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The map label for this gene is smpB [H]

Identifier: 238923911

GI number: 238923911

Start: 1423241

End: 1423708

Strand: Direct

Name: smpB [H]

Synonym: EUBREC_1539

Alternate gene names: 238923911

Gene position: 1423241-1423708 (Clockwise)

Preceding gene: 238923910

Following gene: 238923912

Centisome position: 41.26

GC content: 41.24

Gene sequence:

>468_bases
ATGGCTAAGGAACCAAAGAAATTAATAGCCAACAACAAAAAGGTCTATCACGATTACTTTTTGCTTGAGAAATACGAAGC
CGGTATTTCACTTGCAGGAACTGAGGTGAAGTCACTTCGAATGGGAAAGGGCAGCATAAAGGAGTCATTCATCCGTATTG
AGAACGGTGAGATTATCCTGTATGGCATGCATATCACACCATATGAGAAGGGAAATATCTTCAACAAGGATCCACTCAGA
CCTAAAAAGCTTTTGCTCCACAAGAAGGAAATCGAGAAGCTTTCATCAAAGATGAAGGAAAAGGGTTTCACAATTGTGCC
GGTGGAGGTATACTTTAAGGGGAGTCTGGTCAAAGTAGAGATAGCACTTGCCAAAGGTAAGAAGCTTTATGATAAGAGAG
CAGACATAGCCAAGAAGGACCAAAAGCGAGAGGCACAGAGAGATTTCAAAATCAGAAATCTTGGTTAG

Upstream 100 bases:

>100_bases
ATGTGAACCAGCGGTTTACAATAATTTACGTTTTAGATATTGGCAATTGTAAAAAAATGTGTTAGTATTGTAAAGCAGTT
TGAGGAAAGGAGATACCGAC

Downstream 100 bases:

>100_bases
CAAAAAATGAATATGGGCTAGTAAAGGTTTCGACGGGGTACATGCAGCTGGAGAAGCGAGTCGTATGGCGATGCGTTAAA
TGGCCAAATTAAAATTAAAC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MAKEPKKLIANNKKVYHDYFLLEKYEAGISLAGTEVKSLRMGKGSIKESFIRIENGEIILYGMHITPYEKGNIFNKDPLR
PKKLLLHKKEIEKLSSKMKEKGFTIVPVEVYFKGSLVKVEIALAKGKKLYDKRADIAKKDQKREAQRDFKIRNLG

Sequences:

>Translated_155_residues
MAKEPKKLIANNKKVYHDYFLLEKYEAGISLAGTEVKSLRMGKGSIKESFIRIENGEIILYGMHITPYEKGNIFNKDPLR
PKKLLLHKKEIEKLSSKMKEKGFTIVPVEVYFKGSLVKVEIALAKGKKLYDKRADIAKKDQKREAQRDFKIRNLG
>Mature_154_residues
AKEPKKLIANNKKVYHDYFLLEKYEAGISLAGTEVKSLRMGKGSIKESFIRIENGEIILYGMHITPYEKGNIFNKDPLRP
KKLLLHKKEIEKLSSKMKEKGFTIVPVEVYFKGSLVKVEIALAKGKKLYDKRADIAKKDQKREAQRDFKIRNLG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=141, Percent_Identity=43.2624113475177, Blast_Score=124, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17870; Mature: 17739

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEPKKLIANNKKVYHDYFLLEKYEAGISLAGTEVKSLRMGKGSIKESFIRIENGEIIL
CCCCCHHHHHCCCEEEHHHHHEEHHCCCEEECCCHHHHEECCCCCCHHHEEEEECCCEEE
YGMHITPYEKGNIFNKDPLRPKKLLLHKKEIEKLSSKMKEKGFTIVPVEVYFKGSLVKVE
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEE
IALAKGKKLYDKRADIAKKDQKREAQRDFKIRNLG
EEEECCCHHHHHHHHHHHHHHHHHHHHCCEECCCC
>Mature Secondary Structure 
AKEPKKLIANNKKVYHDYFLLEKYEAGISLAGTEVKSLRMGKGSIKESFIRIENGEIIL
CCCCHHHHHCCCEEEHHHHHEEHHCCCEEECCCHHHHEECCCCCCHHHEEEEECCCEEE
YGMHITPYEKGNIFNKDPLRPKKLLLHKKEIEKLSSKMKEKGFTIVPVEVYFKGSLVKVE
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEE
IALAKGKKLYDKRADIAKKDQKREAQRDFKIRNLG
EEEECCCHHHHHHHHHHHHHHHHHHHHCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA