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The map label for this gene is mepA [H]

Identifier: 238923616

GI number: 238923616

Start: 1136250

End: 1137617

Strand: Direct

Name: mepA [H]

Synonym: EUBREC_1238

Alternate gene names: 238923616

Gene position: 1136250-1137617 (Clockwise)

Preceding gene: 238923615

Following gene: 238923617

Centisome position: 32.94

GC content: 38.38

Gene sequence:

>1368_bases
ATGGCAGAAAGTAACAAGATGAAGGACATGCCGGTAAATAAGCTCATGGTACAGATGGGAATACCGATGATTTTATCTAT
GGCATTGCAGGCAGTTTACAATATTGTAGACAGCGCCTTTGTGGGAAATATGAGGTCAGGAAGTGAGGCAGCACTTAATG
CACTGACACTGGTATTTCCGGTTCAGATGCTTATGGTAGCAGTTGGAATTGGAACAGGAGTGGGAACCAATGCACTTCTT
GCAAGAACACTTGGTCAGGGAAATAGTAAAAAAGCTGCAAAGGTTGCAGGAAACAGTTTATTTCTTGGTGCAATAATTTA
TATAGTATGCCTGTTGTTTGGAATTTTTGGAGTTAAAGCTTATATTTCATCACAGACCGTTGATCCTCAAGTTATTTCAA
TGGGAACAAATTACTTGAGAATATGTTGTGTGATTTCATTTGGAATTATTTTCTTTTCACTATTTGAAAAACTGCTGCAG
GCAACAGGACGTTCTCTTTATTCTACAATTGGTCAGGTAGTTGGAGCAGTGGTAAATATTATTCTTGATCCTATTATGAT
TTATGGTATTGGACCGGTTCCGGAAATGGGAGTTAAGGGCGCCGCATATGCAACTGTTATCGGTCAGGTTGTGTCTGCAA
TACTGCTGTTTGTGTTTCATATGAAGCTGAACAGGGAATTTGAGCATGATACAAAGTATATGAAGCCTGATGGAGGAATA
ATCAGGGAAATTTATGCAATTGGACTTCCGGCTATTATTGCACAGGCTCTGATGTCAATTATGGTTTATGTGATGAATCT
TATTTTGAAATTTAGTCCTTCGGCGCAGACTGCATATGGTTTGTTTTATAAGGTGCAGCAGTTTGTATTGTTCCTTGCAT
TCGGACTAAGGGATGCAATTACACCAATTATAGCTTTTTCATATGGAATGCATAGCAAAAAGAGAATTAAGGATGGCATC
AGGTATGGATTGCTTTATACGATTGTGCTTATGGTCATAGGAGTAGCGATTACAGAAATTTTCCCGGGAGAATTTGCTGC
TTTGTTCAATGCAGGAGCATCAAGAGAATATTTTATCGGGGCAATGAGAATTATTTCCATCAGCTTTATTTTTGCCGGAA
TAAATGTGGCATATCAGGGTATTTACCAGGCGCTTGATGGTGGTATGGAATCGCTTGTGATTTCACTGCTCAGACAGCTT
ATAATTATATTACCTTTGGCATGCATCTTCTCATTTTTTGTAAGAGGCGGACATATTGGAGTTTCGCTGATCTGGTGGGC
ATTTCCAATCACGGAGGTGACTGCTTGTATTGTAGGATATTTATTTTTAAAGAGAATCAATAAAAATAAAGTGGAAAACT
TAAATTAA

Upstream 100 bases:

>100_bases
AATATATAGTGCATTATAAAGTGTGTAGCTTATCAGCGTAAGTCCAGCTTATGAGATAAGAGCGCACTTTTTTTATGCAG
AAAAGGAGGAATAAGTTAAG

Downstream 100 bases:

