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The map label for this gene is lacF [H]

Identifier: 238923761

GI number: 238923761

Start: 1257892

End: 1258794

Strand: Direct

Name: lacF [H]

Synonym: EUBREC_1383

Alternate gene names: 238923761

Gene position: 1257892-1258794 (Clockwise)

Preceding gene: 238923760

Following gene: 238923762

Centisome position: 36.46

GC content: 38.87

Gene sequence:

>903_bases
GTGAAAAATAAGAAAAAAGGAATGAGCATAACCGGTAAACAAAGGGCAGCCGGCTGGATGTATCTGGCTCCGGCAACAAT
ACTCATTTTCATAATGAGCTTCTGGCCTATAATCCAGGCTGTTATTACATCATTTAAAACCGGTTCAAGTGCAAATATGC
AGTGGGCTAATCCATTTGCCTACAATTACACAAGAATGTTTCAGGATGCGGTTTTCAAGAGATCTATCGGAAACACATTC
CTATATTTAATTATCGAAGTTCCAATCATGCTTGTACTGGCAATTTTACTGGCACAGTTACTTAACAACAAGCATCTTAA
ATTTAAAGGACTTTTCCGTACATGTGTATTTTTACCATGTGCAACATCACTCGTATCATATGCATTGATTTTTAAATCGC
TTTTTGCAACACAGGGACTTATCAATACAATCCTTGTTAAACTTGGAATATTAGAGAATAACTTTAACTTCCTTGGTACA
GGCTGGAGTGCTAAGATTATTATCATAGTAGCTCTTATCTGGAGATGGACAGGATATAATATGGTGTTCTTTTTAGCAGG
TCTCCAGAATATTGAGTATTCTGTTTACGAGGCGGCAAAGATAGATGGAGCAAGCGGCTGGAGAACCTTCTGGTCTATCA
CGGTTCCTCTTCTTAGACCTACAATTGTTATGACAACCATCATGTCAATAAACGGTACATTACAGCTCTTCGACGAGTCT
GTAAACCTGACAAAGGGTGGACCTGCAAATGCGACAATCACCATGTCACATTATATCTATAACGGCTCGTTTGGTGAAGG
CGTTGCAAACTTTGGATATGCATCAGCAATGTCTGTTATCGTATTCATCATGGTTGCTATACTGGCATTTATCAATCTGA
AAGTAGGTGACAAGCGTGACTAA

Upstream 100 bases:

>100_bases
CAATGGGACTTTAATCTGTTAGCTTGATGGCAAATGTACAGGGAGCCATTAAATGGCTCCCTGTTTTTTCTCAAAGACTT
ATAGTTAGGAGGCCTTTACA

Downstream 100 bases:

>100_bases
GAAAAAACATGGATCATCAATACAAAAAAGGCTTATACCTTCATACATTTTTCTTGTAATAGTTTCGTTCATCTCAGTAT
TTCCGTTTTTCTGGATGATA

Product: lactose transport system (permease)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKNKKKGMSITGKQRAAGWMYLAPATILIFIMSFWPIIQAVITSFKTGSSANMQWANPFAYNYTRMFQDAVFKRSIGNTF
LYLIIEVPIMLVLAILLAQLLNNKHLKFKGLFRTCVFLPCATSLVSYALIFKSLFATQGLINTILVKLGILENNFNFLGT
GWSAKIIIIVALIWRWTGYNMVFFLAGLQNIEYSVYEAAKIDGASGWRTFWSITVPLLRPTIVMTTIMSINGTLQLFDES
VNLTKGGPANATITMSHYIYNGSFGEGVANFGYASAMSVIVFIMVAILAFINLKVGDKRD

Sequences:

>Translated_300_residues
MKNKKKGMSITGKQRAAGWMYLAPATILIFIMSFWPIIQAVITSFKTGSSANMQWANPFAYNYTRMFQDAVFKRSIGNTF
LYLIIEVPIMLVLAILLAQLLNNKHLKFKGLFRTCVFLPCATSLVSYALIFKSLFATQGLINTILVKLGILENNFNFLGT
GWSAKIIIIVALIWRWTGYNMVFFLAGLQNIEYSVYEAAKIDGASGWRTFWSITVPLLRPTIVMTTIMSINGTLQLFDES
VNLTKGGPANATITMSHYIYNGSFGEGVANFGYASAMSVIVFIMVAILAFINLKVGDKRD
>Mature_300_residues
MKNKKKGMSITGKQRAAGWMYLAPATILIFIMSFWPIIQAVITSFKTGSSANMQWANPFAYNYTRMFQDAVFKRSIGNTF
LYLIIEVPIMLVLAILLAQLLNNKHLKFKGLFRTCVFLPCATSLVSYALIFKSLFATQGLINTILVKLGILENNFNFLGT
GWSAKIIIIVALIWRWTGYNMVFFLAGLQNIEYSVYEAAKIDGASGWRTFWSITVPLLRPTIVMTTIMSINGTLQLFDES
VNLTKGGPANATITMSHYIYNGSFGEGVANFGYASAMSVIVFIMVAILAFINLKVGDKRD

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=301, Percent_Identity=25.9136212624585, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790465, Length=248, Percent_Identity=30.6451612903226, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787570, Length=254, Percent_Identity=26.3779527559055, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33454; Mature: 33454

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKKKGMSITGKQRAAGWMYLAPATILIFIMSFWPIIQAVITSFKTGSSANMQWANPFA
CCCCCCCCCCCCCHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCH
YNYTRMFQDAVFKRSIGNTFLYLIIEVPIMLVLAILLAQLLNNKHLKFKGLFRTCVFLPC
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ATSLVSYALIFKSLFATQGLINTILVKLGILENNFNFLGTGWSAKIIIIVALIWRWTGYN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCHHHHHHHHHHHHHCCCC
MVFFLAGLQNIEYSVYEAAKIDGASGWRTFWSITVPLLRPTIVMTTIMSINGTLQLFDES
HHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHEEEHHHHHHHHHHHHHHHCCCEEEEEECC
VNLTKGGPANATITMSHYIYNGSFGEGVANFGYASAMSVIVFIMVAILAFINLKVGDKRD
CCCCCCCCCCEEEEEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKNKKKGMSITGKQRAAGWMYLAPATILIFIMSFWPIIQAVITSFKTGSSANMQWANPFA
CCCCCCCCCCCCCHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCH
YNYTRMFQDAVFKRSIGNTFLYLIIEVPIMLVLAILLAQLLNNKHLKFKGLFRTCVFLPC
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ATSLVSYALIFKSLFATQGLINTILVKLGILENNFNFLGTGWSAKIIIIVALIWRWTGYN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCHHHHHHHHHHHHHCCCC
MVFFLAGLQNIEYSVYEAAKIDGASGWRTFWSITVPLLRPTIVMTTIMSINGTLQLFDES
HHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHEEEHHHHHHHHHHHHHHHCCCEEEEEECC
VNLTKGGPANATITMSHYIYNGSFGEGVANFGYASAMSVIVFIMVAILAFINLKVGDKRD
CCCCCCCCCCEEEEEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]