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The map label for this gene is hsdM [H]

Identifier: 238923778

GI number: 238923778

Start: 1280703

End: 1282337

Strand: Direct

Name: hsdM [H]

Synonym: EUBREC_1400

Alternate gene names: 238923778

Gene position: 1280703-1282337 (Clockwise)

Preceding gene: 238923777

Following gene: 238923779

Centisome position: 37.13

GC content: 36.39

Gene sequence:

>1635_bases
ATGCTTGGGTTGAAAGCGGACAAAGCGCAGAGGCTGACAAAAATAATACAGTGGAGGAAAAGCAAGATGGCAGTGGAAAA
TAAGAAAGAACAAGAAAGAGATGAACTTCATCGTGCGATATGGGCAATCGCTGATGAATTACGTGGTGCGGTAGATGGAT
GGGATTTCAAGAACTATGTGCTTGGAACAATGTTTTACAGATATATATCTGAAAATTTAACCGCGTATGTTAATGTAGGA
GAGATTGAAGCTGGAAATACAGATTTTGATTATGCAAAAATGAAAGATGCCGATGCAGAAGAAGCTCGTGAAGGGCTTGT
ACAGGAAAAAGGATTTTTTATTTTACCAAGTGAACTCTTCTGCAATGTTAGGGTTCGTGCAGCGGAAGATAATAATTTAA
ATGAGACACTTGAAAAAGTATTTAGACATATAGAGGAGTCTGCAAAGGGAAGTCAGTCAGAAAACAGCTTTGCTGGACTG
TTTGATGATTACGATGTAAACAGTAATAAGCTGGGATCAACAGTTGCAAAGAGAAACGAAAGACTGGTAAAGCTCCTTGA
TGGAATTGCATCTATGAATCTTGGATCAGTAAAAGATCATGATATAGATGCATTTGGTGATGCATATGAATATTTGATGA
CAATGTATGCTTCAAATGCTGGAAAATCAGGTGGAGAATTTTTTACACCTGCGGATGTTTCAGAGCTCTTAACTAGATTA
GGCACGGTTGGTAAAAAAACAGTAAATAAGGTATATGACCCTGCGTGTGGTTCGGGTTCCTTATTATTAAAAGCTGAAAA
GGTGTTAGGAAAAGATTCTGTCAAAATAGGATTCTTTGGGCAAGAGATAAATATCACAACATATAATCTTTGCCGTATTA
ATATGTTCCTTCACGATATTGGTTTTGATAAATTCGATATTGAGTGCGAGGACACCCTGACAAATCCACAGCATTGGGAT
GATGAGCCTTTCGAACTAATTGTTTCCAATCCCCCTTATTCCATTAAATGGGAAGGAGATGATAATCCATTACTTATAAA
TGATCCAAGATTTTCACCGGCTGGTGTTTTAGCACCTAAAAGTAAAGCCGATTTAGCATTTATCATGCACAGCCTTTCTT
GGTTAGCGGCAAATGGAACAGCTGCAATTGTGTGTTTCCCAGGAATTATGTACCGAGGTGGGGCAGAAAAGAAGATTCGA
CAATATCTAATTGATAATAACTTTATTGATTGTGTAATTCAGCTGCCAAGTAATCTGTTTTTTGGTACATCAATTGCAAC
GTGCATTATGGTAATGAAGAAAAATAAAACAGATAATAATACGTTATTTATCGATGCCTCTAATGAATGTGTAAAAGTAA
CAAACAATAATAAATTAACTAAAGAGAATATTGATCGTATTGTAGATGTATTTTCTAATAGAACCGAAGAAGAGCATCTT
TCATATTTGGCATCATATGAAGAAATTAAGGAGAAAGAATATAATCTTTCGGTTTCCACTTATGTAGAGGCAGAGGATAT
CAGAGAGAAAATTGACATTGTAAAGCTGAATGCGGAAATCAAAGAAATCGTGGCTCGTGAACAGGTTTTGAGGGATGAGA
TTGATAAAATCATCGCAGAAATCGAGGTGGATTAA

Upstream 100 bases:

>100_bases
CTAGGAGTCAGCAAAGATACAATCCGTGCATGGATAAGAAAAGAAACTATTCCTTTTTATAAAGTTGGGCGTCAGTATAA
ATTCAAGGTATCAGAAGTAG

Downstream 100 bases:

>100_bases
TAATGATACTTTATTTGAAAAGAGAAAAATCAGGGATTGATGCAATCATGAATTTTGATTCAAGTAATAGTTCATATACT
GTATTAAAAGGTTCTGTTTT

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MLGLKADKAQRLTKIIQWRKSKMAVENKKEQERDELHRAIWAIADELRGAVDGWDFKNYVLGTMFYRYISENLTAYVNVG
EIEAGNTDFDYAKMKDADAEEAREGLVQEKGFFILPSELFCNVRVRAAEDNNLNETLEKVFRHIEESAKGSQSENSFAGL
FDDYDVNSNKLGSTVAKRNERLVKLLDGIASMNLGSVKDHDIDAFGDAYEYLMTMYASNAGKSGGEFFTPADVSELLTRL
GTVGKKTVNKVYDPACGSGSLLLKAEKVLGKDSVKIGFFGQEINITTYNLCRINMFLHDIGFDKFDIECEDTLTNPQHWD
DEPFELIVSNPPYSIKWEGDDNPLLINDPRFSPAGVLAPKSKADLAFIMHSLSWLAANGTAAIVCFPGIMYRGGAEKKIR
QYLIDNNFIDCVIQLPSNLFFGTSIATCIMVMKKNKTDNNTLFIDASNECVKVTNNNKLTKENIDRIVDVFSNRTEEEHL
SYLASYEEIKEKEYNLSVSTYVEAEDIREKIDIVKLNAEIKEIVAREQVLRDEIDKIIAEIEVD

Sequences:

>Translated_544_residues
MLGLKADKAQRLTKIIQWRKSKMAVENKKEQERDELHRAIWAIADELRGAVDGWDFKNYVLGTMFYRYISENLTAYVNVG
EIEAGNTDFDYAKMKDADAEEAREGLVQEKGFFILPSELFCNVRVRAAEDNNLNETLEKVFRHIEESAKGSQSENSFAGL
FDDYDVNSNKLGSTVAKRNERLVKLLDGIASMNLGSVKDHDIDAFGDAYEYLMTMYASNAGKSGGEFFTPADVSELLTRL
GTVGKKTVNKVYDPACGSGSLLLKAEKVLGKDSVKIGFFGQEINITTYNLCRINMFLHDIGFDKFDIECEDTLTNPQHWD
DEPFELIVSNPPYSIKWEGDDNPLLINDPRFSPAGVLAPKSKADLAFIMHSLSWLAANGTAAIVCFPGIMYRGGAEKKIR
QYLIDNNFIDCVIQLPSNLFFGTSIATCIMVMKKNKTDNNTLFIDASNECVKVTNNNKLTKENIDRIVDVFSNRTEEEHL
SYLASYEEIKEKEYNLSVSTYVEAEDIREKIDIVKLNAEIKEIVAREQVLRDEIDKIIAEIEVD
>Mature_544_residues
MLGLKADKAQRLTKIIQWRKSKMAVENKKEQERDELHRAIWAIADELRGAVDGWDFKNYVLGTMFYRYISENLTAYVNVG
EIEAGNTDFDYAKMKDADAEEAREGLVQEKGFFILPSELFCNVRVRAAEDNNLNETLEKVFRHIEESAKGSQSENSFAGL
FDDYDVNSNKLGSTVAKRNERLVKLLDGIASMNLGSVKDHDIDAFGDAYEYLMTMYASNAGKSGGEFFTPADVSELLTRL
GTVGKKTVNKVYDPACGSGSLLLKAEKVLGKDSVKIGFFGQEINITTYNLCRINMFLHDIGFDKFDIECEDTLTNPQHWD
DEPFELIVSNPPYSIKWEGDDNPLLINDPRFSPAGVLAPKSKADLAFIMHSLSWLAANGTAAIVCFPGIMYRGGAEKKIR
QYLIDNNFIDCVIQLPSNLFFGTSIATCIMVMKKNKTDNNTLFIDASNECVKVTNNNKLTKENIDRIVDVFSNRTEEEHL
SYLASYEEIKEKEYNLSVSTYVEAEDIREKIDIVKLNAEIKEIVAREQVLRDEIDKIIAEIEVD

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=260, Percent_Identity=24.6153846153846, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 61399; Mature: 61399

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLKADKAQRLTKIIQWRKSKMAVENKKEQERDELHRAIWAIADELRGAVDGWDFKNYV
CCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LGTMFYRYISENLTAYVNVGEIEAGNTDFDYAKMKDADAEEAREGLVQEKGFFILPSELF
HHHHHHHHHHCCCEEEEEECEEECCCCCCCHHHCCCCCHHHHHHHHHHCCCCEEECHHHE
CNVRVRAAEDNNLNETLEKVFRHIEESAKGSQSENSFAGLFDDYDVNSNKLGSTVAKRNE
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RLVKLLDGIASMNLGSVKDHDIDAFGDAYEYLMTMYASNAGKSGGEFFTPADVSELLTRL
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
GTVGKKTVNKVYDPACGSGSLLLKAEKVLGKDSVKIGFFGQEINITTYNLCRINMFLHDI
HHCCHHHHHHHCCCCCCCCCEEEEEHHHCCCCCEEEEEECCEEEEEEEEEEEEEEHHHHC
GFDKFDIECEDTLTNPQHWDDEPFELIVSNPPYSIKWEGDDNPLLINDPRFSPAGVLAPK
CCCEEEEEEHHCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEECCCCCCCCCCCCC
SKADLAFIMHSLSWLAANGTAAIVCFPGIMYRGGAEKKIRQYLIDNNFIDCVIQLPSNLF
CCHHHHHHHHHHHHHCCCCCEEEEEECCHHCCCCCHHHHHHHHHCCCCEEEHEECCCCCE
FGTSIATCIMVMKKNKTDNNTLFIDASNECVKVTNNNKLTKENIDRIVDVFSNRTEEEHL
EHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHH
SYLASYEEIKEKEYNLSVSTYVEAEDIREKIDIVKLNAEIKEIVAREQVLRDEIDKIIAE
HHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHH
IEVD
HCCC
>Mature Secondary Structure
MLGLKADKAQRLTKIIQWRKSKMAVENKKEQERDELHRAIWAIADELRGAVDGWDFKNYV
CCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LGTMFYRYISENLTAYVNVGEIEAGNTDFDYAKMKDADAEEAREGLVQEKGFFILPSELF
HHHHHHHHHHCCCEEEEEECEEECCCCCCCHHHCCCCCHHHHHHHHHHCCCCEEECHHHE
CNVRVRAAEDNNLNETLEKVFRHIEESAKGSQSENSFAGLFDDYDVNSNKLGSTVAKRNE
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RLVKLLDGIASMNLGSVKDHDIDAFGDAYEYLMTMYASNAGKSGGEFFTPADVSELLTRL
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
GTVGKKTVNKVYDPACGSGSLLLKAEKVLGKDSVKIGFFGQEINITTYNLCRINMFLHDI
HHCCHHHHHHHCCCCCCCCCEEEEEHHHCCCCCEEEEEECCEEEEEEEEEEEEEEHHHHC
GFDKFDIECEDTLTNPQHWDDEPFELIVSNPPYSIKWEGDDNPLLINDPRFSPAGVLAPK
CCCEEEEEEHHCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEECCCCCCCCCCCCC
SKADLAFIMHSLSWLAANGTAAIVCFPGIMYRGGAEKKIRQYLIDNNFIDCVIQLPSNLF
CCHHHHHHHHHHHHHCCCCCEEEEEECCHHCCCCCHHHHHHHHHCCCCEEEHEECCCCCE
FGTSIATCIMVMKKNKTDNNTLFIDASNECVKVTNNNKLTKENIDRIVDVFSNRTEEEHL
EHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHH
SYLASYEEIKEKEYNLSVSTYVEAEDIREKIDIVKLNAEIKEIVAREQVLRDEIDKIIAE
HHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHH
IEVD
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]