>100_bases
GGAGGCATAAGCCATGACAAAAAGAATCATTACGATAAGCCGAGAGTTTGGAAGTGGCGGACGTTTTATCGGAGAAGAAG
TCGCAAATAAACTTGGAATT

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MAESNKMKDMPVNKLMVQMGIPMILSMALQAVYNIVDSAFVGNMRSGSEAALNALTLVFPVQMLMVAVGIGTGVGTNALL
ARTLGQGNSKKAAKVAGNSLFLGAIIYIVCLLFGIFGVKAYISSQTVDPQVISMGTNYLRICCVISFGIIFFSLFEKLLQ
ATGRSLYSTIGQVVGAVVNIILDPIMIYGIGPVPEMGVKGAAYATVIGQVVSAILLFVFHMKLNREFEHDTKYMKPDGGI
IREIYAIGLPAIIAQALMSIMVYVMNLILKFSPSAQTAYGLFYKVQQFVLFLAFGLRDAITPIIAFSYGMHSKKRIKDGI
RYGLLYTIVLMVIGVAITEIFPGEFAALFNAGASREYFIGAMRIISISFIFAGINVAYQGIYQALDGGMESLVISLLRQL
IIILPLACIFSFFVRGGHIGVSLIWWAFPITEVTACIVGYLFLKRINKNKVENLN

Sequences:

>Translated_455_residues
MAESNKMKDMPVNKLMVQMGIPMILSMALQAVYNIVDSAFVGNMRSGSEAALNALTLVFPVQMLMVAVGIGTGVGTNALL
ARTLGQGNSKKAAKVAGNSLFLGAIIYIVCLLFGIFGVKAYISSQTVDPQVISMGTNYLRICCVISFGIIFFSLFEKLLQ
ATGRSLYSTIGQVVGAVVNIILDPIMIYGIGPVPEMGVKGAAYATVIGQVVSAILLFVFHMKLNREFEHDTKYMKPDGGI
IREIYAIGLPAIIAQALMSIMVYVMNLILKFSPSAQTAYGLFYKVQQFVLFLAFGLRDAITPIIAFSYGMHSKKRIKDGI
RYGLLYTIVLMVIGVAITEIFPGEFAALFNAGASREYFIGAMRIISISFIFAGINVAYQGIYQALDGGMESLVISLLRQL
IIILPLACIFSFFVRGGHIGVSLIWWAFPITEVTACIVGYLFLKRINKNKVENLN
>Mature_454_residues
AESNKMKDMPVNKLMVQMGIPMILSMALQAVYNIVDSAFVGNMRSGSEAALNALTLVFPVQMLMVAVGIGTGVGTNALLA
RTLGQGNSKKAAKVAGNSLFLGAIIYIVCLLFGIFGVKAYISSQTVDPQVISMGTNYLRICCVISFGIIFFSLFEKLLQA
TGRSLYSTIGQVVGAVVNIILDPIMIYGIGPVPEMGVKGAAYATVIGQVVSAILLFVFHMKLNREFEHDTKYMKPDGGII
REIYAIGLPAIIAQALMSIMVYVMNLILKFSPSAQTAYGLFYKVQQFVLFLAFGLRDAITPIIAFSYGMHSKKRIKDGIR
YGLLYTIVLMVIGVAITEIFPGEFAALFNAGASREYFIGAMRIISISFIFAGINVAYQGIYQALDGGMESLVISLLRQLI
IILPLACIFSFFVRGGHIGVSLIWWAFPITEVTACIVGYLFLKRINKNKVENLN

Specific function: Multidrug resistance efflux protein [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MepA subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994890, Length=344, Percent_Identity=21.5116279069767, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR015522 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49553; Mature: 49422

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESNKMKDMPVNKLMVQMGIPMILSMALQAVYNIVDSAFVGNMRSGSEAALNALTLVFP
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VQMLMVAVGIGTGVGTNALLARTLGQGNSKKAAKVAGNSLFLGAIIYIVCLLFGIFGVKA
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
YISSQTVDPQVISMGTNYLRICCVISFGIIFFSLFEKLLQATGRSLYSTIGQVVGAVVNI
HHHCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ILDPIMIYGIGPVPEMGVKGAAYATVIGQVVSAILLFVFHMKLNREFEHDTKYMKPDGGI
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHH
IREIYAIGLPAIIAQALMSIMVYVMNLILKFSPSAQTAYGLFYKVQQFVLFLAFGLRDAI
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TPIIAFSYGMHSKKRIKDGIRYGLLYTIVLMVIGVAITEIFPGEFAALFNAGASREYFIG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHH
AMRIISISFIFAGINVAYQGIYQALDGGMESLVISLLRQLIIILPLACIFSFFVRGGHIG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VSLIWWAFPITEVTACIVGYLFLKRINKNKVENLN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AESNKMKDMPVNKLMVQMGIPMILSMALQAVYNIVDSAFVGNMRSGSEAALNALTLVFP
CCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA