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The map label for this gene is glyA

Identifier: 238923499

GI number: 238923499

Start: 1032378

End: 1033619

Strand: Direct

Name: glyA

Synonym: EUBREC_1119

Alternate gene names: 238923499

Gene position: 1032378-1033619 (Clockwise)

Preceding gene: 238923496

Following gene: 238923500

Centisome position: 29.93

GC content: 48.71

Gene sequence:

>1242_bases
ATGTATACACTTGACGACATCAAAAAAGAAGATCCTGAGATTGCGAGCGCCATCACAGATGAGTTTGAGCGCCAGAACAG
CCACATCGAGCTTATTGCTTCTGAGAACTGGGTAAGCCCTGCTGTTATGTCAGCTATGGGAAGCATCCTCACCAATAAGT
ACGCTGAGGGATATCCAGGAAGAAGATACTATGGCGGCTGTGAGTGCGTGGACGAGGTAGAGGAGCTTGCAAGAGAAAGA
GCCAAGGAGCTTTTCGGCGCAGAGTATGTAAATGTACAGCCACACTCAGGTGCTCAGGCCAACATGGCAGTACAGTTCGC
CATCTTAAAGCCGGGCGATACCATCATGGGAATGAATCTTGACCACGGCGGACACCTCACACATGGTTCACCGGTCAACT
TCTCAGGCTCATATTTCCATGTAGTGCCTTATGGAGTAAATGACGAGGGCTTCATCGATTACGACAAGGTAGAGGAGATC
GCCCTTGAGTGCAAGCCAAAGATGATTATCGCAGGTGCATCTGCATATGCGAGAACTATAGATTTCAAGCGTTTCAGAGA
GATTGCAGACAAGGTAGACGCAGTGCTCATGGTTGATATGGCTCATATCGCAGGACTTGTAGCCGCAGGACTTCATCCAA
GCCCAATCCCATATGCACATGTCACCACAACCACTACACACAAGACTCTCCGTGGACCTCGTGGAGGAATGATCCTCTGC
AGCCAGGAGATGCAGGATAAGTACAATTTCAACAAGGCTATTTTCCCGGGAATCCAGGGTGGACCTCTCATGCACGTTAT
CGCTGCAAAGGCAGTCTGCTTCAAGGAGGCATTACAGCCTGAGTTCAAGGAGTATCAGAAGCAGATTGTAAAGAACGCAC
AGGCACTTTGCAAGGGTCTTCAGAGCCGTGGCATCAAGATTGTATCAGACGGCACAGACAACCACCTCATGCTCGTAGAT
TTAACTCCGTTTGGTCTCACAGGAAAATCTATCGAGAAGCTTCTCGATGCGGCTCATATCACAGCCAACAAGAATACTAT
TCCTAACGACCCACAGAAGCCATTTGTCACAAGCGGTATCCGTCTCGGAACACCTGCTGCTACATCAAGAGGCTTAAAGG
AGGATGATTTCGACAAGGTTGCAGAGGCAATCGCCATGATCATCAAAGAGGGCGAGAGCGCAGTTGAGCCTGCAAAGGCT
ATCATCAAGACACTTACTGACAAGTATCCTCTTGCATTTTAA

Upstream 100 bases:

>100_bases
ATTTTTGCGCGGTAGCGCATCAAAGTAATAAATCACAGCAAAGCATATAATTGCGATAAATAATGCAAGTATTTTGCAAT
AAAGAAAAGGAGACACATAT

Downstream 100 bases:

>100_bases
GGGTTTTCAGAGTAAAATGTTATAAGTTATAAAAACATAATACTTTGGAGGAATTATGATAGACTTAAAGTTTTTAAGAG
AGAATCCGGATCTCGTAAAA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MYTLDDIKKEDPEIASAITDEFERQNSHIELIASENWVSPAVMSAMGSILTNKYAEGYPGRRYYGGCECVDEVEELARER
AKELFGAEYVNVQPHSGAQANMAVQFAILKPGDTIMGMNLDHGGHLTHGSPVNFSGSYFHVVPYGVNDEGFIDYDKVEEI
ALECKPKMIIAGASAYARTIDFKRFREIADKVDAVLMVDMAHIAGLVAAGLHPSPIPYAHVTTTTTHKTLRGPRGGMILC
SQEMQDKYNFNKAIFPGIQGGPLMHVIAAKAVCFKEALQPEFKEYQKQIVKNAQALCKGLQSRGIKIVSDGTDNHLMLVD
LTPFGLTGKSIEKLLDAAHITANKNTIPNDPQKPFVTSGIRLGTPAATSRGLKEDDFDKVAEAIAMIIKEGESAVEPAKA
IIKTLTDKYPLAF

Sequences:

>Translated_413_residues
MYTLDDIKKEDPEIASAITDEFERQNSHIELIASENWVSPAVMSAMGSILTNKYAEGYPGRRYYGGCECVDEVEELARER
AKELFGAEYVNVQPHSGAQANMAVQFAILKPGDTIMGMNLDHGGHLTHGSPVNFSGSYFHVVPYGVNDEGFIDYDKVEEI
ALECKPKMIIAGASAYARTIDFKRFREIADKVDAVLMVDMAHIAGLVAAGLHPSPIPYAHVTTTTTHKTLRGPRGGMILC
SQEMQDKYNFNKAIFPGIQGGPLMHVIAAKAVCFKEALQPEFKEYQKQIVKNAQALCKGLQSRGIKIVSDGTDNHLMLVD
LTPFGLTGKSIEKLLDAAHITANKNTIPNDPQKPFVTSGIRLGTPAATSRGLKEDDFDKVAEAIAMIIKEGESAVEPAKA
IIKTLTDKYPLAF
>Mature_413_residues
MYTLDDIKKEDPEIASAITDEFERQNSHIELIASENWVSPAVMSAMGSILTNKYAEGYPGRRYYGGCECVDEVEELARER
AKELFGAEYVNVQPHSGAQANMAVQFAILKPGDTIMGMNLDHGGHLTHGSPVNFSGSYFHVVPYGVNDEGFIDYDKVEEI
ALECKPKMIIAGASAYARTIDFKRFREIADKVDAVLMVDMAHIAGLVAAGLHPSPIPYAHVTTTTTHKTLRGPRGGMILC
SQEMQDKYNFNKAIFPGIQGGPLMHVIAAKAVCFKEALQPEFKEYQKQIVKNAQALCKGLQSRGIKIVSDGTDNHLMLVD
LTPFGLTGKSIEKLLDAAHITANKNTIPNDPQKPFVTSGIRLGTPAATSRGLKEDDFDKVAEAIAMIIKEGESAVEPAKA
IIKTLTDKYPLAF

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=405, Percent_Identity=48.3950617283951, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI261862352, Length=405, Percent_Identity=48.3950617283951, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI261862350, Length=405, Percent_Identity=48.3950617283951, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI261862348, Length=405, Percent_Identity=48.3950617283951, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI261862346, Length=404, Percent_Identity=47.5247524752475, Blast_Score=358, Evalue=7e-99,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=49.8753117206983, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=48.1958762886598, Blast_Score=316, Evalue=2e-86,
Organism=Escherichia coli, GI1788902, Length=409, Percent_Identity=58.9242053789731, Blast_Score=473, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI25144729, Length=403, Percent_Identity=49.1315136476427, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25144732, Length=403, Percent_Identity=49.1315136476427, Blast_Score=375, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6319739, Length=422, Percent_Identity=46.4454976303318, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6323087, Length=420, Percent_Identity=46.6666666666667, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24640005, Length=410, Percent_Identity=49.5121951219512, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI221329721, Length=410, Percent_Identity=49.5121951219512, Blast_Score=387, Evalue=1e-108,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_EUBR3 (C4ZH69)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002937015.1
- ProteinModelPortal:   C4ZH69
- GeneID:   7964132
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_1119
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45135; Mature: 45135

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTLDDIKKEDPEIASAITDEFERQNSHIELIASENWVSPAVMSAMGSILTNKYAEGYPG
CCCCHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
RRYYGGCECVDEVEELARERAKELFGAEYVNVQPHSGAQANMAVQFAILKPGDTIMGMNL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCEEEEEEC
DHGGHLTHGSPVNFSGSYFHVVPYGVNDEGFIDYDKVEEIALECKPKMIIAGASAYARTI
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHH
DFKRFREIADKVDAVLMVDMAHIAGLVAAGLHPSPIPYAHVTTTTTHKTLRGPRGGMILC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHCCCCCCEEEE
SQEMQDKYNFNKAIFPGIQGGPLMHVIAAKAVCFKEALQPEFKEYQKQIVKNAQALCKGL
CHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
QSRGIKIVSDGTDNHLMLVDLTPFGLTGKSIEKLLDAAHITANKNTIPNDPQKPFVTSGI
HHCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCHHHCCE
RLGTPAATSRGLKEDDFDKVAEAIAMIIKEGESAVEPAKAIIKTLTDKYPLAF
ECCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYTLDDIKKEDPEIASAITDEFERQNSHIELIASENWVSPAVMSAMGSILTNKYAEGYPG
CCCCHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
RRYYGGCECVDEVEELARERAKELFGAEYVNVQPHSGAQANMAVQFAILKPGDTIMGMNL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCEEEEEEC
DHGGHLTHGSPVNFSGSYFHVVPYGVNDEGFIDYDKVEEIALECKPKMIIAGASAYARTI
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHH
DFKRFREIADKVDAVLMVDMAHIAGLVAAGLHPSPIPYAHVTTTTTHKTLRGPRGGMILC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHCCCCCCEEEE
SQEMQDKYNFNKAIFPGIQGGPLMHVIAAKAVCFKEALQPEFKEYQKQIVKNAQALCKGL
CHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
QSRGIKIVSDGTDNHLMLVDLTPFGLTGKSIEKLLDAAHITANKNTIPNDPQKPFVTSGI
HHCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCHHHCCE
RLGTPAATSRGLKEDDFDKVAEAIAMIIKEGESAVEPAKAIIKTLTDKYPLAF
ECCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA