHSYLISQFLSPITNKRTDEFGGSAENRARFAKLVIEEVRKQVGPFFPIFVRISADELMEGGNTLEDTL
EYLKYFEKEVDVFDVSCGLNGSIQYQIDANYLPDGWRSFMAKAVKEKYNKPCITVGNIRDPQVAEDILAGGDADFIGMGR
GLIADPEWVNKVEFGNVCDIRKCISCNIGCAGHRIGLNQPIRCTVNPAVNSGEDYMKTKVNKPCNVVVIGGGTAGLEAAC
TAAEVGCTTFLIEKKAELGGLASVISKIPDKKRLADFPNYMIHRASKLHNLFVFKNTSATVDMVKALNPDIIVNATGSVP
TLPPITGLHDLVDKDGTNVATVLKMIERINEYPEDMKGQKIAIIGGGAVGLDVMEFFTERGAEVTMVEMLPMIGNGLDPV
TKCDTNAKMAKYGVKQMTNTALQEVKNDRFIVKNPEGEIEEIPFDYGFICLGMRANTPVLSEIDEAFSNTNVEIVNIGDS
KRARRIIEGTEEGRNILNVLARHDYL

Sequences:

>Translated_666_residues
MKNKYPHIFEPITVRRMTVKNRIVMTPMGTNYGEQNGEMSFLHINYYEQRAKGGTGLIIVENASVDSPQGSNGTTQLRID
LDNYIPRLFKLCESVHKHGACIAIQLNHAGASAMSSRIGMQPVSASDVPSKAGGEIPRPLEKDEIMHIVKKYGEAAKRAQ
ICGFDAVEIHAGHSYLISQFLSPITNKRTDEFGGSAENRARFAKLVIEEVRKQVGPFFPIFVRISADELMEGGNTLEDTL
EYLKYFEKEVDVFDVSCGLNGSIQYQIDANYLPDGWRSFMAKAVKEKYNKPCITVGNIRDPQVAEDILAGGDADFIGMGR
GLIADPEWVNKVEFGNVCDIRKCISCNIGCAGHRIGLNQPIRCTVNPAVNSGEDYMKTKVNKPCNVVVIGGGTAGLEAAC
TAAEVGCTTFLIEKKAELGGLASVISKIPDKKRLADFPNYMIHRASKLHNLFVFKNTSATVDMVKALNPDIIVNATGSVP
TLPPITGLHDLVDKDGTNVATVLKMIERINEYPEDMKGQKIAIIGGGAVGLDVMEFFTERGAEVTMVEMLPMIGNGLDPV
TKCDTNAKMAKYGVKQMTNTALQEVKNDRFIVKNPEGEIEEIPFDYGFICLGMRANTPVLSEIDEAFSNTNVEIVNIGDS
KRARRIIEGTEEGRNILNVLARHDYL
>Mature_666_residues
MKNKYPHIFEPITVRRMTVKNRIVMTPMGTNYGEQNGEMSFLHINYYEQRAKGGTGLIIVENASVDSPQGSNGTTQLRID
LDNYIPRLFKLCESVHKHGACIAIQLNHAGASAMSSRIGMQPVSASDVPSKAGGEIPRPLEKDEIMHIVKKYGEAAKRAQ
ICGFDAVEIHAGHSYLISQFLSPITNKRTDEFGGSAENRARFAKLVIEEVRKQVGPFFPIFVRISADELMEGGNTLEDTL
EYLKYFEKEVDVFDVSCGLNGSIQYQIDANYLPDGWRSFMAKAVKEKYNKPCITVGNIRDPQVAEDILAGGDADFIGMGR
GLIADPEWVNKVEFGNVCDIRKCISCNIGCAGHRIGLNQPIRCTVNPAVNSGEDYMKTKVNKPCNVVVIGGGTAGLEAAC
TAAEVGCTTFLIEKKAELGGLASVISKIPDKKRLADFPNYMIHRASKLHNLFVFKNTSATVDMVKALNPDIIVNATGSVP
TLPPITGLHDLVDKDGTNVATVLKMIERINEYPEDMKGQKIAIIGGGAVGLDVMEFFTERGAEVTMVEMLPMIGNGLDPV
TKCDTNAKMAKYGVKQMTNTALQEVKNDRFIVKNPEGEIEEIPFDYGFICLGMRANTPVLSEIDEAFSNTNVEIVNIGDS
KRARRIIEGTEEGRNILNVLARHDYL

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=543, Percent_Identity=31.1233885819521, Blast_Score=239, Evalue=5e-64,
Organism=Escherichia coli, GI1787939, Length=347, Percent_Identity=28.5302593659942, Blast_Score=113, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17559804, Length=310, Percent_Identity=29.3548387096774, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI72001454, Length=317, Percent_Identity=29.9684542586751, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17564188, Length=315, Percent_Identity=27.9365079365079, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17559802, Length=306, Percent_Identity=29.0849673202614, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17540738, Length=310, Percent_Identity=27.0967741935484, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17565138, Length=305, Percent_Identity=27.8688524590164, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17566914, Length=350, Percent_Identity=25.1428571428571, Blast_Score=89, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6321973, Length=353, Percent_Identity=28.6118980169972, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6325086, Length=227, Percent_Identity=32.15859030837, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 73116; Mature: 73116

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKYPHIFEPITVRRMTVKNRIVMTPMGTNYGEQNGEMSFLHINYYEQRAKGGTGLIIV
CCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCEEEEEEEHHHHHCCCCCEEEEE
ENASVDSPQGSNGTTQLRIDLDNYIPRLFKLCESVHKHGACIAIQLNHAGASAMSSRIGM
ECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCC
QPVSASDVPSKAGGEIPRPLEKDEIMHIVKKYGEAAKRAQICGFDAVEIHAGHSYLISQF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHH
LSPITNKRTDEFGGSAENRARFAKLVIEEVRKQVGPFFPIFVRISADELMEGGNTLEDTL
HHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHCCCCCHHHHH
EYLKYFEKEVDVFDVSCGLNGSIQYQIDANYLPDGWRSFMAKAVKEKYNKPCITVGNIRD
HHHHHHHHCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCC
PQVAEDILAGGDADFIGMGRGLIADPEWVNKVEFGNVCDIRKCISCNIGCAGHRIGLNQP
HHHHHHHHCCCCCCEEECCCCCCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC
IRCTVNPAVNSGEDYMKTKVNKPCNVVVIGGGTAGLEAACTAAEVGCTTFLIEKKAELGG
EEEEECCCCCCCHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEECHHHHHH
LASVISKIPDKKRLADFPNYMIHRASKLHNLFVFKNTSATVDMVKALNPDIIVNATGSVP
HHHHHHHCCCHHHHHHCHHHHHHHHHHHCEEEEEECCCCHHHHHHHCCCCEEEECCCCCC
TLPPITGLHDLVDKDGTNVATVLKMIERINEYPEDMKGQKIAIIGGGAVGLDVMEFFTER
CCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCCCEEEEEECCHHHHHHHHHHHHC
GAEVTMVEMLPMIGNGLDPVTKCDTNAKMAKYGVKQMTNTALQEVKNDRFIVKNPEGEIE
CCCEEHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHH
EIPFDYGFICLGMRANTPVLSEIDEAFSNTNVEIVNIGDSKRARRIIEGTEEGRNILNVL
HCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHH
ARHDYL
HHCCCC
>Mature Secondary Structure
MKNKYPHIFEPITVRRMTVKNRIVMTPMGTNYGEQNGEMSFLHINYYEQRAKGGTGLIIV
CCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCEEEEEEEHHHHHCCCCCEEEEE
ENASVDSPQGSNGTTQLRIDLDNYIPRLFKLCESVHKHGACIAIQLNHAGASAMSSRIGM
ECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCC
QPVSASDVPSKAGGEIPRPLEKDEIMHIVKKYGEAAKRAQICGFDAVEIHAGHSYLISQF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHH
LSPITNKRTDEFGGSAENRARFAKLVIEEVRKQVGPFFPIFVRISADELMEGGNTLEDTL
HHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHCCCCCHHHHH
EYLKYFEKEVDVFDVSCGLNGSIQYQIDANYLPDGWRSFMAKAVKEKYNKPCITVGNIRD
HHHHHHHHCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCC
PQVAEDILAGGDADFIGMGRGLIADPEWVNKVEFGNVCDIRKCISCNIGCAGHRIGLNQP
HHHHHHHHCCCCCCEEECCCCCCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC
IRCTVNPAVNSGEDYMKTKVNKPCNVVVIGGGTAGLEAACTAAEVGCTTFLIEKKAELGG
EEEEECCCCCCCHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEECHHHHHH
LASVISKIPDKKRLADFPNYMIHRASKLHNLFVFKNTSATVDMVKALNPDIIVNATGSVP
HHHHHHHCCCHHHHHHCHHHHHHHHHHHCEEEEEECCCCHHHHHHHCCCCEEEECCCCCC
TLPPITGLHDLVDKDGTNVATVLKMIERINEYPEDMKGQKIAIIGGGAVGLDVMEFFTER
CCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCCCEEEEEECCHHHHHHHHHHHHC
GAEVTMVEMLPMIGNGLDPVTKCDTNAKMAKYGVKQMTNTALQEVKNDRFIVKNPEGEIE
CCCEEHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHH
EIPFDYGFICLGMRANTPVLSEIDEAFSNTNVEIVNIGDSKRARRIIEGTEEGRNILNVL
HCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHH
ARHDYL
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]