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The map label for this gene is metH [H]

Identifier: 238923813

GI number: 238923813

Start: 1318543

End: 1320993

Strand: Direct

Name: metH [H]

Synonym: EUBREC_1436

Alternate gene names: 238923813

Gene position: 1318543-1320993 (Clockwise)

Preceding gene: 238923810

Following gene: 238923814

Centisome position: 38.22

GC content: 46.67

Gene sequence:

>2451_bases
ATGACAAGAGAAGAATTCAGAAAATATATCAGAGAAAACATAGTTATCCTGGATGGAGCGACCGGCACAAACCTGATGGC
AGCAGGCATGCCGGTAGGAGTGTGTCCGGAGGAATGGGTGATGGAGCATCCGCAGGTGATTCTTGATTTGCAGAAGGCTT
ATGTCGATAACGGCACGAATATTGTTTATGCACCTACATTTACAGGAAACAGGATTAAGCTTTTAGAATATGGTTTACAG
GAGAAAATTAACGAGATAAATACCACACTGGTTGGCCTGTCAAAGCAGGCTGTCGGGGATAGGGCACTTGTAGCAGCAGA
TATGACGATGACCGGCAGACAGCTTTTTCCACTCGGGGATCTGATGTTTGAGGAGCTTTTGGAGGTGTACAAGGAGCAGG
CGAGGATTCTTGATGAGGCGGGCGCAGATGTATTTGTGGTTGAGACAATGATGAGTCTTCAGGAGTGCAGAGCGGCAGTG
CTTGCGATAAAAGAGGTAAGCGACCTGCCTATTATGGTGACACTCACCTACAACGAGGATGGCAGGACTCTTTTTGGTAC
ACCGCCTGAGACAGCAGTTGTTGTGCTGCAGTCACTTGGAGTGGATGCAATCGGTGTGAACTGCTCGACGGGACCTGCTG
AGATGATAGCTCTGGTTGAGAAAATGGCAGAGTACTCTACAGTGCCTCTTATTGCAAAGCCAAATGCCGGGCTTCCGGAG
CTTGAGGATGGTAAAACTGTATACAAGATGACTCCGGATATGTTTGCCGATGCGATGAGAGGGCTTGTGAAGGCAGGTGC
ATCCATTGTAGGAGGCTGCTGTGGCACCACGCCGGAGCATATCCGTGCACTGACAGAGGCTGTAAAGACAATGCCTGTTC
ATAAGCCGCTTGAGCATCATAGAAGAGTGCTTGCGTCTGAACGAAAGAATGTAGAGATTGACTTAGATGGCAACTTTACC
GTTGTCGGAGAGAGAATCAATCCTACAGGCAAGAAAAAGCTGCAGGCACAGCTTCGTGAGGGAAAGCTTGACCTGGTGCG
CCAGATGGCTATGGAGCAGGAGACAAATGGTGCGGGAATACTTGATATCAATATGGGCATGAATGGTATCGATGAGAAGG
AGATGATGAAATCGGTTATATATGAGGTTTCATCAACAGTGGACTGCCCTCTTTGTATTGATTCAAGCTATGTGGAGGTA
ATAGAGGAGGCTCTTAAGATTTATCCGGGAAGAGCTCTCATCAATTCTATTTCCCTTGAGACAGAAAAGATGGAAAAGCT
TCTGCCTCTTGCAGCAAAATATGGGGCGATGTTCATACTCTTACCACTTTCGGATGCAGGATTGCCAAAGGATGTCGAGG
AGAAAAAGCAGATTATAAATACTATTTATGACAAGGCACTTTCGCTTGGAATGGCACATGAGGATATAGTGGTGGATGGA
CTTGTTGCGACAATAGGTGCAAATCCAAAGGCCGCAATCGAGTGCTATGAGACCATTGCGTACTGTAAGGATGAGAAAAA
GCTTCCTACAATATGCGGCCTTTCAAATATATCCTTCGGACTGCCTGAGCGTATGTACGTGAACACAGCGTTTCTTACAA
TGGCCATATGCAAGGGACTCACCATGGCTATTGCCAATCCATCACAGGAGCTTTTGATGAATGCTGCATTTGCATCGGAT
ATGCTGCTCGACAGACCTGACAGCGATATAGCATACATCGAGCGCATGAGCAGGCTTGCGGAGGAAAAGGCCCAATATGA
GACTGTTGTTGTGAAAAAGTCGGACAATGATGCGTCTGCTTCAAACGGCACATGCGCAGCGGGCAGTAAGGATGCGGTAT
TTCAGGCTGTCTTAAAGGGAAACAAGGGCAGTATAATTGATGAAGTAAAGAAAATGCTTTCTGATGGGGCAAAGCCTGAC
GAGATTATTAATAACAGCCTGATTCCGGCTATTAATGAGGTGGGAGAGCTTTTCAACCAGAAGAAATATTTCCTGCCACA
GCTTATCGGCAGTGCAAATACCATGAAGCTTGCTATAGAGCATCTGGAGCCGTTACTTGAAAAAAAGGACTCGGGTGATG
ATATGCCGACTCTTGTCATTGCAACGGTGGAGGGCGATATTCATGATATAGGAAAGAACCTTGTCGTGCTTATGCTTAAA
AACTATGGCTACAACGTGATTGATATGGGAAAGGATGTGCCTTGTGAGGATATCGTCAACAAGGCGATTGAGACGAACGC
GGCTGTTATCGGACTGTCAGCTCTTATGACCACCACCATGATGCGCATGAAGGATGTAGTGGAAATATGTAAGGAAAAGG
GCTGTAAGAGCAAGGTTGTAATCGGTGGAGCCTGCATTACACAAAGCTTTGCAGACGAGATAGGAGCCGACGGATATTCA
AAGGATGCGGCAGAGTGCGTGAAGCTTGTGGAGAGACTTCTGAATAGTTAA

Upstream 100 bases:

>100_bases
TAAATTATCACAAATTTCAAGGATGAAGAGCACATAGCTTTTGCTTTGAAATATTTTACACTGTGGAGTGAATAACAGCT
GAAACAGAAAAGGAAACGAA

Downstream 100 bases:

>100_bases
AAATTTTTTTAATAAAATAAAAAAAAGTTGTTGACAATAAGGTGCCGACTGTATATAATAATCAAGTCGGTTGACGACAT
GGGATCTTAGCTCAGCTGGG

Product: 5-methyltetrahydrofolate--homocysteine methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 816; Mature: 815

Protein sequence:

>816_residues
MTREEFRKYIRENIVILDGATGTNLMAAGMPVGVCPEEWVMEHPQVILDLQKAYVDNGTNIVYAPTFTGNRIKLLEYGLQ
EKINEINTTLVGLSKQAVGDRALVAADMTMTGRQLFPLGDLMFEELLEVYKEQARILDEAGADVFVVETMMSLQECRAAV
LAIKEVSDLPIMVTLTYNEDGRTLFGTPPETAVVVLQSLGVDAIGVNCSTGPAEMIALVEKMAEYSTVPLIAKPNAGLPE
LEDGKTVYKMTPDMFADAMRGLVKAGASIVGGCCGTTPEHIRALTEAVKTMPVHKPLEHHRRVLASERKNVEIDLDGNFT
VVGERINPTGKKKLQAQLREGKLDLVRQMAMEQETNGAGILDINMGMNGIDEKEMMKSVIYEVSSTVDCPLCIDSSYVEV
IEEALKIYPGRALINSISLETEKMEKLLPLAAKYGAMFILLPLSDAGLPKDVEEKKQIINTIYDKALSLGMAHEDIVVDG
LVATIGANPKAAIECYETIAYCKDEKKLPTICGLSNISFGLPERMYVNTAFLTMAICKGLTMAIANPSQELLMNAAFASD
MLLDRPDSDIAYIERMSRLAEEKAQYETVVVKKSDNDASASNGTCAAGSKDAVFQAVLKGNKGSIIDEVKKMLSDGAKPD
EIINNSLIPAINEVGELFNQKKYFLPQLIGSANTMKLAIEHLEPLLEKKDSGDDMPTLVIATVEGDIHDIGKNLVVLMLK
NYGYNVIDMGKDVPCEDIVNKAIETNAAVIGLSALMTTTMMRMKDVVEICKEKGCKSKVVIGGACITQSFADEIGADGYS
KDAAECVKLVERLLNS

Sequences:

>Translated_816_residues
MTREEFRKYIRENIVILDGATGTNLMAAGMPVGVCPEEWVMEHPQVILDLQKAYVDNGTNIVYAPTFTGNRIKLLEYGLQ
EKINEINTTLVGLSKQAVGDRALVAADMTMTGRQLFPLGDLMFEELLEVYKEQARILDEAGADVFVVETMMSLQECRAAV
LAIKEVSDLPIMVTLTYNEDGRTLFGTPPETAVVVLQSLGVDAIGVNCSTGPAEMIALVEKMAEYSTVPLIAKPNAGLPE
LEDGKTVYKMTPDMFADAMRGLVKAGASIVGGCCGTTPEHIRALTEAVKTMPVHKPLEHHRRVLASERKNVEIDLDGNFT
VVGERINPTGKKKLQAQLREGKLDLVRQMAMEQETNGAGILDINMGMNGIDEKEMMKSVIYEVSSTVDCPLCIDSSYVEV
IEEALKIYPGRALINSISLETEKMEKLLPLAAKYGAMFILLPLSDAGLPKDVEEKKQIINTIYDKALSLGMAHEDIVVDG
LVATIGANPKAAIECYETIAYCKDEKKLPTICGLSNISFGLPERMYVNTAFLTMAICKGLTMAIANPSQELLMNAAFASD
MLLDRPDSDIAYIERMSRLAEEKAQYETVVVKKSDNDASASNGTCAAGSKDAVFQAVLKGNKGSIIDEVKKMLSDGAKPD
EIINNSLIPAINEVGELFNQKKYFLPQLIGSANTMKLAIEHLEPLLEKKDSGDDMPTLVIATVEGDIHDIGKNLVVLMLK
NYGYNVIDMGKDVPCEDIVNKAIETNAAVIGLSALMTTTMMRMKDVVEICKEKGCKSKVVIGGACITQSFADEIGADGYS
KDAAECVKLVERLLNS
>Mature_815_residues
TREEFRKYIRENIVILDGATGTNLMAAGMPVGVCPEEWVMEHPQVILDLQKAYVDNGTNIVYAPTFTGNRIKLLEYGLQE
KINEINTTLVGLSKQAVGDRALVAADMTMTGRQLFPLGDLMFEELLEVYKEQARILDEAGADVFVVETMMSLQECRAAVL
AIKEVSDLPIMVTLTYNEDGRTLFGTPPETAVVVLQSLGVDAIGVNCSTGPAEMIALVEKMAEYSTVPLIAKPNAGLPEL
EDGKTVYKMTPDMFADAMRGLVKAGASIVGGCCGTTPEHIRALTEAVKTMPVHKPLEHHRRVLASERKNVEIDLDGNFTV
VGERINPTGKKKLQAQLREGKLDLVRQMAMEQETNGAGILDINMGMNGIDEKEMMKSVIYEVSSTVDCPLCIDSSYVEVI
EEALKIYPGRALINSISLETEKMEKLLPLAAKYGAMFILLPLSDAGLPKDVEEKKQIINTIYDKALSLGMAHEDIVVDGL
VATIGANPKAAIECYETIAYCKDEKKLPTICGLSNISFGLPERMYVNTAFLTMAICKGLTMAIANPSQELLMNAAFASDM
LLDRPDSDIAYIERMSRLAEEKAQYETVVVKKSDNDASASNGTCAAGSKDAVFQAVLKGNKGSIIDEVKKMLSDGAKPDE
IINNSLIPAINEVGELFNQKKYFLPQLIGSANTMKLAIEHLEPLLEKKDSGDDMPTLVIATVEGDIHDIGKNLVVLMLKN
YGYNVIDMGKDVPCEDIVNKAIETNAAVIGLSALMTTTMMRMKDVVEICKEKGCKSKVVIGGACITQSFADEIGADGYSK
DAAECVKLVERLLNS

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=900, Percent_Identity=29, Blast_Score=296, Evalue=6e-80,
Organism=Homo sapiens, GI13162290, Length=292, Percent_Identity=27.0547945205479, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI157266337, Length=299, Percent_Identity=28.0936454849498, Blast_Score=79, Evalue=2e-14,
Organism=Escherichia coli, GI1790450, Length=881, Percent_Identity=30.1929625425653, Blast_Score=325, Evalue=7e-90,
Organism=Escherichia coli, GI1786456, Length=298, Percent_Identity=23.8255033557047, Blast_Score=63, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17535405, Length=867, Percent_Identity=29.9884659746251, Blast_Score=310, Evalue=2e-84,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 88824; Mature: 88693

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTREEFRKYIRENIVILDGATGTNLMAAGMPVGVCPEEWVMEHPQVILDLQKAYVDNGTN
CCHHHHHHHHHCCEEEEECCCCCCEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCE
IVYAPTFTGNRIKLLEYGLQEKINEINTTLVGLSKQAVGDRALVAADMTMTGRQLFPLGD
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCCCCCCCHHH
LMFEELLEVYKEQARILDEAGADVFVVETMMSLQECRAAVLAIKEVSDLPIMVTLTYNED
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
GRTLFGTPPETAVVVLQSLGVDAIGVNCSTGPAEMIALVEKMAEYSTVPLIAKPNAGLPE
CCEEECCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCC
LEDGKTVYKMTPDMFADAMRGLVKAGASIVGGCCGTTPEHIRALTEAVKTMPVHKPLEHH
CCCCCEEEEECHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
RRVLASERKNVEIDLDGNFTVVGERINPTGKKKLQAQLREGKLDLVRQMAMEQETNGAGI
HHHHHHCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEE
LDINMGMNGIDEKEMMKSVIYEVSSTVDCPLCIDSSYVEVIEEALKIYPGRALINSISLE
EEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHCC
TEKMEKLLPLAAKYGAMFILLPLSDAGLPKDVEEKKQIINTIYDKALSLGMAHEDIVVDG
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LVATIGANPKAAIECYETIAYCKDEKKLPTICGLSNISFGLPERMYVNTAFLTMAICKGL
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
TMAIANPSQELLMNAAFASDMLLDRPDSDIAYIERMSRLAEEKAQYETVVVKKSDNDASA
EEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
SNGTCAAGSKDAVFQAVLKGNKGSIIDEVKKMLSDGAKPDEIINNSLIPAINEVGELFNQ
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHCC
KKYFLPQLIGSANTMKLAIEHLEPLLEKKDSGDDMPTLVIATVEGDIHDIGKNLVVLMLK
CHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHCCHHEEEEHH
NYGYNVIDMGKDVPCEDIVNKAIETNAAVIGLSALMTTTMMRMKDVVEICKEKGCKSKVV
HCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IGGACITQSFADEIGADGYSKDAAECVKLVERLLNS
ECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TREEFRKYIRENIVILDGATGTNLMAAGMPVGVCPEEWVMEHPQVILDLQKAYVDNGTN
CHHHHHHHHHCCEEEEECCCCCCEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCE
IVYAPTFTGNRIKLLEYGLQEKINEINTTLVGLSKQAVGDRALVAADMTMTGRQLFPLGD
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCCCCCCCHHH
LMFEELLEVYKEQARILDEAGADVFVVETMMSLQECRAAVLAIKEVSDLPIMVTLTYNED
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
GRTLFGTPPETAVVVLQSLGVDAIGVNCSTGPAEMIALVEKMAEYSTVPLIAKPNAGLPE
CCEEECCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCC
LEDGKTVYKMTPDMFADAMRGLVKAGASIVGGCCGTTPEHIRALTEAVKTMPVHKPLEHH
CCCCCEEEEECHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
RRVLASERKNVEIDLDGNFTVVGERINPTGKKKLQAQLREGKLDLVRQMAMEQETNGAGI
HHHHHHCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEE
LDINMGMNGIDEKEMMKSVIYEVSSTVDCPLCIDSSYVEVIEEALKIYPGRALINSISLE
EEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHCC
TEKMEKLLPLAAKYGAMFILLPLSDAGLPKDVEEKKQIINTIYDKALSLGMAHEDIVVDG
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LVATIGANPKAAIECYETIAYCKDEKKLPTICGLSNISFGLPERMYVNTAFLTMAICKGL
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
TMAIANPSQELLMNAAFASDMLLDRPDSDIAYIERMSRLAEEKAQYETVVVKKSDNDASA
EEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
SNGTCAAGSKDAVFQAVLKGNKGSIIDEVKKMLSDGAKPDEIINNSLIPAINEVGELFNQ
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHCC
KKYFLPQLIGSANTMKLAIEHLEPLLEKKDSGDDMPTLVIATVEGDIHDIGKNLVVLMLK
CHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHCCHHEEEEHH
NYGYNVIDMGKDVPCEDIVNKAIETNAAVIGLSALMTTTMMRMKDVVEICKEKGCKSKVV
HCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IGGACITQSFADEIGADGYSKDAAECVKLVERLLNS
ECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]