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The map label for this gene is leuA [C]

Identifier: 238923698

GI number: 238923698

Start: 1197304

End: 1198275

Strand: Direct

Name: leuA [C]

Synonym: EUBREC_1320

Alternate gene names: 238923698

Gene position: 1197304-1198275 (Clockwise)

Preceding gene: 238923696

Following gene: 238923699

Centisome position: 34.71

GC content: 45.68

Gene sequence:

>972_bases
ATGGCTCAGAAAGGTGATTTAATGACAGCACGCCCTGATATCCGCGTGCTTGACGCTACCATTAGGGATGGAGGACTGGT
AAACAACTTTATGTTTACAGATGATTTTATCAATGCTCTTTATCGTGCAAACGTAAAGGCAGGCATCGACTATATGGAGT
TTGGTTATAAAGCATCTAAGGAGCTTTTTGATGTAAATAAATTCGGCAAATGGAAGTTCTGTGATGAGGCAGCTATCCGT
GAGATAGTCGGAGACAACAACTCTGATATGAAGATTTCTGTCATGGCGGATGTCGGCAGATGTGACTACAAGAAGGATAT
TCTCCCTCGCGAGGACAGTGTGATTGACATGGTGCGTGTGGCTACATATGTACATCAGATGCCAGCAGCAATCGAGATGA
TTGAGGATGCCAAGTCCAAGGGCTACGAGGTAACCTGCAACATCATGGCCATCTCAAATACACAGGAATCAGACCTTGAT
CAGGCACTTGACATGGTTGCCAAGAGCTCTGCAGACGGCATATACATCGTAGACAGCTACGGAGCTCTCTACCCTGAGCA
GATTCGCCGCATTGCCGAGAAATACACAGAAATTGCTGAGGCAAACGGAAAGTTCGTAGGAATGCATGCGCACAATAATC
AGCAGCTTGCATTTGCAAACACCATAGAGGCAGCAGCCCAGGGAGTCAGCCTGTTGGATGCAACAATGATGGGCATGGGA
CGAGGCGCAGGAAACTGTGCGATGGAGCTTTTACTCAGCTTCCTAAAAAATCCAAAATACAATGTATTCCCTGTACTTAA
ATTTATTGAAGAGCATATGCTTCCGCTTAAGGAGAGCGGTGCTGTATGGGGCTACGATATTCCATACCTGCTCACAGGAC
GCTTAAATCAGCATCCATCAGCAGCCATTGACTACATCAAGTCAGGCGAGACACATTACGCTGATTTCTACACAGATTTG
CTTGATAAATAA

Upstream 100 bases:

>100_bases
TGCTTTAAAGCGATGAAGCATTAAAACTTTAAATTGTAACATCAAGCTACAGTAATATTTATATTTGTTACAATAATATT
TATACTTGGAGGAAACAAAA

Downstream 100 bases:

>100_bases
AAATAGATAGATGTATATAAATCAATCCGCTATGCCAGATTTTGGTGTAGCGGATTGTTTTTTATATGTTATAGTAATTT
TTTGTTGGTGATAAATCATA

Product: HMGL family protein

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MAQKGDLMTARPDIRVLDATIRDGGLVNNFMFTDDFINALYRANVKAGIDYMEFGYKASKELFDVNKFGKWKFCDEAAIR
EIVGDNNSDMKISVMADVGRCDYKKDILPREDSVIDMVRVATYVHQMPAAIEMIEDAKSKGYEVTCNIMAISNTQESDLD
QALDMVAKSSADGIYIVDSYGALYPEQIRRIAEKYTEIAEANGKFVGMHAHNNQQLAFANTIEAAAQGVSLLDATMMGMG
RGAGNCAMELLLSFLKNPKYNVFPVLKFIEEHMLPLKESGAVWGYDIPYLLTGRLNQHPSAAIDYIKSGETHYADFYTDL
LDK

Sequences:

>Translated_323_residues
MAQKGDLMTARPDIRVLDATIRDGGLVNNFMFTDDFINALYRANVKAGIDYMEFGYKASKELFDVNKFGKWKFCDEAAIR
EIVGDNNSDMKISVMADVGRCDYKKDILPREDSVIDMVRVATYVHQMPAAIEMIEDAKSKGYEVTCNIMAISNTQESDLD
QALDMVAKSSADGIYIVDSYGALYPEQIRRIAEKYTEIAEANGKFVGMHAHNNQQLAFANTIEAAAQGVSLLDATMMGMG
RGAGNCAMELLLSFLKNPKYNVFPVLKFIEEHMLPLKESGAVWGYDIPYLLTGRLNQHPSAAIDYIKSGETHYADFYTDL
LDK
>Mature_322_residues
AQKGDLMTARPDIRVLDATIRDGGLVNNFMFTDDFINALYRANVKAGIDYMEFGYKASKELFDVNKFGKWKFCDEAAIRE
IVGDNNSDMKISVMADVGRCDYKKDILPREDSVIDMVRVATYVHQMPAAIEMIEDAKSKGYEVTCNIMAISNTQESDLDQ
ALDMVAKSSADGIYIVDSYGALYPEQIRRIAEKYTEIAEANGKFVGMHAHNNQQLAFANTIEAAAQGVSLLDATMMGMGR
GAGNCAMELLLSFLKNPKYNVFPVLKFIEEHMLPLKESGAVWGYDIPYLLTGRLNQHPSAAIDYIKSGETHYADFYTDLL
DK

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family [H]

Homologues:

None

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- ProDom:   PD005364 [H]

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 36054; Mature: 35922

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKGDLMTARPDIRVLDATIRDGGLVNNFMFTDDFINALYRANVKAGIDYMEFGYKASK
CCCCCCCEECCCCCEEEEEECCCCCCEECCEEHHHHHHHHHHCCHHHCCHHHHHCCHHHH
ELFDVNKFGKWKFCDEAAIREIVGDNNSDMKISVMADVGRCDYKKDILPREDSVIDMVRV
HHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHCCCCCCCHHHHHHHH
ATYVHQMPAAIEMIEDAKSKGYEVTCNIMAISNTQESDLDQALDMVAKSSADGIYIVDSY
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECC
GALYPEQIRRIAEKYTEIAEANGKFVGMHAHNNQQLAFANTIEAAAQGVSLLDATMMGMG
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCC
RGAGNCAMELLLSFLKNPKYNVFPVLKFIEEHMLPLKESGAVWGYDIPYLLTGRLNQHPS
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHCCCEECCCCCEEEECCCCCCCH
AAIDYIKSGETHYADFYTDLLDK
HHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AQKGDLMTARPDIRVLDATIRDGGLVNNFMFTDDFINALYRANVKAGIDYMEFGYKASK
CCCCCCEECCCCCEEEEEECCCCCCEECCEEHHHHHHHHHHCCHHHCCHHHHHCCHHHH
ELFDVNKFGKWKFCDEAAIREIVGDNNSDMKISVMADVGRCDYKKDILPREDSVIDMVRV
HHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHCCCCCCCHHHHHHHH
ATYVHQMPAAIEMIEDAKSKGYEVTCNIMAISNTQESDLDQALDMVAKSSADGIYIVDSY
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECC
GALYPEQIRRIAEKYTEIAEANGKFVGMHAHNNQQLAFANTIEAAAQGVSLLDATMMGMG
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCC
RGAGNCAMELLLSFLKNPKYNVFPVLKFIEEHMLPLKESGAVWGYDIPYLLTGRLNQHPS
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHCCCEECCCCCEEEECCCCCCCH
AAIDYIKSGETHYADFYTDLLDK
HHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA