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The map label for this gene is hup [H]

Identifier: 238922939

GI number: 238922939

Start: 488321

End: 488620

Strand: Direct

Name: hup [H]

Synonym: EUBREC_0527

Alternate gene names: 238922939

Gene position: 488321-488620 (Clockwise)

Preceding gene: 238922938

Following gene: 238922940

Centisome position: 14.16

GC content: 42.33

Gene sequence:

>300_bases
ATGAAAATCCAGAGGAGGAATTTTATCATGAACAAAGCAGATTTAGTTGCAGCTATGGCAGAGAAGGCCGGAGTATCAAA
GAAGGATGCAGAGGCATCTTTAAAGGCATTCACAGATGTTGTTGCAGAGGAGTTAAAGAAAGGTGAGAAGATCCAGTTAG
TTGGATTCGGTACTTTCGAGGTATCTGAGAGAGCAGCAAGAACAGGAAGAAATCCACAGACTGGCGCTGAGATGACAATT
GCAGCATCTAAGGCTCCTAAGTTCAAGGCTGGAAAGGCTTTAAAAGATTCTCTTAACTAA

Upstream 100 bases:

>100_bases
AAAGCCTAGGAAATAGCGCATTTTCCTTGACAAACCCCATAAAAACAAGTATATATATTCTTGTAGGTAATTTGGGAATA
CAGCTCGAATTATATAAATT

Downstream 100 bases:

>100_bases
TAAGTTAGACTATCACGGAGTAGAAGAATGGGTGAAGCCTTTAAGGCTCCGCCCATTTTTGGCTATATCCACTGAAATCA
GGGTAATGGTTATGAATTGA

Product: DNA-binding protein HU

Products: NA

Alternate protein names: DNA-binding protein II; HB [H]

Number of amino acids: Translated: 99; Mature: 99

Protein sequence:

>99_residues
MKIQRRNFIMNKADLVAAMAEKAGVSKKDAEASLKAFTDVVAEELKKGEKIQLVGFGTFEVSERAARTGRNPQTGAEMTI
AASKAPKFKAGKALKDSLN

Sequences:

>Translated_99_residues
MKIQRRNFIMNKADLVAAMAEKAGVSKKDAEASLKAFTDVVAEELKKGEKIQLVGFGTFEVSERAARTGRNPQTGAEMTI
AASKAPKFKAGKALKDSLN
>Mature_99_residues
MKIQRRNFIMNKADLVAAMAEKAGVSKKDAEASLKAFTDVVAEELKKGEKIQLVGFGTFEVSERAARTGRNPQTGAEMTI
AASKAPKFKAGKALKDSLN

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=58.8888888888889, Blast_Score=104, Evalue=1e-24,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=55.0561797752809, Blast_Score=100, Evalue=1e-23,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=37.3626373626374, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI1788005, Length=86, Percent_Identity=34.8837209302326, Blast_Score=69, Evalue=7e-14,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 10657; Mature: 10657

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQRRNFIMNKADLVAAMAEKAGVSKKDAEASLKAFTDVVAEELKKGEKIQLVGFGTFE
CCCCCCHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHH
VSERAARTGRNPQTGAEMTIAASKAPKFKAGKALKDSLN
HHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCC
>Mature Secondary Structure
MKIQRRNFIMNKADLVAAMAEKAGVSKKDAEASLKAFTDVVAEELKKGEKIQLVGFGTFE
CCCCCCHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHH
VSERAARTGRNPQTGAEMTIAASKAPKFKAGKALKDSLN
HHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1644313 [H]