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The map label for this gene is ngoPIIM [H]

Identifier: 238923038

GI number: 238923038

Start: 571852

End: 573870

Strand: Direct

Name: ngoPIIM [H]

Synonym: EUBREC_0628

Alternate gene names: 238923038

Gene position: 571852-573870 (Clockwise)

Preceding gene: 238923037

Following gene: 238923039

Centisome position: 16.58

GC content: 34.77

Gene sequence:

>2019_bases
ATGAACATAATTAGTTTGTTTAGTGGATGTGGGGGATTAGATTTAGGATTTGAAAGCGCTGGGTTTAATATTCCTGTTGC
TAATGAATTTGATAAGACAATATGGGCTACATTCAAAGCTAATCACCCTAATACACATCTGATTGAAGGAGATATAAGAC
AAGTAACAAAAGAAGATATTGAACAGTATATAGATGGTGAAATTGATGGAATTATAGGTGGACCACCTTGTCAGTCATGG
TCTGAAGCTGGATCATTGAAAGGTATTAAGGATGCAAGGGGACAGTTATTTTTTGATTATATAAGAATTCTTAAGGAATT
TCAGCCTAAATTCTTTCTGGCGGAAAATGTTAGCGGAATGTTAGCTAATAGACATTCAGAGGCAGTACAGAACATACTCA
ATCTTTTCGATGGAGCAGGATATGATGTATCTTTTACACTTGTTAATGCAAAGGATTATGGTGTGGCTGAAGAAAGAAAA
AGGGTATTTTATATAGGGTTTAGAAAAGACTTGAATATTGATTTTGGATTTCCTAAGGGGTCAACCAAAGAAGATGATAA
GAAGATTACTCTTAGAGATATAATATGGGATTTACAGGATACAGCAGTGCCTTCAGGAGAAAAAAATCATCATAATCCAG
AAGCAATCAATAATAATGAGTATTACACAGGAGCATATTCTCCAATTTTTATGAGCCGAAACAGAGTGAAATCCTGGGAT
GAGCAAGCTTTTACAGTACAGGCATCAGGAAGACAATGTCAGTTACATCCACAGGCACCTAAGATGGTTAAGGTGGGGCA
GAATGATTGCAGATTTGTAGAGGGCAAAGAACATCTTTATCGCCGAATGACAATAAGAGAAGTAGCCAGAGTTCAAGGGT
TTCCAGATAATTTTAAATTTATATATGAAGATACAAACACAGCTTATAAAATGATTGGAAATGCAGTTCCAGTTAATCTT
GCATATGAAATAGCAGTAGCAATAAAGAAATACTTAGAGGGTAATAGTGCAGATGTGGTAGTAGATGATGATGTTATAGA
TGCAAAGGAGGTCAATGAGAAAAAAGTGAGTACAAAGAGTAATGATCAGGGAAGAGCATATGAATATGCGTGGATTAAAA
CACTTTATAAGGCATTGTGTGAAATGCGTAAAACAAAAATAGTTGATAATTCCAGCTTACATGCGAATGAAAAAGCATGG
ATGCTTATGGATGAGGAAATGCAGCAGACATTTATGATTTCTGCTGAAGCTGCAATTAATGAAGTACTTGAAATGGAGCC
AAGATTATCAGAAAACGATAATGACGAACTGACATTAGAGTTTCAAAAAGATGGAGCTGGCGTTAAAGGCGATGTAAGAG
ATATTGTTATTCGCAGAGACGATATTGAATGGGAAATAGGATTAAGTATCAAGCACAATCACGATGCAGTTAAACACAGC
AGATTAAGTCATAAACTGGATTTTGGCAAAGAATGGTTTGATATACCTTGTAGTAACGAATATTGGGGTGCGGTTAATCC
AATATTTGATATGCTGAAAAGTGAAAAAGAAAATGGCTCACGATGGTCAGAAATAGTGCAGAAAGATGAAAATGTATATG
TACCATTATTGCAGGCTTTTATGGATGAAGTTAATAGGGCATATAAAGAAGATAAGAATATGCCTGAAAAGATGATAGAA
TACCTTATAGGAAAAGAAGATTATTACAAGATAGTTAGTCATGACAGTAAACGACTTACATTGATTCATACTTTTAATAT
GCATGATACTTTAAATAAGTCGAGTAAGGATAAGGTTTCGGAAATTGAAGTTCCTGTGGTTGAATTACCGACAAGACTGA
TTGACATTGGATTTAAGCCGAAAAGTAATAATACTGTAGAAATGATTTTGGACAATGGATGGCAGTTGAGTTTTAGAATT
CATAGTGCAAGTACAAAGGTAGAACCAAGTTTAAAATTCGATGTGCAGTTTATAAGTATGCCTGTGTCAGTATGCACTAT
CAAATGTGTATGGAAATAA

Upstream 100 bases:

>100_bases
AGACTGGAGGCATATGCTTCATATAGTGAGTTTGTTAGAAGAACGGCATTAATAGAAGCTACAAGAATAATCAAGGAAAA
CAATGAAGGAGAAAATACCT

Downstream 100 bases:

>100_bases
GGTGGTGCAAGTATTGAGAAAAATGTTGTTAAGCTTCAGACCAGATGTGTATGAAAAAATAAAATCTGGTCTGAAGATAT
TCGAACACAGAAGAAATTTT

Product: DcmB

Products: NA

Alternate protein names: M.NgoPII; Cytosine-specific methyltransferase NgoPII [H]

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MNIISLFSGCGGLDLGFESAGFNIPVANEFDKTIWATFKANHPNTHLIEGDIRQVTKEDIEQYIDGEIDGIIGGPPCQSW
SEAGSLKGIKDARGQLFFDYIRILKEFQPKFFLAENVSGMLANRHSEAVQNILNLFDGAGYDVSFTLVNAKDYGVAEERK
RVFYIGFRKDLNIDFGFPKGSTKEDDKKITLRDIIWDLQDTAVPSGEKNHHNPEAINNNEYYTGAYSPIFMSRNRVKSWD
EQAFTVQASGRQCQLHPQAPKMVKVGQNDCRFVEGKEHLYRRMTIREVARVQGFPDNFKFIYEDTNTAYKMIGNAVPVNL
AYEIAVAIKKYLEGNSADVVVDDDVIDAKEVNEKKVSTKSNDQGRAYEYAWIKTLYKALCEMRKTKIVDNSSLHANEKAW
MLMDEEMQQTFMISAEAAINEVLEMEPRLSENDNDELTLEFQKDGAGVKGDVRDIVIRRDDIEWEIGLSIKHNHDAVKHS
RLSHKLDFGKEWFDIPCSNEYWGAVNPIFDMLKSEKENGSRWSEIVQKDENVYVPLLQAFMDEVNRAYKEDKNMPEKMIE
YLIGKEDYYKIVSHDSKRLTLIHTFNMHDTLNKSSKDKVSEIEVPVVELPTRLIDIGFKPKSNNTVEMILDNGWQLSFRI
HSASTKVEPSLKFDVQFISMPVSVCTIKCVWK

Sequences:

>Translated_672_residues
MNIISLFSGCGGLDLGFESAGFNIPVANEFDKTIWATFKANHPNTHLIEGDIRQVTKEDIEQYIDGEIDGIIGGPPCQSW
SEAGSLKGIKDARGQLFFDYIRILKEFQPKFFLAENVSGMLANRHSEAVQNILNLFDGAGYDVSFTLVNAKDYGVAEERK
RVFYIGFRKDLNIDFGFPKGSTKEDDKKITLRDIIWDLQDTAVPSGEKNHHNPEAINNNEYYTGAYSPIFMSRNRVKSWD
EQAFTVQASGRQCQLHPQAPKMVKVGQNDCRFVEGKEHLYRRMTIREVARVQGFPDNFKFIYEDTNTAYKMIGNAVPVNL
AYEIAVAIKKYLEGNSADVVVDDDVIDAKEVNEKKVSTKSNDQGRAYEYAWIKTLYKALCEMRKTKIVDNSSLHANEKAW
MLMDEEMQQTFMISAEAAINEVLEMEPRLSENDNDELTLEFQKDGAGVKGDVRDIVIRRDDIEWEIGLSIKHNHDAVKHS
RLSHKLDFGKEWFDIPCSNEYWGAVNPIFDMLKSEKENGSRWSEIVQKDENVYVPLLQAFMDEVNRAYKEDKNMPEKMIE
YLIGKEDYYKIVSHDSKRLTLIHTFNMHDTLNKSSKDKVSEIEVPVVELPTRLIDIGFKPKSNNTVEMILDNGWQLSFRI
HSASTKVEPSLKFDVQFISMPVSVCTIKCVWK
>Mature_672_residues
MNIISLFSGCGGLDLGFESAGFNIPVANEFDKTIWATFKANHPNTHLIEGDIRQVTKEDIEQYIDGEIDGIIGGPPCQSW
SEAGSLKGIKDARGQLFFDYIRILKEFQPKFFLAENVSGMLANRHSEAVQNILNLFDGAGYDVSFTLVNAKDYGVAEERK
RVFYIGFRKDLNIDFGFPKGSTKEDDKKITLRDIIWDLQDTAVPSGEKNHHNPEAINNNEYYTGAYSPIFMSRNRVKSWD
EQAFTVQASGRQCQLHPQAPKMVKVGQNDCRFVEGKEHLYRRMTIREVARVQGFPDNFKFIYEDTNTAYKMIGNAVPVNL
AYEIAVAIKKYLEGNSADVVVDDDVIDAKEVNEKKVSTKSNDQGRAYEYAWIKTLYKALCEMRKTKIVDNSSLHANEKAW
MLMDEEMQQTFMISAEAAINEVLEMEPRLSENDNDELTLEFQKDGAGVKGDVRDIVIRRDDIEWEIGLSIKHNHDAVKHS
RLSHKLDFGKEWFDIPCSNEYWGAVNPIFDMLKSEKENGSRWSEIVQKDENVYVPLLQAFMDEVNRAYKEDKNMPEKMIE
YLIGKEDYYKIVSHDSKRLTLIHTFNMHDTLNKSSKDKVSEIEVPVVELPTRLIDIGFKPKSNNTVEMILDNGWQLSFRI
HSASTKVEPSLKFDVQFISMPVSVCTIKCVWK

Specific function: This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the NgoPII endonuclease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4503351, Length=189, Percent_Identity=25.3968253968254, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI195927037, Length=189, Percent_Identity=25.3968253968254, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788271, Length=379, Percent_Identity=27.4406332453826, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 76928; Mature: 76928

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIISLFSGCGGLDLGFESAGFNIPVANEFDKTIWATFKANHPNTHLIEGDIRQVTKEDI
CCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEECCHHHHHHHHHH
EQYIDGEIDGIIGGPPCQSWSEAGSLKGIKDARGQLFFDYIRILKEFQPKFFLAENVSGM
HHHHCCCCCEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHH
LANRHSEAVQNILNLFDGAGYDVSFTLVNAKDYGVAEERKRVFYIGFRKDLNIDFGFPKG
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHCCEEEEEEEEECCCEEECCCCC
STKEDDKKITLRDIIWDLQDTAVPSGEKNHHNPEAINNNEYYTGAYSPIFMSRNRVKSWD
CCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEECCCCCCCCC
EQAFTVQASGRQCQLHPQAPKMVKVGQNDCRFVEGKEHLYRRMTIREVARVQGFPDNFKF
CCEEEEEECCCEEEECCCCCCEEECCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCEE
IYEDTNTAYKMIGNAVPVNLAYEIAVAIKKYLEGNSADVVVDDDVIDAKEVNEKKVSTKS
EEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHCCHHHCCCCC
NDQGRAYEYAWIKTLYKALCEMRKTKIVDNSSLHANEKAWMLMDEEMQQTFMISAEAAIN
CCCCCEEHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECHHHHHHEEEEHHHHHH
EVLEMEPRLSENDNDELTLEFQKDGAGVKGDVRDIVIRRDDIEWEIGLSIKHNHDAVKHS
HHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEEEECCCHHHHHH
RLSHKLDFGKEWFDIPCSNEYWGAVNPIFDMLKSEKENGSRWSEIVQKDENVYVPLLQAF
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEHHHHHHH
MDEVNRAYKEDKNMPEKMIEYLIGKEDYYKIVSHDSKRLTLIHTFNMHDTLNKSSKDKVS
HHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCEEEEEEEECCHHHCCCCCCCCHH
EIEVPVVELPTRLIDIGFKPKSNNTVEMILDNGWQLSFRIHSASTKVEPSLKFDVQFISM
HCCCCHHHHCCHHEEECCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCEEEEEEEEEC
PVSVCTIKCVWK
CEEEEEEEEEEC
>Mature Secondary Structure
MNIISLFSGCGGLDLGFESAGFNIPVANEFDKTIWATFKANHPNTHLIEGDIRQVTKEDI
CCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEECCHHHHHHHHHH
EQYIDGEIDGIIGGPPCQSWSEAGSLKGIKDARGQLFFDYIRILKEFQPKFFLAENVSGM
HHHHCCCCCEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHH
LANRHSEAVQNILNLFDGAGYDVSFTLVNAKDYGVAEERKRVFYIGFRKDLNIDFGFPKG
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHCCEEEEEEEEECCCEEECCCCC
STKEDDKKITLRDIIWDLQDTAVPSGEKNHHNPEAINNNEYYTGAYSPIFMSRNRVKSWD
CCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEECCCCCCCCC
EQAFTVQASGRQCQLHPQAPKMVKVGQNDCRFVEGKEHLYRRMTIREVARVQGFPDNFKF
CCEEEEEECCCEEEECCCCCCEEECCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCEE
IYEDTNTAYKMIGNAVPVNLAYEIAVAIKKYLEGNSADVVVDDDVIDAKEVNEKKVSTKS
EEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHCCHHHCCCCC
NDQGRAYEYAWIKTLYKALCEMRKTKIVDNSSLHANEKAWMLMDEEMQQTFMISAEAAIN
CCCCCEEHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECHHHHHHEEEEHHHHHH
EVLEMEPRLSENDNDELTLEFQKDGAGVKGDVRDIVIRRDDIEWEIGLSIKHNHDAVKHS
HHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEEEECCCHHHHHH
RLSHKLDFGKEWFDIPCSNEYWGAVNPIFDMLKSEKENGSRWSEIVQKDENVYVPLLQAF
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEHHHHHHH
MDEVNRAYKEDKNMPEKMIEYLIGKEDYYKIVSHDSKRLTLIHTFNMHDTLNKSSKDKVS
HHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCEEEEEEEECCHHHCCCCCCCCHH
EIEVPVVELPTRLIDIGFKPKSNNTVEMILDNGWQLSFRIHSASTKVEPSLKFDVQFISM
HCCCCHHHHCCHHEEECCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCEEEEEEEEEC
PVSVCTIKCVWK
CEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2837733; 2501649; 8406039 [H]