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The map label for this gene is proB [H]

Identifier: 238924010

GI number: 238924010

Start: 1535465

End: 1536379

Strand: Direct

Name: proB [H]

Synonym: EUBREC_1648

Alternate gene names: 238924010

Gene position: 1535465-1536379 (Clockwise)

Preceding gene: 238924008

Following gene: 238924011

Centisome position: 44.51

GC content: 44.26

Gene sequence:

>915_bases
ATGCGTAATGTAATTATGTATGCATTTAGACTTACAATAGGAAAGATGATAGAAATGAGTTTTTTGGACAATTATAAAAG
AGTGGTAATAAAGATAGGTTCATCGACGCTGACACATGCAGAGACGGGAAGCCTCAATTTTTCAAAGATGGAGCGTCTGG
TGCGCTCGATATGTGATTACAGAAACAGCGGTATGGATGTGTGCCTCGTAAGCTCCGGCGCTATCGCGGTTGGACGTGAT
GTGATAGGCATAAAGGAGCGCCCGAGCGACATTTCAATAAAGCAGGCATGCGCCGCAGTTGGACAGGGCAGGCTTATGAT
GACATATCAGAAGCTTTTTTCCGAGTATAATCAGAATTCCGGACAGGTGCTCATGACAAAGAATACCATAGTCAATCCTG
TTTCAAGACGCAATGTTATGAATACATTTGAGGAGCTTTTTGATCTGAACGTGGTGCCTATTGTCAATGAAAATGATACG
GTAAGCACATACGAGATGCAGTTTGGTGACAATGATACGCTGTCTGCTATTGTTACATCAATCACGAAGGCCGATCTGCT
CATACTGCTTTCTGATATAGACGGACTGTATTCCGACGATCCTCATGACAATCCGGATGCAAAGCTTATCAGAGAGGTTG
ATACGCTTGACAGGAAAATACTTGGCATGGCAAAGTCATCAACCGGCAGCGATGTCGGAACCGGCGGCATGGCGACAAAG
CTTACAGCGGCAAAGATTGCCACATATTCCGGTGCCGATATGATTATCGCAAACGGCGGCAATATGGGAATTTTATATGA
CATTATGAATGACAGATATACAGGAACACTTTTCCACAGGGCAAAGGATGCAGAGTTTGTGCTTGCGGATTATATACAAA
GAGAGATGGCACCTACATCTACAGGCGGCCAGTAA

Upstream 100 bases:

>100_bases
CCGAAAGCAACAGAAATATAATAACACAAAATAAAAAAATAGAGACAGTAGTAAAAATGATTTTGTATTTTGCATGAATA
TATGGTAAAGTAGTAAATGT

Downstream 100 bases:

>100_bases
TCAGCTATAAATTTGAAATCAGGAGAGTGCTATGACACAGGAAAAAATACAGCGAATCAATGAGCTTTACAGAAAGTCAC
AGGCAGAGGGACTTTCTGAG

Product: glutamate 5-kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRNVIMYAFRLTIGKMIEMSFLDNYKRVVIKIGSSTLTHAETGSLNFSKMERLVRSICDYRNSGMDVCLVSSGAIAVGRD
VIGIKERPSDISIKQACAAVGQGRLMMTYQKLFSEYNQNSGQVLMTKNTIVNPVSRRNVMNTFEELFDLNVVPIVNENDT
VSTYEMQFGDNDTLSAIVTSITKADLLILLSDIDGLYSDDPHDNPDAKLIREVDTLDRKILGMAKSSTGSDVGTGGMATK
LTAAKIATYSGADMIIANGGNMGILYDIMNDRYTGTLFHRAKDAEFVLADYIQREMAPTSTGGQ

Sequences:

>Translated_304_residues
MRNVIMYAFRLTIGKMIEMSFLDNYKRVVIKIGSSTLTHAETGSLNFSKMERLVRSICDYRNSGMDVCLVSSGAIAVGRD
VIGIKERPSDISIKQACAAVGQGRLMMTYQKLFSEYNQNSGQVLMTKNTIVNPVSRRNVMNTFEELFDLNVVPIVNENDT
VSTYEMQFGDNDTLSAIVTSITKADLLILLSDIDGLYSDDPHDNPDAKLIREVDTLDRKILGMAKSSTGSDVGTGGMATK
LTAAKIATYSGADMIIANGGNMGILYDIMNDRYTGTLFHRAKDAEFVLADYIQREMAPTSTGGQ
>Mature_304_residues
MRNVIMYAFRLTIGKMIEMSFLDNYKRVVIKIGSSTLTHAETGSLNFSKMERLVRSICDYRNSGMDVCLVSSGAIAVGRD
VIGIKERPSDISIKQACAAVGQGRLMMTYQKLFSEYNQNSGQVLMTKNTIVNPVSRRNVMNTFEELFDLNVVPIVNENDT
VSTYEMQFGDNDTLSAIVTSITKADLLILLSDIDGLYSDDPHDNPDAKLIREVDTLDRKILGMAKSSTGSDVGTGGMATK
LTAAKIATYSGADMIIANGGNMGILYDIMNDRYTGTLFHRAKDAEFVLADYIQREMAPTSTGGQ

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=297, Percent_Identity=32.996632996633, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI21361368, Length=299, Percent_Identity=32.7759197324415, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1786437, Length=258, Percent_Identity=41.8604651162791, Blast_Score=200, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI17569969, Length=279, Percent_Identity=33.6917562724014, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17569967, Length=279, Percent_Identity=33.6917562724014, Blast_Score=141, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6321822, Length=274, Percent_Identity=42.3357664233577, Blast_Score=205, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6320506, Length=230, Percent_Identity=45.2173913043478, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI21357643, Length=299, Percent_Identity=32.4414715719064, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 33388; Mature: 33388

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNVIMYAFRLTIGKMIEMSFLDNYKRVVIKIGSSTLTHAETGSLNFSKMERLVRSICDY
CCCCEEHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
RNSGMDVCLVSSGAIAVGRDVIGIKERPSDISIKQACAAVGQGRLMMTYQKLFSEYNQNS
CCCCCCEEEEECCCEEECCCEECCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHCCCC
GQVLMTKNTIVNPVSRRNVMNTFEELFDLNVVPIVNENDTVSTYEMQFGDNDTLSAIVTS
CEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHH
ITKADLLILLSDIDGLYSDDPHDNPDAKLIREVDTLDRKILGMAKSSTGSDVGTGGMATK
HHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LTAAKIATYSGADMIIANGGNMGILYDIMNDRYTGTLFHRAKDAEFVLADYIQREMAPTS
HHHHHHEEECCCCEEEECCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCC
TGGQ
CCCC
>Mature Secondary Structure
MRNVIMYAFRLTIGKMIEMSFLDNYKRVVIKIGSSTLTHAETGSLNFSKMERLVRSICDY
CCCCEEHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
RNSGMDVCLVSSGAIAVGRDVIGIKERPSDISIKQACAAVGQGRLMMTYQKLFSEYNQNS
CCCCCCEEEEECCCEEECCCEECCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHCCCC
GQVLMTKNTIVNPVSRRNVMNTFEELFDLNVVPIVNENDTVSTYEMQFGDNDTLSAIVTS
CEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHH
ITKADLLILLSDIDGLYSDDPHDNPDAKLIREVDTLDRKILGMAKSSTGSDVGTGGMATK
HHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LTAAKIATYSGADMIIANGGNMGILYDIMNDRYTGTLFHRAKDAEFVLADYIQREMAPTS
HHHHHHEEECCCCEEEECCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCC
TGGQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA