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The map label for this gene is melB [H]

Identifier: 238923954

GI number: 238923954

Start: 1479347

End: 1480705

Strand: Direct

Name: melB [H]

Synonym: EUBREC_1592

Alternate gene names: 238923954

Gene position: 1479347-1480705 (Clockwise)

Preceding gene: 238923953

Following gene: 238923955

Centisome position: 42.88

GC content: 45.11

Gene sequence:

>1359_bases
ATGAAGCTTAAAACAGGACAAAAGTGTGCATTTGGATTTGGTGCATTCGGAAAAGATATTGTGTATATGCTTATTTCATC
GTATTTGCTCTACTACTACAATGTAGTGCTTGGGATGAGCAGTGTGTTTATCGGTACGGTGATGATGGCGGCCAGGATTT
TTGATGCATTCAATGACCCGGTGATGGGTATTATCGTAGCAAAGACACGTACTAGGTGGGGGAGGTTTAGGCCTTGGATT
TTTGCCGGCACGGTGCTCAATGCCGTGACCATCTATGCTTTGTATGCCACTCCTGAGTCTATGGGTGATTCAGCGCAGAG
AGTGTGGCTTACGGTGTTTTACTTTGCATGGGGCATCACATATACGCTTATGGATATTCCGTTCTGGTCTATGATTCCCG
CAATCACTGAGCCGGGCAGCGACAGGGAGCAGCTTACATCGCTTGCGAGGACATGCTCTGGCATCGGAGATGCTATCCCT
ACGGTGCTCACGATGACAGTGGTGCCTATTCTTGGTGCAGGAAGCTCTATGGCTGACTACAGGATTGGATTTAAGTGGTG
GGCACTTATTATAGCGGTAGTATTTGTGATTTCTGAGCTTGTATGCGTGGCATGTGTGCCTGAAAGACTGGTTGCCTGTG
ATGAGAAGCCGGGAAAGATATCTGATATGTTTAAAGCCTTATTTAAGAACGACCAGGCTATGACTGTTGTAGTATCAATC
ATTCTCGTGTATACATCACTCAATATCTGTGGCAACCTAGTGCTTTATTTCTTCAGGTTTGATGTGGGAAACGAGGGCGC
ATATTCCTTATTTGCTGCAGTTGCTTTTGCAGCACAGGTGCTTGTCATGATGGTTATACCGCTGCTTCGCAAGAGATTCA
GCAAAAGCCAGCTTTTTATATCAGGATTTATTATTCAGATAGCAGGCTTTGCGGTGATACTTATCATGGCATTTGCAAAG
CTCTACACGCCGCAGAGCTGGTACATACTCTGCCTGCCGGGTATTTTGATTTATCTGGGCTATGGTATGCTCAATGTTAT
CATGACTATATTTCTTTCAGATTCTGTTGATTATGGTGAGGTGATGAATGGCACGAGAGAGGAGTCGGTCATTTTCTCAA
TGCAGACATTTACGGTAAAGCTCGCATCCGGTGTAGCAGTATTTCTGGCTGGGCTGGTGGTTGACTGGATTCATCTGGAT
ACCGCGGCTGCGGTACAGACACAGGATACACTTGATGCTCTTCGCATGTGGATGACGATTCCGTCTGTTATCCTGCTCAT
CGTGGGCATATTTGTGTTTAAGAGGTTTTATAAGCTGGATGATACAAGGATGGAAGAAATTAAATCCAAGCTTAATTAA

Upstream 100 bases:

>100_bases
AGTAAATTATATTTGTAAGCACATTAACTTTAAATTGTATTTTACATAGCAATCCGCTATACTAGTATTAAGTATAGCGG
ATTATTTTGGAGGTATTTAT

Downstream 100 bases:

>100_bases
AAATTGCTATCTGATTGATAAAAGAAGGTCGAAAATACTGTGAAAAAACGAATAATTAATTATAGACTTAAGATTGACAA
TCTGCTTGCCAATCCTGATA

Product: melibiose:sodium symporter

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: Melibiose permease; Melibiose transporter; Na+ (Li+)/melibiose symporter; Thiomethylgalactoside permease II [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKLKTGQKCAFGFGAFGKDIVYMLISSYLLYYYNVVLGMSSVFIGTVMMAARIFDAFNDPVMGIIVAKTRTRWGRFRPWI
FAGTVLNAVTIYALYATPESMGDSAQRVWLTVFYFAWGITYTLMDIPFWSMIPAITEPGSDREQLTSLARTCSGIGDAIP
TVLTMTVVPILGAGSSMADYRIGFKWWALIIAVVFVISELVCVACVPERLVACDEKPGKISDMFKALFKNDQAMTVVVSI
ILVYTSLNICGNLVLYFFRFDVGNEGAYSLFAAVAFAAQVLVMMVIPLLRKRFSKSQLFISGFIIQIAGFAVILIMAFAK
LYTPQSWYILCLPGILIYLGYGMLNVIMTIFLSDSVDYGEVMNGTREESVIFSMQTFTVKLASGVAVFLAGLVVDWIHLD
TAAAVQTQDTLDALRMWMTIPSVILLIVGIFVFKRFYKLDDTRMEEIKSKLN

Sequences:

>Translated_452_residues
MKLKTGQKCAFGFGAFGKDIVYMLISSYLLYYYNVVLGMSSVFIGTVMMAARIFDAFNDPVMGIIVAKTRTRWGRFRPWI
FAGTVLNAVTIYALYATPESMGDSAQRVWLTVFYFAWGITYTLMDIPFWSMIPAITEPGSDREQLTSLARTCSGIGDAIP
TVLTMTVVPILGAGSSMADYRIGFKWWALIIAVVFVISELVCVACVPERLVACDEKPGKISDMFKALFKNDQAMTVVVSI
ILVYTSLNICGNLVLYFFRFDVGNEGAYSLFAAVAFAAQVLVMMVIPLLRKRFSKSQLFISGFIIQIAGFAVILIMAFAK
LYTPQSWYILCLPGILIYLGYGMLNVIMTIFLSDSVDYGEVMNGTREESVIFSMQTFTVKLASGVAVFLAGLVVDWIHLD
TAAAVQTQDTLDALRMWMTIPSVILLIVGIFVFKRFYKLDDTRMEEIKSKLN
>Mature_452_residues
MKLKTGQKCAFGFGAFGKDIVYMLISSYLLYYYNVVLGMSSVFIGTVMMAARIFDAFNDPVMGIIVAKTRTRWGRFRPWI
FAGTVLNAVTIYALYATPESMGDSAQRVWLTVFYFAWGITYTLMDIPFWSMIPAITEPGSDREQLTSLARTCSGIGDAIP
TVLTMTVVPILGAGSSMADYRIGFKWWALIIAVVFVISELVCVACVPERLVACDEKPGKISDMFKALFKNDQAMTVVVSI
ILVYTSLNICGNLVLYFFRFDVGNEGAYSLFAAVAFAAQVLVMMVIPLLRKRFSKSQLFISGFIIQIAGFAVILIMAFAK
LYTPQSWYILCLPGILIYLGYGMLNVIMTIFLSDSVDYGEVMNGTREESVIFSMQTFTVKLASGVAVFLAGLVVDWIHLD
TAAAVQTQDTLDALRMWMTIPSVILLIVGIFVFKRFYKLDDTRMEEIKSKLN

Specific function: Responsible for melibiose transport. It is capable of using hydrogen and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system) [H]

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:galactoside symporter (TC 2.A.2) family [H]

Homologues:

Organism=Escherichia coli, GI1790561, Length=451, Percent_Identity=32.3725055432373, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI48994989, Length=462, Percent_Identity=25.3246753246753, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87082306, Length=453, Percent_Identity=24.7240618101545, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786466, Length=451, Percent_Identity=23.5033259423503, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI145693206, Length=462, Percent_Identity=22.7272727272727, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787902, Length=449, Percent_Identity=23.3853006681514, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 50364; Mature: 50364

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKTGQKCAFGFGAFGKDIVYMLISSYLLYYYNVVLGMSSVFIGTVMMAARIFDAFNDP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VMGIIVAKTRTRWGRFRPWIFAGTVLNAVTIYALYATPESMGDSAQRVWLTVFYFAWGIT
EEEEEEEECHHCCCCCCCHHHHHHHHHHHHHHHEEECCHHHCCHHHHHHHHHHHHHHHHH
YTLMDIPFWSMIPAITEPGSDREQLTSLARTCSGIGDAIPTVLTMTVVPILGAGSSMADY
HHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RIGFKWWALIIAVVFVISELVCVACVPERLVACDEKPGKISDMFKALFKNDQAMTVVVSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHH
ILVYTSLNICGNLVLYFFRFDVGNEGAYSLFAAVAFAAQVLVMMVIPLLRKRFSKSQLFI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SGFIIQIAGFAVILIMAFAKLYTPQSWYILCLPGILIYLGYGMLNVIMTIFLSDSVDYGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VMNGTREESVIFSMQTFTVKLASGVAVFLAGLVVDWIHLDTAAAVQTQDTLDALRMWMTI
HHCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
PSVILLIVGIFVFKRFYKLDDTRMEEIKSKLN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MKLKTGQKCAFGFGAFGKDIVYMLISSYLLYYYNVVLGMSSVFIGTVMMAARIFDAFNDP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VMGIIVAKTRTRWGRFRPWIFAGTVLNAVTIYALYATPESMGDSAQRVWLTVFYFAWGIT
EEEEEEEECHHCCCCCCCHHHHHHHHHHHHHHHEEECCHHHCCHHHHHHHHHHHHHHHHH
YTLMDIPFWSMIPAITEPGSDREQLTSLARTCSGIGDAIPTVLTMTVVPILGAGSSMADY
HHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RIGFKWWALIIAVVFVISELVCVACVPERLVACDEKPGKISDMFKALFKNDQAMTVVVSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHH
ILVYTSLNICGNLVLYFFRFDVGNEGAYSLFAAVAFAAQVLVMMVIPLLRKRFSKSQLFI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SGFIIQIAGFAVILIMAFAKLYTPQSWYILCLPGILIYLGYGMLNVIMTIFLSDSVDYGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VMNGTREESVIFSMQTFTVKLASGVAVFLAGLVVDWIHLDTAAAVQTQDTLDALRMWMTI
HHCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
PSVILLIVGIFVFKRFYKLDDTRMEEIKSKLN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9188803 [H]