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The map label for this gene is yclM [H]

Identifier: 238925143

GI number: 238925143

Start: 2674420

End: 2675739

Strand: Reverse

Name: yclM [H]

Synonym: EUBREC_2796

Alternate gene names: 238925143

Gene position: 2675739-2674420 (Counterclockwise)

Preceding gene: 238925144

Following gene: 238925141

Centisome position: 77.56

GC content: 45.0

Gene sequence:

>1320_bases
ATGGTAAAGGTAGTAAAGTTTGGCGGCAGCTCGCTCGCCAGTGCAGAGCAGTTCACAAAGGTTGGCGACATCATAAGAAG
CGATGAGTCAAGAAAATATGTTGTGCCGAGTGCACCGGGAAAGAGAAATTCCAAAGACACAAAGGTAACAGATATGCTTT
ATGCATGCTATGATCTGGCTGAGAATGATCAGGATTTCAAGGTTATGCTCAGAAAAATCAAGGACAGATATGATTCAATC
ATAAATGGCCTTCATTTAAAGCTGGCACTTGATGAGGAGTTCAAGATTATCGCTGAGAATTTCAAGGCAAAGGCAGGTGC
AGATTATGCAGCCTCTCGTGGTGAGTACTTAAACGGAATCATCATGGCAAACTATCTCGGATATGAGTTTATAGATTCGG
CAACAGTCATCTTTTTTGATGAGAATGGCAATTTTGACGCGGAAAAGACAGACAAGGTGCTCTCAAAGAAGCTTGAGCAG
ACAGAAAAGGCTGTTATTCCGGGCTTTTACGGAGCAGGACCTGATGGAAAGGTAGTCACCTTCTCAAGAGGTGGTTCGGA
TATCACTGGTTCCATCGTGTCAAAGGCATGTCATGCCAGCATGTACGAGAACTGGACAGATGTTTCAGGCTGTCTCGTAG
CTGATCCTAGAATTATCGACAATCCACAGCCAATCAAGGTTGTTACTTATAGAGAGCTCAGAGAGCTTTCATATATGGGA
GCATCAGTGCTCCACGAGGATGCTGTTTTCCCTGTAAGAAAGGCAGGCATCCCAATCAATATCAGAAATACAAACGACCC
GGAAGCACCTGGTACTCTCATCGTTGAGAGCACATGCCAAAAGCCTGATTATACCATCACAGGTATCGCCGGAAAGAAGG
GCTTTGTTGCGGTAAATATCGACAAGGACAAGATGAACTCAGAGGTGGGCTTCTGTAGAAAGGCTCTCCAGGCGTTTGAG
GAGAACGGTATCTCTATAGAGCATATGCCATCAGGTATCGATACTATGACAGTATTCGTACATCAGGCAGAGTTTGAGGG
CAAGGAGCAGCAGGTTATCAGCTCAATCAGAAGACTTGCTCATCCTGATATCATTGACCTCGAGTCAGACCTCGCGCTTA
TCGCTGTTGTTGGACGTGGCATGAAGTCAAACCGTGGTACAGCCGGCAGGATTTTCTCTGCACTTGCACATGCCAATATC
AATGTGCGCATGATTGATCAGGGTTCATCAGAGCTCAATATCATCATCGGTGTAAGCAATGATGACTTTGACAAGGCAAT
AAAAGCCATCTACGATATTTTTGTTGTCACACAGCTGTAA

Upstream 100 bases:

>100_bases
TTGATGTTGATTATATTGAGTATTGACGAATGATTTATCAAAGCGGTATAATATTGGCTAATACAGTTATTTATATAGGA
AGCAGAGGAAAAACAGACAA

Downstream 100 bases:

>100_bases
TAAGCTACAGAGAGATTATCCGTTGCAACCGGAATTGAATAGGATAATTTGAAAATTAAATGTAGAATTTATAAAATATA
CAGGGCTTGGCGGAATATAT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MVKVVKFGGSSLASAEQFTKVGDIIRSDESRKYVVPSAPGKRNSKDTKVTDMLYACYDLAENDQDFKVMLRKIKDRYDSI
INGLHLKLALDEEFKIIAENFKAKAGADYAASRGEYLNGIIMANYLGYEFIDSATVIFFDENGNFDAEKTDKVLSKKLEQ
TEKAVIPGFYGAGPDGKVVTFSRGGSDITGSIVSKACHASMYENWTDVSGCLVADPRIIDNPQPIKVVTYRELRELSYMG
ASVLHEDAVFPVRKAGIPINIRNTNDPEAPGTLIVESTCQKPDYTITGIAGKKGFVAVNIDKDKMNSEVGFCRKALQAFE
ENGISIEHMPSGIDTMTVFVHQAEFEGKEQQVISSIRRLAHPDIIDLESDLALIAVVGRGMKSNRGTAGRIFSALAHANI
NVRMIDQGSSELNIIIGVSNDDFDKAIKAIYDIFVVTQL

Sequences:

>Translated_439_residues
MVKVVKFGGSSLASAEQFTKVGDIIRSDESRKYVVPSAPGKRNSKDTKVTDMLYACYDLAENDQDFKVMLRKIKDRYDSI
INGLHLKLALDEEFKIIAENFKAKAGADYAASRGEYLNGIIMANYLGYEFIDSATVIFFDENGNFDAEKTDKVLSKKLEQ
TEKAVIPGFYGAGPDGKVVTFSRGGSDITGSIVSKACHASMYENWTDVSGCLVADPRIIDNPQPIKVVTYRELRELSYMG
ASVLHEDAVFPVRKAGIPINIRNTNDPEAPGTLIVESTCQKPDYTITGIAGKKGFVAVNIDKDKMNSEVGFCRKALQAFE
ENGISIEHMPSGIDTMTVFVHQAEFEGKEQQVISSIRRLAHPDIIDLESDLALIAVVGRGMKSNRGTAGRIFSALAHANI
NVRMIDQGSSELNIIIGVSNDDFDKAIKAIYDIFVVTQL
>Mature_439_residues
MVKVVKFGGSSLASAEQFTKVGDIIRSDESRKYVVPSAPGKRNSKDTKVTDMLYACYDLAENDQDFKVMLRKIKDRYDSI
INGLHLKLALDEEFKIIAENFKAKAGADYAASRGEYLNGIIMANYLGYEFIDSATVIFFDENGNFDAEKTDKVLSKKLEQ
TEKAVIPGFYGAGPDGKVVTFSRGGSDITGSIVSKACHASMYENWTDVSGCLVADPRIIDNPQPIKVVTYRELRELSYMG
ASVLHEDAVFPVRKAGIPINIRNTNDPEAPGTLIVESTCQKPDYTITGIAGKKGFVAVNIDKDKMNSEVGFCRKALQAFE
ENGISIEHMPSGIDTMTVFVHQAEFEGKEQQVISSIRRLAHPDIIDLESDLALIAVVGRGMKSNRGTAGRIFSALAHANI
NVRMIDQGSSELNIIIGVSNDDFDKAIKAIYDIFVVTQL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=472, Percent_Identity=28.8135593220339, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1790455, Length=456, Percent_Identity=24.5614035087719, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1790376, Length=296, Percent_Identity=24.6621621621622, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320893, Length=497, Percent_Identity=25.5533199195171, Blast_Score=126, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 48213; Mature: 48213

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKVVKFGGSSLASAEQFTKVGDIIRSDESRKYVVPSAPGKRNSKDTKVTDMLYACYDLA
CEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHH
ENDQDFKVMLRKIKDRYDSIINGLHLKLALDEEFKIIAENFKAKAGADYAASRGEYLNGI
CCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHCCCCCCCCHHHCCCCHHCCE
IMANYLGYEFIDSATVIFFDENGNFDAEKTDKVLSKKLEQTEKAVIPGFYGAGPDGKVVT
EEEHHCCHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
FSRGGSDITGSIVSKACHASMYENWTDVSGCLVADPRIIDNPQPIKVVTYRELRELSYMG
EECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCEECCCCCCEEEEEHHHHHHHHHHH
ASVLHEDAVFPVRKAGIPINIRNTNDPEAPGTLIVESTCQKPDYTITGIAGKKGFVAVNI
HHHHHHCCCCCHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEEE
DKDKMNSEVGFCRKALQAFEENGISIEHMPSGIDTMTVFVHQAEFEGKEQQVISSIRRLA
CHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHC
HPDIIDLESDLALIAVVGRGMKSNRGTAGRIFSALAHANINVRMIDQGSSELNIIIGVSN
CCCEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCC
DDFDKAIKAIYDIFVVTQL
CCHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MVKVVKFGGSSLASAEQFTKVGDIIRSDESRKYVVPSAPGKRNSKDTKVTDMLYACYDLA
CEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHH
ENDQDFKVMLRKIKDRYDSIINGLHLKLALDEEFKIIAENFKAKAGADYAASRGEYLNGI
CCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHCCCCCCCCHHHCCCCHHCCE
IMANYLGYEFIDSATVIFFDENGNFDAEKTDKVLSKKLEQTEKAVIPGFYGAGPDGKVVT
EEEHHCCHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
FSRGGSDITGSIVSKACHASMYENWTDVSGCLVADPRIIDNPQPIKVVTYRELRELSYMG
EECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCEECCCCCCEEEEEHHHHHHHHHHH
ASVLHEDAVFPVRKAGIPINIRNTNDPEAPGTLIVESTCQKPDYTITGIAGKKGFVAVNI
HHHHHHCCCCCHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEEE
DKDKMNSEVGFCRKALQAFEENGISIEHMPSGIDTMTVFVHQAEFEGKEQQVISSIRRLA
CHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHC
HPDIIDLESDLALIAVVGRGMKSNRGTAGRIFSALAHANINVRMIDQGSSELNIIIGVSN
CCCEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCC
DDFDKAIKAIYDIFVVTQL
CCHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]