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The map label for this gene is ydjE [H]

Identifier: 238923791

GI number: 238923791

Start: 1294478

End: 1295440

Strand: Direct

Name: ydjE [H]

Synonym: EUBREC_1413

Alternate gene names: 238923791

Gene position: 1294478-1295440 (Clockwise)

Preceding gene: 238923790

Following gene: 238923792

Centisome position: 37.52

GC content: 41.43

Gene sequence:

>963_bases
ATGGAAGAAAAAGTTGATATTGTGGCATTGGGGGAATTACTGATTGATTTTACGGAAGCAGGACACAGTCAGGGTGGGAG
AAAACTGTTTGAACAGAATCCCGGTGGTGCTCCGGCAAATCTTCTGACAGTTGCAAGTCATTTTGGGTATCGCACCTCTT
TTATTGGAAAAGTTGGAAACGATATGCATGGTAGATTTTTAAAGAGGACACTTCAAACGGAAGGAATCAATACAGATGCT
ATCGTCGAAGATCCGGATTATTTTACAACACTGGCATTTGTGGAAATCGGTGAAAATGGAGAAAGAAATTTTTCTTTTGC
AAGAAAACCGGGTGCAGATACACAGTTAAAAAAAGAAGAATTGGATCAGACGTTAATTTCCGGATGCAGAATTTTTCATT
TTGGATCATTATCGCTGACAGACGAGCCGGCAGAAAGCGCAACGATCGAAGCAGTAAAAATGGCAAAAGCAGCGGGTGTA
CTTATTTCATATGATCCAAATTACCGCCCGTCTCTTTGGAAGAGCAAAGAGTATGCAGTGAAAAAAATGAAGTCTGTTGT
AGAACTGGTAGATGTCATGAAAGTATCGGATGAAGAGAGTATTCTACTGACAGGGGCTAAAAGTTATGAGCAGGCTGCAG
AAGAAATTCTTGCTATGGGACCAAAGCTGGTTGCCATTACATTGGGAGAACATGGCGTTCTTATGGCAACAAAAAGCAGA
AAAGAAATCATCAAAGCATTTCAGACACATGCGGTCGATACGACCGGAGCAGGGGATTCTTTCTGGGGAGGCGTATTATG
CAGTATTCTTTCTATAAATAAGCCCGTAGAAAAGATGGAATGGGAAGAAATCAGAAAGTGTGCTGTTTTGGGAAATGCAG
TTGCAGGATTGTGTGTGCAAAAGCGAGGTGGAATACCTGCGATTCCGATGAAAGAGGCAGTGTTTGAATTTATGCAGAAA
TAA

Upstream 100 bases:

>100_bases
ACGACGATTCCAGGTGCACTTTGGATTGTGACTGCGGTTGTATTATTTTTCTACCGGTTAAATAAAAAACGTTATAACGA
GATTGTGGAGGAACTGAAAA

Downstream 100 bases:

>100_bases
TGAGTGTATGTTGCAATACTGGTTTCTACAGCAGTTTGTGATGTGCTGTCATACCTTGTGAAGTAGTATCTGGAAAATTG
ATGATTCCGGCTTGACTTAA

Product: pfkB-type carbohydrate kinase family protein

Products: ADP; D-fructose 6-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MEEKVDIVALGELLIDFTEAGHSQGGRKLFEQNPGGAPANLLTVASHFGYRTSFIGKVGNDMHGRFLKRTLQTEGINTDA
IVEDPDYFTTLAFVEIGENGERNFSFARKPGADTQLKKEELDQTLISGCRIFHFGSLSLTDEPAESATIEAVKMAKAAGV
LISYDPNYRPSLWKSKEYAVKKMKSVVELVDVMKVSDEESILLTGAKSYEQAAEEILAMGPKLVAITLGEHGVLMATKSR
KEIIKAFQTHAVDTTGAGDSFWGGVLCSILSINKPVEKMEWEEIRKCAVLGNAVAGLCVQKRGGIPAIPMKEAVFEFMQK

Sequences:

>Translated_320_residues
MEEKVDIVALGELLIDFTEAGHSQGGRKLFEQNPGGAPANLLTVASHFGYRTSFIGKVGNDMHGRFLKRTLQTEGINTDA
IVEDPDYFTTLAFVEIGENGERNFSFARKPGADTQLKKEELDQTLISGCRIFHFGSLSLTDEPAESATIEAVKMAKAAGV
LISYDPNYRPSLWKSKEYAVKKMKSVVELVDVMKVSDEESILLTGAKSYEQAAEEILAMGPKLVAITLGEHGVLMATKSR
KEIIKAFQTHAVDTTGAGDSFWGGVLCSILSINKPVEKMEWEEIRKCAVLGNAVAGLCVQKRGGIPAIPMKEAVFEFMQK
>Mature_320_residues
MEEKVDIVALGELLIDFTEAGHSQGGRKLFEQNPGGAPANLLTVASHFGYRTSFIGKVGNDMHGRFLKRTLQTEGINTDA
IVEDPDYFTTLAFVEIGENGERNFSFARKPGADTQLKKEELDQTLISGCRIFHFGSLSLTDEPAESATIEAVKMAKAAGV
LISYDPNYRPSLWKSKEYAVKKMKSVVELVDVMKVSDEESILLTGAKSYEQAAEEILAMGPKLVAITLGEHGVLMATKSR
KEIIKAFQTHAVDTTGAGDSFWGGVLCSILSINKPVEKMEWEEIRKCAVLGNAVAGLCVQKRGGIPAIPMKEAVFEFMQK

Specific function: Unknown

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Homo sapiens, GI11545855, Length=269, Percent_Identity=30.8550185873606, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI87081971, Length=329, Percent_Identity=23.7082066869301, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790193, Length=293, Percent_Identity=23.8907849829352, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082281, Length=308, Percent_Identity=20.7792207792208, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532905, Length=318, Percent_Identity=28.9308176100629, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.4

Molecular weight: Translated: 34955; Mature: 34955

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKVDIVALGELLIDFTEAGHSQGGRKLFEQNPGGAPANLLTVASHFGYRTSFIGKVGN
CCCCCHHEEHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHCCC
DMHGRFLKRTLQTEGINTDAIVEDPDYFTTLAFVEIGENGERNFSFARKPGADTQLKKEE
CHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
LDQTLISGCRIFHFGSLSLTDEPAESATIEAVKMAKAAGVLISYDPNYRPSLWKSKEYAV
HHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHH
KKMKSVVELVDVMKVSDEESILLTGAKSYEQAAEEILAMGPKLVAITLGEHGVLMATKSR
HHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECHH
KEIIKAFQTHAVDTTGAGDSFWGGVLCSILSINKPVEKMEWEEIRKCAVLGNAVAGLCVQ
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KRGGIPAIPMKEAVFEFMQK
CCCCCCCCCHHHHHHHHHHC
>Mature Secondary Structure
MEEKVDIVALGELLIDFTEAGHSQGGRKLFEQNPGGAPANLLTVASHFGYRTSFIGKVGN
CCCCCHHEEHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHCCC
DMHGRFLKRTLQTEGINTDAIVEDPDYFTTLAFVEIGENGERNFSFARKPGADTQLKKEE
CHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
LDQTLISGCRIFHFGSLSLTDEPAESATIEAVKMAKAAGVLISYDPNYRPSLWKSKEYAV
HHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHH
KKMKSVVELVDVMKVSDEESILLTGAKSYEQAAEEILAMGPKLVAITLGEHGVLMATKSR
HHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECHH
KEIIKAFQTHAVDTTGAGDSFWGGVLCSILSINKPVEKMEWEEIRKCAVLGNAVAGLCVQ
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KRGGIPAIPMKEAVFEFMQK
CCCCCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-fructose

Specific reaction: ATP + D-fructose = ADP + D-fructose 6-phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]