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The map label for this gene is mdeA [H]

Identifier: 238923815

GI number: 238923815

Start: 1322725

End: 1324035

Strand: Direct

Name: mdeA [H]

Synonym: EUBREC_1442

Alternate gene names: 238923815

Gene position: 1322725-1324035 (Clockwise)

Preceding gene: 238923814

Following gene: 238923816

Centisome position: 38.34

GC content: 43.63

Gene sequence:

>1311_bases
ATGAGCAAGAAACATTATGCAGACAGAAATTTAAAATTTGAGACATTACAGTTACATGTAGGACAGGAAACACCTGATCC
GGTTACAGATGCGAGAGCAGTTCCAATCTACCTTACTTCATCATATGTGTTCCATAACAGTGAGCATGCGGCAGACAGAT
TCGGACTCAGAGATGCAGGAAATATCTACGGCAGACTGACAAACCCTACAGAGGATGTATTTGAGAGAAGAATTGCAGCA
CTCGAGGGTGGCGTGGCAGCACTTGCTGTTGGTTCCGGAGCAGCGGCACTCACATATGCTTTCCAGGCAGTAGCACACGC
AGGTGACCATATCGTTGCAGCAAAGAATATCTACGGCGGTACATACAATCTCTTGGCACACACACTTCCTGATTACGGAA
TTGAGGCTACATTCGTAGATCCATTCGATTATGATGGAATCAAGGCTGCCATAAAAGAGAATACAAAAGCTATTGAAATA
GAGACACTCGGAAATCCAAACTCAGAGGTGGTAGATATCGAAAAAATTGCAAATATCGCTCATGAGGCAGGAATCCCACT
TATCGTAGATAATACCTTTGCTACACCATATCTTGTCCGTCCGATTGAGTACGGTGCTGATATCGTGGTTCACTCAGCAA
CAAAGTTCATCGGTGGACACGGAACAACAATTGGTGGAGTTATAATTGACAGCGGTAAATTCGACTGGACACAGAATGAT
AAATGGCCATGGCTTGTAGAGCCAAACGTATCATACCACGGAGTAAGCTTTGCAAAGGATTGCGCACCGGCAGCATTTGC
GACATATATCAGGGCAATTTTACTTCGTGATACAGGAGCAACAATTTCACCTGTTCATTCATTTATCTTCCTTCAGGGAC
TTGAGACACTTTCGCTTCGTGTGGAGCGTCATGTTGAGAATGCACTCAAGGTAGTTCAGTACTTAAAGGATCAGCCACTC
GTAGAGAAGGTTAATCATCCATCTATTTCAGAGGATAAAGAGCAGCAGGAGCTTTACAAGAAATACTTCCATAACGGAGG
CGGTTCTATATTTACATTCGAGATTAAGGGTGGAGCAAAGGAAGCGCAGAAATTTATTGACAACCTTGAGCTTTTCTCAC
TGCTTGCAAACGTAGCTGATGTTAAGTCACTTGCTATACATCCGGCTTCTACCACACACTCAGAGTGCAATGAGGCAGAA
CTCTTAGATCAGGGCATCAAGCCAAACACAATCCGTCTTTCAATCGGAACTGAGAATGTGGATGATATAATTGAGGATCT
TGATGAGGCATTTAAGGCACTTGCAGAGTAA

Upstream 100 bases:

>100_bases
TAGAAAGATGATATGATATGATTAGTTCAAGGGAACAGCATCCCGGAATTAAAATTAAAAACAAATAACATTAACATATC
ATTAATAGGAGGATATTAAT

Downstream 100 bases:

>100_bases
ACGGTTTTGTATGATAAATACTCCTTAAACGAATAAATAGTGATCATTATAAAAGGAATCGGAAAATATTAATCCGGTTC
CTTTTATTGTGCCATGAAAT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSKKHYADRNLKFETLQLHVGQETPDPVTDARAVPIYLTSSYVFHNSEHAADRFGLRDAGNIYGRLTNPTEDVFERRIAA
LEGGVAALAVGSGAAALTYAFQAVAHAGDHIVAAKNIYGGTYNLLAHTLPDYGIEATFVDPFDYDGIKAAIKENTKAIEI
ETLGNPNSEVVDIEKIANIAHEAGIPLIVDNTFATPYLVRPIEYGADIVVHSATKFIGGHGTTIGGVIIDSGKFDWTQND
KWPWLVEPNVSYHGVSFAKDCAPAAFATYIRAILLRDTGATISPVHSFIFLQGLETLSLRVERHVENALKVVQYLKDQPL
VEKVNHPSISEDKEQQELYKKYFHNGGGSIFTFEIKGGAKEAQKFIDNLELFSLLANVADVKSLAIHPASTTHSECNEAE
LLDQGIKPNTIRLSIGTENVDDIIEDLDEAFKALAE

Sequences:

>Translated_436_residues
MSKKHYADRNLKFETLQLHVGQETPDPVTDARAVPIYLTSSYVFHNSEHAADRFGLRDAGNIYGRLTNPTEDVFERRIAA
LEGGVAALAVGSGAAALTYAFQAVAHAGDHIVAAKNIYGGTYNLLAHTLPDYGIEATFVDPFDYDGIKAAIKENTKAIEI
ETLGNPNSEVVDIEKIANIAHEAGIPLIVDNTFATPYLVRPIEYGADIVVHSATKFIGGHGTTIGGVIIDSGKFDWTQND
KWPWLVEPNVSYHGVSFAKDCAPAAFATYIRAILLRDTGATISPVHSFIFLQGLETLSLRVERHVENALKVVQYLKDQPL
VEKVNHPSISEDKEQQELYKKYFHNGGGSIFTFEIKGGAKEAQKFIDNLELFSLLANVADVKSLAIHPASTTHSECNEAE
LLDQGIKPNTIRLSIGTENVDDIIEDLDEAFKALAE
>Mature_435_residues
SKKHYADRNLKFETLQLHVGQETPDPVTDARAVPIYLTSSYVFHNSEHAADRFGLRDAGNIYGRLTNPTEDVFERRIAAL
EGGVAALAVGSGAAALTYAFQAVAHAGDHIVAAKNIYGGTYNLLAHTLPDYGIEATFVDPFDYDGIKAAIKENTKAIEIE
TLGNPNSEVVDIEKIANIAHEAGIPLIVDNTFATPYLVRPIEYGADIVVHSATKFIGGHGTTIGGVIIDSGKFDWTQNDK
WPWLVEPNVSYHGVSFAKDCAPAAFATYIRAILLRDTGATISPVHSFIFLQGLETLSLRVERHVENALKVVQYLKDQPLV
EKVNHPSISEDKEQQELYKKYFHNGGGSIFTFEIKGGAKEAQKFIDNLELFSLLANVADVKSLAIHPASTTHSECNEAEL
LDQGIKPNTIRLSIGTENVDDIIEDLDEAFKALAE

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=425, Percent_Identity=34.5882352941176, Blast_Score=208, Evalue=8e-54,
Organism=Homo sapiens, GI299473759, Length=425, Percent_Identity=31.7647058823529, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI25453487, Length=424, Percent_Identity=29.4811320754717, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1790375, Length=401, Percent_Identity=32.6683291770574, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1789383, Length=412, Percent_Identity=26.9417475728155, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71980708, Length=433, Percent_Identity=32.1016166281755, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17538055, Length=423, Percent_Identity=34.7517730496454, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=423, Percent_Identity=33.3333333333333, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=48.735632183908, Blast_Score=348, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6319307, Length=426, Percent_Identity=32.1596244131455, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321170, Length=362, Percent_Identity=27.6243093922652, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321904, Length=421, Percent_Identity=25.41567695962, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321254, Length=421, Percent_Identity=25.1781472684086, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=34.9881796690307, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47690; Mature: 47559

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKHYADRNLKFETLQLHVGQETPDPVTDARAVPIYLTSSYVFHNSEHAADRFGLRDAG
CCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEEEEEEECCCHHHHHCCCCCCC
NIYGRLTNPTEDVFERRIAALEGGVAALAVGSGAAALTYAFQAVAHAGDHIVAAKNIYGG
CCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCC
TYNLLAHTLPDYGIEATFVDPFDYDGIKAAIKENTKAIEIETLGNPNSEVVDIEKIANIA
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCEEEEEEECCCCCCCEEEHHHHHHHH
HEAGIPLIVDNTFATPYLVRPIEYGADIVVHSATKFIGGHGTTIGGVIIDSGKFDWTQND
HHCCCCEEEECCCCCCEEECCHHCCCCEEEECHHHHHCCCCCEECEEEEECCCCCCCCCC
KWPWLVEPNVSYHGVSFAKDCAPAAFATYIRAILLRDTGATISPVHSFIFLQGLETLSLR
CCCEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VERHVENALKVVQYLKDQPLVEKVNHPSISEDKEQQELYKKYFHNGGGSIFTFEIKGGAK
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHH
EAQKFIDNLELFSLLANVADVKSLAIHPASTTHSECNEAELLDQGIKPNTIRLSIGTENV
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCH
DDIIEDLDEAFKALAE
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKKHYADRNLKFETLQLHVGQETPDPVTDARAVPIYLTSSYVFHNSEHAADRFGLRDAG
CCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEEEEEEECCCHHHHHCCCCCCC
NIYGRLTNPTEDVFERRIAALEGGVAALAVGSGAAALTYAFQAVAHAGDHIVAAKNIYGG
CCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCC
TYNLLAHTLPDYGIEATFVDPFDYDGIKAAIKENTKAIEIETLGNPNSEVVDIEKIANIA
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCEEEEEEECCCCCCCEEEHHHHHHHH
HEAGIPLIVDNTFATPYLVRPIEYGADIVVHSATKFIGGHGTTIGGVIIDSGKFDWTQND
HHCCCCEEEECCCCCCEEECCHHCCCCEEEECHHHHHCCCCCEECEEEEECCCCCCCCCC
KWPWLVEPNVSYHGVSFAKDCAPAAFATYIRAILLRDTGATISPVHSFIFLQGLETLSLR
CCCEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VERHVENALKVVQYLKDQPLVEKVNHPSISEDKEQQELYKKYFHNGGGSIFTFEIKGGAK
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHH
EAQKFIDNLELFSLLANVADVKSLAIHPASTTHSECNEAELLDQGIKPNTIRLSIGTENV
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCH
DDIIEDLDEAFKALAE
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]