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The map label for this gene is ytzG [H]

Identifier: 238924043

GI number: 238924043

Start: 1563504

End: 1564256

Strand: Direct

Name: ytzG [H]

Synonym: EUBREC_1681

Alternate gene names: 238924043

Gene position: 1563504-1564256 (Clockwise)

Preceding gene: 238924041

Following gene: 238924044

Centisome position: 45.32

GC content: 43.29

Gene sequence:

>753_bases
GTGACAGTATATGTGGAGAGAAATATCAATATGATTCGATTAGATAAATTTTTATCAGAGATGGGGCTTGGCACACGCTC
TGAGGTAAAAAAGCTTATAAAGACCGGACAGATAGCCATAAATGGCACTGTGGCCGCAAAGCCTGAGACAAAGGTAGATA
CTGATGCCGACGAGGTATCAGTTAACGGCAGAATAGTTAAGTATCAAAGGTATGAGTATTACCTGTTTAATAAGCCCTCA
GGCTGTGTTTCTGCGACCTGTGATAATGTGCATGATACCGTCATGGACTATATCACGGATGCTGTGCATGATGATCTGTT
TCCTGTCGGAAGGCTTGATATAGACACGGAGGGGCTTTTACTTATCACAAATGATGGAGCGCTTTCGCACGAGCTCCTAA
GTCCTTCAAAGCACGTGGCAAAGACTTACTACGCACGTATTGATGGTGAGGTGACTTTGGATGATATCATCAGGTTTAAA
GAGGGCATTGATATAGGTGAGGAAAAACCTACAAAGCCTGCGGAGCTTGTTATTAAAAAAAGCGGCAGCGTATCCGAGAT
TGAGCTTACGATAACAGAAGGAAAGTTCCATCAGGTCAAGCGGATGTTTGAGGCGCTTGGAAAGAGGGTAATCTATTTAA
AGAGAATATCCATGGGACCGCTTAAGCTGCCTGATGACCTTAAAACAGGGACTTACAGAGCTTTGACACAGGCTGAAACG
GATATGCTTATGCAGATAAAATCCGGCAAATAA

Upstream 100 bases:

>100_bases
TAGCGCACGTACTTACCTGTGCCGGAAACAATATGTAATAAATACAAAATAGCAGTATAAAAGCGATAAACAGTAATTTT
TTAAGTTTGTTTTTACTCAT

Downstream 100 bases:

>100_bases
AATAATTTTTAATTATGAGGTATGATATGAGTAATGGTTTTTTACCAATTTCAAAACAGGATATGATAGATGAGGGCATT
GAGCAGCTTGATTTTGTGTA

Product: 16S pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTVYVERNINMIRLDKFLSEMGLGTRSEVKKLIKTGQIAINGTVAAKPETKVDTDADEVSVNGRIVKYQRYEYYLFNKPS
GCVSATCDNVHDTVMDYITDAVHDDLFPVGRLDIDTEGLLLITNDGALSHELLSPSKHVAKTYYARIDGEVTLDDIIRFK
EGIDIGEEKPTKPAELVIKKSGSVSEIELTITEGKFHQVKRMFEALGKRVIYLKRISMGPLKLPDDLKTGTYRALTQAET
DMLMQIKSGK

Sequences:

>Translated_250_residues
MTVYVERNINMIRLDKFLSEMGLGTRSEVKKLIKTGQIAINGTVAAKPETKVDTDADEVSVNGRIVKYQRYEYYLFNKPS
GCVSATCDNVHDTVMDYITDAVHDDLFPVGRLDIDTEGLLLITNDGALSHELLSPSKHVAKTYYARIDGEVTLDDIIRFK
EGIDIGEEKPTKPAELVIKKSGSVSEIELTITEGKFHQVKRMFEALGKRVIYLKRISMGPLKLPDDLKTGTYRALTQAET
DMLMQIKSGK
>Mature_249_residues
TVYVERNINMIRLDKFLSEMGLGTRSEVKKLIKTGQIAINGTVAAKPETKVDTDADEVSVNGRIVKYQRYEYYLFNKPSG
CVSATCDNVHDTVMDYITDAVHDDLFPVGRLDIDTEGLLLITNDGALSHELLSPSKHVAKTYYARIDGEVTLDDIIRFKE
GIDIGEEKPTKPAELVIKKSGSVSEIELTITEGKFHQVKRMFEALGKRVIYLKRISMGPLKLPDDLKTGTYRALTQAETD
MLMQIKSGK

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=36.9565217391304, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1787524, Length=245, Percent_Identity=31.0204081632653, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790453, Length=241, Percent_Identity=29.8755186721992, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081838, Length=179, Percent_Identity=33.5195530726257, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 27994; Mature: 27862

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVYVERNINMIRLDKFLSEMGLGTRSEVKKLIKTGQIAINGTVAAKPETKVDTDADEVS
CEEEEECCCCEEHHHHHHHHHCCCCHHHHHHHHHCCCEEEECEEECCCCCCCCCCCHHEE
VNGRIVKYQRYEYYLFNKPSGCVSATCDNVHDTVMDYITDAVHDDLFPVGRLDIDTEGLL
ECCEEEEEEEEEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEE
LITNDGALSHELLSPSKHVAKTYYARIDGEVTLDDIIRFKEGIDIGEEKPTKPAELVIKK
EEECCCCCHHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCCCCHHEEEEC
SGSVSEIELTITEGKFHQVKRMFEALGKRVIYLKRISMGPLKLPDDLKTGTYRALTQAET
CCCEEEEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
DMLMQIKSGK
HEEEEEECCC
>Mature Secondary Structure 
TVYVERNINMIRLDKFLSEMGLGTRSEVKKLIKTGQIAINGTVAAKPETKVDTDADEVS
EEEEECCCCEEHHHHHHHHHCCCCHHHHHHHHHCCCEEEECEEECCCCCCCCCCCHHEE
VNGRIVKYQRYEYYLFNKPSGCVSATCDNVHDTVMDYITDAVHDDLFPVGRLDIDTEGLL
ECCEEEEEEEEEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEE
LITNDGALSHELLSPSKHVAKTYYARIDGEVTLDDIIRFKEGIDIGEEKPTKPAELVIKK
EEECCCCCHHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCCCCHHEEEEC
SGSVSEIELTITEGKFHQVKRMFEALGKRVIYLKRISMGPLKLPDDLKTGTYRALTQAET
CCCEEEEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
DMLMQIKSGK
HEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]