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The map label for this gene is rimO [H]

Identifier: 238924037

GI number: 238924037

Start: 1559001

End: 1560326

Strand: Direct

Name: rimO [H]

Synonym: EUBREC_1675

Alternate gene names: 238924037

Gene position: 1559001-1560326 (Clockwise)

Preceding gene: 238924036

Following gene: 238924038

Centisome position: 45.19

GC content: 43.36

Gene sequence:

>1326_bases
ATGAAATTATTATTTATTTCGCTTGGATGCGATAAAAACCTTGTGGATTCAGAGTATATGATAGGCATGCTTGCAAATGA
CGGCATTGAGATGACCGATGATGAGACGCAGGCTGATATCATTATCGTAAACAGCTGCTGCTTCATAGGTGATGCAAAGG
AGGAGAGTATCAATACAATTCTCGAGATGGCTCAGTATAAGGAGACAGGCAAATGCAAATCACTTATTGTGACAGGCTGT
CTGGCACAAAGATACAAGGATGAGATTTTTAAGGAAATCCCAGAGGTTGATGCCATACTTGGCACCAATTCATATGATAC
TATAGTTGAAGCTGTCCATGAGACACTTGAAAAACACAGATACAGCAATCTGCACACCTTAGAGGGGCTTCCCAGCATAA
AGACAAAGCGTATTGTCACCACAGGAGGGCATTTCGCTTACCTTAAGATAGCAGAGGGTTGCAATAAAAACTGTACCTAC
TGTGTCATTCCATCTGTCAGAGGTCGTTACAGAAGTGTGCCGATGGAGGATTTGATTGAGCAGGCGAAGAGCCTTGTGGA
GCAGGGGGCAAAGGAGCTTATACTTGTTGCCCAGGAGACCACACTTTACGGAGTGGATTTGTATGGTGAGAAGTCACTTC
ATAAGCTTTTGGATGAGCTCAACAAGATAAGCGGGCTGTTCTGGATAAGGATTATGTACTGCTATCCCGAGGAGATATAC
GATGAGCTTATAGAGTCCATGATAAAGGATGAGAAGGTATGCCATTATCTGGATATGCCAATCCAGCACTGCAATGACGA
TATTTTAAGGCGCATGGGCAGAAAAACAACAAAGGCTGAGCTTATGGAGCGTATCAGAATGCTGCGTGAGAGAATACCTG
ATATCACACTAAGGACCACACTTATATGTGGCTTCCCGGGTGAGACACAGGATAATCACGAGGAGCTTATGCAGTTTGTA
AATGATATGGAGTTTGACCGTCTGGGAGCCTTTACGTATTCCCCGGAGGAGGGCACAAAGGCAGCTGCAATGCCTGACCA
GATAGATGAGGATATAAAGGCTGACTGGCAGGCTGATGTCATGGAGCTTGAGGAAGAGGTCATTTTTGATAAAAATGAGA
CACTAAAGGGTAAAGAATTATATGTTTTCATAGAGGGCAAGGTGGCTGATGAAAATGCTTATATCGGCCGTACATACAGA
GATGCACCGAATGTTGACGGATATATATTCATCAACACTGATGAGACACTTATGACCGGTGATATATGCAAGGTTATGGT
GACGGGTGCGTATGAATATGATTTGATAGGAGAACTTGTAAAATGA

Upstream 100 bases:

>100_bases
ATGATGAGGATTTAAACGAAGGCGAAGAGGCTTAATCTAGGAGGGGAAAGGCTGTTTTGTCTTTCCCTTTAATTGTAAAT
ACTTACATTTGAGAGGTACT

Downstream 100 bases:

>100_bases
ATTTACCAAACAAACTTACAATCTTAAGAGTGATACTTATTCCGTTTTTTGTATTTTTCCTTATTTCACCGTTTTTTGAC
GGATACGGAAATTATATAGC

Product: MiaB-like tRNA modifying enzyme YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKLLFISLGCDKNLVDSEYMIGMLANDGIEMTDDETQADIIIVNSCCFIGDAKEESINTILEMAQYKETGKCKSLIVTGC
LAQRYKDEIFKEIPEVDAILGTNSYDTIVEAVHETLEKHRYSNLHTLEGLPSIKTKRIVTTGGHFAYLKIAEGCNKNCTY
CVIPSVRGRYRSVPMEDLIEQAKSLVEQGAKELILVAQETTLYGVDLYGEKSLHKLLDELNKISGLFWIRIMYCYPEEIY
DELIESMIKDEKVCHYLDMPIQHCNDDILRRMGRKTTKAELMERIRMLRERIPDITLRTTLICGFPGETQDNHEELMQFV
NDMEFDRLGAFTYSPEEGTKAAAMPDQIDEDIKADWQADVMELEEEVIFDKNETLKGKELYVFIEGKVADENAYIGRTYR
DAPNVDGYIFINTDETLMTGDICKVMVTGAYEYDLIGELVK

Sequences:

>Translated_441_residues
MKLLFISLGCDKNLVDSEYMIGMLANDGIEMTDDETQADIIIVNSCCFIGDAKEESINTILEMAQYKETGKCKSLIVTGC
LAQRYKDEIFKEIPEVDAILGTNSYDTIVEAVHETLEKHRYSNLHTLEGLPSIKTKRIVTTGGHFAYLKIAEGCNKNCTY
CVIPSVRGRYRSVPMEDLIEQAKSLVEQGAKELILVAQETTLYGVDLYGEKSLHKLLDELNKISGLFWIRIMYCYPEEIY
DELIESMIKDEKVCHYLDMPIQHCNDDILRRMGRKTTKAELMERIRMLRERIPDITLRTTLICGFPGETQDNHEELMQFV
NDMEFDRLGAFTYSPEEGTKAAAMPDQIDEDIKADWQADVMELEEEVIFDKNETLKGKELYVFIEGKVADENAYIGRTYR
DAPNVDGYIFINTDETLMTGDICKVMVTGAYEYDLIGELVK
>Mature_441_residues
MKLLFISLGCDKNLVDSEYMIGMLANDGIEMTDDETQADIIIVNSCCFIGDAKEESINTILEMAQYKETGKCKSLIVTGC
LAQRYKDEIFKEIPEVDAILGTNSYDTIVEAVHETLEKHRYSNLHTLEGLPSIKTKRIVTTGGHFAYLKIAEGCNKNCTY
CVIPSVRGRYRSVPMEDLIEQAKSLVEQGAKELILVAQETTLYGVDLYGEKSLHKLLDELNKISGLFWIRIMYCYPEEIY
DELIESMIKDEKVCHYLDMPIQHCNDDILRRMGRKTTKAELMERIRMLRERIPDITLRTTLICGFPGETQDNHEELMQFV
NDMEFDRLGAFTYSPEEGTKAAAMPDQIDEDIKADWQADVMELEEEVIFDKNETLKGKELYVFIEGKVADENAYIGRTYR
DAPNVDGYIFINTDETLMTGDICKVMVTGAYEYDLIGELVK

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=374, Percent_Identity=29.144385026738, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI28872784, Length=420, Percent_Identity=25, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI28872782, Length=420, Percent_Identity=25, Blast_Score=155, Evalue=8e-38,
Organism=Escherichia coli, GI1787057, Length=449, Percent_Identity=37.6391982182628, Blast_Score=313, Evalue=9e-87,
Organism=Escherichia coli, GI1786882, Length=451, Percent_Identity=28.3813747228381, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=469, Percent_Identity=25.3731343283582, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71996771, Length=277, Percent_Identity=30.3249097472924, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21356207, Length=470, Percent_Identity=25.3191489361702, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI19922432, Length=463, Percent_Identity=27.2138228941685, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50370; Mature: 50370

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLFISLGCDKNLVDSEYMIGMLANDGIEMTDDETQADIIIVNSCCFIGDAKEESINTI
CEEEEEEECCCCCCCCCHHEEEEEECCCCCCCCCCCCCCEEEEECEEEECCCCHHHHHHH
LEMAQYKETGKCKSLIVTGCLAQRYKDEIFKEIPEVDAILGTNSYDTIVEAVHETLEKHR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHH
YSNLHTLEGLPSIKTKRIVTTGGHFAYLKIAEGCNKNCTYCVIPSVRGRYRSVPMEDLIE
CCCCHHHCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHH
QAKSLVEQGAKELILVAQETTLYGVDLYGEKSLHKLLDELNKISGLFWIRIMYCYPEEIY
HHHHHHHCCCHHEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
DELIESMIKDEKVCHYLDMPIQHCNDDILRRMGRKTTKAELMERIRMLRERIPDITLRTT
HHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEE
LICGFPGETQDNHEELMQFVNDMEFDRLGAFTYSPEEGTKAAAMPDQIDEDIKADWQADV
EEECCCCCCCCCHHHHHHHHHCCCHHHHCCEEECCCCCCCCCCCCHHHHHHHHCCHHHHH
MELEEEVIFDKNETLKGKELYVFIEGKVADENAYIGRTYRDAPNVDGYIFINTDETLMTG
HHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCEECCEECCCCCCCEEEEEECCCEEECC
DICKVMVTGAYEYDLIGELVK
HHHEEHEECCCHHHHHHHHCC
>Mature Secondary Structure
MKLLFISLGCDKNLVDSEYMIGMLANDGIEMTDDETQADIIIVNSCCFIGDAKEESINTI
CEEEEEEECCCCCCCCCHHEEEEEECCCCCCCCCCCCCCEEEEECEEEECCCCHHHHHHH
LEMAQYKETGKCKSLIVTGCLAQRYKDEIFKEIPEVDAILGTNSYDTIVEAVHETLEKHR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHH
YSNLHTLEGLPSIKTKRIVTTGGHFAYLKIAEGCNKNCTYCVIPSVRGRYRSVPMEDLIE
CCCCHHHCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHH
QAKSLVEQGAKELILVAQETTLYGVDLYGEKSLHKLLDELNKISGLFWIRIMYCYPEEIY
HHHHHHHCCCHHEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
DELIESMIKDEKVCHYLDMPIQHCNDDILRRMGRKTTKAELMERIRMLRERIPDITLRTT
HHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEE
LICGFPGETQDNHEELMQFVNDMEFDRLGAFTYSPEEGTKAAAMPDQIDEDIKADWQADV
EEECCCCCCCCCHHHHHHHHHCCCHHHHCCEEECCCCCCCCCCCCHHHHHHHHCCHHHHH
MELEEEVIFDKNETLKGKELYVFIEGKVADENAYIGRTYRDAPNVDGYIFINTDETLMTG
HHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCEECCEECCCCCCCEEEEEECCCEEECC
DICKVMVTGAYEYDLIGELVK
HHHEEHEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA