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The map label for this gene is lacA [C]

Identifier: 238925003

GI number: 238925003

Start: 2531199

End: 2531702

Strand: Reverse

Name: lacA [C]

Synonym: EUBREC_2654

Alternate gene names: 238925003

Gene position: 2531702-2531199 (Counterclockwise)

Preceding gene: 238925004

Following gene: 238925002

Centisome position: 73.39

GC content: 35.12

Gene sequence:

>504_bases
ATGGGAATAATATCTAGAGGAAAAGCTTTATGGGCAAAGATTTATTATCAAAAAAGAATAAAAATGAAAGCTGCTGCTTG
TGGTGAAAAATTATATGTAGGCGGAAAATCGTTTGTTAATTCAAATACGTATCTTGGATATGGGGTGTGTTTTAATGGAA
TGTCGATTTTGGGACAGGGAAAGGTTGAGATTGGAAATTATTTTCATTCAGGGACGGAGTGCCAGATTATAACTTCATTT
CATAATTATGAAGGAGAGCAGATACCTTACGACAACTCTTATATAACTAAGAATGTTAAAATTGGTGATTGTGTATGGCT
GGGAAATAATGTAATAATCTTGGGAGGCTGTGTAATAGGAGAGGGAGCCATTATACAGGCTGGATCAGTTGTATGCAAGG
ATATACCTGCATATTCAATAGCAGGAGGGCATCCGGCCATTCCGTTTAAAACTAGAGACAAAGAACATTATTTAAAAATG
AAAAAGGAAGAACGTTTTCATTAA

Upstream 100 bases:

>100_bases
GGGGACTATATGAGTTTACCACCTGAATCAGAAAGAATTGTTCATTCGAATTATAAAATAGATTTTGGATTATATGATGG
ATTGTTGGAGGATATGCTAT

Downstream 100 bases:

>100_bases
CTGGTAAGATTGTATATGAATAAATTATTGAATAAATATAATAAAATATCAGAACCGGTAAAAGCATCAATATGGTATAC
AATATGTAATGTGATAAATA

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MGIISRGKALWAKIYYQKRIKMKAAACGEKLYVGGKSFVNSNTYLGYGVCFNGMSILGQGKVEIGNYFHSGTECQIITSF
HNYEGEQIPYDNSYITKNVKIGDCVWLGNNVIILGGCVIGEGAIIQAGSVVCKDIPAYSIAGGHPAIPFKTRDKEHYLKM
KKEERFH

Sequences:

>Translated_167_residues
MGIISRGKALWAKIYYQKRIKMKAAACGEKLYVGGKSFVNSNTYLGYGVCFNGMSILGQGKVEIGNYFHSGTECQIITSF
HNYEGEQIPYDNSYITKNVKIGDCVWLGNNVIILGGCVIGEGAIIQAGSVVCKDIPAYSIAGGHPAIPFKTRDKEHYLKM
KKEERFH
>Mature_166_residues
GIISRGKALWAKIYYQKRIKMKAAACGEKLYVGGKSFVNSNTYLGYGVCFNGMSILGQGKVEIGNYFHSGTECQIITSFH
NYEGEQIPYDNSYITKNVKIGDCVWLGNNVIILGGCVIGEGAIIQAGSVVCKDIPAYSIAGGHPAIPFKTRDKEHYLKMK
KEERFH

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 18441; Mature: 18309

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIISRGKALWAKIYYQKRIKMKAAACGEKLYVGGKSFVNSNTYLGYGVCFNGMSILGQG
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHCCCCCEEEEEEEECCEEEEECC
KVEIGNYFHSGTECQIITSFHNYEGEQIPYDNSYITKNVKIGDCVWLGNNVIILGGCVIG
CEEECCEECCCCEEEEEEEEECCCCCCCCCCCCEEEECCEECEEEEECCCEEEEEEEEEC
EGAIIQAGSVVCKDIPAYSIAGGHPAIPFKTRDKEHYLKMKKEERFH
CCEEEECCCEEECCCCCEEECCCCCCCCCCCCCHHHHHHCCHHCCCC
>Mature Secondary Structure 
GIISRGKALWAKIYYQKRIKMKAAACGEKLYVGGKSFVNSNTYLGYGVCFNGMSILGQG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHCCCCCEEEEEEEECCEEEEECC
KVEIGNYFHSGTECQIITSFHNYEGEQIPYDNSYITKNVKIGDCVWLGNNVIILGGCVIG
CEEECCEECCCCEEEEEEEEECCCCCCCCCCCCEEEECCEECEEEEECCCEEEEEEEEEC
EGAIIQAGSVVCKDIPAYSIAGGHPAIPFKTRDKEHYLKMKKEERFH
CCEEEECCCEEECCCCCEEECCCCCCCCCCCCCHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]