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The map label for this gene is phoB [H]

Identifier: 238923747

GI number: 238923747

Start: 1243973

End: 1244644

Strand: Direct

Name: phoB [H]

Synonym: EUBREC_1369

Alternate gene names: 238923747

Gene position: 1243973-1244644 (Clockwise)

Preceding gene: 238923746

Following gene: 238923748

Centisome position: 36.06

GC content: 44.2

Gene sequence:

>672_bases
ATGAAAAAAATACTTCTCATAGAGGACAATAAAGGAATAACAGAGGGACTGTGTGATACCTTTACAGAACCTGAATATGA
GCTTTTGACAGCAGGCAATTTAAAGGAGGCTGGGGCGCTTTTGAGTGAGCACCCCGGCCTTGTGCTGCTTGATGTGTCGC
TTCCGGATGGGAACGGCTTTGATTTTTACAGCGAAAGGCTGGAAAAAGAAAAAACACCGGTGATTTTTCTTACCGCAAGG
GATGAGGAAAATGATATAGTAAAGGGACTTGAGCTTGGAGCGGAGGATTACATCACAAAGCCTTTTTCCGTAAGGGAGCT
TGTGGCGAGGGTAAACCGCGTATTTTTACGTAGGAAAAGTGATGACAGTTCATCTGTCATACATGCAGGAAGCATAAGCT
ATGATTTGGACAAAAAGGAAGCAAGAAGGGATGGACAGTTGATTGCTCTTTCAAGTCTTGAAAACCGCATACTTGATCTG
CTTTTTACAAATCACGACAAGGCTGTGTCCAGAAATGCCGTGCTTGACTGTATCTGGGAGGCGACAGGCAACGATGTCTA
CGACCATACAGTGACTGTCTATATGAAGCGCATCAGGGCAAAGCTTGGTGAGAATGTGATAAAGACTGTGAAGGGAATCG
GGTATCGTGTTGATTCAGATGAAGCTCTTTAA

Upstream 100 bases:

>100_bases
TGATTTATGCTATCATGAAACTATCAATGATGAAGCTTAGACACAATAACATTATTGAGACAATCAAAAATGAAAATCTG
TAGGAAATGCGGGAGCTGAT

Downstream 100 bases:

>100_bases
ACAGTATCGGTCGACAGAGACAGTTCCCGGGGGAAAGCATGAGAAAGAAGAAATATAATTCAGAGAACAGAAAACATTTT
GTGTATTTTGCAATAATATA

Product: phosphate regulon transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKKILLIEDNKGITEGLCDTFTEPEYELLTAGNLKEAGALLSEHPGLVLLDVSLPDGNGFDFYSERLEKEKTPVIFLTAR
DEENDIVKGLELGAEDYITKPFSVRELVARVNRVFLRRKSDDSSSVIHAGSISYDLDKKEARRDGQLIALSSLENRILDL
LFTNHDKAVSRNAVLDCIWEATGNDVYDHTVTVYMKRIRAKLGENVIKTVKGIGYRVDSDEAL

Sequences:

>Translated_223_residues
MKKILLIEDNKGITEGLCDTFTEPEYELLTAGNLKEAGALLSEHPGLVLLDVSLPDGNGFDFYSERLEKEKTPVIFLTAR
DEENDIVKGLELGAEDYITKPFSVRELVARVNRVFLRRKSDDSSSVIHAGSISYDLDKKEARRDGQLIALSSLENRILDL
LFTNHDKAVSRNAVLDCIWEATGNDVYDHTVTVYMKRIRAKLGENVIKTVKGIGYRVDSDEAL
>Mature_223_residues
MKKILLIEDNKGITEGLCDTFTEPEYELLTAGNLKEAGALLSEHPGLVLLDVSLPDGNGFDFYSERLEKEKTPVIFLTAR
DEENDIVKGLELGAEDYITKPFSVRELVARVNRVFLRRKSDDSSSVIHAGSISYDLDKKEARRDGQLIALSSLENRILDL
LFTNHDKAVSRNAVLDCIWEATGNDVYDHTVTVYMKRIRAKLGENVIKTVKGIGYRVDSDEAL

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=34.9344978165939, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1786911, Length=228, Percent_Identity=32.4561403508772, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790860, Length=222, Percent_Identity=35.5855855855856, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1790552, Length=222, Percent_Identity=31.981981981982, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1787375, Length=219, Percent_Identity=31.9634703196347, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789402, Length=216, Percent_Identity=34.2592592592593, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87082012, Length=218, Percent_Identity=34.8623853211009, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=31.0810810810811, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI2367329, Length=230, Percent_Identity=34.7826086956522, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=31.4159292035398, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=30.5676855895196, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787229, Length=224, Percent_Identity=29.4642857142857, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790863, Length=233, Percent_Identity=32.618025751073, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788550, Length=117, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693140, Length=192, Percent_Identity=29.1666666666667, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 24992; Mature: 24992

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILLIEDNKGITEGLCDTFTEPEYELLTAGNLKEAGALLSEHPGLVLLDVSLPDGNGF
CCEEEEEECCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCC
DFYSERLEKEKTPVIFLTARDEENDIVKGLELGAEDYITKPFSVRELVARVNRVFLRRKS
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCC
DDSSSVIHAGSISYDLDKKEARRDGQLIALSSLENRILDLLFTNHDKAVSRNAVLDCIWE
CCCCCEEEECCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ATGNDVYDHTVTVYMKRIRAKLGENVIKTVKGIGYRVDSDEAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCC
>Mature Secondary Structure
MKKILLIEDNKGITEGLCDTFTEPEYELLTAGNLKEAGALLSEHPGLVLLDVSLPDGNGF
CCEEEEEECCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCC
DFYSERLEKEKTPVIFLTARDEENDIVKGLELGAEDYITKPFSVRELVARVNRVFLRRKS
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCC
DDSSSVIHAGSISYDLDKKEARRDGQLIALSSLENRILDLLFTNHDKAVSRNAVLDCIWE
CCCCCEEEECCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ATGNDVYDHTVTVYMKRIRAKLGENVIKTVKGIGYRVDSDEAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]