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The map label for this gene is ispF

Identifier: 238923818

GI number: 238923818

Start: 1325463

End: 1325996

Strand: Direct

Name: ispF

Synonym: EUBREC_1445

Alternate gene names: 238923818

Gene position: 1325463-1325996 (Clockwise)

Preceding gene: 238923816

Following gene: 238923819

Centisome position: 38.42

GC content: 49.44

Gene sequence:

>534_bases
ATGAGAGTAGGAATGGGCTATGATGTGCACAAGCTTGTTGAGGGCAGAGATTTGATTTTAGGAGGAGTGAAGGTTCCGCA
TACACTCGGGCTTTTAGGTCATTCGGATGCCGATGTACTGTTGCATGCGATAATGGATGCACTCTTGGGTGCAGCGGCAC
TTGGAGATATCGGAAAGCATTTCCCGGATACAGACCCGGCATATGAGGGTATTTCGAGTCTTAAGCTTTTGGAGCATGTG
AGAGACCTCATTGCAAAAAAAGGCTATGTGATAGAAAACATAGATGCGACAGTAATTGCACAAAAGCCGAAGCTTCGTCC
GTATATTGAGCAGATGGAGCAGAATGTGGCTGATACGCTGCAAATCGCAAAGGAGCAGGTAAATATAAAGGCGACGACCG
AGGAGTGGCTTGGTTTTACAGGCCGCGAGGAGGGGATTGCATCACAGGCAATCTGCTCACTTACAGGTCTCTATGAAGCA
AGTATGCAGGTCGGAGGCGGATGCAGCGGCTGCGGAGGCTGTCAGGCCGATTGA

Upstream 100 bases:

>100_bases
AGGAGAAATAAGGTATACTATATTTAAAAAACCAAAATACAGAGCAATGACAGTTGGCAATAACATGAGGTATTGTATCT
GATAGAAAGAGAGACGATAT

Downstream 100 bases:

>100_bases
TTTGAATATATAGGGAAAACATGCTAAAATAAAAAGATAGTTATAAATCATCCAAAAGGAGATATAAATAATGGAGAACA
ATTTCAAGTTTTACAATACA

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MRVGMGYDVHKLVEGRDLILGGVKVPHTLGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDPAYEGISSLKLLEHV
RDLIAKKGYVIENIDATVIAQKPKLRPYIEQMEQNVADTLQIAKEQVNIKATTEEWLGFTGREEGIASQAICSLTGLYEA
SMQVGGGCSGCGGCQAD

Sequences:

>Translated_177_residues
MRVGMGYDVHKLVEGRDLILGGVKVPHTLGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDPAYEGISSLKLLEHV
RDLIAKKGYVIENIDATVIAQKPKLRPYIEQMEQNVADTLQIAKEQVNIKATTEEWLGFTGREEGIASQAICSLTGLYEA
SMQVGGGCSGCGGCQAD
>Mature_177_residues
MRVGMGYDVHKLVEGRDLILGGVKVPHTLGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDPAYEGISSLKLLEHV
RDLIAKKGYVIENIDATVIAQKPKLRPYIEQMEQNVADTLQIAKEQVNIKATTEEWLGFTGREEGIASQAICSLTGLYEA
SMQVGGGCSGCGGCQAD

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=55.1948051948052, Blast_Score=161, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_EUBR3 (C4Z8W9)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002937334.1
- GeneID:   7963811
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_1445
- ProtClustDB:   CLSK2506399
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 18858; Mature: 18858

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGMGYDVHKLVEGRDLILGGVKVPHTLGLLGHSDADVLLHAIMDALLGAAALGDIGKH
CCCCCCCCHHHHHCCCCEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPDTDPAYEGISSLKLLEHVRDLIAKKGYVIENIDATVIAQKPKLRPYIEQMEQNVADTL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHH
QIAKEQVNIKATTEEWLGFTGREEGIASQAICSLTGLYEASMQVGGGCSGCGGCQAD
HHHHHHHCCEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVGMGYDVHKLVEGRDLILGGVKVPHTLGLLGHSDADVLLHAIMDALLGAAALGDIGKH
CCCCCCCCHHHHHCCCCEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPDTDPAYEGISSLKLLEHVRDLIAKKGYVIENIDATVIAQKPKLRPYIEQMEQNVADTL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHH
QIAKEQVNIKATTEEWLGFTGREEGIASQAICSLTGLYEASMQVGGGCSGCGGCQAD
HHHHHHHCCEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA